TRANSCRIPTION FACTOR DOSAGE AND GENETIC BACKGROUND IN MOUSE GONADAL SEX DETERMINATION: A CRITICAL ROLE FOR Fog2 AND Gata4 IN FETAL TESTIS DEVELOPMENT

Abstract GH plays a central role in controlling somatic growth, tissue regeneration, and intermediary metabolism in most vertebrate species through mechanisms dependent on the regulation of gene expression. Recent studies using transcript profiling have identified large cohorts of genes whose expression is induced by GH. Other results have demonstrated that signal transducer and activator of transcription (Stat) 5b, a latent transcription factor activated by the GH receptor-associated protein kinase, Jak2, is a key agent in the GH-stimulated gene activation that leads to somatic growth. By contrast, little is known about the steps through which GH-initiated signaling pathways reduce gene expression. Here we show that Stat5b plays a critical role in the GH-regulated inhibition of IGF binding protein-1 gene transcription by impairing the actions of the FoxO1 transcription factor on the IGF binding protein-1 promoter. Additional observations using transcript profiling in the liver indicate that Stat5b may be a general mediator of GH-initiated gene repression. Our results provide a model for understanding how GH may simultaneously stimulate and inhibit the expression of different cohorts of genes via the same transcription factor, potentially explaining how GH action leads to integrated biological responses in the whole organism.

Download Full-text

The molecular biology of SRY and its role in sex determination in mammals

Reproduction Fertility and Development ◽

10.1071/rd9950713 ◽

1995 ◽

Vol 7 (4) ◽

pp. 713 ◽

Cited By ~ 24

Author(s):

P Koopman

Keyword(s):

Molecular Biology ◽

Transgenic Mice ◽

Sex Determination ◽

Gene Product ◽

Mode Of Action ◽

Recent Progress ◽

Sry Gene ◽

Testis Development ◽

Direct Demonstration

SRY is the gene that initiates the genetic cascade leading to testis development in mammals. Since its discovery in 1990 and the direct demonstration of its male-determining role in transgenic mice, attention has turned to understanding the biochemical mode of action of the SRY gene product, and to the identification of other genes in the sex-determining pathway. Recent progress in these efforts is summarized in this review.

Download Full-text

Heat-shock transcription factor HSF1 has a critical role in human epidermal growth factor receptor-2-induced cellular transformation and tumorigenesis

Oncogene ◽

10.1038/onc.2010.277 ◽

2010 ◽

Vol 29 (37) ◽

pp. 5204-5213 ◽

Cited By ~ 89

Author(s):

L Meng ◽

V L Gabai ◽

M Y Sherman

Keyword(s):

Transcription Factor ◽

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Heat Shock ◽

Epidermal Growth Factor ◽

Critical Role ◽

Growth Factor Receptor ◽

Cellular Transformation ◽

Human Epidermal Growth Factor ◽

Epidermal Growth

Download Full-text

In utero exposure to hexavalent chromium disrupts rat fetal testis development

Toxicology Letters ◽

10.1016/j.toxlet.2018.10.010 ◽

2018 ◽

Vol 299 ◽

pp. 201-209 ◽

Cited By ~ 3

Author(s):

Wenwen Zheng ◽

Fei Ge ◽

Keyang Wu ◽

Xianwu Chen ◽

Xiaoheng Li ◽

...

Keyword(s):

Hexavalent Chromium ◽

In Utero ◽

In Utero Exposure ◽

Testis Development ◽

Fetal Testis

Download Full-text

The THO/TREX Complex Component RAE2/TEX1 Is Involved in the Regulation of Aluminum Resistance and Low Phosphate Response in Arabidopsis

Frontiers in Plant Science ◽

10.3389/fpls.2021.698443 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yi-Fang Zhu ◽

Jinliang Guo ◽

Yang Zhang ◽

Chao-Feng Huang

Keyword(s):

Arabidopsis Thaliana ◽

Transcription Factor ◽

Mrna Export ◽

Critical Role ◽

Core Component ◽

Mrna Accumulation ◽

Zinc Finger Transcription Factor ◽

Phosphate Response ◽

Encoding Gene ◽

Malate Transporter

The C2H2-type zinc finger transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) plays a critical role in aluminum (Al) resistance and low phosphate (Pi) response mainly through promoting the expression of the malate transporter-encoding gene ARABIDOPSIS THALIANA ALUMINUM ACTIVATED MALATE TRANSPORTER 1 (AtALMT1). We previously showed that REGULATION OF ATALMT1 EXPRESSION 3 (RAE3/HPR1), a core component of the THO/TREX complex, is involved in the regulation of nucleocytoplasmic STOP1 mRNA export to modulate Al resistance and low Pi response. Here, we report that RAE2/TEX1, another core component of the THO complex, is also involved in the regulation of Al resistance and low Pi response. Mutation of RAE2 reduced the expression of STOP1-downstream genes, including AtALMT1. rae2 was less sensitive to Al than rae3, which was consistent with less amount of malate secreted from rae3 roots than from rae2 roots. Nevertheless, low Pi response was impaired more in rae2 than in rae3, suggesting that RAE2 also regulates AtALMT1-independent pathway to modulate low Pi response. Furthermore, unlike RAE3 that regulates STOP1 mRNA export, mutating RAE2 did not affect STOP1 mRNA accumulation in the nucleus, although STOP1 protein level was reduced in rae2. Introduction of rae1 mutation into rae2 mutant background could partially recover the deficient phenotypes of rae2. Together, our results demonstrate that RAE2 and RAE3 play overlapping but distinct roles in the modulation of Al resistance and low Pi response.

Download Full-text

The Halophyte Halostachys caspica AP2/ERF Transcription Factor HcTOE3 Positively Regulates Freezing Tolerance in Arabidopsis

Frontiers in Plant Science ◽

10.3389/fpls.2021.638788 ◽

2021 ◽

Vol 12 ◽

Author(s):

Fangliu Yin ◽

Youling Zeng ◽

Jieyun Ji ◽

Pengju Wang ◽

Yufang Zhang ◽

...

Keyword(s):

Transcription Factor ◽

Abscisic Acid ◽

Freezing Tolerance ◽

Critical Role ◽

Extreme Temperature ◽

Freezing Stress ◽

Antioxidant Enzyme Activities ◽

Transcription Factor Gene ◽

Factor Gene ◽

Halostachys Caspica

The APETALA2 (AP2) and ethylene-responsive element-binding factor (ERF) gene family is one of the largest plant-specific transcription factor gene families, which plays a critical role in plant development and evolution, as well as response to various stresses. The TARGET OF EAT3 (TOE3) gene is derived from Halostachys caspica and belongs to the AP2 subfamily with two AP2 DNA-binding domains. Currently, AP2 family mainly plays crucial roles in plant growth and evolution, yet there are few reports about the role of AP2 in abiotic stress tolerance. Here, we report HcTOE3, a new cold-regulated transcription factor gene, which has an important contribution to freezing tolerance. The main results showed that the expression of HcTOE3 in the H. caspica assimilating branches was strongly induced by different abiotic stresses, including high salinity, drought, and extreme temperature (heat, chilling, and freezing), as well as abscisic acid and methyl viologen treatments. Overexpressing HcTOE3 gene (OE) induced transgenic Arabidopsis plant tolerance to freezing stress. Under freezing treatment, the OE lines showed lower content of malondialdehyde and electrolyte leakage and less accumulation of reactive oxygen species compared with the wild type. However, the survival rates, antioxidant enzyme activities, and contents of osmotic adjustment substance proline were enhanced in transgenic plants. Additionally, the OE lines increased freezing tolerance by up-regulating the transcription level of cold responsive genes (CBF1, CBF2, COR15, COR47, KIN1, and RD29A) and abscisic acid signal transduction pathway genes (ABI1, ABI2, ABI5, and RAB18). Our results suggested that HcTOE3 positively regulated freezing stress and has a great potential as a candidate gene to improve plant freezing tolerance.

Download Full-text