scholarly journals Exploring the possibilities of infrared spectroscopy for urine sediment examination and detection of pathogenic bacteria in urinary tract infections

2020 ◽  
Vol 58 (10) ◽  
pp. 1759-1767
Author(s):  
Mieke Steenbeke ◽  
Sander De Bruyne ◽  
Jerina Boelens ◽  
Matthijs Oyaert ◽  
Griet Glorieux ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Agar Plate ◽  
Principal Component ◽  
Blood Agar Plate ◽  
Urine Sediment ◽  
Flight Mass Spectrometry ◽  
Tract Infections

AbstractObjectivesIn this study, the possibilities of Fourier-transformed infrared spectroscopy (FTIR) for analysis of urine sediments and for detection of bacteria causing urinary tract infections (UTIs) were investigated.MethodsDried urine specimens of control subjects and patients presenting with various nephrological and urological conditions were analysed using mid-infrared spectroscopy (4,000–400 cm−1). Urine samples from patients with a UTI were inoculated on a blood agar plate. After drying of the pure bacterial colonies, FTIR was applied and compared with the results obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Chemometric data analysis was used to classify the different species.ResultsDue to the typical molecular assignments of lipids, proteins, nucleic acids and carbohydrates, FTIR was able to identify bacteria and showed promising results in the detection of proteins, lipids, white and red blood cells, as well as in the identification of crystals. Principal component analysis (PCA) allowed to differentiate between Gram-negative and Gram-positive species and soft independent modelling of class analogy (SIMCA) revealed promising classification ratios between the different pathogens.ConclusionsFTIR can be considered as a supplementary method for urine sediment examination and for detection of pathogenic bacteria in UTI.

Urinary tract infections in febrile children: Changing spectra of pathogenic bacteria and antibiotic susceptibilities?

2018 ◽  
Vol 55 (6) ◽  
pp. 680-689
Author(s):  
Jus Rakhra ◽  
Gabrielle Williams ◽  
Ben J Marais ◽  
Jonathan C Craig ◽  
Hasantha Gunasekera
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Antibiotic Susceptibilities ◽  
Tract Infections

scholarly journals Selective isolation and eradication of E. coli associated with urinary tract infections using anti-fimbrial modified magnetic reduced graphene oxide nanoheaters

2017 ◽  
Vol 5 (40) ◽  
pp. 8133-8142 ◽  
Author(s):  
Fatima Halouane ◽  
Roxana Jijie ◽  
Dalila Meziane ◽  
Chengnan Li ◽  
Santosh K. Singh ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Selective Isolation ◽  
E Coli ◽  
Reduced Graphene ◽  
Tract Infections ◽  
Efficient Elimination ◽  
Magnetic Reduced Graphene Oxide

The fast and efficient elimination of pathogenic bacteria from water, food or biological samples such as blood remains a challenging task.

Evaluation of urine specific gravity and urine sediment as risk factors for urinary tract infections in cats

2008 ◽  
Vol 37 (3) ◽  
pp. 317-322 ◽  
Author(s):  
Nathan L. Bailiff ◽  
Jodi L. Westropp ◽  
Richard W. Nelson ◽  
Jane E. Sykes ◽  
Sean D. Owens ◽  
...  
Keyword(s):  
Risk Factors ◽  
Urinary Tract ◽  
Specific Gravity ◽  
Urinary Tract Infections ◽  
Urine Specific Gravity ◽  
Urine Sediment ◽  
Tract Infections

scholarly journals Chemical profiling and antioxidant activity of Equisetum ramosissimum Desf. stem extract, a potential traditional medicinal plant for urinary tract infections

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jeyalatchagan Sureshkumar ◽  
Singamoorthy Amalraj ◽  
Ramar Murugan ◽  
Anantharaj Tamilselvan ◽  
Jayaraj Krupa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Urinary Tract ◽  
Medicinal Plant ◽  
Petroleum Ether ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Chemical Profiling ◽  
Stem Extract ◽  
Tract Infections

Abstract Background Equisetum ramosissimum Desf. (E. ramosissimum) is a widely used traditional medicinal plant to treat urinary tract infections (UTIs) by ethnic people throughout the world. The utility of the plant in treating urinary-related disorders was evaluated against selected pathogenic bacteria which has major role in causing UTIs. Hence, the present study executed to extract phytochemicals like total phenolics and flavonoids, chemical profiling by GC–MS analysis and to test their antioxidant activity from stem extracts of E. ramosissimum. The extraction process was directed by petroleum ether, chloroform, ethyl acetate, methanol, and aqueous solvents. Results The GC–MS analysis yielded 24 phytoconstituents with linoleic acid, palmitic acid, nonacosane, hexahydrofarnesyl acetone, and octacosane as major compounds. Methanolic extract yielded maximum amount of phenolics (TPC) and flavonoids (TFC) with 600.02 ± 0.22 mg GAE/g and 631.38 ± 0.69 mg QE/g, respectively. Methanolic extract also exhibited notable free radical scavenging activity with an IC50 of 123.89 ± 0.73, 150.10 ± 1.02, 146.01 ± 0.54, and 63.73 ± 6.12 µg/mL for DPPH, FRAP, ABTS, and O2− assays, respectively. The minimum inhibitory concentration (MIC) required to inhibit the growth of tested pathogenic bacteria was observed in aqueous and methanolic extracts with the value being 31.25 µg/mL against R. equi and V. cholerae. As like, methanolic and petroleum ether extracts efficiently inhibited the growth of B. subtilis with the MIC of 31.25 µg/mL. Conclusion It was concluded that the notable effect of methanolic and aqueous extracts against the uropathogenic bacteria reported in this study supported the traditional uses of this plant in treating UTIs. The results acquired from this investigation revealed that E. ramosissimum stem extract might be considered as an interesting candidate in the development of antibacterial agent against UTIs coupled with antioxidant properties.

scholarly journals Isolation and Identification of Microbes on Hands and Mobile Phones Causing Urinary Tract Infections

2021 ◽  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Mobile Phones ◽  
Pathogenic Bacteria ◽  
University Hospital ◽  
Isolation And Identification ◽  
Civil Hospital ◽  
Transmission Cycle ◽  
Tract Infections

Background: Hands and cell phones are the major source of cross-transmission of urinary tract infections. The aim of this study was to isolate, identify and evaluate Gram-negative bacteria from hand and mobile phones. Methods: This study was conducted in visiting area of Civil Hospital Karachi, Pakistan. Analysis was done by 100 wet sterile cotton tipped swabs, 50 each from mobile phones and hands of their owners. Samples were transported in a Cary Blair transport media, Swabs were streaked on Nutrient agar, Blood agar and MacConkey agar. Organisms were identified by cultural, biochemical, and microscopic characteristics. Results: Total samples n=100 was collected from hand and mobile phones samples (50 hand and 50 mobile swabs) from the Dow university Hospital and Civil Hospital Karachi were tested. Six species of bacteria were isolated along with their identification during the research study. The isolated bacteria were Serratia, Klebsiella, Pseudomonas, Proteus, Shigella and Escherichia coli. The participants’ hands showed high bacterial contamination (50%-56%) in comparison to mobile phones. The frequency (%) of bacteria isolated from mobile phone and hand swabs included Serratia, 12 (24%) with the highest quantity and frequently found bacteria. While, the rest of the results reported Escherichia coli 10 (20%), Klebsiella 9 (18%), Pseudomonas 5 (10%), Shigella 4 (8%) and Proteus 10 (20%) respectively. Conclusion: Patient attendants in hospitals and visitors are more susceptible to nosocomial infections through exchange of mobile phones n=12(24%). Therefore, hygienic practice of hands cleaning while mobile using may help to break the transmission cycle of pathogenic bacteria.

scholarly journals Adaptation in a Fibronectin Binding Autolysin of Staphylococcus saprophyticus

mSphere ◽  
2017 ◽  
Vol 2 (6) ◽  
Author(s):  
Tatum D. Mortimer ◽  
Douglas S. Annis ◽  
Mary B. O’Neill ◽  
Lindsey L. Bohr ◽  
Tracy M. Smith ◽  
...  
Keyword(s):  
Public Health ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Human Microbiome ◽  
Invasive Disease ◽  
Human Infection ◽  
Content Type ◽  
Staphylococcus Saprophyticus ◽  
Tract Infections

ABSTRACT Staphylococcus saprophyticus is an important cause of urinary tract infections (UTI) in women; such UTI are common, can be severe, and are associated with significant impacts to public health. In addition to being a cause of human UTI, S. saprophyticus can be found in the environment, in food, and associated with animals. After discovering that UTI strains of S. saprophyticus are for the most part closely related to each other, we sought to determine whether these strains are specially adapted to cause disease in humans. We found evidence suggesting that a mutation in the gene aas is advantageous in the context of human infection. We hypothesize that the mutation allows S. saprophyticus to survive better in the human urinary tract. These results show how bacteria found in the environment can evolve to cause disease. Human-pathogenic bacteria are found in a variety of niches, including free-living, zoonotic, and microbiome environments. Identifying bacterial adaptations that enable invasive disease is an important means of gaining insight into the molecular basis of pathogenesis and understanding pathogen emergence. Staphylococcus saprophyticus, a leading cause of urinary tract infections, can be found in the environment, food, animals, and the human microbiome. We identified a selective sweep in the gene encoding the Aas adhesin, a key virulence factor that binds host fibronectin. We hypothesize that the mutation under selection (aas_2206A>C) facilitates colonization of the urinary tract, an environment where bacteria are subject to strong shearing forces. The mutation appears to have enabled emergence and expansion of a human-pathogenic lineage of S. saprophyticus. These results demonstrate the power of evolutionary genomic approaches in discovering the genetic basis of virulence and emphasize the pleiotropy and adaptability of bacteria occupying diverse niches. IMPORTANCE Staphylococcus saprophyticus is an important cause of urinary tract infections (UTI) in women; such UTI are common, can be severe, and are associated with significant impacts to public health. In addition to being a cause of human UTI, S. saprophyticus can be found in the environment, in food, and associated with animals. After discovering that UTI strains of S. saprophyticus are for the most part closely related to each other, we sought to determine whether these strains are specially adapted to cause disease in humans. We found evidence suggesting that a mutation in the gene aas is advantageous in the context of human infection. We hypothesize that the mutation allows S. saprophyticus to survive better in the human urinary tract. These results show how bacteria found in the environment can evolve to cause disease.

scholarly journals Species distribution of Enterobacteriaceae and non-Enterobacteriaceae responsible for urinary tract infections at the Zainoel Abidin Hospital in Banda Aceh, Indonesia

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Suhartono Suhartono ◽  
Wilda Mahdani ◽  
Zinatul Hayati ◽  
NURHALIMAH NURHALIMAH
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Species Distribution ◽  
Pathogenic Bacteria ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Vaginal Mucosa ◽  
Gram Positive ◽  
Tract Infections ◽  
Banda Aceh

Abstract. Suhartono S, Mahdani W, Hayati Z, Nurhalimah N. 2021. Species distribution of Enterobacteriaceae and non-Enterobacteriaceae responsible for urinary tract infections at the Zainoel Abidin Hospital in Banda Aceh, Indonesia. Biodiversitas 22: 3313-3318. Urinary tract infection (UTI) is an infection that occurs along the urinary tract caused by pathogenic bacteria. Enterobacteriaceae and non-Enterobacteriaceae are generally normal flora originating from the large intestine and vaginal mucosa. This study aimed to assess the distribution of Enterobacteriaceae and non -Enterobacteriaceae pathogens that cause UTI based on species diversity, patient age, gender, and hospital units, and to evaluate their antibiotic sensitivity to uropathogens in ZAH. In total, there were 284 isolates classified as UTI pathogens with 178 Enterobacteriaceae isolates dominated by Escherichia coli and 107 non- Enterobacteriaceae isolates. Non- Enterobacteriaceae consisted of 77 isolates of Gram-positive dominated by Enterococcus faecalis and Enterococcus facium, and 29 isolates of Gram-negative dominated by Acinetobacter baumannii. The highest UTI ratio was found in the elderly (56-65 years), namely 66.2% Enterobacteriaceae and 33.8% non-Enterobacteriaceae isolates. Based on gender, females were infected with Enterobacteriaceae (66.86%) and non-Enterobacteriaceae (37.41%). Most of UTI patients in the internal medicine unit were infected with Enterobacteriaceae (55.36%) and non-Enterobacteriaceae (44.64%). Amikacin, meropenem, and levofloxacin were antibiotics that had high sensitivity against Enterobacterial and non-Enterobacterial uropathogens. The emergence of Gram-positive uropathogens need to be concerned as these groups start emerging. It is also important to monitor regularly the antibiotic susceptibility of bacterial UTI pathogens to ensure the efficacy of current UTI treatments and minimize the UTI incidence in nosocomial settings.

scholarly journals Pathogenetic Role and Possibilities for Correction of Gut Microbiota Disorders in Urinary Tract Infections

2021 ◽  
Vol 66 (7-8) ◽  
pp. 100-108
Author(s):  
N. V. Sturov ◽  
S. V. Popov ◽  
V. A. Zhukov
Keyword(s):  
Urinary Tract ◽  
Gut Microbiota ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Fecal Microbiota Transplantation ◽  
Evidence Base ◽  
Fecal Microbiota ◽  
Current Data ◽  
Treatment And Prevention ◽  
Tract Infections

The article presents current data on the role of gut microbiota in the development of urinary tract infections. The main pathogenetic mechanisms contributing to the chronic recurrent course of the disease, the spread of antimicrobial resistance, as well as their connection to the disorders of gut microbiota are shown. It is known that most pathogens of urinary infections originate from the gut microbiota, where they exist for a long time, forming reservoirs. The normal composition and functions of the microbiota prevent colonization of the intestine by pathogenic bacteria and reduce the risk of developing this disease. Ways of correction through diet, probiotics, as well as fecal microbiota transplantation are considered. Modulation of gut microbiota may be a promising approach in the treatment and prevention of urinary tract infections. Meanwhile, a qualitative evidence base on the effectiveness of this strategy has not been formed. Further research in this direction is required.

Assessment the Activity of Some Enzymes and Antibiotic Substances Sensitivity on Pathogenic Bacteria Species

2018 ◽  
Vol 68 (12) ◽  
pp. 3015-3021 ◽  
Author(s):  
Ionela Sarbu ◽  
Tatiana Vassu ◽  
Mariana Carmen Chifiriuc ◽  
Marcela Bucur ◽  
Ileana Stoica ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Bacterial Vaginosis ◽  
Urinary Tract Infections ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
High Ability ◽  
Opportunistic Pathogens ◽  
Microbial Strains ◽  
Phenotypic Methods ◽  
Tract Infections

Microbial and opportunistic pathogens cause serious problem to human host by releasing different compounds which are involved in colonization, invasion or in alteration of immune process. In this study we propose ourselves to analyze the virulence profile of microorganisms involved in urinary tract infections and bacterial vaginosis in order to compare the virulence mechanisms developed by these microorganisms. We assessed the virulence profile (motility, adhesion to HeLa cell line, biofilm formation, production of enzymes, antibiotic susceptibility) using phenotypic methods. The microbial strains isolated from urinary tract infections present a high ability to release amylases, caseinase, siderophore-like, half of them have present high motility and also were highly resistant to antibiotics, while the microbial strains isolated from bacterial vaginosis present a high ability to bind human epithelial cells and to release hemolyzin and DN-ase.

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