scholarly journals The Effect of Choline on DNA Methylation in the Hippocampus of the Offspring of Gestational Diabetes Mellitus (GDM) Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1252-1252
Author(s):  
Kaydine Edwards ◽  
Xinyin Jiang ◽  
Hunter Korsmo
Keyword(s):  
Dna Methylation ◽  
Group Versus ◽  
Control Group ◽  
Global Dna Methylation ◽  
High Fat ◽  
Methylation Analysis ◽  
Feeding Condition ◽  
Control Groups ◽  
Funding Sources ◽  
Dna Methylation Analysis

Abstract Objectives The objective of this project is to determine the effects of choline supplementation on DNA methylation in the hippocampus of the offspring from mouse dams with GDM. Methods In this study, female mice were divided into four groups: high fat (HF) feeding (to induce GDM), HF with choline supplementation, normal fat (NF) control and NF with choline supplementation. The experimental groups followed their diets and supplements for 4 weeks before timed mating and throughout gestation. Thereafter, they were fed the NF diet during lactation. After weaning, the offspring were fed the HF diet for 6 weeks before dissection. Brain hippocampus was then dissected for DNA extraction and global DNA methylation analysis. Results Global DNA methylation was increased in the NF choline supplemented group versus the NF control group (P = 0.056); however, there were no differences between the HF choline versus the HF or NF control groups (P = 0.992). Conclusions In summary, there was an interaction between maternal HF feeding and choline supplementation in influencing global DNA methylation. Maternal HF feeding eliminated the increase in offspring hippocampal DNA methylation by choline supplementation observed under the NF feeding condition. Funding Sources NIGMS.

scholarly journals Integration of Genome-Wide DNA Methylation and Transcription Uncovered Aberrant Methylation-Regulated Genes and Pathways in the Peripheral Blood Mononuclear Cells of Systemic Sclerosis

2018 ◽  
Vol 2018 ◽  
pp. 1-19 ◽  
Author(s):  
Honglin Zhu ◽  
Chengsong Zhu ◽  
Wentao Mi ◽  
Tao Chen ◽  
Hongjun Zhao ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Systemic Sclerosis ◽  
Differentially Expressed ◽  
Unknown Etiology ◽  
Global Dna Methylation ◽  
Aberrant Methylation ◽  
Methylation Analysis ◽  
Regulatory Pathways ◽  
Genome Wide ◽  
Dna Methylation Analysis

Objective. Systemic sclerosis (SSc) is a systemic connective tissue disease of unknown etiology. Aberrant gene expression and epigenetic modifications in circulating immune cells have been implicated in the pathogenesis of SSc. This study is to delineate the interaction network between gene transcription and DNA methylation in PBMC of SSc patients and to identify methylation-regulated genes which are involved in the pathogenesis of SSc. Methods. Genome-wide mRNA transcription and global DNA methylation analysis were performed on PBMC from 18 SSc patients and 19 matched normal controls (NC) using Illumina BeadChips. Differentially expressed genes (DEGs) and differentially methylated positions (DMPs) were integrative analyzed to identify methylation-regulated genes and associated molecular pathways. Results. Transcriptome analysis distinguished 453 DEGs (269 up- and 184 downregulated) in SSc from NC. Global DNA methylation analysis identified 925 DMPs located on 618 genes. Integration of the two lists revealed only 20 DEGs which harbor inversely correlated DMPs, including 12 upregulated (ELANE, CTSG, LTBR, C3AR1, CSTA, SPI1, ODF3B, SAMD4A, PLAUR, NFE2, ZYX, and CTSZ) and eight downregulated genes (RUNX3, PRF1, PRKCH, PAG1, RASSF5, FYN, CXCR6, and F2R). These potential methylation-regulated DEGs (MeDEGs) are enriched in the pathways related to immune cell migration, proliferation, activation, and inflammation activities. Using a machine learning algorism, we identified six out of the 20 MeDEGs, including F2R, CXCR6, FYN, LTBR, CTSG, and ELANE, which distinguished SSc from NC with 100% accuracy. Four genes (F2R, FYN, PAG1, and PRKCH) differentially expressed in SSc with interstitial lung disease (ILD) compared to SSc without ILD. Conclusion. The identified MeDEGs may represent novel candidate factors which lead to the abnormal activation of immune regulatory pathways in the pathogenesis of SSc. They may also be used as diagnostic biomarkers for SSc and clinical complications.

scholarly journals 349 GLOBAL DNA METHYLATION ANALYSIS IN OSTEOARTHRITIS SYNOVIAL FIBROBLASTS

2011 ◽  
Vol 19 ◽  
pp. S158
Author(s):  
J.-Y. Choe ◽  
S.-H. Park ◽  
S.-K. Kim ◽  
H.-Y. Jung ◽  
H.-J. Lee ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Synovial Fibroblasts ◽  
Global Dna Methylation ◽  
Methylation Analysis ◽  
Dna Methylation Analysis

scholarly journals Global DNA Methylation Analysis Identifies Two Discrete clusters of Pheochromocytoma with Distinct Genomic and Genetic Alterations

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Samuel Backman ◽  
Rajani Maharjan ◽  
Alberto Falk-Delgado ◽  
Joakim Crona ◽  
Kenko Cupisti ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Genetic Alterations ◽  
Global Dna Methylation ◽  
Methylation Analysis ◽  
Dna Methylation Analysis

scholarly journals Recurrent Water Deficit and Epigenetic Memory in Medicago sativa L. Varieties

2020 ◽  
Vol 10 (9) ◽  
pp. 3110
Author(s):  
Yannis E. Ventouris ◽  
Eleni Tani ◽  
Evangelia V. Avramidou ◽  
Eleni M. Abraham ◽  
Styliani N. Chorianopoulou ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Drought Stress ◽  
Phase I ◽  
Phase Ii ◽  
Dry Weight ◽  
Global Dna Methylation ◽  
Methylation Analysis ◽  
Total Dna ◽  
Medicago Sativa L ◽  
Dna Methylation Analysis

Global DNA methylation changes in response to recurrent drought stress were investigated in two common Greek Medicago sativa L. varieties (Lamia and Chaironia-Institute of Ιndustrial and Forage Crops). The water deficit was implemented in two phases. At the end of the first phase, which lasted for 60 days, the plants were cut at the height of 5 cm and were watered regularly for two months before being subjected to the second drought stress, which lasted for two weeks. Finally, the following groups of plants were formed: CC (controls both in phase I and phase II), CD2 (Controls in phase I, experiencing drought in phase II), and D1D2 (were subjected to drought in both phase I and phase II). At the end of phase II, samples were taken for global DNA methylation analysis with the Methylation Sensitive Amplification Polymorphism (MSAP) method, and all plants were harvested in order to measure the fresh and dry weight of roots and shoots. The variety Lamia responded better, especially the D1D2 group, compared to Chaironia in terms of root and shoot dry weight. Additionally, the shoots of Lamia had a constant water status for CD2 and D1D2 group of plants. According to DNA methylation analysis by the MSAP method, Lamia had lower total DNA methylation percentage after the second drought episode (D1D2) as compared to the plants CD2 that had experienced only one drought episode. On the other hand, the total DNA methylation percentage of Chaironia was almost the same in plants grown under recurrent drought stress conditions compared to control plants. In conclusion, the decrease of DNA methylation of Lamia stressed plants probably indicates the existence of an epigenetic mechanism that may render drought tolerance.

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