scholarly journals Helicobacter Pylori Infection and Vitamin B12 Deficiency During Early Pregnancy in an Urban Slum in Bangladesh (P24-035-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Towfida Siddiqua ◽  
Md Nure Alam ◽  
Shaikh Meshbahuddin Ahmad ◽  
Razoanul Haque ◽  
Tahmeed Ahmed ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Vitamin B12 ◽  
Early Pregnancy ◽  
Controlled Trial ◽  
Urban Slum ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
B12 Deficiency ◽  
The Mean ◽  
H Pylori

Abstract Objectives Helicobacter pylori (H. pylori), an inflammatory gastric bacterial infection is prevalent worldwide and may impair the absorption of many micronutrients, particularly iron and vitamin B12. We measured H. pylori infection status in the extracted stool sample in pregnant women and examined its associations with biomarkers of vitamin B12 (B12) status. Methods In a blinded-placebo controlled trial, Bangladeshi women (n = 160, 18–35 years, gestational weeks 9–14, Hb > 70 g/L) were randomized to receive either B12 (100 μg/day) fortified or control milk throughout pregnancy up to 6-mo postpartum. Baseline data of the trial were used to conduct this analysis. Blood and stool samples were collected from all participants at enrollment. Folate, B12 and total homocysteine (tHcy) in plasma were measured by electrochemiluminescence immunoassay. Plasma active-B12 was determined by enzyme linked immunosorbent assay and H. pylori antigen in feces was measured by enzyme immunoassay. Results The mean hemoglobin concentration of women was 116.5 ± 10.2 g/L and 25.6% had anemia (Hb < 110 g/L). About 6.25% of the women were B12 deficient (150 pmol/L) and 29.38% had marginal status (150–220 pmol/L). The mean active B12 concentration was 43.3 ± 22.4 pmol/L and 39.38% (<35 pmol/L) were at risk of B12 deficiency. Elevated tHcy was found in 34.1% of the women (>8 μmol/L) and 3.8% had low plasma folate (<3.1 ng/mL). The majority of the participants (65%) had fecal H. pylori antigen, indicating asymptomatic carriage state. Presence of H. pylori antigen was accompanied by B12 deficiency in ̴23% of the women. Logistic regression analysis revealed that the odds of having H. pylori antigen was lower in women with increasing age [OR: 0.89 (95% CI: 0.81, 0.96); p-value = 0.005]. However, the results showed no significant association between B12 markers and H. pylori infection. Conclusions The high prevalence of H. pylori infection co-exists with micronutrient deficiencies during early pregnancy in an urban slum in Bangladesh, meriting more comprehensive approaches for their evaluation and prevention. Funding Sources Nestle Foundation.

scholarly journals Atorvastatin in Helicobacter Pylori Treatment: A Randomized Controlled Trial and Review

2021 ◽  
Author(s):  
Mohammad Reza Mohammad Hoseini Azar ◽  
Parham Porteghali ◽  
Amin Sedokani
Keyword(s):  
Helicobacter Pylori ◽  
Standard Treatment ◽  
Controlled Trial ◽  
Intervention Group ◽  
Trial Registration ◽  
Control Group ◽  
P Value ◽  
Bismuth Subcitrate ◽  
Significant Difference ◽  
H Pylori

Abstract Background: Considering the increase in drug resistance over time to Helicobacter pylori treatment relying on the anti-inflammatory and antibacterial effects of atorvastatin to increase the success rate of H. pylori eradication, we examined the effect of adding atorvastatin to standard treatment of H. pylori eradication.Results: A total of 186 symptomatic patients who had been diagnosed with Helicobacter pylori infection and tested for H. pylori eradication were examined by a pathological response or positive urea breath test. Patients who received atorvastatin in addition to standard treatment were also identified based on a table of random numbers. Standard treatment included a 240mg bismuth subcitrate tablet, a 40mg pantoprazole tablet, a 500mg metronidazole tablet, and 2 capsules of 500mg amoxicillin, all taken BID for 14 days. After 4 weeks of treatment, all patients underwent stool testing for H. pylori fecal antigen. If the test was positive, the request was considered a failure of treatment, and if the test was negative, it was considered a successful eradication of H. pylori. The clinical trial registration code for this study is IRCT20190823044589N1. The eradication rate of H. pylori was 80% in the control group and 80.9% in the intervention group, which did not show a statistically significant difference between the two groups (P-value=0.971).Conclusion: Adding atorvastatin to 4-drug regimen of PPI, bismuth subcitrate, amoxicillin, and metronidazole as the first line of treatment for H. pylori eradication is ineffective.Trial registration: IRCT, IRCT20190823044589N1. Registered 28 December 2019 - Retrospectively registered, https://en.irct.ir/trial/41734

Epidemiology Of Helicobacter Pylori In Delta Egypt

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S130-S130
Author(s):  
M Abdelmonem ◽  
M Elshamsy ◽  
H Wasim ◽  
M Shedid ◽  
A Boraik
Keyword(s):  
Helicobacter Pylori ◽  
Small Intestine ◽  
Standard Of Living ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gram Negative Bacteria ◽  
Stool Antigen ◽  
Males And Females ◽  
The Mean ◽  
H Pylori ◽  
Age Range

Abstract Introduction/Objective Introduction: Helicobacter pylori (H. pylori) infection is one the most prevalent bacterial infection in the world, affecting more than 50% of the world’s population. H. pylori are gram negative bacteria usually found in the stomach, penetrate the lining of the stomach, small intestine, or esophagus. After many years, they can cause sores, called ulcers, in the lining of your stomach or the upper part of your small intestine. For some people, an infection can lead to Gastric cancer which is the second most common cancer worldwide Objectives: The aim of this study is to predict the prevalence of Helicobacter pylori infection in gastrointestinal tract patients in Egypt. Methods Subjects and methods: A total of 1120 patients were enrolled in this study from The Delta region in Egypt. Enzyme-linked immunosorbent assay (ELISA) kits were used to detect H. pylori stool Antigen. Among the 1120 patient; 301 patients (26.9%) were males, 510 patients (45.6%) were females and 309 patients (27.5%) were children with age range from 1 years to 76 years. The mean of the age was 8,35,34 for children, males and females respectively. Results The overall prevalence of H. pylori infection was 52%. Among the 1120 patients, 576 patients (48%) were negative while 624 patients (52%) of the patients were Positive. It was observed that 169 patients (51.6%) of males were positive,275 patients (52.9%) of females were positive and 135 patients (41%) of children were positive. Furthermore, 133 patients (48.4%) of males were negative, 227 patients (47.2%) of females were negative and 181 patients 59% of children are negative. Conclusion This study revealed that the incidence and prevalence rates of H. Pylori in Egypt are relatively high. The high H. pylori prevalence is related to poor standard of living, low socio-economic conditions, poor sanitation and fecal contamination of food or water. It is crucial to launch educational awareness program for H. pylori in Egypt.

Serum Vitamin B12 and Folate Levels in Mothers and their Newborns: An Observational Study

2016 ◽  
Vol 50 (4) ◽  
pp. 190-193
Author(s):  
Priyanka H Krishnaswamy ◽  
Sujatha Prabhu ◽  
N Ashwini Pandith ◽  
Achal Shetty
Keyword(s):  
Observational Study ◽  
Vitamin B12 ◽  
Cord Blood ◽  
Serum Vitamin ◽  
P Value ◽  
Blood Concentrations ◽  
Large Numbers ◽  
B12 Deficiency ◽  
The Mean ◽  
Average Serum

ABSTRACT Aim To estimate the average serum values of vitamin B12 and folate in women with a term pregnancy and the cord blood of their newborns. To find if any correlation exists between the levels of serum B12 and folate in the mother and that of the neonate. Materials and methods A prospective observational study of serum B12 and folate levels and the respective cord blood concentrations of 51 term, nonanemic, pregnant women and in the cord blood of their newborns at delivery. Results Fifteen women were B12 deficient (29.4%) with the mean being 189.25 ± 94.2 ng/mL and 14 neonates were B12 deficient (27.45%) with their mean being 321.86 ± 143.68 ng/mL. One woman was folate deficient (1.96%) with the mean being 13.13 ± 5.15 ng/mL and one neonate was folate deficient (1.96%) with the mean being 15.68 ± 4.61 ng/mL. There was a significant correlation between maternal and neonatal B12 levels with a Pearson's coefficient of 0.74 (p-value of < 0.01) and a significant correlation between maternal and neonatal folate levels with a Pearson's coefficient of 0.44 (p-value of < 0.01). Conclusion There is a high incidence of maternal B12 deficiency in the Indian community, which has a positive correlation with neonatal levels. Treatment of B12 and folate deficiency should be a part of routine antenatal treatment, and neonates should be followed up and treated if found to be B12 or folate deficient. Clinical significance In India, where large numbers of women are anemic, deficiency of vitamin B12 and folate is overlooked when treatment of anemia is undertaken in most hospitals. In addition, women with undetected and subclinical deficiency may transmit it to their newborns with a cycle that is self-perpetuating unless broken with adequate intake or supplementation. How to cite this article Krishnaswamy PH, Prabhu S, Pandith NA, Shetty A. Serum Vitamin B12 and Folate Levels in Mothers and their Newborns: An Observational Study. J Postgrad Med Edu Res 2016;50(4):190-193.

scholarly journals Helicobacter pylori infection and vitamin B-12 deficiency- A cross sectional study

2017 ◽  
Vol 8 (4) ◽  
pp. 16-20 ◽  
Author(s):  
K. Ravi ◽  
Jacob Joseph ◽  
David Mathew Thomas
Keyword(s):  
Helicobacter Pylori ◽  
Vitamin B12 ◽  
Atrophic Gastritis ◽  
Histopathological Examination ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
Helicobacter Pylori Infection ◽  
Blood Levels ◽  
B12 Deficiency ◽  
H Pylori

Background: Vitamin B12 deficiency is a common, often overlooked medical problem in adult  population. Diagnosis of vitamin B12 deficiency is incomplete without the evaluation of underlying cause. In majority of the cases Vitamin B12 deficiency is attributed to malnutrition. H. pylori infection plays an important role in the development of atrophic gastritis and related malabsorption. Hence it is suggested that there may be a relationship between h. pylori infection and vitamin B12 deficiency.Aims and Objective: To evaluate correlation of helicobacter pylori infection and blood levels of vitamin B12.Materials and Methods: A total of 120 patients with deficient serum vitamin B12 levels were evaluated. Upper GI Endoscopy was performed and gastric biopsies were obtained for Histopathological examination and histological evidence of H. pylori infection.Results: Tissue biopsy revealed chronic atrophic gastritis in 65 patients and chronic antral gastritis in 39 patients. H. pylori infection by histology was positive in 68 patients. There was significant correlation between atrophic gastritis and H. pylori as well as between H. pylori and B12 deficiency.Conclusion: H.pylori has an effect on gastric mucosa, which influences the absorption of vitamin B12. Thus individuals with B12 deficiency must be subjected for diagnostic evaluation of H.pylori infection and appropriate therapy must be initiatedAsian Journal of Medical Sciences Vol.8(4) 2017 16-20

scholarly journals The Relation of Cytotoxin-Associated Gene-A Seropositivity with Vitamin B12 Deficiency in Helicobacter pylori-Positive Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-5
Author(s):  
Celal Ulasoglu ◽  
Hatice Esin Temiz ◽  
Zuhal Aydan Sağlam
Keyword(s):  
Helicobacter Pylori ◽  
Vitamin B12 ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
Inclusion Criteria ◽  
Lymphoid Follicles ◽  
Cytotoxin Associated Gene A ◽  
B12 Deficiency ◽  
Vitamin Level ◽  
H Pylori

Background and Aim. As a worldwide infectious bacterium, H. pylori leads to stomach pathologies such as gastritis, peptic ulcer, gastric cancer, MALToma, and various extragastric manifestations. In our study, we aimed to investigate the association between serum vitamin B12 level and cytotoxin-associated gene-A (CagA) seropositivity, which is one of the virulence factors of Helicobacter pylori (H. pylori). Method. This study has been conducted on 289 patients who have met the inclusion criteria. Within these patients, 213 of them were H. pylori positive and 76 were negative. Vitamin B12 and CagA-IgG levels were assessed in consecutive dyspeptic patients undergoing upper endoscopy. Results. Out of 289 patients, 51.9% were women (n = 150) and H. pylori was detected in 213 (73.7%) patients. Histopathological evaluation with modified Sydney classification revealed lymphocyte infiltration in 66.8% (n = 193), activation in 46% (n = 133), metaplasia in 11.4% (n = 33), atrophy in 11.4% (n = 33), and lymphoid follicles in 21.1% (n = 61) of the patients. Within H. pylori-positive patients, the ratio of CagA positivity was 57.3% (n = 122). Low B12 vitamin level was significantly correlated with existence of H. pylori (p=0.02), CagA (p=0.002), lymphocyte (p=0.006), metaplasia (p=0.001), atrophy (p=0.001), and lymphoid follicles (p=0.006). Positivity of CagA has been detected to be statistically corelated with lymphocyte (p=0.001) and activation (p=0.005); however, the same relation was not present with atrophy (p=0.236). Conclusion. In conclusion, B12 deficiency was positively correlated with CagA positivity and gastric inflammatory activity.

scholarly journals Prevalence of helicobacter pylori infection in children with Vitamin B12 Deficiency - A cross-sectional observational study

2021 ◽  
Vol 12 (7) ◽  
pp. 42-46
Author(s):  
Rajendra Kumar Dhayal ◽  
Shree Krishan Vishnoi ◽  
Rakesh Jora ◽  
Sandeep Choudhary
Keyword(s):  
Helicobacter Pylori ◽  
Vitamin B12 ◽  
Atrophic Gastritis ◽  
Histopathological Examination ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
Helicobacter Pylori Infection ◽  
Cross Sectional ◽  
B12 Deficiency ◽  
H Pylori

Background: Vitamin B12 deficiency is common in children but under diagnosed disorder. Helicobacter pylori infection plays an important role in the development of atrophic gastritis and related malabsorption. There may be a relationship between H.Pylori infection and vitamin B12 deficiency. Aims and Objective: To find out prevalence of Helicobacter pylori infection by endoscopic and histopathological findings, in children aged 2-18 years, with vitamin B12 deficiency. Materials and Methods: Seventy-eight patients with deficient serum vitamin B12 levels were evaluated. Upper GI Endoscopy was performed in all cases and gastric biopsies were obtained for histopathological examination and evidence of H. pylori infection. Results: Tissue biopsy revealed chronic atrophic gastritis in 09 patients and chronic antral gastritis in 52 patients. H. pylori infection by histology was positive in 45(57.70%) patients. We found significant correlation between atrophic gastritis and H. pylori infection, as well as between H. pylori infection and B12 deficiency. Conclusion: H.pylori has an effect on gastric mucosa, which affects the absorption of vitamin B12. Thus individuals with B12 deficiency should be subjected for diagnostic evaluation of H.pylori infection so that appropriate therapy can be initiated.

Helicobacter Pylori Infection in Sjögren’s Syndrome: Co-incidence or Causality?

2019 ◽  
Vol 15 (3) ◽  
pp. 238-241
Author(s):  
Massoud Saghafi ◽  
Nafiseh Abdolahi ◽  
Reza Orang ◽  
Mohammad Reza Hatef ◽  
Mohammad Hadi Molseghi
Keyword(s):  
Helicobacter Pylori ◽  
Serum Level ◽  
Sjögren’S Syndrome ◽  
Sjögren's Syndrome ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Control Group ◽  
P Value ◽  
H Pylori ◽  
Sjögren‘S Syndrome

Background and Aim: Lymphoid cell infiltration and destruction of exocrine glands, specifically lacrimal and salivary glands are characteristics of Sjogren’s syndrome (SS). An etiological role has been proposed for Helicobacter pylori (H. pylori), interacting in the clinical course and complications of SS (including gastric cancer and lymphoma). The aim of this study was to identify the probable correlation between H. pylori infection and Sjogren’s syndrome (SS). Methods: In this case-control study, ELISA method was used to determine serum level of IgA and IgM anti H. pylori antibody in 43 subjects with SS according to the international criteria and 95 healthy subjects as control. SPSS-17 was used to analyze data with t-test. P value <.05 were considered significant. Results: Serum level of IgM (34.9% vs. 10.5%, p-value= 0.001) and IgA (67.4% vs. 46.3% p value= 0.021) anti H. pylori antibody were significantly higher in SS patients compared to the control group. There was a positive correlation between age and H. pylori infection (r=0.2, Pvalue= 0.05). Conclusion: Patients with SS had a higher prevalence of H. pylori infection compared to the normal population. Eradication of H. pylori is recommended particularly in older patients with SS.

scholarly journals Helicobacter pylori eradication in patients with type 2 diabetes mellitus: Multicenter prospective observational study

2019 ◽  
Vol 7 ◽  
pp. 205031211983209 ◽  
Author(s):  
Seung-Joo Nam ◽  
Sung Chul Park ◽  
Sang Hoon Lee ◽  
Dong Wook Choi ◽  
Sung Joon Lee ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Observational Study ◽  
Prospective Observational Study ◽  
Eradication Rate ◽  
Laboratory Data ◽  
P Value ◽  
Diabetic Patients ◽  
Multicenter Prospective Observational Study ◽  
H Pylori

Objective: To compare Helicobacter pylori ( H. pylori) eradication rate of type 2 diabetic patients with non-diabetic subjects. Methods: In this multicenter prospective observational study, H. pylori-infected subjects were enrolled from three university-affiliated hospitals. Eradication regimen was triple therapy with standard dose of proton pump inhibitors (b.i.d), amoxicillin (1.0 g b.i.d), and clarithromycin (500 mg b.i.d) for 7 days. Urea breath test was performed 4 weeks after treatment. Various clinical and laboratory data were collected for identification of factors associated with successful eradication. Results: Totally, 144 subjects were enrolled and 119 (85 non-diabetic and 34 diabetic patients) were finally analyzed. Eradication rate was 75.6% and there was no difference between diabetic patients and non-diabetic subjects (73.5% vs 76.5%, p value: 0.814). Adverse drug reactions were reported in 44.5% of patients. In multivariate analysis for predicting H. pylori eradication in diabetic patients, HbA1c (⩾7.5%) was a significant factor affecting eradication rate (adjusted odds ratio: 0.100, 95% confidence interval: 0.011–0.909, p value: 0.041). Conclusion: Diabetes itself is not a major factor affecting H. pylori eradication. However, poor glucose control may harmfully affect H. pylori eradication.

scholarly journals A Novel Line Immunoassay Based on Recombinant Virulence Factors Enables Highly Specific and Sensitive Serologic Diagnosis of Helicobacter pylori Infection

2013 ◽  
Vol 20 (11) ◽  
pp. 1703-1710 ◽  
Author(s):  
Luca Formichella ◽  
Laura Romberg ◽  
Christian Bolz ◽  
Michael Vieth ◽  
Michael Geppert ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Immune Responses ◽  
Virulence Factors ◽  
Gastrointestinal Disease ◽  
Individual Risk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Serologic Test ◽  
Content Type ◽  
H Pylori

ABSTRACTHelicobacter pyloricolonizes half of the world's population, and infection can lead to ulcers, gastric cancer, and mucosa-associated lymphoid tissue (MALT) lymphoma. Serology is the only test applicable for large-scale, population-based screening, but current tests are hampered by a lack of sensitivity and/or specificity. Also, no serologic test allows the differentiation of type I and type II strains, which is important for predicting the clinical outcome.H. pylorivirulence factors have been associated with disease, but direct assessment of virulence factors requires invasive methods to obtain gastric biopsy specimens. Our work aimed at the development of a highly sensitive and specific, noninvasive serologic test to detect immune responses to importantH. pylorivirulence factors. This line immunoassay system (recomLine) is based on recombinant proteins. For this assay, six highly immunogenic virulence factors (CagA, VacA, GroEL, gGT, HcpC, and UreA) were expressed inEscherichia coli, purified, and immobilized to nitrocellulose membranes to detect serological immune responses in patient's sera. For the validation of the line assay, a cohort of 500 patients was screened, of which 290 (58.0%) wereH. pylorinegative and 210 (42.0%) were positive by histology. The assay showed sensitivity and specificity of 97.6% and 96.2%, respectively, compared to histology. In direct comparison to lysate blotting and enzyme-linked immunosorbent assay (ELISA), therecomLine assay had increased discriminatory power. For the assessment of individual risk for gastrointestinal disease, the test must be validated in a larger and defined patient cohort. Taking the data together, therecomLine assay provides a valuable tool for the diagnosis ofH. pyloriinfection.

scholarly journals Histopathological Changes in Helicobacter pylori Associated Gastritis and Scope of Special Stain and Immunohistochemistry as Diagnostic Aids

2020 ◽  
Vol 7 (50) ◽  
pp. 3027-3032
Author(s):  
Ruby Elizabeth Elias ◽  
Bindiya Gisuthan ◽  
Sreeganesh A.S
Keyword(s):  
Helicobacter Pylori ◽  
Positive Predictive Value ◽  
Negative Predictive Value ◽  
Chronic Gastritis ◽  
Predictive Value ◽  
Diagnostic Methods ◽  
P Value ◽  
Special Stain ◽  
Frequent Finding ◽  
H Pylori

BACKGROUND Helicobacter pylori associated chronic gastritis plays a vital role in the development of majority of gastric adenocarcinomas and most gastric MALT (Mucosa Associated Lymphoid Tissue) lymphomas. Many diagnostic methods are available for the identification of this organism. However, in gastroenterology practice, histopathological examination of biopsy samples provides visual identification of the pathogen and the associated mucosal changes with special stains like Giemsa. The aim of this study was to evaluate the efficacy of three stains H & E- (Haematoxylin and Eosin), Giemsa and IHC (Immunohistochemistry) in the identification of H. pylori. Associated histologic changes were noted and the relationship between the degree of colonisation and the activity and chronicity of gastritis were analysed. METHODS 585 gastric biopsies taken from dyspeptic patients were evaluated for gastritis, based on updated Sydney System. In 250 randomly selected cases, three staining methods were used. RESULTS Out of 585 cases, 413 (70.60 %) had features of chronic gastritis. Mild chronic gastritis was the commonest finding and is seen in most cases of mild H. pylori colonisation. When activity was monitored, mild activity was the most frequent finding [225 (38.46 %)]. Majority of the severe activity cases showed severe H. pylori colonisation. 13.16 %, 4.79 % and 7.35 % showed intestinal metaplasia, atrophy and dysplastic changes respectively. Out of 250 cases, H & E and Giemsa stains showed 45.6 % and 57.2 % positivity while IHC demonstrated maximum number of positivity (156 cases - 62.4 %). Sensitivity and specificity of H & E was found to be 77.90 % and 98.95 %, positive predictive value was 99.13 % and negative predictive value was 69.18 %. For Giemsa stain, sensitivity was 91.67 %, specificity was 100 %, positive predictive value was 100 % and negative predictive value was 87.85 %. DISCUSSION H. pylori gastritis was a frequent finding in dyspeptic patients in southern part of India. When chi-square test was done, a significant statistical relationship between the severity of H. pylori colonisation, activity and chronicity of gastritis was noted. P value was < 0.001. With the use of special stain, Giemsa and ancillary techniques like IHC, the detection rate of H. pylori was enhanced considerably. CONCLUSIONS With increasing number of H. pylori in the mucosa, there was increase in the chronicity and activity of gastritis. Although immunohistochemistry revealed more cases of H. pylori, Giemsa can be a cost-effective substitute, because of its high specificity and positive predictive value. KEYWORDS H. pylori Gastritis, Giemsa, Haematoxylin and Eosin Stain, Immunohistochemistry

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