A Semiautomated Method for Determining Amylase Activity in Serum and Urine

1970 ◽  
Vol 16 (5) ◽  
pp. 390-395 ◽  
Author(s):  
John A Lott ◽  
Joan E Mercier
Keyword(s):  
Reducing Sugars ◽  
Normal Values ◽  
Amylase Activity ◽  
Potassium Ferricyanide ◽  
Optimum Conditions ◽  
Endogenous Glucose ◽  
Semiautomated Method ◽  
Serum And Urine

Abstract Serum or urine is incubated at 50°C with a substrate containing dextrin. The endogenous glucose and reducing sugars liberated from the substrate by amylase are separately determined on the AutoAnalyzer by use of alkaline potassium ferricyanide. Optimum conditions and limitations were evaluated. The method is compared to those of Henry and Chiamori (Somogyi) and Fishman and Doubilet. Normal values for serum and urine are presented.

Amylase activity in serum and urine: comparison of results by the amyloclastic, dyed-starch, and nephelometric techniques.

1977 ◽  
Vol 23 (6) ◽  
pp. 1061-1065 ◽  
Author(s):  
D P Lehane ◽  
P J Wissert ◽  
G Lum ◽  
A L Levy
Keyword(s):  
Normal Values ◽  
Amylase Activity ◽  
Rank Correlation ◽  
Correlation Coefficients ◽  
Spearman Rank Correlation ◽  
Upper Limit ◽  
Kolmogorov Smirnov ◽  
Urine Specimens ◽  
Smirnov Statistic ◽  
Serum And Urine

Abstract We assayed serum and urine specimens for amylase activity by the nephelometric (I),dyed-starch (Amylochrome) (II), and mayloclastic (III) techniques. For serum, the correlation coefficients of the regression lines were: I vs. II, 0.978 (n = 106); I vs. III, 0.736 (n = 110); and II vs. III, 0.739 (n = 108). For urine, they were I vs. II, 0.938 (n = 49); and I vs. III, 0.752 (n = 46). Because calculation of the Kolmogorov-Smirnov statistic showed the distributions to be nongaussian, Spearman rank correlation coefficients were determined and showed that I and II correlated well but neither method correlated with III. The clinical data show that I and II gave above-normal activities in every case of pancreatitis, but III gave normal values in two of eight cases. In all cases, I and II were more sensitive, giving higher amylase activities (as compared with the upper limit of normal) than did III. The nephelometric procedure is most suitable for routine and emergency testing; the dyed-starch assay is equally sensitive and reliable, but less convenient. The amyloclastic procedure appears to be less reliable.

New saccharogenic determination of alpha-amylase in serum and urine.

1979 ◽  
Vol 25 (2) ◽  
pp. 215-217 ◽  
Author(s):  
L van Leeuwen
Keyword(s):  
Human Serum ◽  
Amylase Activity ◽  
Glucose Dehydrogenase ◽  
Alpha Amylase ◽  
Endogenous Glucose ◽  
Alpha Glucosidase ◽  
Serum And Urine

Abstract I describe a new kinetic enzymatic saccharogenic method for assaying alpha-amylase in human serum and urine. alpha-Amylase liberates maltose from starch. This is successively acted on by alpha-glucosidase, mutarotase, and glucose dehydrogenase. The resulting conversion of NAD+ to NADH, measured at 340 nm, during a 20-min incubation reflects amylase activity. Endogenous glucose is destroyed before measurement of amylase activity is begun.

N-Acetyl-β-D-Glucosaminidase Enzymuria in Leukaemia and Myelomatosis: Effect of Treatment in Acute Myeloblastic Leukaemia and Myelomatosis in Adults

1986 ◽  
Vol 23 (6) ◽  
pp. 676-680 ◽  
Author(s):  
P H Whiting ◽  
D J King ◽  
A Ireland ◽  
M A Ratcliffe ◽  
A A Dawson
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Lymphocytic Leukaemia ◽  
Normal Values ◽  
Terminal Phase ◽  
Acute Myeloblastic Leukaemia ◽  
Lysosomal Hydrolase ◽  
Patient Groups ◽  
Effect Of Treatment ◽  
Pre Treatment ◽  
Serum And Urine

The activity of the lysosomal hydrolase N-acetyl-β-d-glucosaminidase (NAG) was measured in the urine of patients with leukaemia or myeloma. Elevated pre-treatment enzymuria was noted in all patient groups with acute myeloblastic leukaemias (AML) FAB type M4 or 5 displaying higher activities than AML patients FAB types M1–3, which in turn were higher than those found in patients with myelomatosis and chronic lymphocytic leukaemia. The ratio of the major isoenzymes of NAG, A/B was reduced significantly only in patients with AML. Following treatment, AML patients who entered remission demonstrated NAG levels which approached normal values. In those AML patients who were either in relapse, in the terminal phase of their illness or treated with aminoglycoside antibiotics, NAG enzymuria was similar to pre-treatment values. A reduction in urinary NAG levels and both serum and urine β2 microglobulin concentrations was also observed following treatment in myeloma patients. The use of enzymuria both as a guide to progress towards remission in AML patients and for assessing prognosis and progress in myeloma patients is discussed.

Hydroxyproline in the Serum and Urine Normal Values and Clinical Significance

2009 ◽  
Vol 179 (3) ◽  
pp. 275-284 ◽  
Author(s):  
O. LAITINEN ◽  
E. A. NIKKILÄ ◽  
K. I. KIVIRIKKO
Keyword(s):  
Clinical Significance ◽  
Normal Values ◽  
Serum And Urine

Enzyme-coupled ultraviolet determination of alpha-amylase activity with the GEMSAEC centrifugal analyzer.

1978 ◽  
Vol 24 (9) ◽  
pp. 1620-1624 ◽  
Author(s):  
W H Porter ◽  
R E Roberts
Keyword(s):  
Linear Response ◽  
Amylase Activity ◽  
Kinetic Studies ◽  
Alpha Amylase ◽  
Coefficients Of Variation ◽  
Endogenous Glucose ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Alpha Glucosidase ◽  
As Effects

Abstract We evaluated the Harleco alpha-glucosidase/hexokinase/glucose-6-phosphate dehydrogenase-coupled alpha-amylase method, bu use of the GEMSAEC centrifugal analyzer. Performance evaluation included kinetic studies of substrate and maltose hydrolysis as well as effects of endogenous glucose and fructose. The reagent was found to give a linear response with alpha-amylase activity to greater than 1200 U/liter. Within-run precision resulted in coefficients of variation (CV) of 0.9 to 3.2% over the range studied. Day-to-day precision corresponded to CV's of 2.4 to 4.4% over the same range of alpha-amylase procedure was found to be good (r = 0.997) for patients' sera examined.

scholarly journals Changes in amylase activity starch and sugars contents in mango fruits pulp cv. Tommy Atkins with spongy tissue

2001 ◽  
Vol 44 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Luiz Carlos de Oliveira Lima ◽  
Adimilson Bosco Chitarra ◽  
Maria Isabel F. Chitarra
Keyword(s):  
Adverse Effects ◽  
Carbohydrate Metabolism ◽  
Enzyme Activities ◽  
Reducing Sugars ◽  
Amylase Activity ◽  
Starch Content ◽  
Mangifera Indica ◽  
Spongy Tissue ◽  
Mango Fruit ◽  
Appreciable Increase

Changes in amylase activity, starch and reducing and non-reducing sugars contents were monitored during ripening of mango fruits (Mangifera indica L.). The climateric raising in mango fruit is marked by an appreciable increase in the activity of amylase, reducing and non-reducing sugars contents and decrease in the starch content. The fruit affected with spongy tissue exhibited much lower amylase activity and reducing and non-reducing sugars, but exhibited much higher starch content during storage at 12 ± 2° C and 90 ± 5% RH for 28 days, when compared to healthy tissue of ‘Tommy Atkins’. Whether this is caused due to adverse effects on certain enzyme activities during ripening is not clearly known. These dates showed that carbohydrate metabolism is an important feature during ripening of mango.

Isopropylidine maltoheptosyl fructofuranoside, doubly blocked substrate for determination of endoamylase activity

1991 ◽  
Vol 37 (8) ◽  
pp. 1323-1328
Author(s):  
Z Ogawa ◽  
Y Matsubayashi ◽  
S Satoh ◽  
N Orita ◽  
H Itoh
Keyword(s):  
Human Serum ◽  
Coefficient Of Variation ◽  
Oxidation Rate ◽  
Amylase Activity ◽  
Mannitol Dehydrogenase ◽  
Coupled Reactions ◽  
Alpha Glucosidase ◽  
Serum And Urine

Abstract We synthesized o-(4,6-o-isopropylidene-alpha-D-glucopyranosyl)-(1----4)- [o-alpha-D-glucopyranosyl-(1----4])5-o-alpha-D-glucopyranosyl-(1----2)- alpha-D-fructofuranoside (IPG7F) and developed an assay for determining the activity of amylase in human serum and urine by using this substrate. Glucoamylase, alpha-glucosidase, and mannitol dehydrogenase are used as coupling enzymes. The coupled reactions are monitored by continuously measuring the oxidation rate of NADH. In this procedure, various substances in the test specimens do not interfere with the detection of amylase activity. Exactly one molecule of NADH is oxidized by one attack of amylase on the substrate, although four products can be produced in the reaction. The within-assay coefficient of variation (CV) ranged from 1.0% to 4.1% and the between-assay CV ranged from 2.6% to 5.3%. The results of our new assay correlate well with those of the amylase assay involving p-nitrophenol maltoheptaoside as substrate (r = 0.978) and with those of the amylase assay involving maltopentaose (r = 0.987).

scholarly journals Inhibition of Serum and Urine Amylase Activity in Pancreatitis with Hyperlipemia

1975 ◽  
Vol 182 (1) ◽  
pp. 72-75 ◽  
Author(s):  
ANDREW L. WARSHAW ◽  
CYNTHIA A. BELLINI ◽  
PAUL B. LESSER
Keyword(s):  
Amylase Activity ◽  
Urine Amylase ◽  
Serum And Urine

Nutrient homeostasis, metabolism of reserves, and seedling vigor as affected by seed priming in coarse rice

2006 ◽  
Vol 84 (8) ◽  
pp. 1196-1202 ◽  
Author(s):  
M. Farooq ◽  
S.M.A. Basra ◽  
M. Khalid ◽  
R. Tabassum ◽  
T. Mahmood
Keyword(s):  
Reducing Sugars ◽  
Amylase Activity ◽  
Nitrogen Concentration ◽  
Ion Homeostasis ◽  
Seed Priming ◽  
Dry Mass ◽  
Seedling Vigor ◽  
Control Seed ◽  
Positive Correlations ◽  
Secondary Roots

The influence of seed priming on germination, seedling vigor, ion homeostasis, and starch metabolism in coarse rice was studied. Priming treatments included pregermination (a traditional soaking method being used for rice nursery preparation), hydropriming for 48 h, osmohardening with KCl or CaCl2 (ψs –1.25 MPa solution) for 24 h, ascorbate priming (10 mg·L–1) for 48 h, and hardening for 24 h. Compared with controls, all priming treatments (except pregermination) reduced the time to start germination, improved the rate of germination and synchronization, and the length of shoot and root, seedling fresh and dry mass, number of secondary roots, the concentration of reducing sugars, and α-amylase activity, although the extent of these changes was different in seeds subjected to different treatments. These seed treatments resulted in higher germination that might be due to overcoming dormancy. Osmohardening with KCl was more effective than CaCl2 for these parameters. Nitrogen concentration remained unaffected in seedlings; however, Ca2+ concentrations in both seeds and seedlings were greater in seeds osmohardenerd with CaCl2 than with all other treatments, including the control. Seed priming enhanced K+ concentration in both seeds and seedlings, leading to improved α-amylase activity. There were positive correlations between seed K+ concentration and amylase activity, and the concentration of reducing sugars with amylase activity, seedling dry mass, or number of secondary roots. Osmohardening with KCl performed better than all other treatments including control. Priming improved the K+ balance that activates α-amylase, a basis for seed invigoration.

Sign in / Sign up

Export Citation Format

Share Document