Simplified Colorimetric Determination of Thiocyanate in Biological Fluids, and Its Application to Investigation of the Toxic Amblyopias

1972 ◽  
Vol 18 (9) ◽  
pp. 996-1000 ◽  
Author(s):  
A R Pettigrew ◽  
G S Fell
Keyword(s):  
Cigarette Smoking ◽  
Analytical Procedure ◽  
Biological Fluids ◽  
Colorimetric Determination ◽  
Subsequent Increase ◽  
Cyanide Toxicity ◽  
Colorimetric Procedure ◽  
Colorimetric Reaction ◽  
Hereditary Optic Atrophy

Abstract A colorimetric procedure for determination of small amounts of cyanide and thiocyanate, involving the synthesis of a pyridine dyestuff by the reaction of pyridine and an aromatic amine, has been simplified for the estimation of thiocyanate alone in biological fluids. Replacement of benzidine with p-phenylenediamine in the colorimetric reaction has both improved the precision of the analytical procedure and avoided a carcinogenic hazard. This method has been used to follow the decrease in plasma thiocyanate associated with abstinence from cigarette smoking, and its subsequent increase upon resumption. It has also been used to measure the plasma and urinary thiocyanate concentrations of patients suffering from the particular toxic amblyopias—tobacco amblyopia and Leber’s hereditary optic atrophy— believed to be associated with cyanide toxicity, and to follow the increased thiocyanate concentrations that accompany significant improvements in the patients’ vision brought about by various treatments.

scholarly journals Derivative Spectrophotometric Analysis of 4-Quinolone Antibacterials in Formulations and Spiked Biological Fluids by Their Cu(II) Complexes

2001 ◽  
Vol 84 (2) ◽  
pp. 368-375 ◽  
Author(s):  
Mohammed Rizk ◽  
Fathalla Belal ◽  
Fawiza Ibrahim ◽  
Soad Ahmed ◽  
Zeinab A Sheribah
Keyword(s):  
Aqueous Medium ◽  
Linear Correlation ◽  
Analytical Procedure ◽  
Biological Fluids ◽  
Reference Method ◽  
Spectrophotometric Analysis ◽  
Third Order ◽  
Selective Determination ◽  
Average Percentage

Abstract A derivative UV-spectrophotometric analytical procedure was developed for determination of three 4-quinolone antibacterials: norfloxacin (NFX), ciprofloxacin (CFX), and sparfloxacin (SFX). The method depends on the complexation of Cu(II) with the studied compounds in aqueous medium. A third order, measurement was applied for their quantification. A linear correlation was established between the amplitude of the peak and concentration for all the studied drugs in the range of 15–80, 35–120, and 200–700 ng/mL, with minimum detectability (S/N = 2) of 1.0, 1.3, and 5.1 ng/mL for NFX, CFX, and SFX, respectively. The method was successfully applied for accurate, sensitive, and selective determination of the studied drugs in bulk and tablets formulation with average percentage recoveries of 99.22 ± 0.55 to 100.33 ± 1.60. The results obtained were favorably compared with those of the reference method. The method was also used to determine sparfloxacin in spiked human plasma and urine. The results obtained were satisfactory, accurate, and precise.

Colorimetric Determination of Procaine Penicillin in Mixed Feeds

1965 ◽  
Vol 48 (4) ◽  
pp. 702-704
Author(s):  
Loyal R Stone
Keyword(s):  
Chemotherapeutic Agents ◽  
Colorimetric Determination ◽  
Procaine Penicillin ◽  
Acid Reagent ◽  
Mixed Feeds ◽  
Colorimetric Procedure

Abstract Procaine penicillin is determined by a rapid colorimetric procedure based on conversion of penicillin to penicillamine which turns blue with arsenomolybdic acid reagent. The presence of other antibiotics and chemotherapeutic agents in the feed does not interfere. The method is suitable over the range 10–100 g procaine penicillin per ton of feed.

Determination of Orotic and Dihydroorotic Acids in Biological Fluids and Tissues

1975 ◽  
Vol 21 (3) ◽  
pp. 353-355 ◽  
Author(s):  
Leo Kesner ◽  
Frank L Aronson ◽  
Morris Silverman ◽  
Phillip C Chan
Keyword(s):  
Organic Acid ◽  
Orotic Acid ◽  
Colorimetric Assay ◽  
Biological Fluids ◽  
Acid Fraction ◽  
Chromatographic Procedure ◽  
Colorimetric Procedure ◽  
Column Chromatographic ◽  
Total Organic Acid

Abstract In commonly used procedures for colorimetric assay of orotic acid in biological materials, certain substances interfere, positively or negatively. Some that interfere negatively are ornithine, cysteine, citrulline, tyrosine, proline, hydroxyproline, and polyamines. Protein and interfering agents are easily removed by first isolating the total organic acid fraction. The orotic acid in this fraction can then be determined by an established colorimetric procedure. A rapid liquid—liquid column-chromatographic procedure is described, if analysis of both dihydroorotic and orotic acid is required.

Colorimetric Determination of Blood Arginase

1955 ◽  
Vol 1 (2) ◽  
pp. 110-116 ◽  
Author(s):  
Max M Friedman ◽  
Edythe Becker
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Colorimetric Determination ◽  
Normal Human ◽  
Colorimetric Procedure

Abstract A colorimetric procedure for blood arginase has been described, based on hemolyzing the red blood cells with saponin and determining the urea formed by action of the enzyme on an arginine substrate. The range of blood arginase in a series of normal human subjects has been presented.

Semiautomated Colorimetric Determination of Methenamine and Methenamine Mandelate in Tablets

1973 ◽  
Vol 56 (3) ◽  
pp. 647-652 ◽  
Author(s):  
Matthew L Dow
Keyword(s):  
Chromotropic Acid ◽  
Assay Method ◽  
Colorimetric Determination ◽  
Coefficients Of Variation ◽  
Colorimetric Procedure

Abstract A semiautomated chromotropic acid colorimetric procedure was collaboratively studied by 6 laboratories, both as a composite assay and as an individual tablet assay method. Results agreed well with the USP and NF methods. The coefficients of variation ranged from 0.54 to 2.48%. The method has been adopted as official first action for the analysis of coated and uncoated tablets of methenamine mandelate, methenamine, and methenamine with sodium biphosphate.

Automated Colorimetric Determination of Phenylephrine Hydrochloride in Drug Formulations. I. Development of the Method

1971 ◽  
Vol 54 (3) ◽  
pp. 596-599
Author(s):  
Jonathan R Lane
Keyword(s):  
Flow Dynamics ◽  
Colorimetric Determination ◽  
Good Resolution ◽  
Reaction Conditions ◽  
Flow Lines ◽  
Phenylephrine Hydrochloride ◽  
Relative Standard ◽  
Solution Preparation ◽  
Colorimetric Reaction

Abstract The Emerson colorimetric reaction as modified by Koshy and Mitchner was adapted for automation of the phenylephrine assay. Using the AutoAnalyzer, studies were made of different reagent concentrations and reagent volume ratios to satisfy the reaction conditions of the official final action method, 36.112-36.114. The debubbler-flowcell design was changed to improve the flow dynamics, and the internal volumes of all unsegmented flow lines were minimized to permit good resolution at fast sampling ratios (50/hr). Relative standard deviations of less than 0.5% were obtained on multiple determinations of the same solutions. Essentially 100% recovery was obtained on assays of phenylephrine HCl added to a composite nasal solution preparation.

Development of test placers for blister colorimetric analysis of phosphate ions

2020 ◽  
pp. 24-29
Author(s):  
Aleksey Zhevnerov ◽  
Ravil Baybyekov ◽  
Sergey Belopuhov ◽  
Andrey Bochkarev
Keyword(s):  
Quantitative Determination ◽  
Phosphate Concentration ◽  
Colorimetric Determination ◽  
Colorimetric Analysis ◽  
Ion Concentrations ◽  
Phosphate Ion ◽  
Phosphate Ions ◽  
Preliminary Preparation ◽  
Colorimetric Reaction

Variants of the compositions of a series of placers for visually blister colorimetric determination of phosphate ion concentrations in a blister cell (tablet for tablets) containing a mixture of reagents are proposed. The placers allow quantitative determination of the phosphate concentration in various objects without preliminary preparation of reagent solutions. The application of reagents to the surface of silica gel makes it possible to isolate all reagents until a chemical reaction occurs. The composition and conditions of deposition of crystalline substances – reagents capable of providing a sufficiently high acidity with the addition of a few drops of a solution and an optimal reducing agent for blister conditions were optimized. The visual colorimetric reaction is carried out under conditions when the volume of the added solution is 0.1–0.2 ml.

A time-insensitive colorimetric sensor for the determination of total protein

RSC Advances ◽  
2016 ◽  
Vol 6 (57) ◽  
pp. 52026-52033
Author(s):  
Bahram Hemmateenejad ◽  
Arezoo Shahrivar-kevishahi ◽  
Fatemeh Shakerizadeh-Shirazi ◽  
Shohre Rouhani ◽  
Fereshteh Mohamadi-Gharaghani
Keyword(s):  
Total Protein ◽  
Biological Fluids ◽  
Colorimetric Sensor ◽  
Cyanine Dye ◽  
Colorimetric Determination

A newly synthesized cyanine dye was used for sensitive colorimetric determination of total protein in biological fluids.

Colorimetric Determination of Ethambutol Hydrochloride

1982 ◽  
Vol 65 (4) ◽  
pp. 899-900
Author(s):  
Dattatray M Shingbal ◽  
Shailendra D Naik
Keyword(s):  
Drug Concentration ◽  
Colorimetric Determination ◽  
Analytical Control ◽  
The Relationship ◽  
Colorimetric Procedure

Abstract A precise colorimetric procedure is proposed for estimation of ethambutol hydrochloride, based on reaction of the drug with 2,4-dinitro-l-fluorobenzene under stipulated conditions. The yellow chromophore is quantitated spectrophotometrically at 376 ± 1 nm. The relationship between absorbance and drug concentration was linear within a range of 5-40 μg/mL. The method is suitable for the analytical control of ethambutol HCl and its formulations.

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