Enzymatic Blood Glucose Determination by Colorimetry of N,N-Diethylaniline-4-Aminoantipyrine

1974 ◽  
Vol 20 (5) ◽  
pp. 606-607 ◽  
Author(s):  
Peter Kabasakalian ◽  
Sami Kalliney ◽  
Anita Westcott
Keyword(s):  
Hydrogen Peroxide ◽  
Blood Glucose ◽  
Glucose Oxidase ◽  
Oxidative Coupling ◽  
Room Temperature ◽  
Glucose Determination ◽  
Final Volume ◽  
Blood Glucose Determination

Abstract The hydrogen peroxide produced from glucose by glucose oxidase action at pH 7 is determined by the peroxidase oxidative coupling of N,N-diethylaniline with 4-amino-antipyrine. A 20-µl sample of plasma or serum is reacted at room temperature for 10 min. The sensitivity of the method is such that 60 µg of glucose (300 mg/dl) in a final volume of 5 ml gives an absorbance of 0.8 at 553 nm with a 1-cm cell. Absorbances are linearly related to glucose concentrations as high as 10.00 g/liter.

scholarly journals Blood glucose determination with an acetyl resorufin-glucose oxidase system as a fluorometric indicator reaction.

2001 ◽  
Vol 50 (7) ◽  
pp. 475-479 ◽  
Author(s):  
Shinya MATSU-URA ◽  
Yuji YAMAUCHI ◽  
Hidenobu OHMORI ◽  
Hatsuo MAEDA
Keyword(s):  
Blood Glucose ◽  
Glucose Oxidase ◽  
Oxidase System ◽  
Glucose Determination ◽  
Indicator Reaction ◽  
Blood Glucose Determination

Glucose Oxidase Method for Continuous Automated Blood Glucose Determination

1969 ◽  
Vol 15 (8) ◽  
pp. 680-698 ◽  
Author(s):  
John W Rosevear ◽  
Kenneth J Pfaff ◽  
Frederick J Service ◽  
George D Molnar ◽  
Eugene Ackerman
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Blood Glucose ◽  
Glucose Oxidase ◽  
Normal Subjects ◽  
Significant Inhibition ◽  
Total Response ◽  
Glucose Determination ◽  
Blood Glucose Determination ◽  
Sampling Catheter

Abstract A glucose oxidase-peroxidase method for continuous automated monitoring of blood glucose has been developed. The response is linear over the range 0-800 mg/100 ml. Sensitivity can be maintained for 24 hr or longer and can be restored by rinsing the analytic system with sulfuric acid to permit studies of longer than 48 hr in duration. A precision of ± 1% can be maintained between rinses for samples containing 100-600 mg of glucose per 100 ml. This method is satisfactorily specific for glucose: The response with other sugars is less than 1% of the response obtained with the same concentration of glucose. Ascorbic acid causes no significant inhibition of the response to glucose. The inhibition by uric acid has been reduced fifty-fold compared to that in other methods. Transit through the sampling catheter and analytic system requires 15 min. Timed from the first detectable response to a change in concentration, 25% of total response is achieved in 30 sec and 90% in 80 sec. Fifty percent of an oscillation with a half-period of 45 sec can be detected; no oscillations this short were observed in records of human blood glucose. Applicability and feasibility of this method have been demonstrated in over 2000 hr of repeated blood glucose recordings in 12 diabetic and 6 normal subjects.

Whole blood glucose determination using glucose oxidase immobilized on cotton cheese cloth

1997 ◽  
Vol 338 (1-2) ◽  
pp. 135-140 ◽  
Author(s):  
Sangita D. Kumar ◽  
A.V. Kulkarni ◽  
R. Kalyanraman ◽  
T.S. Krishnamoorthy
Keyword(s):  
Blood Glucose ◽  
Glucose Oxidase ◽  
Whole Blood ◽  
Glucose Determination ◽  
Blood Glucose Determination ◽  
Cheese Cloth

Interference by acetaminophen in the glucose oxidase-peroxidase method for blood glucose determination.

1976 ◽  
Vol 22 (10) ◽  
pp. 1729-1731 ◽  
Author(s):  
I Kaufmann-Raab ◽  
H G Jonen ◽  
E Jähnchen ◽  
G F Kahl ◽  
U Groth
Keyword(s):  
Blood Glucose ◽  
Glucose Oxidase ◽  
Analytical Procedure ◽  
Phenolic Hydroxyl ◽  
Hydroxyl Group ◽  
Glucose Determination ◽  
Apparent Loss ◽  
Blood Glucose Determination ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Related Compounds

Abstract Acetaminophen, p-aminophenol, and oxyphenbutazone interfere with the glucose oxidase/peroxidase method for glucose. Structurally related compounds that lack a free phenolic hydroxyl group (acetanilide, aniline, and phenylbutazone) do not interfere. During the analytical procedure acetaminophen is consumed. One mole of acetaminophen leads to an apparent loss of four moles of glucose. The hexokinase/glucose-6-phosphate dehydrogenase method (Boehringer Hexokinase method) is not affected by these substances.

scholarly journals Errata

1969 ◽  
Vol 15 (12) ◽  
pp. 1248-1248
Keyword(s):  
Blood Glucose ◽  
Glucose Oxidase ◽  
Glucose Determination ◽  
True Value ◽  
Blood Glucose Determination

Abstract In Rosevear, J. W. et al: "Glucose Oxidase Method for Continuous Automated Blood Glucose Determination, 15:680, 1969 in the Journal, the bottom of the whole paragraph on page 681 should have read: For a range of 0-500 mg/100 ml, the error equivalent to ±0.2% transmittance would be ±5% of the true value at 50 mg/100 ml, ±2% at 100, ±1% at 200, and ±3% at 500 mg/100 ml. For a range of 0-250 mg/100 ml (30-100% transmittance), the error equivalent to ±0.2% transmittance would be at least ±2% at 50 mg/100 ml and ±1% at 100 mg/100 ml.

Progress of MIR Non-Invasive Blood Glucose Determination and Effect of Stratum Corneum

2011 ◽  
Vol 31 (9) ◽  
pp. 0900105
Author(s):  
陈星旦 Chen Xingdan ◽  
王动民 Wang Dongmin ◽  
卢启鹏 Lu Qipeng ◽  
丁海泉 Ding Haiquan
Keyword(s):  
Blood Glucose ◽  
Stratum Corneum ◽  
Glucose Determination ◽  
Non Invasive ◽  
Blood Glucose Determination

Effect of Addition of WZB117 as an Inhibitor of Glucose Transporter 1 for Venous Blood Glucose Determination

2020 ◽  
Author(s):  
Lei Zhang ◽  
Yaqiong Ran ◽  
Yan Zhu ◽  
Qianna Zhen
Keyword(s):  
Blood Glucose ◽  
Glucose Level ◽  
Glucose Transporter ◽  
Venous Blood ◽  
Sugar Transport ◽  
Glucose Transporter 1 ◽  
Glucose Determination ◽  
Glucose Levels ◽  
Significant Difference ◽  
Blood Glucose Determination

Abstract Objective Sodium fluoride (NaF) has been applied to inhibit glycolysis in venous specimens for decades. However, it has had little effect on the rate of glycolysis in the first 1 to 2 hours, resulting in a decrease of glucose, so a more efficient method is needed. Recently, we discovered that WZB117, a specific Glut1 inhibitor, restricts glycolysis by inhibiting the passive sugar transport of human red blood cells and cancer cells. The purpose of this study was to evaluate the results of intravenous blood glucose determination after the addition of WZB117. Methods Venous specimens from 40 pairs of healthy volunteers were collected for several days and placed in tubes containing NaF plus EDTA-disodium (Na2) without WZB117 (the A group); citric acid, trisodium citrate, and EDTA-Na2 without WZB117 (B group); and NaF plus EDTA-Na2 with WZB117 (C group). The glucose concentration was measured after venipuncture and compared with test tubes treated for 1 hour, 2 hours, and 3 hours before centrifugation. Glucose level was determined by the hexokinase method. The paired t-test was used to examine differences in glucose values at baseline and at different time points. The number of misdiagnoses and the misdiagnosis rate were calculated at 2 diagnostic stages: high risk of diabetes (glucose level of 6.1 mmol/L) and diagnosis of diabetes (glucose level of 7.0 mmol/L). Results Glucose levels decreased by 1.0% at 1 hour and by 2.1% at 3 hours in the C group tubes and simultaneously decreased by 1.7% at 1 hour and by 2.5% at 3 hours in the B group tubes. In contrast, glucose levels decreased by 4.1% at 1 hour and by 6.3% at 3 hours in the A group tubes. There was a statistically significant difference in glucose levels measured in the A group tubes and B group tubes at 1 hour, 2 hours, and 3 hours. The misdiagnosis rate of clinical diagnosis in diabetes was highest in the A group tubes (7.0‰ at 1 hour, 0.1‰ at 3 hours at 7.0 mmol/L point; 14.6‰ at 1 hour, 0.4‰ at 3 hours at 6.1 mmol/L point) and lowest in the C group tubes (2.95‰ at 1 hour, 0‰ at 3 hours at 7.0 mmol/L point; 4.8‰ at 1 hour, 0.1‰ at 3 hours at 6.1 mmol/L point). Conclusion The tube addition of WZB117 is more suitable for minimizing glycolysis and has no effect on glucose levels even if specimens are left uncentrifuged for up to 3 hours.

Blood Glucose Determination without Deproteinization, with Use of o-Toluidine in Dilute Acetic Acid

1971 ◽  
Vol 17 (5) ◽  
pp. 440-441 ◽  
Author(s):  
Giovanni Ceriotti
Keyword(s):  
Acetic Acid ◽  
Blood Glucose ◽  
Acid Solution ◽  
Acetic Acid Solution ◽  
Glucose Determination ◽  
Blood Glucose Determination

Abstract A method is described for determining glucose in 25 µl of plasma. o-Toluidine is used in weak acetic acid solution. Addition of the emulsifier "Cremophor" obviates the need for deproteinization. The reaction is complete after 15 min at 100°C, and the color is stable for longer than 24 h. The blank is almost colorless. The reagent is stable, colorless, not viscous, and convenient to handle.

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