New ultraviolet (340 nm) method for assay of uric acid in serum or plasma.

1978 ◽  
Vol 24 (4) ◽  
pp. 562-566 ◽  
Author(s):  
R C Trivedi ◽  
L Rebar ◽  
K Desai ◽  
L J Stong
Keyword(s):  
Hydrogen Peroxide ◽  
Uric Acid ◽  
Alcohol Dehydrogenase ◽  
Linear Equation ◽  
New Method ◽  
Enzymatic Method ◽  
Sample Measurement ◽  
Enzymatic Methods

Abstract We propose a novel enzymatic method for assay of uric acid at 340 nm, which eliminates several disadvantages of both the colorimetric and enzymatic methods now in common use. Here, uric acid is catalytically oxidized to allantoin and hydrogen peroxide. The peroxide is reacted with ethanol in the presence of catalase to form acetaldehyde and water, and the acetaldehyde is reduced by NADH in the presence of alcohol dehydrogenase to ethanol. The decrease in absorbance at 340 nm caused by oxidation of NADH is directly proportional to the concentration of uric acid in the sample. Measurement of the change in absorbance between 20 and 200 s eliminates the need for a serum blank measurement. Absorbance and concentration are linearly related to 120 mg of uric acid per liter. The new method was compared with the uricase method in which decomposition of uric acid at 293 nm is directly measured. The results for the 47 patients' sera so examined can be expressed by the linear equation y340 = 1.0078x293 + 0.122 (r = 0.9984).

Use of a synthetic soluble bilirubin derivative to assess interference in creatinine measurements

1991 ◽  
Vol 37 (2) ◽  
pp. 236-238 ◽  
Author(s):  
Carlo Franzini ◽  
Anna M Morelli ◽  
Glanpaolo Cattozzo
Keyword(s):  
Hydrogen Peroxide ◽  
Serum Creatinine ◽  
Interference Effect ◽  
Nadh Oxidation ◽  
Enzymatic Method ◽  
Negative Interference ◽  
Significant Interference ◽  
Creatinine Measurement ◽  
Serum Creatinine Measurement ◽  
Enzymatic Methods

Abstract In assessing interference from bilirubin, the use of a synthetic soluble derivative (ditaurobilirubin, DTB) is recommended as a surrogate for the natural conjugates (Bc). We compared the interference effect of unconjugated bilirubin (Bu), Bc, and DTB, using six mechanized methods for serum creatinine measurement. No significant interference was noted in methods that include removal of proteins or in an enzymatic method involving NADH oxidation. Heavy (negative) interference was observed in an alkaline picrate method, and in direct enzymatic methods based on hydrogen peroxide measurement: interference was always more pronounced in the presence of the two soluble derivatives (Bc and DTB), whose interference was of the same magnitude. These results point out the utility of testing for bilirubin interference by using soluble derivatives, in addition to Bu, and suggest the feasibility of using DTB as a surrogate for Bc for this purpose.

New enzymatic method for serum uric acid at 500 nm.

1978 ◽  
Vol 24 (11) ◽  
pp. 1908-1911 ◽  
Author(s):  
R C Trivedi ◽  
L Rebar ◽  
E Berta ◽  
L Stong
Keyword(s):  
Hydrogen Peroxide ◽  
Uric Acid ◽  
Serum Uric Acid ◽  
Reference Method ◽  
Normal Serum ◽  
Enzymatic Method ◽  
Colored Complex ◽  
Separate Sample ◽  
Absorbance Measurement ◽  
Manual Method

Abstract We describe a manual method, well suited to mechanization, for quantitating serum uric acid at 500 nm. In the assay mixture (0.10 ml of sample and 3.00 ml of reagent) the hydrogen peroxide produced from uric acid by uricase is coupled with p-hydroxybenzoate and 4-aminoantipyrine in the presence of peroxidase to form a colored complex, which is measured. A separate sample blank is obviated by taking an initial absorbance measurement 20 s after the sample is added. The reaction is complete within 5 min; its sensitivity is 0.001 deltaA/mg per liter. Absorbances are linearly related to uric acid concentrations up to 120 mg/liter. Many substances that may be present in normal serum do not interfere, but bilirubin in moderately above-normal concentrations will interfere. The procedure can be modified to largely correct for this, when necessary. The proposed method (y) correlated well (r = 0.979) with the uric acid 293 nm reference method (x) and the relation is described by the equation y = 0.998x + 2.42.

Peroxidase-like reaction by a synergistic inorganic catalyst colloid: a new method for hydrogen peroxide detecting in air samples

2021 ◽  
Vol 299 (10) ◽  
pp. 1567-1575
Author(s):  
Zahra Moradpour ◽  
Mehrdad Helmi Kohnehshahri ◽  
Masoomeh Vahabi Shekarloo ◽  
Vahid Jalili ◽  
Rezvan Zendehdel
Keyword(s):  
Hydrogen Peroxide ◽  
New Method ◽  
Air Samples

scholarly journals Determination of uric acid in human serum by an enzymatic method using N-methyl-N-(4-aminophenyl)-3-methoxyaniline reagent

2003 ◽  
Vol 68 (8-9) ◽  
pp. 691-698 ◽  
Author(s):  
Milena Jelikic-Stankov ◽  
Predrag Djurdjevic ◽  
Dejan Stankov
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Limit Of Detection ◽  
Ascorbate Oxidase ◽  
Acid Oxidation ◽  
Uric Acid Concentration ◽  
Enzymatic Method ◽  
Limit Of Quantification ◽  
Relative Standard

In this work a new enzymatic method for the determination of uric acid in human serum has been developed. The method is based on the oxidative coupling reaction between the N-methyl-N-(4-aminophenyl)-3-methoxyaniline (NCP) reagent and the hydrogen ? donor reagent N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (TOOS), in the system involving three enzymes: uricase, peroxidase and ascorbate oxidase. Using this method uric acid could be determined in concentrations up to 1.428 mmol/L, with a relative standard deviation of up to 1.8 %. The effect of the medium pH and the NCP concentration on the linearity of the chromogen absorbance versus the uric acid concentration curve was investigated. The influence of the uricase activity on the maximum rate of uric acid oxidation was also examined. The use of the NCP reagent demonstrated a more precise and more sensitive determination of the uric acid compared to the determination with 4-aminoantipyrine (4-AA) as the coupling regent. The sensitivity of the method determined from the calibration curve was 0.71 absorbance units per mmol/L of uric acid; the limit of detection was LOD = 0.0035 mmol/L and the limit of quantification was LOQ = 0.015 mmol/L of uric acid.

scholarly journals Phenylboronic acid derivative-modified (6,5) single-wall carbon nanotube probes for detecting glucose and hydrogen peroxide

RSC Advances ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 2258-2267 ◽  
Author(s):  
Yunfan Qiao ◽  
Rushi Zhao ◽  
Min Zhang ◽  
Hongyang Zhang ◽  
Yuerong Wang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Carbon Nanotube ◽  
Acid Derivative ◽  
Near Infrared ◽  
Phenylboronic Acid ◽  
New Method ◽  
Fluorescence Probes ◽  
Single Wall Carbon Nanotube ◽  
Near Infrared Fluorescence ◽  
Single Wall Carbon

In this paper, we presented a new method for constructing near-infrared fluorescence probes and their applications in detecting glucose and hydrogen peroxide (H2O2).

A new method for the preparation of peroxymonophosphoric acid

2003 ◽  
Vol 81 (2) ◽  
pp. 156-160 ◽  
Author(s):  
Tian Zhu ◽  
Hou-min Chang ◽  
John F Kadla
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Carbon Tetrachloride ◽  
Glacial Acetic Acid ◽  
Acid Synthesis ◽  
Conversion Ratio ◽  
New Method ◽  
Phosphorous Pentoxide ◽  
Preparation Conditions ◽  
Peroxy Acids

A new method for the preparation of peroxymonophosphoric acid (H3PO5) has been developed. It utilizes a biphasic solution to moderate the vigorous reaction between phosphorous pentoxide (P2O5) and hydrogen peroxide (H2O2). P2O5 is suspended in carbon tetrachloride (CCl4), and concentrated H2O2 is slowly added while being vigorously stirred at low temperature. Careful control of the reaction temperature through the slow addition of H2O2 is critical. Using typical preparation conditions (P2O5:H2O2 = 0.5:1, H2O2 70 wt %, 2°C, 120–180 min), ~70% of the H2O2 is effectively converted to H3PO5. Increasing the concentration of H2O2, as well as the mole ratio of P2O5:H2O2, leads to an even higher % conversion of H2O2 to H3PO5. The addition of glacial acetic acid to the P2O5:H2O2 suspension at the end of the 120–180 min reaction (P2O5:H2O2:CH3COOH = 0.5:1:0.3) leads to the formation of peracetic acid in addition to H3PO5, and to an overall increase in the conversion ratio of total peroxy acids based on H2O2 (>95%).Key words: peroxymonophosphoric acid, synthesis, stability, conversion ratio.

Simultaneous determination of ascorbic acid, dopamine, uric acid and hydrogen peroxide based on co-immobilization of PEDOT and FAD using multi-walled carbon nanotubes

2014 ◽  
Vol 6 (20) ◽  
pp. 8321-8327 ◽  
Author(s):  
Kuo Chiang Lin ◽  
Jia Yan Huang ◽  
Shen Ming Chen
Keyword(s):  
Carbon Nanotubes ◽  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Uric Acid ◽  
Simultaneous Determination ◽  
High Conductivity ◽  
Hybrid Films ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Illustration of electro-codeposition of PEDOT and FAD hybrid films using high conductivity and steric MWCNTs as a template.

Gold nanocluster-based ratiometric fluorescent probes for hydrogen peroxide and enzymatic sensing of uric acid

2018 ◽  
Vol 185 (6) ◽  
Author(s):  
Dongqin Yang ◽  
Minchuan Luo ◽  
Junwei Di ◽  
Yifeng Tu ◽  
Jilin Yan
Keyword(s):  
Hydrogen Peroxide ◽  
Uric Acid ◽  
Fluorescent Probes ◽  
Gold Nanocluster
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