Effects of storage temperature and time before centrifugation on ionized calcium in blood collected in plain vacutainer tubes and silicone-separator (SST) tubes.

1984 ◽  
Vol 30 (4) ◽  
pp. 553-556 ◽  
Author(s):  
J Toffaletti ◽  
N Blosser ◽  
K Kirvan
Keyword(s):  
Room Temperature ◽  
Storage Temperature ◽  
Ionized Calcium ◽  
Blood Collection ◽  
Healthy Individuals ◽  
Blood Samples ◽  
The Mean ◽  
Whole Blood Samples ◽  
The Stability ◽  
Blood Collection Tubes

Abstract We studied the stability of ionized calcium and pH in samples stored at either room temperature or 4 degrees C, in centrifuged and uncentrifuged blood-collection tubes and in centrifuged tubes containing a silicone-separator gel (SST tubes). At room temperature, in uncentrifuged blood from healthy individuals, mean ionized calcium usually increased no more than 10 mumol/L per hour; at 4 degrees C it did not change detectably for 70 h. This stability was fortuitous, however: the concentrations of both hydrogen and lactate ions in these samples increased, apparently with offsetting effects on the concentration of ionized calcium. Blood stored for 70 h at 4 degrees C in centrifuged SST tubes, although showing a slightly greater change in ionized calcium, had less change of pH and no change in the ionized calcium corrected to pH 7.4. In 11 heparinized whole-blood samples from eight patients in intensive care, the mean change per hour in ionized calcium and pH after storage at room temperature was +10 mumol/L and -0.04 units, respectively.

scholarly journals Stability of Routine Hematology Sample Using The Medonic M-Series Analyzer

2020 ◽  
Vol 6 (2) ◽  
pp. 108
Author(s):  
Eva Ayu Maharani ◽  
Dewi Astuti
Keyword(s):  
Room Temperature ◽  
Storage Conditions ◽  
Healthy Individuals ◽  
Fresh Sample ◽  
Blood Samples ◽  
Significant Difference ◽  
Hematology Analyzer ◽  
Whole Blood Samples ◽  
The Stability ◽  
Wbc Count

Routine hematology tests (Hb, Hct, RBC, WBC, PLT) generally done by automation methods using a hematology analyzer. Ideally, the examinations should be done as soon as possible, although, in some circumstances, it can be delayed. Based on the literature, the sample for routine hematology testing should be processed within 4 – 8 hours of venesection. Some studies revealed that the sample could be stored for up to 48 hours, and it can be influenced by the technology applied to the hematology analyzer. Studies conducted to see the stability of the samples using a hematology analyzer with impedance technology. Tests performed on whole blood samples collected from 12 ostensibly healthy individuals, immediately after collection (fresh sample, <1hour) and 2h, 4h, 6h, 8h, 24h, 48h afterward. The samples stored at room temperature (20-240C) and 2-60C. There are no significant differences after 48 h under different storage conditions for Hb, Hct, RBC, PLT count, except for WBC count that has a significant difference at temperature 2-60C. CV for Hb, Hct, RBC, PLT, WBC count is less than 5% at room temperature. WBC and PLT count have a CV of more than 5% at 2-60 C. Sample for Hb, Hct, RBC was found to be stable up to 48 h at room temperature and 2-60C. PLT and WBC count were stable for 48 h if stored at room temperature.

scholarly journals Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
İbrahim Kaplan ◽  
Hatice Yüksel ◽  
Osman Evliyaoğlu ◽  
M. Kemal Basarali ◽  
Gülten Toprak ◽  
...  
Keyword(s):  
Cyclosporine A ◽  
Whole Blood ◽  
Room Temperature ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Blood Samples ◽  
Percent Change ◽  
Significant Difference ◽  
Whole Blood Samples ◽  
The Stability

Tacrolimus and cyclosporine A are immunosuppressant drugs with narrow therapeutic windows. The aim of this study was to investigate the stability of tacrolimus and cyclosporin A levels in whole blood samples under different storage conditions. Whole blood samples were obtained from 15 patients receiving tacrolimus and 15 patients receiving cyclosporine A. Samples were immediately analyzed and then stored at different conditions (room temperature (24°C−26°C) for 24 hours, +4°C for 24 and 48 hours, and −20°C for one month) and then analyzed again. For tacrolimus, there was a significant difference between samples analyzed immediately and those kept 24 hours at room temperature (P=0.005) (percent change 32.89%). However, there were no significant differences between the other groups. For cyclosporine A, there was a significant difference between samples analyzed immediately and those kept 24 hours (P=0.003) (percent change 19.47%) and 48 hours (P=0.002) (percent change 15.38%) at +4°C and those kept 24 hours at room temperature (P=0.011) (percent change 9.71%). Samples of tacrolimus should be analyzed immediately or stored at either +4°C or −20°C, while samples of cyclosporine A should be analyzed immediately or stored at −20°C.

scholarly journals Stability of Routine Hematology Sample Using The Medonic M-Series Analyzer

2020 ◽  
Vol 6 (2) ◽  
pp. 108
Author(s):  
Eva Ayu Maharani ◽  
Dewi Astuti
Keyword(s):  
Room Temperature ◽  
Storage Conditions ◽  
Healthy Individuals ◽  
Fresh Sample ◽  
Blood Samples ◽  
Significant Difference ◽  
Hematology Analyzer ◽  
Whole Blood Samples ◽  
The Stability ◽  
Wbc Count

Routine hematology tests (Hb, Hct, RBC, WBC, PLT) generally done by automation methods using a hematology analyzer. Ideally, the examinations should be done as soon as possible, although, in some circumstances, it can be delayed. Based on the literature, the sample for routine hematology testing should be processed within 4 – 8 hours of venesection. Some studies revealed that the sample could be stored for up to 48 hours, and it can be influenced by the technology applied to the hematology analyzer. Studies conducted to see the stability of the samples using a hematology analyzer with impedance technology. Tests performed on whole blood samples collected from 12 ostensibly healthy individuals, immediately after collection (fresh sample, <1hour) and 2h, 4h, 6h, 8h, 24h, 48h afterward. The samples stored at room temperature (20-240C) and 2-60C. There are no significant differences after 48 h under different storage conditions for Hb, Hct, RBC, PLT count, except for WBC count that has a significant difference at temperature 2-60C. CV for Hb, Hct, RBC, PLT, WBC count is less than 5% at room temperature. WBC and PLT count have a CV of more than 5% at 2-60 C. Sample for Hb, Hct, RBC was found to be stable up to 48 h at room temperature and 2-60C. PLT and WBC count were stable for 48 h if stored at room temperature.

Effect of transport conditions on the stability of biochemical markers in blood.

1989 ◽  
Vol 35 (12) ◽  
pp. 2313-2316 ◽  
Author(s):  
S E Hankinson ◽  
S J London ◽  
C G Chute ◽  
R L Barbieri ◽  
L Jones ◽  
...  
Keyword(s):  
Whole Blood ◽  
Apolipoprotein B ◽  
Biochemical Markers ◽  
Room Temperature ◽  
Density Lipoprotein ◽  
Alpha Tocopherol ◽  
Endogenous Hormones ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
The Stability

Abstract We examined the stability of lipids, carotenoids, alpha-tocopherol, and endogenous hormones in plasma prepared from whole blood that had been mailed to a central location for processing. Initially, to simulate transport conditions, whole-blood samples were stored in the laboratory, either at room temperature or cooled, for up to 72 h before processing. In the latter samples, lipid concentrations changed up to 1.4% per day, carotenoids up to -5.5%, and hormones up to 9.5%. In a second study, analyte concentrations in plasma from cooled whole blood mailed via overnight courier were compared with those from plasma that had been immediately separated, frozen, and mailed via overnight courier. Concentrations of cholesterol, high-density lipoprotein subfraction 3, apolipoprotein B, and retinol were stable. Overall, for each marker except estradiol, the between-person variation was at least twice the within-person variation. In a third study, at least 340 micrograms of DNA was recovered from 30 mL of cool-shipped whole blood. Our results indicate that shipping whole-blood samples by overnight courier is feasible for assay of several biochemical markers of interest in epidemiological research.

scholarly journals Effects of Storage and Type of Blood Collection Tubes on Hepatitis C Virus Level in Whole Blood Samples

2001 ◽  
Vol 39 (5) ◽  
pp. 1788-1790 ◽  
Author(s):  
H. H. Kessler ◽  
E. Stelzl ◽  
R. B. Raggam ◽  
J. Haas ◽  
F. Kirchmeir ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Whole Blood ◽  
Blood Collection ◽  
Blood Samples ◽  
Virus Level ◽  
Whole Blood Samples ◽  
Blood Collection Tubes

scholarly journals Formulation of Buprenorphine for Sublingual Use in Neonates

2011 ◽  
Vol 16 (4) ◽  
pp. 281-284 ◽  
Author(s):  
Ellena A. Anagnostis ◽  
Rania E. Sadaka ◽  
Linda A. Sailor ◽  
David E. Moody ◽  
Kevin C. Dysart ◽  
...  
Keyword(s):  
Room Temperature ◽  
Storage Time ◽  
Storage Temperature ◽  
Sublingual Administration ◽  
Tandem Mass ◽  
Abstinence Syndrome ◽  
Length Of Treatment ◽  
The Mean ◽  
The Stability ◽  
Length Of Hospitalization

OBJECTIVES The only medication used sublingually in the neonate is buprenorphine for the treatment of neonatal abstinence syndrome (NAS). Compared with morphine, buprenorphine reduces the length of treatment and length of hospitalization in neonates treated for NAS. The objective of this study was to characterize the stability of ethanolic buprenorphine for sublingual administration. METHODS Buprenorphine solution was prepared and stored in amber glass source bottles at either 68°F to 77°F (20°C-25°C) or 36°F to 46°F (2.2°C-7.8°C). Samples were collected from each of these batches on days 0, 3, 7, 14, and 30. Additional samples were withdrawn at baseline from each batch and placed in oral dispensing syringes for 3 and 7 days. Buprenorphine concentration was assessed by liquid chromatography–electrospray ionization–tandem mass spectrometry. RESULTS Neither storage temperature (p=0.65) nor storage time (p=0.24) significantly affected buprenorphine concentrations. All of the mean concentrations, regardless of storage temperature, were above 95% of the labeled concentration, and the potency was maintained for samples stored either in the original amber glass source bottles or in oral syringes. CONCLUSIONS An ethanolic buprenorphine solution is stable at room temperature for 30 days.

Evaluation of sample stability for cellular kinetics and pharmacodynamic flow cytometry methods

Bioanalysis ◽  
2019 ◽  
Vol 11 (20) ◽  
pp. 1881-1884 ◽  
Author(s):  
Vellalore N Kakkanaiah ◽  
Fan Pan ◽  
Patrick Bennett
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Room Temperature ◽  
Basophil Activation Test ◽  
Blood Collection ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Cellular Kinetics ◽  
Sample Stability ◽  
Blood Collection Tubes

We evaluated the sample stability for a cellular kinetics and a pharmacodynamic flow cytometry methods. First, the blood collection tubes were compared for the enumeration of chimeric antigen receptor-T cells in human whole blood. Blood samples with chimeric antigen receptor-T cells were stable up to 3 days at room temperature in both conventional EDTA and Cyto-Chex® blood collection tubes (Streck Laboratories, NE, USA), but with better consistency in Cyto-Chex-BCT than conventional EDTA tubes. Second, sample storage temperatures were compared for the basophil activation test in human whole blood samples. The samples were stable up to 3 days for basophil activation test when stored at refrigerator temperature, but not stable when stored at room temperature. It is crucial during the development of method to evaluate all the variables which might impact sample integrity.

Ascorbic Acid Improves the Stability of Buprenorphine in Frozen Whole Blood Samples

2019 ◽  
Vol 43 (6) ◽  
pp. 482-488 ◽  
Author(s):  
Lambert K Sørensen ◽  
Jørgen B Hasselstrøm
Keyword(s):  
Ascorbic Acid ◽  
Whole Blood ◽  
Storage Period ◽  
Blood Samples ◽  
The Mean ◽  
Whole Blood Samples ◽  
The Stability

Abstract Buprenorphine (BUP) was not long-term stable in whole blood samples preserved with fluoride citrate (FC) and fluoride oxalate (FX) mixtures when stored at −20°C. On average, only half of the initial concentrations of BUP was recovered after 12 weeks of storage at −20°C when interrupted by 3–4 thaw/freeze cycles. Norbuprenorphine (NBUP) was less unstable; approximately 90% was recovered after 12 weeks of storage at −20°C. The instability was less at 5°C, but the formation of BUP and NBUP from their glucuronides was observed at that temperature, especially in FC-preserved blood. The substances were stable for at least 5 months when stored uninterrupted at −80°C. The instability of BUP and NBUP in FC- and FX-preserved whole blood stored at −20°C was eliminated when the samples were modified with 30 mM ascorbic acid (ASC) prior to storage. The mean recoveries were greater than 95% after a 5-month interrupted storage period at −20°C when modified with ASC.

Facilitated determination of ionized calcium.

1985 ◽  
Vol 31 (2) ◽  
pp. 264-266 ◽  
Author(s):  
P Urban ◽  
B Buchmann ◽  
D Scheidegger
Keyword(s):  
Whole Blood ◽  
Ionized Calcium ◽  
Blood Samples ◽  
Intensive Care Patients ◽  
Therapeutic Measures ◽  
The Mean ◽  
Whole Blood Samples ◽  
Mean Concentration ◽  
Heparin Preparation

Abstract Using a calcium-containing heparin preparation for anticoagulation, we determined [Ca2+], the mean concentration of ionized calcium, in whole blood of 120 healthy blood-donors to be 1.23 (SD 0.04) mmol/L. Similarly, for 50 intensive-care patients selected without conscious bias, the correlation between [Ca2+] in serum (mean 1.15, SD 0.10 mmol/L) and in whole-blood samples anticoagulated with the same heparin preparation (mean 1.15, SD 0.09 mmol/L) was very good (r = 0.95). Storing samples anaerobically on ice for as long as 2 h did not alter whole-blood [Ca2+]. On the other hand, various concentrations of calcium-free heparin preparations all induced a significant decrease in measured [Ca2+]. By using whole-blood samples, rather than plasma or serum, for [Ca2+] determination with a calcium-selective electrode, repetitive measurements can be made with simple handling procedures, facilitating rapid implementation of appropriate therapeutic measures for critically ill patients.

scholarly journals Stability of parathyroid hormone ex vivo in haemodialysis patients

2003 ◽  
Vol 40 (2) ◽  
pp. 191-193 ◽  
Author(s):  
T. K. Teal ◽  
M. Reed ◽  
P. E. Stevens ◽  
E. J. Lamb
Keyword(s):  
Parathyroid Hormone ◽  
Room Temperature ◽  
Ex Vivo ◽  
Renal Dialysis ◽  
Blood Collection ◽  
Dialysis Patients ◽  
Edta Plasma ◽  
The Stability ◽  
End Stage ◽  
Blood Collection Tubes

Background: The stability of parathyroid hormone (PTH) in blood ex vivo is a significant practical problem for laboratories and clinicians. Several studies have suggested that PTH is more stable in blood collected into a potassium edetate (EDTA) preservative. Methods: To confirm that this was applicable to renal dialysis patients using our assay (Nichols chemiluminescence), we examined PTH stability in 13 patients with end-stage renal failure using three different blood collection tubes. Results: PTH remained stable in EDTA plasma for up to 48 h at room temperature. PTH was significantly reduced in serum collected into plain tubes after 2 h, and after 4 h in serum collected into serum separator tubes, at room temperature. Conclusion: In the assessment of renal osteodystrophy, the use of EDTA plasma can confer significant benefit, especially in busy laboratories where rapid frozen separation of blood may be hard to achieve.

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