Application of the MAST Immunodiagnostic System to the determination of allergen-specific IgE.

1984 ◽  
Vol 30 (9) ◽  
pp. 1467-1472 ◽  
Author(s):  
S P Miller ◽  
V A Marinkovich ◽  
D H Riege ◽  
W J Sell ◽  
D L Baker ◽  
...  
Keyword(s):  
High Speed ◽  
Clinical Laboratory ◽  
Solid Phase ◽  
Test Chamber ◽  
Specific Ige ◽  
Test System ◽  
Commercial Application ◽  
Test Results ◽  
Specificity And Sensitivity

Abstract The MAST Immunodiagnostic Test System was developed to provide a comprehensive, simple means for the in vitro measurement of multiple antigens or antibodies. The first commercial application of the MAST system incorporates several novel features for cost-effective diagnosis of IgE-mediated allergy in a clinical laboratory or a physician's office. The basis of the MAST system is a unique analytical test chamber, which contains cellulose thread as the solid-phase matrix and allows multiple test results from a single assay. This test chamber incorporates both positive and negative controls and requires no volume-dependent pipetting steps. Immunographic exposure onto high-speed Polaroid instant film allows for quantifying results with an automatic recording infrared-transmittance densitometer. Test results are easily interpreted by using a patient test record provided with the system. The MAST system greatly simplifies testing for allergen-specific IgE, while retaining specificity and sensitivity. Currently, with the MAST system one can simultaneously measure picomoles of allergen-specific IgE in up to 35 different allergen classes. In addition to allergy testing, the MAST technology is applicable to other immunodiagnostic profiles.

scholarly journals IN VITRO ALLERGY DIAGNOSIS: A COMPARATIVE ANALYSIS OF IgE SPECIFIC TO THE INHALANT ALLERGENS, OBTAINED BY IMMULITE AND PHADIA ASSAY SYSTEMS

2019 ◽  
Vol 21 (5) ◽  
pp. 997-1004
Author(s):  
E. V. Zueva ◽  
I. V. Khamitova ◽  
A. M. Melichkina ◽  
L. L. Lazarenko ◽  
L A. Bakanina ◽  
...  
Keyword(s):  
Skin Test ◽  
Method Comparison ◽  
Birch Pollen ◽  
Test System ◽  
Skin Tests ◽  
Laboratory Research ◽  
Test Results ◽  
Test Systems ◽  
Specificity And Sensitivity

Allergen-specific diagnostics is carried out on the basis of the data collection from the patient’s family and personal history, skin test results, provocative tests and laboratory research methods. Methods for determining specific IgE antibodies (sIgE) play a key role in confirming the diagnosis and identifying the pathogenetic mechanism of an immediate-type hypersensitivity to an allergen. The purpose of the study was to evaluate the results of determining sIgE for allergens of cat epithelium, house dust mite D. farinae and birch pollen in the blood serum of patients suffering from respiratory allergy, by comparing two methods of ImmunoCAP Phadia and 3gAllergy Immulite, as well as determining whether the results of these test systems are in concordance with the results of skin tests in the patients. The serum samples were obtained from patients of St. Petersburg adult outpatient clinics, who suffered from respiratory allergies (n = 50). The samples were analyzed in parallel in two laboratories, with each of the laboratories using single test systems. The retrospective skin test results were obtained from twenty six of the fifty selected patients. The inter-method comparison was conducted by determining the concordance of positive and negative results, correlation and regression analysis of sIgE results for each allergen and ROC-analysis to compare the diagnostic specificity and sensitivity of test systems in relation to the results of skin tests. This study showed that, in terms of agreement and contingency of the results, the Immulite test system had a close relationship with the Phadia test system. Both analysis of classes and quantitative sIgE analysis showed a good positive correlation from 0.79 to 0.99 (p < 0.0001) between the two test systems for all three allergens. High accuracy of coincidence in terms of sensitivity, area under the ROC curves (AUC) and cut-off threshold in both test systems was obtained for the D. farinae allergen. For allergens of cat epithelium and birch pollen, some differences between test systems were observed, i.e., sensitivity and AUC values were significantly higher in Immulite than in Phadia assay for both allergens.Thus, the inter-method comparison gave almost equivalent binary and quantitative results of the determination of sIgE antibodies to cat, tick and birch allergens. Comparison of in vitro test results, and their correlation with skin tests showed that the cat and birch in vitro antibody testing with Immulite assay was more closely connected with skin test results, than Phadia assay system.

A Comparison of Acti*Tip™ Allerg*E™ and Allerg*ENS™ System to Phadebas RAST® and Phadebas IgE PRIST® in Assaying Total IgE and Specific IgE in Human Serum

1988 ◽  
Vol 2 (3) ◽  
pp. 135-140 ◽  
Author(s):  
John T. Connell
Keyword(s):  
Solid Phase ◽  
Detection System ◽  
Specific Ige ◽  
In Vitro System ◽  
Ige Antibodies ◽  
Total Ige ◽  
In Vitro Systems ◽  
Specific Ige Antibodies ◽  
A New Technique

Two in vitro systems of measuring total IgE and specific IgE antibodies were compared using serum of patients suspected of having allergic rhinitis due to inhalation of grass pollen. One in vitro system was the Phadebas PRIST and RAST. The accuracy of these tests has been well defined and they were used as the standard. The Acti*Tip Allerg*E and Allerg*ENS system is a new technique utilizing a similar solid-phase immunologic methodology with an enzyme-based detection system. It has some advantages primarily related to the mechanics of performing the test and marked reduction in time for obtaining results. The correlation between the Phadebas and Acti*Tip systems was highly significant for measuring total IgE and specific IgE antibodies for grasses in serum.

Model Test Evaluation of High Speed Trimaran (HST) Sea Train Concept

2015 ◽  
Author(s):  
Igor Mizine ◽  
Gabor Karafiath
Keyword(s):  
High Speed ◽  
Primary Objective ◽  
Rolling Stock ◽  
Commercial Application ◽  
Test Results ◽  
Sea State ◽  
Individual Unit ◽  
Port Access ◽  
Transportation Applications ◽  
Hydrodynamic Factors

Sea Train is a longitudinal assembly of essentially similar size transportation units that increase the overall length resulting in a performance benefit to the system. Different types of mechanical connector hydrodynamic loads in a sea state have been the Achilles heel of platform assemblies operating on the sea surface. For example, Mobile Offshore Base project was discontinued in the early 1990s, partly because of the risk associated with extremely high connector loads. The paper describes the Sea Train concept that eliminates the need for a mechanical link type of connector between units, thus allowing for rapid automatic assembly/disassembly into Sea Train formation. All of the previously explored seatrain concepts resulted in a very long assembly of units, and the concepts with no yaw articulation between units were expected to have very large turning diameters. Therefore a bulbous above water cylindrical nose and socket were designed to allow yaw articulation. The proposed connectorless approach to Seatrain assembly with ball and open socket coupling will also allow for lateral articulation between units to enhance turning. Sea Train is a new concept for Military Sealift as well as for a broad spectrum of potential commercial marine transportation applications. The main advantages for Sealift are high speed and austere port access. A commercial application includes an America’s Marine Highway (AMH) Concept. A seatrain carrier for rolling stock envisioned along with the possibility of continuous coastal service that has units dropping off and other units joining up to deliver cargo. Recently, selected model tests and operational demonstration, were performed by NSWCCD at their indoor tow tank and their outdoor maneuvering facility at Tridelphia Reservoir. A combination of individual unit tow arrangements, control algorithms for propulsion and steering, and remote control operation were examined. In conjunction with the operational demonstration, towing tank tests were done to measure a number of hydrodynamic factors. The primary objective was to determine the magnitude of resistance. These test results have confirmed basic Sea Train expectations and are presented in the paper.

Friction and Wear Behaviors of CAPVD TiAlN Coating

2013 ◽  
Vol 645 ◽  
pp. 101-104
Author(s):  
Ai Hua Liu ◽  
Ze Shen Liu ◽  
Peng Zhang
Keyword(s):  
High Speed ◽  
Friction And Wear ◽  
Vapour Deposition ◽  
Test System ◽  
Test Results ◽  
Wear Properties ◽  
Tialn Coating ◽  
Cathodic Arc ◽  
Wear Tests ◽  
Ball On Disc

The paper reports friction and wear study of TiAlN coating at different speeds and loads. The coating was deposited on YG6 (WC+6 wt.% Co) cemented carbide by cathodic arc physical vapour deposition technique. Ball-on-disc wear tests were performed on the CETR UMT-2 test system against SiC balls. The coating character and test results were obtained through SEM, XRD and Wyko surface profilometer. The results are as follows: The value of friction of coefficients decreased and depth of wear tracks became smaller as speeds and loads increased. TiAlN coating showed excellent anti-wear properties at high speed and high load, and was more suitable for these similar working conditions.

Wear and Friction Properties of TiN,TiAlN and CrAlN PVD Coatings

2011 ◽  
Vol 697-698 ◽  
pp. 436-439
Author(s):  
Ai Hua Liu ◽  
Jian Xin Deng ◽  
Hui Zhang ◽  
Yun Song Lian ◽  
Jun Zhao
Keyword(s):  
High Speed ◽  
Test System ◽  
Hard Coatings ◽  
Wear Behaviour ◽  
Test Results ◽  
Pvd Coatings ◽  
Wear Rates ◽  
Wear And Friction ◽  
Wear Tests ◽  
Ball On Disc

The paper reports a comparative study of the tribological properties of the most employed hard coatings like TiN, TiAlN and CrAlN. Ball-on-disc wear tests were performed on the CETR UMT-2 test system against Si3N4balls with a relatively high speed of 100m/min. The coating character and test results were obtained through SEM, XRD and Wyko surface profilometer. The results are as follows: The friction coefficients of the three coatings are relatively high because of the superabundant wear debris during tests. The wear behaviour and wear tracks exist distinct differences for different coatings. As for wear rates, the sequence of three coatings is TiN>TiAlN>CrAlN, the combination property of CrAlN is the best.

scholarly journals Application of the Department of Health and Human Services proposed waived status requirements for in vitro diagnostic testing devices: case study

1997 ◽  
Vol 43 (9) ◽  
pp. 1610-1617 ◽  
Author(s):  
Sharon S Ehrmeyer ◽  
Ronald H Laessig
Keyword(s):  
Reference Range ◽  
Clinical Laboratory ◽  
Health Care Financing ◽  
Diagnostic Testing ◽  
Test System ◽  
Department Of Health ◽  
In Vitro Diagnostic ◽  
Clinical Laboratory Testing

Abstract The CLIA’88 classified all clinical laboratory testing as waived, moderate, or high complexity. The eight original waived tests were characterized as simple, accurate, error-free, risk-free, and suitable for home use by nonlaboratory professionals. The subjective nature of the classification process was challenged immediately. The Clinical Laboratory Improvement Advisory Committee asked the CDC and the Health Care Financing Administration to develop objective criteria that included assessment of performance by field-test and in-house data. We examined the efficacy of the CDC protocol with empirical data from the HemoCue B-Hemoglobin Test System® submission, to assess operator competency, intra-/interoperator and between-site imprecision, and accuracy. Non-laboratory-trained operators demonstrated 2–3% imprecision (40–200 g/L). Accuracy studies yielded a slope of 1.01, an intercept of 3.53 g/L, and r of 1.00 (52–230 g/L). Results met the protocol’s Tonks’ criterion for imprecision (less than one-fourth of the reference range).

scholarly journals Features of fungal sensitization in patients with asthma and cystic fibrosis: the results of a prospective study

2018 ◽  
Vol 15 (2) ◽  
pp. 11-16
Author(s):  
Y I Kozlova ◽  
E V Frolova ◽  
Y V Borzova ◽  
A V Sobolev ◽  
E V Burygina ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Specific Ige ◽  
Test System ◽  
Candida Spp ◽  
Ige Antibodies ◽  
Fungal Allergens ◽  
Patients At Risk ◽  
Fungal Sensitization

Background. To estimate the frequency of fungal sensitization and allergic bronchopulmonary aspergillosis in patients with asthma or cystic fibrosis. Methods. The study included 205 patients with asthma and 190 patients with cystic fibrosis. For detection of fungal sensitization specific IgE antibodies in the blood serum with «AllergoELISA-specific IgE» test system and biotinylated fungal allergens were determined. Mycological examination included microscopy and cultural study of respiratory biomaterials. According to the indications, a CAT scan of the chest was performed. Results. The incidence of fungal sensitization in patients with asthma was 35,6%, CI [29,1%; 42,6%], in patients with cystic fibrosis - 56,8% [49,5%; 64%]. In patients with severe asthma the main fungal allergens were Alternaria spp. (70%) and Aspergillus spp. (60%); in patients with cystic fibrosis: Candida spp. - 73%, Alternaria spp. - 34%, Aspergillus spp. - 27%. The incidence of allergic bronchopulmonary aspergillosis (ABPA) in patients with asthma was 5,3%, in patients with cystic fibrosis - 5,7%. Conclusion Early detection of ABPA in patients with asthma and cystic fibrosis is very important as it changes the therapeutic measures of patients’ management and prevents progression of bronchiectasis and respiratory failure. Determination of specific Aspergillus spp. IgE antibodies in vitro is a necessary stage of diagnosis of ABPA in patients at risk.

scholarly journals Influence of polyelectrolytes on increasing sensitivity of an immunofluorescent analysis based on plasmon silver nanoparticles

2021 ◽  
pp. 72-80
Author(s):  
Irina V. Koktysh ◽  
Yanina I. Mel’nikova ◽  
Olga S. Kulakovich ◽  
Andrei A. Ramanenka ◽  
Sergey A. Maskevich
Keyword(s):  
Silver Nanoparticles ◽  
Solid Phase ◽  
Specific Antigen ◽  
Test System ◽  
Prostatic Specific Antigen ◽  
Immunofluorescence Test ◽  
Specificity And Sensitivity ◽  
Detectable Concentration ◽  
Immunochemical Test

It was studied the dependence of the interaction of the components of the immunofluorescence test system for the quantitative determination of prostate specific antigen based on plasmon silver nanoparticles on the physicochemical nature of various polyelectrolytes used to coat films of silver nanoparticles. It has been shown that the use of a weakly charged polycationic polyelectrolyte poly-L-lysine can increase the antigenic binding of the test system by 2.34 times, and the use of a highly charged polycationic polyelectrolyte polydiallyldimethylammonium chloride increases the binding affinity of prostatic specific antigen by 5 times. When developing various immunochemical test systems using films of silver nanoparticles, an important parameter is the choice of a polyelectrolyte for coating a silver nanolayer, since the physicochemical and electrostatic properties of the polyelectrolyte can significantly affect both the sorption capacity of the solid phase and the conformational state functional activity of immobilized protein molecules. Both specificity and sensitivity of the immunochemical test system, as well as the minimum possible detectable concentration of bioanalyte, largely depend on these parameters.

Simultaneous determination of total IgE and allergen-specific IgE in serum by the MAST chemiluminescent assay system.

1985 ◽  
Vol 31 (9) ◽  
pp. 1500-1505 ◽  
Author(s):  
C R Brown ◽  
K W Higgins ◽  
K Frazer ◽  
L K Schoelz ◽  
J W Dyminski ◽  
...  
Keyword(s):  
High Speed ◽  
Light Output ◽  
Specific Ige ◽  
Commercial Application ◽  
Serum Samples ◽  
Visible Image ◽  
Total Ige ◽  
Standard Curve ◽  
Sensitivity Specificity ◽  
Negative Controls

Abstract We have developed a chemiluminescent immunoenzymometric system. The first commercial application of this chemiluminescent assay (CLA) is the measurement of total IgE and allergen-specific IgE in human serum. The CLA system is a second-generation adaptation of the MAST RIA allergy profiling system. The MAST CLA system assay protocol consists of three steps: overnight incubation of serum, a 4-h incubation with enzyme-labeled antibody, and a 30-min chemiluminescent reaction, which produces a visible image (immunograph) on high-speed Polaroid instant film. The densities of the bands produced on the film are quantified with an inexpensive microprocessor-controlled infrared transmittance densitometer. The novel luminogenic substrates used yield a constant light output for over 2 h with an intensity at least 10-fold greater than that of commercial chemiluminescent reagents. The MAST CLA system exhibits sensitivity, specificity, and precision equal to that of the MAST RIA system (r = 0.96 for 40 serum samples analyzed with 25 allergens). As many as 35 different allergens per sample can be quantified in a single assay. The MAST CLA system requires no standard curve or volume-dependent pipetting steps, incorporates both positive and negative controls for each sample, and quantifies allergen-specific IgE at picomolar concentrations.

Possibilities of using new test system for screening antibodies to hepatitis C produced by Alkor Bio company by ELISA during comparative laboratory tests

2020 ◽  
pp. 14-18
Author(s):  
T. D. Grigorieva ◽  
M. Yu. Falileeva ◽  
E. P. Shargorodskaya
Keyword(s):  
Continuous Improvement ◽  
Laboratory Tests ◽  
Clinical Laboratory ◽  
Test System ◽  
Laboratory Diagnostics ◽  
Analytical Sensitivity ◽  
Test Systems ◽  
Specificity And Sensitivity ◽  
Infectious Disease Specialists ◽  
Real Patients

Currently, despite the wide variety of methods and tests for screening blood serum for antibodies to HCV, there are no reliable criteria that guarantee a reliable result. To date, the interpretation of the results of anti-HCV detection in samples with low CP values (low optical density in ELISA) remains one of the major tasks of laboratory diagnostics. Continuous improvement of analytical sensitivity and specificity of laboratory tests remains one of the priority tasks for manufacturers of modern test systems. The purpose of this article was to assess the specificity and sensitivity of the new Hepatitis ELISA-anti-HCV test system produced by Alkor Bio when compared with test systems from other manufacturers in settings with sera from real patients and in a series of control seroconversion panels. The article is intended for doctors of clinical laboratory diagnostics, epidemiologists, virologists, infectious disease specialists, students of medical and biological universities.

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