Immunoenzymometric determination of trypsin/alpha 1-protease inhibitor complex in plasma of rats with experimental pancreatitis.

1986 ◽  
Vol 32 (9) ◽  
pp. 1738-1741 ◽  
Author(s):  
T S Fletcher ◽  
H Tsukamoto ◽  
C Largman
Keyword(s):  
Protease Inhibitor ◽  
Solid Phase ◽  
Gel Filtration ◽  
Experimental Pancreatitis ◽  
Size Fractionation ◽  
Single Tube ◽  
Inhibitor Complex ◽  
Cationic Trypsin ◽  
High Concentrations

Abstract The currently available radioimmunoassays of trypsin measure total immunoreactive trypsin (EC 3.1.1.7), which includes both trypsinogen and alpha 1-protease inhibitor-bound trypsin. Hitherto, the only way to differentiate these two forms of trypsin has been to fractionate them on a gel-filtration column. We describe here a solid-phase immunoenzymometric assay that rapidly measures the amount of cationic trypsin bound to alpha 1-protease inhibitor in the plasma of rats with experimental pancreatitis. The assay specifically measures this complex within the range from 0.2 to 5.0 ng without interference by high concentrations of free alpha 1-protease inhibitor. The high correlation (r = 0.985) of the values obtained by size fractionation and by this assay demonstrates the accuracy of the assay, which is the first single-tube method for determining this form of activated cationic trypsin in plasma.

A new reagent strip (Visidex) for determination of glucose in whole blood.

1983 ◽  
Vol 29 (3) ◽  
pp. 438-446 ◽  
Author(s):  
M J Sherwood ◽  
M E Warchal ◽  
S T Chen
Keyword(s):  
Whole Blood ◽  
Solid Phase ◽  
Sample Volume ◽  
Operator Technique ◽  
Good Precision ◽  
Visual Resolution ◽  
Color Development ◽  
Color Scale ◽  
High Concentrations

Abstract A new solid-phase reagent strip for determination of glucose in whole blood, Visidex, has been developed specifically for visual use in conjunction with a calibrated color scale. Two reagent pads are used, each formulated for a different portion of the range of clinical values, to maximize the visual resolution available to the user. Colorimetric examination of reacted reagent pads indicated that the label color blocks match closely the appearance of the reagent pads; that the reagent pads exhibit good precision; and that the colors of the reagent pads are independent of operator technique, sample volume (20-50 microL), and effects of potential interferents studied (although high concentrations of fluoride slightly inhibited color development). Glucose measurements obtained visually with the Visidex system correlated well with values obtained with a YSI Glucose Analyzer (for two separate studies, slope = 0.96 and 1.04, r = 0.99 and 0.96, and n = 172 and 543, respectively).

scholarly journals Occurrence and risk assessment of parabens and triclosan in surface waters of southern Brazil: a problem of emerging compounds in an emerging country

RBRH ◽  
2016 ◽  
Vol 21 (3) ◽  
pp. 603-617 ◽  
Author(s):  
Mauricius Marques dos Santos ◽  
Franciane de Almeida Brehm ◽  
Tais Cristina Filippe ◽  
Heloise Garcia Knapik ◽  
Júlio César Rodrigues de Azevedo
Keyword(s):  
Risk Assessment ◽  
Solid Phase ◽  
Aquatic Organisms ◽  
Human Action ◽  
Pharmaceutical Products ◽  
Metropolitan Region ◽  
Southern Brazil ◽  
Emerging Compounds ◽  
High Concentrations

ABSTRACT Parabens and triclosan compounds are widely used in pharmaceutical products, personal care, food and various products of daily use. After use, the final destinations of these contaminants are domestic effluents and the environment if not collected or treated. The objective of this study was to evaluate the presence of parabens and triclosan in environments in a subtropical region, more specifically in the metropolitan region of Curitiba (southern Brazil) and possible effects of these compounds in aquatic environments through risk assessment through the use of risk quotient (RQ). Samples were collected in 20 points for 4 campaigns. For the extraction of the compounds we used the extraction technique of solid phase extraction (SPE). The determination of the compounds was using gas chromatography coupled to mass spectrometry (GC-MS). It was observed high concentrations of parabens, especially MeP (up to 2875 ng L–1). Despite high concentrations of parabens, their presence has a low risk to aquatic organisms. Triclosan has been observed at higher concentrations (415 ng L–1), suggesting once again that these environments are impacted by human action. The RQ assessed that triclosan has a high risk for certain groups of organisms.

scholarly journals Determination of Homocysteine in Urine and Saliva by Microchip Electrophoresis

2018 ◽  
Vol 46 (1) ◽  
pp. 43-46
Author(s):  
Peter Troška ◽  
Anna Mandžáková ◽  
Jasna Hradski ◽  
Juraj Ševčík ◽  
Marián Masár
Keyword(s):  
Solid Phase ◽  
Sample Pretreatment ◽  
Injection Technique ◽  
High Concentration ◽  
Complex Samples ◽  
Capillary Zone ◽  
Sensitive Determination ◽  
High Concentrations ◽  
Trace Concentrations

Abstract An online combination of isotachophoresis (ITP) and capillary zone electrophoresis (CZE) was employed on a microchip with a column-coupling technology for the determination of homocysteine in various body fluids. ITP with its high concentration ability was used as a sample pretreatment and injection technique for CZE, which facilitated the rapid and sensitive determination of homocysteine. The resolution of the analyte from other constituents present in real complex samples was enhanced by discrete spacers, which were added to the injected sample. A solid-phase microextraction (SPME) pretreatment technique based on silver- and barium-form resins was used prior to the ITP-CZE analysis to remove high concentrations of chloride and sulfate naturally present in the analyzed samples. The combination of the micropretreatment and microelectrophoresis techniques facilitated the determination of trace concentrations of homocysteine in samples of urine and saliva

Determination of the novel non-peptidic HIV-protease inhibitor tipranavir by HPLC–UV after solid-phase extraction

2006 ◽  
Vol 832 (1) ◽  
pp. 138-143 ◽  
Author(s):  
S. Colombo ◽  
A. Béguin ◽  
C. Marzolini ◽  
A. Telenti ◽  
J. Biollaz ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Protease Inhibitor ◽  
Solid Phase ◽  
Hiv Protease ◽  
Phase Extraction ◽  
The Novel ◽  
Hiv Protease Inhibitor

Trypsin activation of human and cat prorenin: a comparative study

1991 ◽  
Vol 69 (9) ◽  
pp. 1385-1389 ◽  
Author(s):  
Speranza Rubattu ◽  
Fuad Gahnem ◽  
Jean E. Sealey
Keyword(s):  
Liquid Phase ◽  
Comparative Study ◽  
Protease Inhibitor ◽  
Serine Protease ◽  
Species Differences ◽  
Solid Phase ◽  
Limited Proteolysis ◽  
Serine Protease Inhibitor ◽  
Inactive Renin ◽  
High Concentrations

Prorenin can be converted to renin by limited proteolysis with trypsin. In the current study we compared conditions for activation of human renal and ovarian prorenin and cat renal prorenin with either liquid-phase trypsin or trypsin bound to sepharose (solid phase). Higher concentrations of trypsin were required to activate cat prorenin than human prorenin. Human prorenin was destroyed by high concentrations of trypsin, while cat prorenin was not destroyed by up to 2 mg/mL solid-phase trypsin. For both human and cat prorenin, addition of the competitive serine protease inhibitor benzamidine–HCl increased the concentration of trypsin needed to activate prorenin, resulting in slightly higher levels of human prorenin but lower levels of cat prorenin. For human samples, activation with solid-phase trypsin resulted in slightly higher estimates of prorenin than liquid-phase trypsin. These results demonstrate species differences in the susceptibility of prorenin to trypsin cleavage. Cat prorenin requires more trypsin to be activated and is less susceptible to destruction than human prorenin.Key words: prorenin, inactive renin, cat human, trypsin.

Modern determinants of endometrium hyperplastic processes pathogenesis

2016 ◽  
pp. 137-142
Author(s):  
V.O. Benyuk ◽  
◽  
V.M. Goncharenko ◽  
T.R. Nykoniuk ◽  
◽  
...  
Keyword(s):  
Conservative Therapy ◽  
Solid Phase ◽  
Research Direction ◽  
Immunohistochemical Method ◽  
Results Of Treatment ◽  
Treatment And Prevention ◽  
Receptor Phenotype ◽  
The Relationship ◽  
Endometrial Proliferation

The objective: to еxplore the relationship between the activity of endometrial proliferation and the state of the local immune response in the uterus in the conditions berprestasi process. Patients and methods. Examined 228 women of reproductive and perimenopausal age with endometrial pathology using ultrasound and then performing hysteroresectoscopy. Determination of the concentrations of the cytokines IL-1, IL-2, IL-6 and TNF was performed by solid phase ELISA. Results. Found a trend that confirms the loss of sensitivity to hormones at the stage of malignancy of the endometrium and can be used as diagnostic determinants in determining the nature of intrauterine pathology and criterion of the effectiveness of conservative therapy. Conclusion. Improving etiopatogenetice approach to the therapy of hyperplastic proce.sses of endometrium with determination of receptor phenotype of the endometrium is a research direction in modern gynecology, which will help to improve the results of treatment and prevention of intrauterine pathology. Key words: endometrial hyperplasia,the receptors for progesterone and estrogen, immunohistochemical method.

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