Enzymatic method for selective determination of 4-aminobenzoic acid in urine

1990 ◽  
Vol 36 (11) ◽  
pp. 1937-1940 ◽  
Author(s):  
N Bando ◽  
T Ogawa ◽  
H Tsuji ◽  
K Sasaoka
Keyword(s):  
Pancreatic Function ◽  
Urine Samples ◽  
Enzymatic Method ◽  
Aminobenzoic Acid ◽  
Selective Determination ◽  
Subsequent Conversion ◽  
Colorimetric Assays ◽  
Colorimetric Methods

Abstract We developed a method for the selective determination of 4-aminobenzoic acid by use of 4-aminobenzoate hydroxylase (EC 1.14.13.27) to convert 4-aminobenzoic acid to 4-hydroxyaniline. The subsequent conversion of 4-hydroxyaniline to indophenol dye was quantified colorimetrically. The method is reproducible, and the assay response is linear up to 40 mumol/L. Selectivity exceeding that of the current colorimetric assays was demonstrated for these enzymatic determinations of 4-aminobenzoic acid concentrations in urine samples from patients undergoing tests of pancreatic function with bentiromide. This method effectively minimizes interferences from drugs and diet, a problem in current colorimetric methods.

Ultra-sensitive and selective determination of a phenolic food additive using protein capped gold nanoclusters: a dual in-line fluorometric and colorimetric sensing probe

2021 ◽  
Author(s):  
Sekar Shankar ◽  
N. S. K. Gowthaman ◽  
P. Arul ◽  
Feng Chen ◽  
H. N. Lim ◽  
...  
Keyword(s):  
Gold Nanoclusters ◽  
Food Additive ◽  
Colorimetric Sensing ◽  
Selective Determination ◽  
Red Luminescence ◽  
Colorimetric Methods

The application of red luminescence BSA-AuNCs towards the selective determination of food additive tert-butylhydroquinone (TBHQ) was demonstrated by both fluorometric and colorimetric methods.

Group-selective determination of total N-nitroso compounds in nitrate-containing human urine samples

The Analyst ◽  
1989 ◽  
Vol 114 (9) ◽  
pp. 1103 ◽  
Author(s):  
Brigitte Pignatelli ◽  
Chong-Sheng Chen ◽  
Pascal Thuillier ◽  
Helmut Bartsch
Keyword(s):  
Human Urine ◽  
Urine Samples ◽  
Nitroso Compounds ◽  
Total N ◽  
Selective Determination ◽  
Human Urine Samples

Application of modifier-free gold nanoparticle colorimetric sensing for rapid screening and detection of vitamin B1

2020 ◽  
Vol 44 (22) ◽  
pp. 9223-9229 ◽  
Author(s):  
Paweenar Duenchay ◽  
Kantima Kaewjua ◽  
Orawon Chailapakul ◽  
Weena Siangproh
Keyword(s):  
Gold Nanoparticle ◽  
Vitamin B1 ◽  
Urine Samples ◽  
Rapid Screening ◽  
Colorimetric Sensing ◽  
Selective Determination ◽  
Sensing Platform ◽  
Free Gold

A simple and fast alternative colorimetric sensing platform for selective determination of vitamin B1 in urine samples was proposed.

New voltammetric analysis of olanzapine in tablets and human urine samples using a modified carbon paste sensor electrode incorporating gold nanoparticles and glutamine in a micellar medium

2015 ◽  
Vol 7 (2) ◽  
pp. 581-589 ◽  
Author(s):  
Hytham M. Ahmed ◽  
Mona A. Mohamed ◽  
Waheed M. Salem
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Sensor ◽  
Human Urine ◽  
Urine Samples ◽  
Micellar Medium ◽  
Carbon Paste ◽  
Voltammetric Analysis ◽  
Selective Determination ◽  
Sensor Electrode

An effective novel electrochemical sensor for the selective determination of olanzapine (OLA) was introduced.

Multiwall carbon nanotube ensembled biopolymer electrode for selective determination of isoniazid in vitro

2014 ◽  
Vol 6 (11) ◽  
pp. 3772-3778 ◽  
Author(s):  
M. Satyanarayana ◽  
K. Koteshwara Reddy ◽  
K. Vengatajalabathy Gobi
Keyword(s):  
Carbon Nanotube ◽  
Electrochemical Biosensor ◽  
Urine Samples ◽  
Multiwall Carbon Nanotube ◽  
Selective Determination ◽  
Artificial Urine ◽  
Sensitive Determination ◽  
Multiwall Carbon

A reagent-free electrochemical biosensor is fabricated for the sensitive determination of the important anti-tubercular drug isoniazid directly from spiked drug formulations and undiluted artificial urine samples.

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