scholarly journals Detection of α2u-globulin in rat pup preputial gland by MALDI-TOF mass spectrometry

2009 ◽  
Vol 55 (4) ◽  
pp. 296-300 ◽  
Author(s):  
Ponnirul Ponmanickam ◽  
Gnanasekaran Jebamercy ◽  
Govindaraju Archunan ◽  
Soundrapandian Kannan
Keyword(s):  
Mass Spectrometry ◽  
Male Rat ◽  
Preputial Gland ◽  
Adult Rats ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Pheromonal Communication ◽  
Adult Male Rat ◽  
Prepubertal Rats ◽  
Preputial Glands

Abstract The α2u-globulin, a soluble protein identified in the urine and preputial gland of adult male rat is reported to be pheromone carrier. The pup preputial gland plays a significant role in chemical communication for mother-young interaction; however, the presence of a pheromone-carrying protein in the pup preputial gland has not been confirmed. Therefore, the present study was carried out to identify the α2u-globulin in the pup preputial gland by Matrix Assisted Laser Desorption Ionization Time of Flight mass spectrometry (MALDI-TOF). The preputial glands of prepubertal rats were subjected to one-dimensional SDS-PAGE. In-gel trypsin digestion of a 18 kDa band was carried out and analyzed by MALDI-TOF. The results of a MASCOT search showed the presence of α2u-globulin in the 18 kDa band. In contrast to the report of the synthesis of this protein only in adult rats, the identification of this protein in pup preputial gland is significant. The results suggest that synthesis of α2u-globulin in the rat preputial gland starts in the prepubertal stage itself. In prepubertal rats, the preputial gland is a source of pheromone for performing anogenital licking behaviour by the mother rat. Since α2u-globulin belongs to the lipocalin (ligand carrier) family, it might carry the volatile for processing pheromonal communication in mother-pup bonding in rat.

scholarly journals Construction and Application of MALDI-TOF Mass Spectrometry for the Detection of Haemophilus parasuis

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Xiaoxu Wang ◽  
Feng Xu ◽  
Kun Ning ◽  
Liping Shen ◽  
Xinyong Qi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Clinical Diagnosis ◽  
Culture Media ◽  
The Self ◽  
Haemophilus Parasuis ◽  
Ionization Time ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Fingerprint Database ◽  
Protein Fingerprint

To construct a protein fingerprint database of Haemophilus parasuis (H. parasuis), thus improving its clinical diagnosis efficiency. A total of 15 H. parasuis standard strains were collected to establish a protein fingerprint database of H. parasuis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the effects of different culture media and culture time on the quality and identification results of the protein fingerprint were investigated. The results showed that tryptone soy agar (TSA) and tryptone soy broth (TSB) media and different incubation times had no significant effect on the characteristic peaks of the protein profiles. In addition, 18 clinical isolates were used to compare the identification results of the self-built protein fingerprint database, PCR detection, and basic database. Only one strain was identified in the original VITEK-MS system database, while the self-made protein fingerprint database of H. parasuis was 100% accurate for the detection of 18 clinical isolate strains. The protein fingerprint database of H. parasuis built by our laboratory is suitable for rapid clinical diagnosis of H. parasuis, due to its high accuracy, efficiency, and strong specificity.

scholarly journals Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology

2014 ◽  
Vol 8 (09) ◽  
pp. 1081-1088 ◽  
Author(s):  
Elena De Carolis ◽  
Antonietta Vella ◽  
Luisa Vaccaro ◽  
Riccardo Torelli ◽  
Teresa Spanu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Susceptibility ◽  
Biochemical Tests ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Bacteria And Fungi ◽  
Diagnostic Microbiology ◽  
Tof Ms

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled. In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures. Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection.

scholarly journals Identification and dereplication of endophytic Colletotrichum strains by MALDI TOF mass spectrometry and molecular networking

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Morgane Barthélemy ◽  
Vincent Guérineau ◽  
Grégory Genta-Jouve ◽  
Mélanie Roy ◽  
Jérôme Chave ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Study ◽  
Chemical Diversity ◽  
Biologically Active ◽  
Ionization Time ◽  
Ion Clusters ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Palm Species ◽  
Ethyl Acetate Extracts

AbstractThe chemical diversity of biologically active fungal strains from 42 Colletotrichum, isolated from leaves of the tropical palm species Astrocaryum sciophilum collected in pristine forests of French Guiana, was investigated. The collection was first classified based on protein fingerprints acquired by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) correlated with cytotoxicity. Liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-HRMS/MS) data from ethyl acetate extracts were acquired and processed to generate a massive molecular network (MN) using the MetGem software. From five Colletotrichum strains producing cytotoxic specialized metabolites, we predicted the occurrence of peptide and cytochalasin analogues in four of them by MN, including a similar ion clusters in the MN algorithm provided by MetGem software. Chemoinformatics predictions were fully confirmed after isolation of three pentacyclopeptides (cyclo(Phe-Leu-Leu-Leu-Val), cyclo(Phe-Leu-Leu-Leu-Leu) and cyclo(Phe-Leu-Leu-Leu-Ile)) and two cytochalasins (cytochalasin C and cytochalasin D) exhibiting cytotoxicity at the micromolar concentration. Finally, the chemical study of the last active cytotoxic strain BSNB-0583 led to the isolation of four colletamides bearing an identical decadienamide chain.

scholarly journals MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens

2019 ◽  
Vol 7 (12) ◽  
pp. 593 ◽  
Author(s):  
Maureen Feucherolles ◽  
Henry-Michel Cauchie ◽  
Christian Penny
Keyword(s):  
Mass Spectrometry ◽  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Reference Method ◽  
Multi Drug Resistance ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
The Future ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is today the reference method for direct identification of microorganisms in diagnostic laboratories, as it is notably time- and cost-efficient. In the context of increasing cases of enteric diseases with emerging multi-drug resistance patterns, there is an urgent need to adopt an efficient workflow to characterize antimicrobial resistance (AMR). Current approaches, such as antibiograms, are time-consuming and directly impact the “patient-physician” workflow. Through this mini-review, we summarize how the detection of specific patterns by MALDI-TOF MS, as well as bioinformatics, become more and more essential in research, and how these approaches will help diagnostics in the future. Along the same lines, the idea to export more precise biomarker identification steps by MALDI-TOF(/TOF) MS data towards AMR identification pipelines is discussed. The study also critically points out that there is currently still a lack of research data and knowledge on different foodborne pathogens as well as several antibiotics families such as macrolides and quinolones, and many questions are still remaining. Finally, the innovative combination of whole-genome sequencing and MALDI-TOF MS could be soon the future for diagnosis of antimicrobial resistance in foodborne pathogens.

scholarly journals First Isolation of Exiguobacterium aurantiacum in Serbia

2021 ◽  
Vol 70 (3) ◽  
pp. 405-407
Author(s):  
VERA P. GUSMAN ◽  
DEANA D. MEDIĆ ◽  
ANIKA DJ TRUDIĆ ◽  
PAVLE Z. BANOVIĆ ◽  
NATAŠA M. NIKOLIĆ
Keyword(s):  
Mass Spectrometry ◽  
Environmental Samples ◽  
Automated System ◽  
Distilled Water ◽  
Immunocompromised Patients ◽  
Bacterial Strains ◽  
Ionization Time ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Rare Bacteria

Exiguobacterium aurantiacum is isolated from a variety of environmental samples but rarely from patients. The aim of the study was to represent isolation of unusual bacterial strains that could cause infection in patients. Final identification was performed using matrix-assisted description/ionization time-of-flight mass spectrometry (MALDI-TOF). Two isolates strains of E. aurantiacum were isolated, one isolate from distilled water used during surgical treatment and the second one from a patient with bacteremia after radical prostatectomy, both sensitive to all tested antimicrobials. Environmental strains could cause infection, especially in immunocompromised patients; therefore, rare bacteria testing is required, in which identification special assistance is provided by an automated system MALDI-TOF.

scholarly journals Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) in clinical mircobiological routine diagnostics1)

2011 ◽  
Vol 35 (4) ◽  
pp. ---
Author(s):  
Sören Schubert ◽  
Andreas Wieser
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tract Infections ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Abstract Very recently a novel method for differentiation of bacteria and fungi was developed, that is, identification by means of matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS). This differentiation relies on the exact measurement of species-specific protein spectra of ribosomal proteins. It is at least as accurate as conventional biochemical differentiation methods, but provides results within minutes. In addition to differentiation of bacteria and yeasts grown on agar plates, direct identification is feasible from positive blood cultures as well as from urine samples of patients suffering from urinary tract infections. Future developments of MALDI-TOF MS for clinical microbiological purposes include the detection of β-lactamase and carbapenemase activity as well as genotyping of bacteria below the species level.

scholarly journals High mass resolution MALDI-TOF MS for profiling biomolecules in mixtures

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
High Sensitivity ◽  
Mass Resolution ◽  
High Mass ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
High Mass Resolution ◽  
Tof Ms

Intact biomolecules carry its identity through its atomic constituents and mass, while fragmented biomolecules require reconstruction for their identity to be retrieved. Hence, for profiling biomolecules in mixtures, the goal would be the gentle ionization of biomolecules by mass spectrometry without inducing fragmentation. Doing so generates an ensemble of ionized intact biomolecules able to be profiled by high sensitivity time-of-flight detector for accurate determination of each biomolecule mass, and thus, identity. Specifically, in time-of-flight detection, high mass resolution determination would require high sensitivity in detecting small differences in time of arrival of biomolecule ions to the detector. While current time-of-flight mass spectrometry provides high mass resolution, greater mass resolution is needed for discriminating different biomolecules in a mixture, where mass differences between biomolecules could be at the sub-Dalton level. With the ability to reliably detect biomolecules with sub-Dalton mass resolution, mass spectrometry with time-of-flight detector such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) could find use in identifying the compendium of biomolecules present in a mixture without tedious and time-consuming separation. The larger question would subsequently be coupling sample preparation needs with the conditions conducive for MALDI-TOF MS analysis. Overall, high mass resolution mass spectrometry techniques for profiling biomolecules would find use as an enabling tool in many areas of analytical science and biological sciences such as proteomics and metabolomics.

Sign in / Sign up

Export Citation Format

Share Document