scholarly journals ForageGrassBase: molecular resource for the forage grass meadow fescue (Festuca pratensis Huds.)

Database ◽  
2020 ◽  
Vol 2020 ◽  
Author(s):  
Jeevan Karloss Antony Samy ◽  
Odd Arne Rognli ◽  
Mallikarjuna Rao Kovi
Keyword(s):  
Draft Genome ◽  
Grass Species ◽  
Comparative Genomic ◽  
Festuca Pratensis ◽  
Forage Grass ◽  
Forage Grasses ◽  
Meadow Fescue ◽  
Crop Species ◽  
Genome Database ◽  
Genomic Resources

Abstract Meadow fescue (Festuca pratensis Huds.) is one of the most important forage grasses in temperate regions. It is a diploid (2n = 14) outbreeding species that belongs to the genus Festuca. Together with Lolium perenne, they are the most important genera of forage grasses. Meadow fescue has very high quality of yield with good winter survival and persistency. However, extensive genomic resources for meadow fescue have not become available so far. To address this lack of comprehensive publicly available datasets, we have developed functionally annotated draft genome sequences of two meadow fescue genotypes, ‘HF7/2’ and ‘B14/16’, and constructed the platform ForageGrassBase, available at http://foragegrass.org/, for data visualization, download and querying. This is the first open-access platform that provides extensive genomic resources related to this forage grass species. The current database provides the most up-to-date draft genome sequence along with structural and functional annotations for genes that can be accessed using Genome Browser (GBrowse), along with comparative genomic alignments to Arabidopsis, L. perenne, barley, rice, Brachypodium and maize genomes. We have integrated homologous search tool BLAST also for the users to analyze their data. Combined, GBrowse, BLAST and downloadable data gives a user-friendly access to meadow fescue genomic resources. To our knowledge, ForageGrassBase is the first genome database dedicated to forage grasses. The current forage grass database provides valuable resources for a range of research fields related to meadow fescue and other forage crop species, as well as for plant research communities in general. The genome database can be accessed at http://foragegrass.org.

scholarly journals ForageGrassBase: Molecular resource for the forage grass Festuca pratensis Huds

2019 ◽  
Author(s):  
Jeevan Karloss Antony Samy ◽  
Odd Arne Rognli ◽  
Mallikarjuna Rao Kovi
Keyword(s):  
Draft Genome ◽  
Forage Yield ◽  
Grass Species ◽  
Comparative Genomic ◽  
Festuca Pratensis ◽  
Forage Grass ◽  
Forage Grasses ◽  
Genome Database ◽  
Genomic Resources ◽  
Link Type

AbstractBackgroundMeadow fescue (Festuca pratensis Huds.) is one of the most important forage grasses in temperate regions. F. pratensis is a diploid (2n =14) outbreeding species that belongs to the genus Festuca. Together with Lolium, they are the most important genera of forage grasses in temperate regions. F. pratensis has good winter survival, with high quality dry matter yields and persistency, and is suitable both for frequent-cutting conservation regimes and for grazing. It is a significant component of species-rich permanent pastures in the temperate regions, ensuring high forage yield under harsh climatic conditions where other productive forage grass species are unable to grow. However, genomic resources for F. Pratensis is not available so far.ResultsThe draft genome sequences of two F. pratensis genotypes “HF7/2” and “B14/16” are reported in this study. Here, using the draft genome, functional annotation datasets of two F. pratensis cultivars, we have constructed the F. pratensis genome database http://foragegrass.org/, the first open-access platform to provide comprehensive genomic resources related to this forage grass species. The current version of this database provides the most up-to-date draft genome sequence along with structural and functional annotations for genes using Genome Browser (GBrowse). In addition, we have integrated comparative genomic tracks for F. pratensis genomes by mapping F.pratensis genome to the barley, rice, Brachypodium and maize genomes. We have integrated homologus search tool BLAST also for the users to analyze their data. Combined, GBrowse, BLAST and downloadble data gives an user friendly access to F. pratensis genomic resouces. All data in the database were manually curated.ConclusionTo our knowledge, ForageGrassBase is the first genome database dedicated to forage grasses. It provides valuable resources for a range of research fields related to F. pratensis and other forage crop species, as well as for plant research communities in general. The genome database can be accessed at http://foragegrass.org. In the near future, we will expand the ForageGrassBase by adding genomic tools for other forage grass species, as soon as their genomes become available.

Use of tall fescue EST-SSR markers in phylogenetic analysis of cool-season forage grasses

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 637-647 ◽  
Author(s):  
M A. Rouf Mian ◽  
Malay C Saha ◽  
Andrew A Hopkins ◽  
Zeng-Yu Wang
Keyword(s):  
Phylogenetic Analysis ◽  
Ssr Markers ◽  
Tall Fescue ◽  
Related Species ◽  
Grass Species ◽  
Forage Grass ◽  
Forage Grasses ◽  
Cool Season ◽  
Phylogenetic Relations ◽  
Wide Range

Microsatellites or simple sequence repeats (SSRs) are highly useful molecular markers for plant improvement. Expressed sequence tag (EST)-SSR markers have a higher rate of transferability across species than genomic SSR markers and are thus well suited for application in cross-species phylogenetic studies. Our objectives were to examine the amplification of tall fescue EST-SSR markers in 12 grass species representing 8 genera of 4 tribes from 2 subfamilies of Poaceae and the applicability of these markers for phylogenetic analysis of grass species. About 43% of the 145 EST-SSR primer pairs produced PCR bands in all 12 grass species and had high levels of polymorphism in all forage grasses studied. Thus, these markers will be useful in a variety of forage grass species, including the ones tested in this study. SSR marker data were useful in grouping genotypes within each species. Lolium temulentum, a potential model species for cool-season forage grasses, showed a close relation with the major Festuca–Lolium species in the study. Tall wheatgrass was found to be closely related to hexaploid wheat, thereby confirming the known taxonomic relations between these species. While clustering of closely related species was found, the effectiveness of such data in evaluating distantly related species needs further investigations. The phylogenetic trees based on DNA sequences of selected SSR bands were in agreement with the phylogenetic relations based on length polymorphism of SSRs markers. Tall fescue EST-SSR markers depicted phylogenetic relations among a wide range of cool-season forage grass species and thus are an important resource for researchers working with such grass species.Key words: phylogeny, EST-SSR, forage grasses, tall fescue.

scholarly journals Application of SCoT markers for accessing of genetic diversity of gramineous forage grass species

2021 ◽  
Vol 901 (1) ◽  
pp. 012038
Author(s):  
Yu M Mavlyutov ◽  
A O Shamustakimova ◽  
I A Klimenko
Keyword(s):  
Lolium Multiflorum ◽  
Molecular Genetic ◽  
Grass Species ◽  
Festuca Rubra ◽  
Festuca Pratensis ◽  
Forage Grass ◽  
Marker System ◽  
Scot Marker ◽  
Dna Profiles ◽  
Subsequent Identification

Abstract Using the SCoT marker system, 8 varieties of cereal grasses belonging to 5 species were analyzed: Festuca pratensis, Lolium perenne, Lolium multiflorum, Festuca rubra, Festulolium. Of the 10 tested SCoT markers, 7 informative markers were selected that reveal interspecies genetic polymorphism. According to the results of the analysis, DNA profiles characteristic of each studied species were obtained, and primers allowing to detect intervarietal differences for subsequent identification and molecular genetic passportization were selected.

Wind-mediated seed dispersal of invasive forage grasses from agricultural grasslands in Hokkaido, Japan

2019 ◽  
Vol 12 (02) ◽  
pp. 133-141
Author(s):  
Chika Egawa ◽  
Atsushi Shoji ◽  
Hiroyuki Shibaike
Keyword(s):  
Seed Dispersal ◽  
Poa Pratensis ◽  
Plant Cover ◽  
Kentucky Bluegrass ◽  
Dispersal Distance ◽  
Phleum Pratense ◽  
Grass Species ◽  
Forage Grass ◽  
Forage Grasses ◽  
Number Of Seeds

AbstractAlthough introduced pasture grasses are essential for forage production in current livestock farming, some species cause serious impacts on native biodiversity when naturalized. Information on the seed dispersal of invasive forage grasses from cultivated settings to surrounding environments can inform management efforts to prevent their naturalization. In this case study, we quantified the wind-mediated seed dispersal distance and amount of dispersed seed of invasive forage grasses from agricultural grasslands in Hokkaido, northern Japan. In total, 200 funnel seed traps were installed around three regularly mown grasslands and one unmown grassland where various forage grass species were grown in mixture. Seeds of each species dispersed outside the grasslands were captured from May to October 2017. Based on the trapped distances of seeds, the 99th percentile dispersal distance from the grasslands was estimated for six species, including timothy (Phleum pratense L.), orchardgrass (Dactylis glomerata L.), and Kentucky bluegrass (Poa pratensis L.). For two dominant species, P. pratense and D. glomerata, the numbers of seeds dispersed outside the field under mown and unmown conditions were determined under various plant cover situations. The estimated dispersal distances ranged from 2.3 m (P. pratense) to 31.5 m (P. pratensis), suggesting that areas within approximately 32 m of the grasslands are exposed to the invasion risk of some forage grass species. For both P. pratense and D. glomerata, the number of seeds dispersed outside the unmown grassland exceeded 100 seeds m−2 under high plant cover situations, while the number of seeds dispersed from the mown grasslands at the same plant cover level was less than one-third of that number. The results suggest that local land managers focus their efforts on frequent mowing of grasslands and monitoring of the areas within approximately 32 m of the grasslands to substantially reduce the naturalization of invasive forage grasses.

Growth and Nutrient Uptake of Orchardgrass (Dactylis glomerata L.) and Meadow Fescue (Festuca pratensis Huds.) as Affected by Rhizobacteria

2016 ◽  
Vol 44 (1) ◽  
pp. 296-301 ◽  
Author(s):  
Olivera STAJKOVIĆ-SRBINOVIĆ ◽  
Dušica DELIĆ ◽  
Djordje KUZMANOVIĆ ◽  
Biljana SIKIRIĆ ◽  
Nataša RASULIĆ ◽  
...  
Keyword(s):  
Plant Growth ◽  
Dactylis Glomerata ◽  
Grass Species ◽  
Festuca Pratensis ◽  
Meadow Fescue ◽  
Total N ◽  
Plant Growth Promoting ◽  
Uninoculated Control ◽  
Growth Promoting ◽  
Dactylis Glomerata L

A diverse group of soil bacteria found in the rhizosphere which can colonize plant roots and improve plant growth are designated as plant growth promoting rhizobacteria. The aim of this study was isolation and screening of different rhizobacterial strains for plant growth promoting characteristics and their ability to improve growth of two grass species, orchardgrass (Dactylis glomerata L.) and meadow fescue (Festuca pratensis Huds.). The strains investigated, belonging to the genera Azotobacter, Bacillus, Pseudomonas and rhizobial bacteria, showed various plant growth promoting traits, such as phosphate solubilisation, siderophore production, and indole-3-acetic acid (IAA) production. Co-inoculation of meadow fescue with Azotobacter chroococcum A2 and Sinorhizobium meliloti or Pseudomonas sp., and A. chroococcum A5 with S. meliloti, significantly increased shoot dry weight (SDW)(25-33%), as well as total N (26-33%), P (24-31%) and K (26-28%) contents in plants (mg pot-1), compared to uninoculated control. In addition, inoculation of orchardgrass with A. chroococcum strain A1, as well as co-inoculation with B. megaterium and A. chroococcum A1 or A31, significantly increased SDW (51-59%) and total N (54-59%), P (51-74%) and K (49-55%) contents, compared to uninoculated control. Nitrogen percentage in SDW was slightly higher than sufficiency ranges, while K percentage was optimal in all treatments in both species. Phosphorous percentage was lower than sufficiency ranges as a consequence of very low soil P content. The results emphasize the potential of particular rhizobacteria to improve the growth of forage grasses.

Repetitive DNA: A Versatile Tool for Karyotyping in Festuca pratensis Huds.

2017 ◽  
Vol 151 (2) ◽  
pp. 96-105 ◽  
Author(s):  
Anna Křivánková ◽  
David Kopecký ◽  
Štěpán Stočes ◽  
Jaroslav Doležel ◽  
Eva Hřibová
Keyword(s):  
Structural Changes ◽  
Tandem Repeats ◽  
Molecular Organization ◽  
Rrna Genes ◽  
Grass Species ◽  
Similar Distribution ◽  
Festuca Pratensis ◽  
Dna Repeats ◽  
Meadow Fescue ◽  
Molecular Karyotype

FISH is a useful method to identify individual chromosomes in a karyotype and to discover their structural changes accompanying genome evolution and speciation. DNA probes for FISH should be chromosome specific and/or exhibit specific patterns of distribution along each chromosome. Such probes are not available in many plants including meadow fescue (Festuca pratensis Huds.), an important forage grass species. In the present study, various DNA repeats identified in Illumina shotgun sequences specific to chromosome 4F of F. pratensis were used as probes for FISH to develop the molecular karyotype of meadow fescue and to reveal a long-range molecular organization of its chromosomes. Five tandem repeats produced specific patterns on individual chromosomes. Their use in combination with probes for rRNA genes enabled the establishment of the molecular karyotype of meadow fescue. Most of the mobile genetic elements were dispersed along all the chromosomes except for the DNA transposon CACTA, which was localized preferentially to telomeric and subtelomeric regions, and a putative LTR element, which was localized to (peri)centromeric regions. Cytogenetic mapping of the 5 tandem repeats in other accessions of meadow fescue showed a highly similar distribution and confirmed the versatility and robustness of these probes.

Comparison of meadow fescue and meadow bromegrass in monoculture and in association with white clover

2009 ◽  
Vol 89 (6) ◽  
pp. 1059-1063 ◽  
Author(s):  
R Drapeau ◽  
G Bélanger
Keyword(s):  
Key Words ◽  
White Clover ◽  
Trifolium Repens ◽  
Grass Species ◽  
Growing Degree Days ◽  
Festuca Pratensis ◽  
Degree Days ◽  
Meadow Fescue ◽  
Bromus Riparius ◽  
Meadow Bromegrass

Meadow fescue and meadow bromegrass alone or with white clover were compared in a four cut system in a region with less than 1400 growing degree days. Cultivars of the two grass species did not affect yield. The annual yields of two grass species, alone or with white clover, were similar in the first production year, but in second and third production years, meadow bromegrass yielded more than meadow fescue. The persistence of white clover, alone or with a grass, was limited. Key words: Festuca pratensis, Bromus riparius, Trifolium repens, yield

COLD HARDINESS OF FORAGE GRASSES GROWN ON THE CANADIAN PRAIRIES

1987 ◽  
Vol 67 (4) ◽  
pp. 1111-1115 ◽  
Author(s):  
A. E. LIMIN ◽  
D. B. FOWLER
Keyword(s):  
Triticum Aestivum ◽  
Cold Hardiness ◽  
Triticum Aestivum L ◽  
Grass Species ◽  
Forage Grass ◽  
Forage Grasses ◽  
Canadian Prairies ◽  
Seeding Date ◽  
Secale Cereale L ◽  
Cold Hardy

Cold hardiness ratings of 18 forage grass species, and cold hardy reference cultivars of winter wheat (Triticum aestivum L. ’Norstar’) and rye (Secale cereale L. ’Puma’), were compared to provide estimates of the winterkill risk for forage grasses established in the spring and fall on the Canadian prairies.Key words: Forage grasses, cold hardiness, seeding date, winter survival

A Demonstration of a 1:1 Correspondence Between Chiasma Frequency and Recombination Using a Lolium perenne/Festuca pratensis Substitution

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 307-314 ◽  
Author(s):  
J King ◽  
L A Roberts ◽  
M J Kearsey ◽  
H M Thomas ◽  
R N Jones ◽  
...  
Keyword(s):  
Lolium Perenne ◽  
Chiasma Frequency ◽  
Chiasma Formation ◽  
Grass Species ◽  
Festuca Pratensis ◽  
Single Chromosome ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Frequency Counts

Abstract A single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line (2n = 2x = 14). The chromatin of F. pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres.

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