scholarly journals P285 Is faecal immunochemical test a suitable alternative to faecal calprotectin in children?

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S294-S295
Author(s):  
K SANDHU ◽  
R Ayling ◽  
S Naik
Keyword(s):  
Intestinal Inflammation ◽  
Cost Effective ◽  
Paediatric Population ◽  
Diagnostic Value ◽  
Indeterminate Colitis ◽  
Faecal Calprotectin ◽  
Suitable Alternative ◽  
Faecal Immunochemical Test ◽  
Paired Samples ◽  
Immunochemical Test

Abstract Background Faecal calprotectin (FCP) has widely been used as a non-invasive marker for intestinal inflammation in children. Faecal immunochemical test (FIT) is well established in bowel cancer screening programmes in adults. FIT is cost-effective and easier to handle in comparison to FCP. We aimed to evaluate the performance of FIT in the paediatric population and compare it with FCP. Methods Clinicians in paediatric gastroenterology clinic who requested FCP for further investigation. These patients provided a sample of FIT from the same stool. These samples were collected over a 10-month period from November 2018 to September 2019. FIT samples were taken into a proprietary tube (Eiken Chemical, Tokyo, Japan) and stored at 4°C until analysis. FCP was measured using Liason Calprotectin (Diasorin, Italy). Results 131 samples were returned; 131 FIT and 102 FCP of which 7 calprotectin samples were insufficient for analysis. In 95 patients we had paired samples for FIT and FCP. The normal range for FCP was 0–200 µg/g and for FIT was 0–4 µg/g. Twenty-three of 95 patients were non-IBD (24.2%). In the IBD group; 42 had Crohn’s, 27 ulcerative colitis and 3 indeterminate colitis. 15 were new diagnosis of IBD. In the 95 patients; FIT was normal (<4 µg/g) in 50 patients and abnormal (>4 µg/g) in 45 patients. FCP was normal (<200 µg/g) in 45 patients and abnormal (>200 µg/g) in 50 patients. FIT positively correlated with calprotectin, Spearman’s rank coefficient 0.653, p < 0.001. There were 32 patients with FIT >20µg/g and in 29 of these patients, FCP was >200 µg/g. In 60 patients with FIT and in 35 patients with FCP underwent colonoscopies. Table 1 shows the diagnostic value of FIT in comparison to histological inflammation. The correlation of FIT with histological findings are shown in Figure 1. All patients with normal histology had a FIT <4 µg/g. In 91.4% of patients with moderate to severe histological inflammation had a FIT >5 µg/g. Conclusion Our study is the first to compare FIT and FCP in the paediatric population. Our results suggest that FIT correlates well with FCP and can be used to differentiate between functional bowel disease and inflammation in children. A FIT of > 20 µg/g was consistent with the finding of severe inflammation.

Use of faecal immunochemical testing as an alternative to faecal calprotectin in children

2021 ◽  
pp. 000456322198935
Author(s):  
Kirn Sandhu ◽  
Sandhia Naik ◽  
Ruth M Ayling
Keyword(s):  
Bowel Disease ◽  
Intestinal Inflammation ◽  
Paediatric Population ◽  
Mucosal Healing ◽  
Faecal Calprotectin ◽  
Non Invasive ◽  
Three Samples ◽  
Immunochemical Test ◽  
Inflammatory Bowel ◽  
Rule Out

Background Faecal calprotectin has been widely used as a non-invasive marker of intestinal inflammation in children. Measurement of faecal haemoglobin using faecal immunochemical test is well established in adults for detection of colorectal cancer. In adults, faecal haemoglobin has been recommended as a reliable tool to aid identification of those at low risk of significant bowel disease and has also been used in inflammatory bowel disease to assess mucosal healing. Aims We aimed to evaluate the performance of faecal haemoglobin in the paediatric population and compare it with faecal calprotectin. Methods Children being assessed in the paediatric gastroenterology clinic for bowel symptoms had a sample sent for both faecal calprotectin and faecal haemoglobin. Samples were collected over a 10-month period from November 2018 to September 2019. Faecal haemoglobin was measured using an OC-Sensor. Faecal calprotectin was measured using Liason®Calprotectin. Results One hundred forty three samples were returned for faecal haemoglobin and in 107 a paired faecal calprotectin was also available. Faecal haemoglobin correlated with faecal calprotectin, Spearman’s rank coefficient 0.656 ( P < 0.0001). There were 35 patients with faecal haemoglobin >20 μg/g and in 32 of these patients faecal calprotectin was >200 μg/g; 74 patients with faecal haemoglobin and 38 patients with faecal calprotectin underwent colonoscopy. Patients with normal histology had faecal haemoglobin <4 μg/g; faecal haemoglobin >20 µg/g was associated with signification inflammation Conclusion Our study is the first to compare faecal haemoglobin and faecal calprotectin in a paediatric population. Results suggest that faecal haemoglobin correlates with faecal calprotectin and, as in adults, may be useful to rule out significant bowel disease. A faecal haemoglobin >20 μg/g was consistent with significant histological inflammation.

scholarly journals Guidance on the interpretation of faecal calprotectin levels in children

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246091
Author(s):  
Martina Orfei ◽  
Marco Gasparetto ◽  
Kai O. Hensel ◽  
Florian Zellweger ◽  
Robert B. Heuschkel ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Catchment Area ◽  
Gastrointestinal Symptoms ◽  
Reference Value ◽  
Cost Effective ◽  
Diagnostic Value ◽  
Older Children ◽  
Faecal Calprotectin ◽  
Blood Tests ◽  
Inflammatory Bowel

Background Faecal calprotectin (FCP) is a powerful tool to predict inflammatory bowel disease (IBD) in patients with gastrointestinal symptoms. In the paediatric patient population, the reference value of < 50 μg/g and the influence of age on FCP levels result in a high number of redundant investigations and specialist referrals. We assessed paediatric FCP levels, their diagnostic value and corresponding referral pathways from primary and secondary care. Methods We analysed two cohorts from a precisely defined catchment area: one consisted of all FCPs measured in this area (n = 2788). The second cohort—a subset of the first cohort—consisted of FCP values and corresponding clinical data from children who were referred for possible IBD to our department (n = 373). Results In the first cohort, 47% of FCP levels were > 50 μg/g, 15% were ≥ 250 μg/g. Children < 1y had significantly (p < 0.001) higher FCP than older children. In the second cohort, 6.7% of children with an FCP of < 250 μg/g (or 8.6% with an FCP of < 600 μg/g) had IBD–all featured symptoms suggestive of IBD (e.g. bloody diarrhoea, nocturnal abdominal pain, weight loss) or abnormal blood tests. 76% of patients in whom raised FCP (> 50 μg/g) was the sole reason for being referred for suspected IBD did not have IBD. Conclusion Children with an FCP < 600 μg/g and without matching symptoms suggestive of IBD are unlikely to have IBD. A higher FCP reference value may provide cost-effective improvement that could avoid redundant investigations and specialist referrals. A guideline for specialist referrals is proposed.

scholarly journals Quantitation of faecalFusobacteriumimproves faecal immunochemical test in detecting advanced colorectal neoplasia

Gut ◽  
2016 ◽  
Vol 66 (8) ◽  
pp. 1441-1448 ◽  
Author(s):  
Sunny H Wong ◽  
Thomas N Y Kwong ◽  
Tai-Cheong Chow ◽  
Arthur K C Luk ◽  
Rudin Z W Dai ◽  
...  
Keyword(s):  
Colorectal Neoplasia ◽  
Faecal Immunochemical Test ◽  
Advanced Colorectal Neoplasia ◽  
Immunochemical Test

scholarly journals PSVII-3 Correlations between L-lactate concentrations obtained using a handheld lactate analyser and a lactate assay colorimetric kit in beef cattle transported by road

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 296-297
Author(s):  
Daniela M Meléndez ◽  
Sonia Marti ◽  
Luigi Faucitano ◽  
Derek B Haley ◽  
Timothy D Schwinghamer ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Cost Effective ◽  
Road Transportation ◽  
Long Distance ◽  
Suitable Alternative ◽  
Blood Samples ◽  
Handheld Device ◽  
Time Points ◽  
Relationship Of ◽  
The Relationship

Abstract Blood metabolites are used to assess a variety of animal conditions for veterinary diagnosis and research. Concentration of metabolites in blood can be measured using a commercially-available lab-based assay or in real-time using a handheld device developed to be more time- and cost-effective than the lab-based method. Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer (Lactate Scout, EFK Diagnostics, Barleben, Germany) and a lactate assay colorimetric kit (Lactate Assay Kit, Cell Biolabs Inc., San Diego, CA). Blood samples were collected by venipuncture from 96 steers (245 ± 35.7 kg BW) prior to (L1) and after 36 h, and prior to and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in colorimetric analysis. Pearson correlations were calculated to determine the relationship between the experimental methods for the quantification of L-lactate concentrations. The strengths and levels of statistical significance of the correlation varied over the observed time points, r = -0.03, P = 0.75 (L1) to r = 0.75, P = &lt; 0.0001 (d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, P &lt; 0.001). Based on the experimental results, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay for measuring L-lactate in transported cattle, due to variability across sampling time points and weak correlation with the traditional enzymatic method.

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