scholarly journals PSVII-3 Correlations between L-lactate concentrations obtained using a handheld lactate analyser and a lactate assay colorimetric kit in beef cattle transported by road

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 296-297
Author(s):  
Daniela M Meléndez ◽  
Sonia Marti ◽  
Luigi Faucitano ◽  
Derek B Haley ◽  
Timothy D Schwinghamer ◽  
...  

Abstract Blood metabolites are used to assess a variety of animal conditions for veterinary diagnosis and research. Concentration of metabolites in blood can be measured using a commercially-available lab-based assay or in real-time using a handheld device developed to be more time- and cost-effective than the lab-based method. Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer (Lactate Scout, EFK Diagnostics, Barleben, Germany) and a lactate assay colorimetric kit (Lactate Assay Kit, Cell Biolabs Inc., San Diego, CA). Blood samples were collected by venipuncture from 96 steers (245 ± 35.7 kg BW) prior to (L1) and after 36 h, and prior to and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in colorimetric analysis. Pearson correlations were calculated to determine the relationship between the experimental methods for the quantification of L-lactate concentrations. The strengths and levels of statistical significance of the correlation varied over the observed time points, r = -0.03, P = 0.75 (L1) to r = 0.75, P = < 0.0001 (d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, P < 0.001). Based on the experimental results, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay for measuring L-lactate in transported cattle, due to variability across sampling time points and weak correlation with the traditional enzymatic method.

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 926
Author(s):  
Daniela M. Meléndez ◽  
Sonia Marti ◽  
Luigi Faucitano ◽  
Derek B. Haley ◽  
Timothy D. Schwinghamer ◽  
...  

Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected by venipuncture from 96 steers (Black or Red Angus × Hereford/Simmental and Black or Red Angus × Charolais; 247 ± 38.2 kg BW) prior to loading (LO1) and after 36 h of transport, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Pearson correlations were calculated to assess the strength of the relationship between the experimental methods for the quantification of L-lactate concentrations. The magnitude and direction of the correlation, and the level of statistical significance varied over the observed time points, ranging from r = −0.03 (p = 0.75; LO1) to r = 0.75 (p < 0.0001; d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, p < 0.0001). Based on the low magnitude of the correlation due to variability across sampling time points in this study, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay (considered the gold standard) for measuring L-lactate in transported cattle.


2018 ◽  
Vol 23 ◽  
pp. 2515690X1880477
Author(s):  
Suzanne Reed ◽  
Kathi J. Kemper ◽  
Alan Schwartz ◽  
Maneesh Batra ◽  
Betty B. Staples ◽  
...  

Residency is a high-risk period for physician burnout. We aimed to determine the short-term stability of factors associated with burnout, application of these data to previous conceptual models, and the relationship of these factors over 3 months. Physician wellness questionnaire results were analyzed at 2 time points 3 months apart. Associations among variables within and across time points were analyzed. Logistic regression was used to predict burnout and compassionate care. A total of 74% of residents completed surveys. Over 3 months, burnout ( P = .005) and empathy ( P = .04) worsened. The most significant cross-sectional relationship was between stress and emotional exhaustion (time 1 r = 0.61, time 2 r = 0.68). Resilience was predictive of increased compassionate care and decreased burnout ( P < .05). Mindfulness was predictive of decreased burnout ( P < .05). Mitigating stress and fostering mindfulness and resilience longitudinally may be key areas of focus for improved wellness in pediatric residents. Larger studies are needed to better develop targeted wellness interventions.


2016 ◽  
Vol 12 (2) ◽  
pp. 75-82 ◽  
Author(s):  
C. Berkman ◽  
M.C. Pereira ◽  
K.B. Nardi ◽  
G.T. Pereira ◽  
O.A.B. Soares ◽  
...  

Little information is available comparing the i-STAT and the YSI 2300 Stat Plus devices to determine the lactate concentration [Lac] in dogs undergoing intense exercise. The reproducibility of the YSI 2300 for quantifying the [Lac] in canine blood [Lac]b and plasma [Lac]p samples has been observed. In addition, the i-STAT handheld device was used to quantify [Lac] in dogs subjected to exercise, and the results were compared with that of YSI 2300. Venous blood samples of Beagle and American Pit Bull Terrier dogs were obtained during an intense exercise training on a treadmill. [Lac]p and [Lac]b were quantified using the YSI 2300 instrument to determine the reproducibility of the results. A total of 52 specimens were compared for both plasma and whole blood. For comparing the devices (YSI 2300 vs i-STAT), 96 samples were used. Ordinary least products regression, the correlation coefficient, and Bland-Altman plots were used to assess the agreement of using the i-STAT device. The relationship between duplicate measurements of both [Lac]b and [Lac]p by YSI 2300 was strong (r=0.99). A correlation between the data obtained using the i-STAT and YSI 2300 instruments was observed for both the [Lac]p (r=0.97) and [Lac]b (r=0.88). The i-STAT exhibited a small constant bias (-0.25 mmol/l) compared to YSI 2300 ([Lac]b). There were proportional biases of 0.89 mmol/l for [Lac]p and 1.22 mmol/l for [Lac]b when using YSI 2300 vs i-STAT. We confirmed the reproducibility of the YSI 2300 for canine lactate blood/plasma samples. The results obtained by the i-STAT and YSI 2300 analyser were highly correlated, but a small constant bias was observed between them. The i-STAT device can be used in clinical evaluations, and it is also adequate for designing and monitoring fitness programmes.


AL-HUKAMA ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 359-479
Author(s):  
Maulidia Mulyani

In the present time the impact of technological progress can be felt in many aspects of life, including marriage. The progress of the development of communication technology has made it easy to communicate specifically for married couples who are undergoing long-distance marital relationships, so that the relationship is maintained and harmonious. Social media is now used as a tool for communication, for example WhatsApp, Line, Facebook, or Instagram. All of those media are used because of their speed in conveying messages, so that long-distance couples can still communicate well and quickly. In a marital relationship, of course, there are fulfillments of rights and obligations. These fulfillments can still be fulfilled through social media, but they are not as maximized as when dealing directly.


2000 ◽  
Vol 44 (12) ◽  
pp. 2-503-2-506 ◽  
Author(s):  
Robert L. Getty

The human element pervades all company processes, from proposal, design, manufacturing, quality control and product support. Cost effective processes can be best achieved when the human element is totally integrated with technology. Traditional human engineering design methodologies applied to company processes optimizes the relationship of people that comprise the organization with the technology that is the foundation of the organization. Process ownership is essential for the achievement of the goals of quality. This ownership occurs by applying macroergonomics precepts by integration of the personnel system with the technological factors to achieve product delivery to a satisfied customer within the external environment of market forces. The application of human engineering design principles will be discussed followed by the quality focus of the LMTAS company processes. This paper will review LMTAS quality goals to show the elements that form the foundation of The Fighter Enterprise to make it the contractor of choice for tactical fighter aircraft.


2021 ◽  
pp. 73-78
Author(s):  
Roy Romey Mangunsong ◽  
Sudarman Sudarman

Background: The occurrence of serious problems in children's mental development, that children will become aggressive, lack of social interaction and deteriorating of communication skills, is affected by the introduction of gadgets too early and the intensity of uncontrolled use of gadgets by parents. Aim of this study is to determine the relationship between pragmatic ability and social interaction with the intensity of using gadgets in kindergarten children in Surakarta.  Methods: This research was conducted in the kindergarden TK ABA Thoyibah Surakarta, TK Sri Juwita Hanum Surakarta dan TK Negeri Pembina city of Surakarta in april until august 2020. Sample conduct using total sampling technique. Amount of sample is 115 students of kindergarden. The relationship of the variables studied, both pragmatic ability and social interaction with the intensity of using gadgets, is an Odds Ratio (OR), which is exponential from b, the statistical significance of the Odds Ratio is tested with mannova.  Results: The results of the analysis of the pragmatic ability variable showed that Exp B: 2,916, p: 0,420. The results of the social interaction variable analysis showed that Exp B: 2,977, p: 0.011.  Conclusion: Pragmatic ability and social intensity are jointly related to the intensity of using gadgest in kindergarten students in Surakarta, with the results of the analysis of the variable pragmatic ability Exp B: 2,916, p: 0,420 and the results of the analysis of social interaction variables Exp B: 2,977, p: 0.011.


2020 ◽  
Author(s):  
Dimitar Valev

AbstractThe statistical relationships of total COVID-19 Cases and Deaths per million populations in 45 countries, where 85.8% of the world’s population lives with 10 demographic, economic and social indicators were studied. Data for 28 May 2020 were used in the main calculations. The relationship of Deaths per million population and total Cases per million population is very close and reaches correlation coefficient R = 0.926. It is interesting that the close correlations were found of Cases and Deaths per 1 million with a purely economic index like GDP PPP per capita, where R = 0.687 and R = 0.660, respectively. Even more close correlations were found of Cases and Deaths per 1 million with a composite index HDI, where the correlation coefficients reach 0.724 and 0.680, respectively. The main reason for these paradoxical results is the underestimation of pandemic restrictions in the form of masks, social distance and disinfection in most of these countries. Other indicators (excluding Gini index and Population Density) also show statistically significant correlations with Cases and Deaths per 1 million with correlation coefficients from 0.432 to 0.634. The statistical significance of the found correlations determined using Student’s t-test was p <0.0001. Surprisingly, there was no statistically significant correlation between Cases and Deaths with Population Density. To check whether there is a change in the correlations with the development of the pandemic, a statistical analysis was made for four different dates – 9 April, 28 May, 7 August and 30 November 2020. It was found that the correlation coefficients of COVID-19 cases and Deaths with the rest indicators decrease during the pandemic.


2020 ◽  
Vol 7 (4) ◽  
pp. 191700 ◽  
Author(s):  
Carlos Buenaventura Castillo ◽  
Andy G. Lynch ◽  
Silvia Paracchini

The most common way to assess handedness is based on the preferred hand for writing, leading to a binary (left or right) trait. Handedness can also be assessed as a continuous trait with laterality indexes, but these are not time- and cost-effective, and are not routinely collected. Rarely, different handedness measures are collected for the same individuals. Here, we assessed the relationship of preferred hand for writing with four laterality indexes, reported in previous literature, derived from measures of dexterity (pegboard task, marking squares and sorting matches) and strength (grip strength), available in a range of N = 6664–8069 children from the ALSPAC cohort. Although all indexes identified a higher proportion of individuals performing better with their right hand, they showed low correlation with each other (0.08–0.3). Left handers were less consistent compared to right handers in performing better with their dominant hand, but that varied across indexes, i.e. 13% of left handers performed better with their right hand on marking squares compared to 48% for sorting matches and grip strength. Analysis of sex effects on the laterality indexes showed that males and females tend to be, on all measures, more left- and right-lateralized, respectively. Males were also over-represented among the individuals performing equally with both hands suggesting they had a higher tendency to be weakly lateralized. This study shows that different handedness measures tap into different dimensions of laterality and cannot be used interchangeably. The trends observed across indexes for males and females suggest that sex effects should be taken into account in handedness and laterality studies.


2018 ◽  
Vol 59 (1) ◽  
pp. 120-139 ◽  
Author(s):  
Yoojin Shin ◽  
Breffni M. Noone ◽  
Stephani K. A. Robson

This research employed two experimental studies to examine the relationship of photograph content with consumers’ online travel booking intentions, the role of photograph source in this relationship, and the impact of photograph content on consumer reaction to price. Study 1’s findings suggest that perceived information value mediates the effect of photograph content on consumers’ booking intentions, with product-focused photographs perceived as being higher in information value than experience-focused photographs. Further, perceived photograph credibility was found to influence consumer reaction to photograph content, with photograph source moderating the photograph content-perceived photograph credibility relationship. Study 2’s findings suggest that photograph content moderates the price–booking intentions relationship. When the price is higher than the average in the market, consumers are likely to rely more heavily on product-focused photographs than on experience-focused photographs to inform the booking decision. Together, the findings of these studies can guide travel marketers in developing cost-effective, photograph-based online content.


Twin Research ◽  
2000 ◽  
Vol 3 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Michael J Lyons ◽  
Jonathan Huppert ◽  
Rosemary Toomey ◽  
Rebecca Harley ◽  
Jack Goldberg ◽  
...  

AbstractThere have been long questions about the relationship of schizophrenia to other mental disorders. Lifetime DSM-III-R diagnoses of mood and anxiety disorders in twins with clinically diagnosed schizophrenia (n = 24) and their non-affected co-twins (n = 24) were compared with twins from pairs without schizophrenia (n = 3327) using a sample from the Vietnam Era Twin Registry. Schizophrenic probands had significantly elevated rates of all included disorders (bipolar disorder, major depression, dysthymia, generalized anxiety disorder, panic disorder, and PTSD) compared with controls (P < 0.01). The odd ratios comparing co-twins of schizophrenic probands with controls was greater than three for every disorder, but did not attain statistical significance. A similar pattern was observed when analyses were restricted to only monozygotic twins (n = 12). Consistent with other studies, schizophrenics appeared to have higher rates of a range of mental disorders. Our results suggest that schizophrenia per se represents a risk factor for other psychiatric disorders, but the absence of significantly elevated risk among non-schizophrenic co-twins suggested that family environmental and/or genetic factors that contribute to risk of schizophrenia do not increase the risk of mood and anxiety disorders to the same extent that the risk of these other disorders is increased by the presence of schizophrenia. Twin Research (2000) 3, 28–32.


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