scholarly journals SEGMENTAL ANEUPLOIDY AND ENZYME ACTIVITY AS A METHOD FOR CYTOGENETIC LOCALIZATION IN DROSOPHILA MELANOGASTER

Genetics ◽  
1974 ◽  
Vol 76 (2) ◽  
pp. 301-309
Author(s):  
Barbara R Stewart ◽  
John R Merriam
Keyword(s):  
Enzyme Activity ◽  
X Chromosome ◽  
Gene Dosage ◽  
Chromosome Region ◽  
Structural Genes ◽  
The Third ◽  
Segmental Aneuploidy ◽  
Cytological Localization ◽  
The Relationship ◽  
Chromosome Bands

ABSTRACT A method of mapping genes which specify enzymes without the necessity of obtaining genetic variants has been explored. Three enzymes whose structural genes have known genetic positions were chosen to see if the relationship between gene dosage and enzyme activity could be used as a tool in cytological localization. Zw, the gene specifying G6PD, is located in the X chromosome region, 18D-18F. The structural gene for 6PGD, Pgd, maps in the X chromosome bands 2C1-2E1. Idh-NADP, the gene which specifies IDH-NADP, is found on the third chromosome, in bands 66B-67C.

scholarly journals SEGMENTAL ANEUPLOIDY AS A PROBE FOR STRUCTURAL GENES IN DROSOPHILA: MITOCHONDRIAL MEMBRANE ENZYMES

Genetics ◽  
1973 ◽  
Vol 75 (1) ◽  
pp. 155-167
Author(s):  
Stephen J O'Brien ◽  
Richard C Gethmann
Keyword(s):  
Mitochondrial Membrane ◽  
Gene Dosage ◽  
Succinic Dehydrogenase ◽  
Structural Genes ◽  
Malic Dehydrogenase ◽  
Autosomal Region ◽  
Glycerophosphate Dehydrogenase ◽  
Enzyme Systems ◽  
Segmental Aneuploidy ◽  
Chromosome Bands

ABSTRACT A method for detecting possible structural genes in D. melanogaster based on gene dosage dependency is presented. By making thirty crosses between Y-autosome translocations, and an attached-4 cross, it is possible to produce large duplications (approximately 150 salivary gland chromosome bands in length) for every autosomal region with the exception of 83DE. The usefulness of the technique was demonstrated by dosage dependency of three known gene-enzyme systems: α-glycerophosphate dehydrogenase-1, alcohol dehydrogenase and malate dehydrogenase. A screen for genes affecting two enzymes localized on the inner membrane of the mitochondrion, α-glycerophosphate oxidase (αGPO) and succinic dehydrogenase (SHD), produced a dosage-sensitive region in each case. Region 50C-52E affected αGPO activity and region 28D-29F affected SDH activity. The latter region apparently includes the malic dehydrogenase-1 gene. The methodology and limitations of the technique are discussed.

scholarly journals A CYTOGENETIC ANALYSIS OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE ACTIVITIES IN DROSOPHILA

Genetics ◽  
1977 ◽  
Vol 85 (4) ◽  
pp. 609-622
Author(s):  
John A Kiger ◽  
Eric Golanty
Keyword(s):  
Drosophila Melanogaster ◽  
Structural Gene ◽  
X Chromosome ◽  
Cyclic Nucleotide ◽  
Phosphodiesterase Activity ◽  
Structural Genes ◽  
Camp Phosphodiesterase ◽  
Cgmp Phosphodiesterase ◽  
The Third ◽  
Segmental Aneuploidy

ABSTRACT The genome of Drosophila melanogaster has been surveyed for chromosomal regions which exert a dosage effect on the activities of cAMP phosphodiesterase or cGMP phosphodiesterase. Two regions increase cAMP phosphodiesterase activity when present as duplications. A region of the X chromosome increases cAMP phosphodiesterase activity when duplicated and decreases that activity when deficient. This region has been delimited to chromomeres 3D3 and 3D4, with 3D4 being the most probable locus, and may contain a structural gene for cAMP phosphodiesterase. A region on the third chromosome, 90E-91B, increase cAMP phosphodiesterase activity when duplicated but has no affect on the activity when deficient. Two regions increase cGMP phosphodiesterase activity when present as duplications. A region of the X chromosome, 5D-9C, increases cGMP phosphodiesterase activity when duplicated, but smaller duplications covering this region fail to show such an increase, indicating that a single locus is not responsible for the increase observed for the larger duplication. A region of the third chromosome, 88C-91B, also increases cGMP phosphodiesterase activity when duplicated. Smaller duplications covering this region show smaller increases than that observed for the larger duplication, suggesting that at least three loci between 88C and 91B contribute to the observed increase by that region. Deficiencies covering region 88C-91B do not affect cGMP phosphodiesterase activity. No locus for a presumptive structural gene for cGMP phosphodiesterase has been found. Limitations of the use of segmental aneuploidy in locating structural genes for enzymes are discussed.

scholarly journals Suppression of a lethal trisomic phenotype in Drosophila melanogaster by increased dosage of an unlinked locus.

Genetics ◽  
1993 ◽  
Vol 134 (1) ◽  
pp. 243-249 ◽  
Author(s):  
D R Dorer ◽  
M A Cadden ◽  
B Gordesky-Gold ◽  
G Harries ◽  
A C Christensen
Keyword(s):  
Drosophila Melanogaster ◽  
X Chromosome ◽  
Gene Dosage ◽  
Chromosome Region ◽  
Dosage Effect ◽  
Gene Dosage Effect ◽  
Unlinked Locus ◽  
Lethal Phenotype ◽  
The Third ◽  
Dosage Sensitivity

Abstract One of the most extreme examples of gene dosage sensitivity is the Triplo-lethal locus (Tpl) on the third chromosome of Drosophila melanogaster, which is lethal when present in either one or three copies. Increased dosage of an unlinked locus, Isis, suppresses the triplo-lethal phenotype of Tpl, but not the haplo-lethal phenotype. We have mapped Isis to the X chromosome region 7E3-8A5, and shown that the suppression is a gene dosage effect. Altered dosage of Isis in the presence of two copies of Tpl has no obvious effects. By examining the interactions between Isis dosage and Tpl we suggest that Isis does not directly repress Tpl expression, but acts downstream on the triplo-lethal phenotype of Tpl.

scholarly journals DOSAGE COMPENSATION IN DROSOPHILA: NADP-ENZYME ACTIVITIES AND CROSS-REACTING MATERIAL

Genetics ◽  
1983 ◽  
Vol 103 (4) ◽  
pp. 649-658
Author(s):  
John H Williamson ◽  
Michael M Bentley
Keyword(s):  
Enzyme Activity ◽  
Structural Gene ◽  
X Chromosome ◽  
Dosage Compensation ◽  
Enzyme Activities ◽  
Malic Enzyme ◽  
Gene Dosage ◽  
G6pd Activity ◽  
Structural Genes ◽  
Malic Enzyme Activity

ABSTRACT The relationships between gene dosage, enzyme activities and CRM levels have been determined for G6PD and 6PGD. Enzyme activities and CRM levels were directly proportional and increased in genotypes carrying duplications of the respective structural genes. When a duplication consisting of the distal 45% of the X chromosome was used to duplicate Pgd  +, 6PGD activity and CRM increased and G6PD activity decreased. When the proximal 55% of the X chromosome was duplicated, G6PD activity and CRM increased whereas 6PGD activity and CRM levels decreased. These observations support the model of dosage compensation of X-linked genes that invokes an autosomal activator in limited concentrations for which X-linked loci compete. The distal 45% of the X chromosome, when duplicated, caused a significant increase in NADP-malic enzyme activity and CRM levels, as if a structural gene for NADP-ME is sex-linked.

scholarly journals Molecular and cytogenetical characterization of the 10A1-2 band and adjoining region in the Drosophila melanogaster polytene X chromosome.

Genetics ◽  
1994 ◽  
Vol 136 (3) ◽  
pp. 1063-1073 ◽  
Author(s):  
T u Kozlova ◽  
V F Semeshin ◽  
I V Tretyakova ◽  
E B Kokoza ◽  
V Pirrotta ◽  
...  
Keyword(s):  
X Chromosome ◽  
Physical Map ◽  
Chromosome Region ◽  
Chromosome Rearrangements ◽  
P Element ◽  
Induced Mutations ◽  
Physical Maps ◽  
Break Points ◽  
Chromosome Bands

Abstract Some 300 kb of DNA from the 9F12-10A7 X chromosome region (seven bands) uncovered by Df(1)vL3 were cloned and 31 break points of chromosome rearrangements within the region were mapped. Positions of 12 genes found earlier in genetic saturation experiments, transcripts and P element-induced mutations were located on the physical map using either chromosome rearrangements or Southern blot hybridizations. Data on the position of the break points, genes and polytene chromosome bands allow the following conclusions to be made. (1) The size of the bands in the region varies between 4 kb (10A6 and 7) and 183-195 kb (10A1-2). The compaction ratio of DNA in bands varies from 8-36 (10A6 + 7) to 151-161 (10A1-2). Therefore, fine and thick bands appear to have different kinds of DNP packaging. (2) The bands differ in genetic content. Fine bands contain from one to three genes. In contrast, the 10A1-2 band contains three genes and at least six transcribed DNA fragments. (3) Comparison of genetic and physical maps shows that in this region 0.01 centiMorgan corresponds to 3.3 kb of DNA.

scholarly journals Taste sensitivity to trehalose and its alteration by gene dosage in Drosophila melanogaster.

Genetics ◽  
1988 ◽  
Vol 119 (2) ◽  
pp. 399-406
Author(s):  
T Tanimura ◽  
K Isono ◽  
M T Yamamoto
Keyword(s):  
Drosophila Melanogaster ◽  
X Chromosome ◽  
Gene Dosage ◽  
Taste Sensitivity ◽  
Gene Dosage Effect ◽  
Upper Limit ◽  
Proportional Increase ◽  
Cytological Localization ◽  
Low Sensitivity ◽  
Normal Dosage

Abstract The taste sensitivity to the disaccharide trehalose of Drosophila melanogaster is under the genetic control by the Tre gene on the X chromosome. The gene is genetically dimorphic for high and low sensitivity and is likely to be functioning in the primary step of chemoreception. We have determined the cytological localization of the Tre gene to be between 5A10 and 5B1-3 by analyzing the sensitivity to trehalose in flies which are segmentally aneuploid bearing either deficiencies or duplicated fragments of T(X;Y) translocations. We also constructed flies which are aneuploidy and thus carry different dosage of Tre and/or Tre(+) alleles in order to examine the gene dosage effect on trehalose sensitivity and to deduce the nature of the gene's action. Trehalose sensitivity decreased in females carrying half the normal dosage of a given Tre allele, but a proportional increase in sensitivity was not observed in flies bearing a duplication of the Tre alleles. The changes in sensitivity in various aneuploid flies suggest that there is an upper limit to the number of molecules that can be incorporated into the receptor membrane. Genetic evidence strongly suggests that Tre is the structural gene for the trehalose receptor. We present a model to account for the mechanism of genetical control on the sensitivity to trehalose.

Retroaktive Neuverhandlung

2018 ◽  
Vol 63 (1) ◽  
Author(s):  
Daniel Martin Feige
Keyword(s):  
Original Work ◽  
The Other ◽  
Final Part ◽  
Critical Reading ◽  
The Third ◽  
Works Of Art ◽  
The Relationship

Der Beitrag widmet sich der Frage historischer Folgeverhältnisse in der Kunst. Gegenüber dem Gedanken, dass es ein ursprüngliches Werk in der Reihe von Werken gibt, das späteren Werken seinen Sinn gibt, schlägt der Text vor, das Verhältnis umgekehrt zu denken: Im Lichte späterer Werke wird der Sinn früherer Werke neu ausgehandelt. Dazu geht der Text in drei Schritten vor. Im ersten Teil formuliert er unter der Überschrift ›Form‹ in kritischer Abgrenzung zu Danto und Eco mit Adorno den Gedanken, dass Kunstwerke eigensinnig konstituierte Gegenstände sind. Die im Gedanken der Neuverhandlung früherer Werke im Lichte späterer Werke vorausgesetzte Unbestimmtheit des Sinns von Kunstwerken wird im zweiten Teil unter dem Schlagwort ›Zeitlichkeit‹ anhand des Paradigmas der Improvisation erörtert. Der dritte und letzte Teil wendet diese improvisatorische Logik unter dem Label ›Neuaushandlung‹ dann dezidiert auf das Verhältnis von Vorbild und Nachbild an. The article proposes a new understanding of historical succession in the realm of art. In contrast to the idea that there is an original work in the series of works that gives meaning to the works that come later, the text proposes to think it exactly the other way round: in the light of later works, the meanings of earlier works are renegotiated. The text proceeds in three steps to develop this idea. Under the heading ›Form‹ it develops in the first part a critical reading of Danto’s and Eco’s notion of the constitution of the artworks and argues with Adorno that each powerful work develops its own language. In the second part, the vagueness of the meaning of works of art presupposed in the idea of renegotiating earlier works in the light of later works is discussed under the term ›Temporality‹ in terms of the logic of improvisation. The third and final part uses this improvisational logic under the label ›Renegotiation‹ to understand the relationship between model and afterimage in the realm of art.

RECREATION SPECIALIZATION FOR RUNNERS AND EVENT ATTACHMENT

2020 ◽  
Author(s):  
Isao Okayasu ◽  
Chi-Ok Oh ◽  
Duarte B Morais
Keyword(s):  
Practical Implication ◽  
Event Management ◽  
Attitudes And Behaviors ◽  
The Third ◽  
Study Results ◽  
The World ◽  
Recreation Specialization ◽  
And Behaviors ◽  
The Relationship ◽  
Predictive Relationship

Running is one of the most popular activities in the world. Runners’ attitudes and behaviors vary depending on their running style. This study aims to construct different measures of running specialization based on the theory of specialization. This study also tests a runner’s stage of specialization segmentation based on recreation specialization and examines the predictive relationship between a runner’s specialization and event attachment. Three groups of sampling data assess the performance of diverse specialization measures for running in three marathon events. First, two surveys were conducted with marathon participants to assess the performance of diverse specialization measures for runners. Second, the third dataset was used to examine the relationship between a runner’s recreation specialization and event attachment.The study results showed that the 15 measures of specialization showed a good fit to the data. Our research showed how runners’ recreation specialization is connected to their event attachment. In addition, this study suggested event management for subdivisions of runners. Its practical implication is that recreation specialization for running can help us understand event attachment.

scholarly journals A Haplolethal Locus Uncovered by Deletions in the Mouse t Complex

Genetics ◽  
2002 ◽  
Vol 160 (2) ◽  
pp. 675-682
Author(s):  
Victoria L Browning ◽  
Rebecca A Bergstrom ◽  
Sandra Daigle ◽  
John C Schimenti
Keyword(s):  
Gene Dosage ◽  
Es Cells ◽  
Embryonic Stem ◽  
Chromosome 17 ◽  
Mammalian Development ◽  
T Complex ◽  
Segmental Aneuploidy ◽  
Systematic Exploration ◽  
Radiation Induced ◽  
Altered Gene

Abstract Proper levels of gene expression are important for normal mammalian development. Typically, altered gene dosage caused by karyotypic abnormalities results in embryonic lethality or birth defects. Segmental aneuploidy can be compatible with life but often results in contiguous gene syndromes. The ability to manipulate the mouse genome allows the systematic exploration of regions that are affected by alterations in gene dosage. To explore the effects of segmental haploidy in the mouse t complex on chromosome 17, radiation-induced deletion complexes centered at the Sod2 and D17Leh94 loci were generated in embryonic stem (ES) cells. A small interval was identified that, when hemizygous, caused specific embryonic lethal phenotypes (exencephaly and edema) in most fetuses. The penetrance of these phenotypes was background dependent. Additionally, evidence for parent-of-origin effects was observed. This genetic approach should be useful for identifying genes that are imprinted or whose dosage is critical for normal embryonic development.

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