Direct fluorescent glycan labeling with recombinant sialyltransferases
Abstract Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and detection of N- and O-glycans on glycoproteins. The method allows detection of specific glycans without the laborious gel blotting and chemiluminescence reactions used in Western blotting. The method can also be used with a variety of fluorescent dyes.
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1997 ◽
Vol 161
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pp. 299-311
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1974 ◽
Vol 32
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pp. 210-211
1988 ◽
Vol 46
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pp. 40-41
1995 ◽
Vol 53
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pp. 54-55
1995 ◽
Vol 53
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pp. 864-865
1993 ◽
Vol 51
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pp. 132-133
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1993 ◽
Vol 51
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pp. 172-173
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