Thin Layer Chromatography of Dinitrophenol Pesticides
Keyword(s):
Uv Light
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Abstract Four dinitrophenol pesticides, DNOC, DNBP, DNAP, and DNOCHP, can be separated on cellulose thin layer plates, either as the phenols or their methyl ethers. Methyl ethers are prepared by reaction with diazomethane. Partition chromatography between an immobile phase of mineral oil and a mobile aqueous phase gives complete resolution. The compounds are revealed by spraying first with stannous chloride, then with p-dimethylaminobenzaldehyde to yield yellow-orange spots which fluoresce under UV light. The lower limit of detection in the phenolic form varies with the compound, ranging from 0.05 μg for DNOC to 0.3 μg for DNOCHP. The lower limit for the ethers is the same for all compounds, 0.1 pg.
2016 ◽
Vol 8
(12)
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pp. 190
Keyword(s):
1996 ◽
Vol 79
(3)
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pp. 656-660
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1994 ◽
Vol 77
(4)
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pp. 939-941
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1993 ◽
Vol 76
(2)
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pp. 335-341
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