A Rapid Thin Layer Chromatographic Method for Simultaneous Screening of Albendazole and Ivermectin in Formulations Prescribed for Human or Veterinary Use

An easy and selective thin layer chromatographic method has been developed and experimentally validated for the simultaneous screening of most commonly used anthelmintic drugs i.e. albendazole and ivermectin. Separation of these compounds was attained on silica gel 60 F254 pre-coated thin layer chromatographic plate using an optimized mobile phase of diisopropyl ether:ethyl acetate:glacial acetic acid in the ratio of 7:3:0.1 (v/v), respectively at pH 3.5. The calculated Rf values for albendazole and ivermectin were 0.65 and 0.38, respectively and the LOD was found to be 25 μg/ml and 30 μg/ml for albendazole and ivermectin, respectively. The developed method is selective, sensitive, robust, cost effective, eco-friendly, rapid as well as easy to perform. The developed method was successfully applied for the analysis of albendazole and ivermectin in pharmaceutical preparations marketed as oral suspensions, powder, tablets and injectable of the single or combined dosage forms for human as well as veterinary use. It could also be applied for the simultaneous analysis of both the compounds in other samples.

Application of Overpressured Layer Chromatography Combined with Digital Autoradiography and Mass Spectrometry in the Study of Deramciclane Metabolism

Overpressured layer chromatography was combined with the highly sensitive and rapid digital autoradiography (DAR) and mass spectrometry to separate, detect, and identify 3H-and 14C-labeled deramciclane metabolites in different biological matrixes. Several minor and major metabolites were separated from plasma and urine samples. The radioactive metabolites localized by DAR were scraped from the thin-layer chromatographic plate and transferred to a mass spectrometer for structure identification. Several metabolites were isolated and characterized, including hydroxy-/V-des- methyl deramciclane, which is described in detail. The combination of techniques is efficient and has good sensitivity: about 2 μg metabolite from a biological matrix was isolated and identified this way.

Quantitation of Sulfamethazine in Pork Tissue by Thin-Layer Chromatography

Our earlier method to detect and quantitate sulfamethazine (SMZ) in milk at the 10 ppb level was modified to quantitate SMZ in pork tissue. Sulfabromomethazine (SBZ) is added to the tissue as an internal standard. SMZ and SBZ are extracted from the tissue into water as the supernatant of a centrifuged, aqueous homogenate and are cleaned up and concentrated by a series of solid-phase extractions. The sulfonamide-containing eluate is then separated on a silica gel thin-layer chromatographic plate. SBZ and SMZ are derivatized with fluorescamine, and their fluorescence is quantitated with a scanning densitometer. The limit of detection was estimated at 0.25 ppb (signal-to-noise ratio, 3:1). The average accuracy over the analysis range (0.54-21.8 ppb [μg/kg]) was 95.6% (standard deviation = 29.4%, n = 54).

Laser-Based Photoacoustic Densitometer for Two-Dimensional Scanning of Thin-Layer Chromatographic Plates

Laser-based photoacoustic densitometry was applied to two-dimensional analysis in thin-layer chromatography. An acousto-optic device was used to provide both intensity modulation and a rastering scan. The spatial resolution is 25 × 60 spots for an area of 25 × 50 mm on the thin-layer chromatographic plate. Three detection modes, normal modulation, resonant modulation, and unmodulated rapid scan modes were compared. Detection limits in the most sensitive mode and in the fastest mode are 350 pg with scan time of 153 s and 6.9 ng with scan time of 1.5 s, respectively.