Differentiation of Randhuni (Apium graveolens) and Ajowan (Carum copticum) by Thin Layer Chromatographic Separation of Their Essential Oils

Abstract The volatile oils of randhuni and ajowan seeds are extracted and spotted on silica gel G thin layer plates. The plates are developed in either benzene-petroleum ether (1+1) or chloroform-pyridine-benzene (65+5+30). Spraying with anisaldehyde-sulfuric acid reagent shows a single red spot for ajowan and 4 spots (violet, greenish yellow, violet, and blue) for randhuni. This procedure can also differentiate admixtures of the 2 and seeds of other Umbelliferae.

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Thin Layer Chromatographic Detection of Carum bulbocastanum Koch in Carum carvi Linn

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/60.1.235 ◽

1977 ◽

Vol 60 (1) ◽

pp. 235-236

Author(s):

Asit R Sen ◽

Pranab K Sardar ◽

Pratima Sengupta

Keyword(s):

Thin Layer ◽

Essential Oils ◽

Petroleum Ether ◽

Silica Gel ◽

Chromatographic Method ◽

Carum Carvi ◽

Chromatographic Detection

Abstract A rapid thin layer chromatographic method has been developed to detect Carum bulbocastanum Koch in Carum carvi Linn. The volatile essential oils are developed on silica gel G plates, using benzene-petroleum ether and visualized by 2,4-dinitrophenylhydrazine spray. This procedure can also differentiate mixtures of the 2 seeds.

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A Validated Quantitative Thin-Layer Chromatographic Method for Estimation of Diosgenin in Various Plant Samples, Extract, and Market Formulation

Journal of AOAC International ◽

10.1093/jaoac/90.2.358 ◽

2007 ◽

Vol 90 (2) ◽

pp. 358-363 ◽

Cited By ~ 14

Author(s):

Priti D Trivedi ◽

Kilambi Pundarikakshudu ◽

Shivprakash Rathnam ◽

Karishma S Shah

Keyword(s):

Sulfuric Acid ◽

Thin Layer ◽

Chromatographic Method ◽

Detection Sensitivity ◽

Fenugreek Seed ◽

Plant Samples ◽

Relative Standard ◽

Leaf Powder ◽

Acid Reagent

Abstract A sensitive and reproducible thin-layer chromatographic method has been developed for quantitation of diosgenin, a spiroketal sapogenin. The spots were visualized by spraying with modified anisaldehyde-sulfuric acid reagent. The concentration of anisaldehyde was reduced to 0.1% instead of 1%, and the concentration of sulfuric acid was kept at a minimum of 2%. This successfully reduced charring and background interference. The method was validated according to International Conference on Harmonization guidelines. The method was used for determination of diosgenin from dried samples of fenugreek seeds, leaves, stem, seed extracts, and a polyherbal antidiabetic formulation containing fenugreek powder as one of the ingredients. Increased detection sensitivity was observed with linearity from 98 to 588 ng/spot and a correlation coefficient (r2) of 0.988. The relative standard deviation value for linearity of the method was found to be 0.18%. The method was successfully applied to various plant samples of fenugreek (Methi) with a recovery of 98.11 ± 1.4%. Dried plant samples and a market formulation were analyzed and found to contain diosgenin in the range of 0.5290.658% (w/w) in fenugreek seed powders, 0.087% (w/w) in fenugreek leaf powder, 0.015 and 1.27% (w/w) in fenugreek stem powder and extract, respectively, and 0.586% (w/w) in a formulation containing fenugreek seed powder. No matrix interference was observed.

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Determination of 1,8-Cineole in Eucalyptus Essential Oils by Thin-Layer Chromatography and Densitometry

Journal of AOAC International ◽

10.1093/jaoac/78.1.115 ◽

1995 ◽

Vol 78 (1) ◽

pp. 115-117 ◽

Cited By ~ 1

Author(s):

Carmen Rossini ◽

Enrique Pandolfi ◽

Eduardo Dellacassa ◽

Patrick Moyna

Keyword(s):

Liquid Chromatography ◽

Thin Layer ◽

Essential Oils ◽

Thin Layer Chromatography ◽

Mobile Phase ◽

Light Petroleum ◽

Gas Liquid Chromatography ◽

Acid Reagent ◽

Layer Chromatography

Abstract A thin-layer chromatography (TLC) method with scanning is described for evaluation of the 1,8-cineole content of Eucalyptus essential oils. Samples were developed on silica gel G 60 with light petroleum–chloroform (70 + 30) as mobile phase. Color was developed with 4-dimethylaminobenzal-dehyde–sulfuric acid reagent. The absorbance of colored spots was measured at 460 nm. The response was linear between 15 to 75 μg of 1,8-cineole, with a correlation coefficient of 0.9997. The results of each assay were compared with those from gas–liquid chromatography (GLC). The TLC to GLC ratio was constant (0.92).

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Carum copticum L. essential oils as natural antimicrobial and antioxidant

Bangladesh Journal of Botany ◽

10.3329/bjb.v44i4.38564 ◽

2018 ◽

Vol 44 (4) ◽

pp. 515-520

Author(s):

M Kazemi

Keyword(s):

Essential Oils ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Carum Copticum ◽

Microbial Strains ◽

Pharmaceutical Industries ◽

Dpph Radical Scavenging ◽

Antioxidant Effects

Carum copticum L. comprises several relevant species for food, cosmetic, perfumery and pharmaceutical industries. GC/MS analysis of the enential oil of C. copticum revealed γ- terpinene as a major component of C. copticum, with its contribution of 33.85%. Essential oils (EOs) exhibited a significant antimicrobial activity against all tested microbial strains. In addition, the C. copticum oil demonstrated the highest DPPH radical scavenging activity. These results clearly show the antimicrobial and antioxidant effects of the plant essential oil.

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