Determination of Nutritional and Bioactive Properties of Peptides in Enzymatic Pea, Chickpea, and Mung Bean Protein Hydrolysates

Abstract Within the primary structure of many pea and mung bean proteins are peptide sequences that can potentially be used in the formulation of therapeutic products for the treatment and prevention of human diseases. However, these peptide sequences need protease treatments before they can be released free of the parent proteins. Unlike chemical hydrolysis, enzymatic treatment enables more efficient tailoring of peptide products without formation of toxic by-products or destruction of amino acids. This review provides information on current methods that have been used to convert inactive pea and mung bean proteins into bioactive peptides. It focuses on 3 main bioactive properties, such as inhibitions of (1) angiotensin converting enzyme (ACE) activity; (2) calmodulin (CaM)-dependent enzymes; and (3) copper-chelating activity. ACE is an established marker for hypertension, high levels of some CaM-dependent enzymes are risk factors for various human diseases including cancer and Alzheimer's disease, and high vascular copper concentrations may potentiate atherosclerosis. Also reviewed are the production and evaluation of activity of hypoallergenic peptides that may offer protection against anaphylactic reactions. The 3 main proteins discussed are chickpea, mung bean, and field pea.

Download Full-text

Characterization of Protein Hydrolysates from Fish Discards and By-Products from the North-West Spain Fishing Fleet as Potential Sources of Bioactive Peptides

Marine Drugs ◽

10.3390/md19060338 ◽

2021 ◽

Vol 19 (6) ◽

pp. 338

Author(s):

Andreia Henriques ◽

José A. Vázquez ◽

Jesus Valcarcel ◽

Rogério Mendes ◽

Narcisa M. Bandarra ◽

...

Keyword(s):

Inhibitory Activity ◽

Bioactive Peptides ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Protein Hydrolysates ◽

Scavenging Activity ◽

North West ◽

Chelating Activity ◽

The North ◽

By Products

Fish discards and by-products can be transformed into high value-added products such as fish protein hydrolysates (FPH) containing bioactive peptides. Protein hydrolysates were prepared from different parts (whole fish, skin and head) of several discarded species of the North-West Spain fishing fleet using Alcalase. All hydrolysates had moisture and ash contents lower than 10% and 15%, respectively. The fat content of FPH varied between 1.5% and 9.4% and had high protein content (69.8–76.6%). The amino acids profiles of FPH are quite similar and the most abundant amino acids were glutamic and aspartic acids. All FPH exhibited antioxidant activity and those obtained from Atlantic horse mackerel heads presented the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and Cu2+ chelating activity. On the other hand, hydrolysates from gurnard heads showed the highest ABTS radical scavenging activity and Fe2+ chelating activity. In what concerns the α-amylase inhibitory activity, the IC50 values recorded for FPH ranged between 5.70 and 84.37 mg/mL for blue whiting heads and whole Atlantic horse mackerel, respectively. α-Glucosidase inhibitory activity of FPH was relatively low but all FPH had high Angiotensin Converting Enzyme (ACE) inhibitory activity. Considering the biological activities, these FPH are potential natural additives for functional foods or nutraceuticals.

Download Full-text

Evaluation of Functional, Nutritional and Bioactive Properties of Gluten Free Muffins Utilizing Industrial By-Products of Baby Corn

Edelweiss Journal of Food Science and Technology ◽

10.33805/2765-8821.108 ◽

2021 ◽

pp. 1-6

Author(s):

Kaur Kamaljit ◽

Sharma Anchit

Keyword(s):

Finger Millet ◽

Antioxidant Properties ◽

Pasting Properties ◽

Total Phenol Content ◽

Gluten Free ◽

Corn Flour ◽

Chelating Activity ◽

Metal Chelating ◽

Bioactive Properties ◽

By Products

This study was planned to utilize by-products of baby corn, which was powdered upon drying. Different formulations containing baby corn: defatted soya: plantain: finger millet flours at 10:65:20:5, 15:55:20:10, 20:45:20:15 and 25:35:20:20 were prepared and analyzed for functional, nutritional and bioactive properties of the blended flours and muffins. The outcomes indicated that incorporation of baby corn flour had enhanced functional and pasting properties of the blended flours. The addition of baby corn flour increased the antioxidant properties and metal chelating activity of blended flours. The incorporation of baby corn flour had contributed better retention of antioxidant potential during baking as muffins with 25% baby corn showed more increase in antioxidant properties than 10% baby corn muffins. Hardness and specific volume of muffins increased, while total phenol content decreased significantly with increment in the level of baby corn flour. On the basis of sensory analysis muffins prepared with 20% level of baby corn flour was selected best. This study concluded that by-products of baby corn can be utilized for development of gluten free muffins with better nutrition and bioactive properties.

Download Full-text

Modern determinants of endometrium hyperplastic processes pathogenesis

HEALTH OF WOMAN ◽

10.15574/hw.2016.111.137 ◽

2016 ◽

pp. 137-142

Author(s):

V.O. Benyuk ◽

◽

V.M. Goncharenko ◽

T.R. Nykoniuk ◽

◽

...

Keyword(s):

Conservative Therapy ◽

Solid Phase ◽

Research Direction ◽

Immunohistochemical Method ◽

Results Of Treatment ◽

Treatment And Prevention ◽

Receptor Phenotype ◽

The Relationship ◽

Endometrial Proliferation

The objective: to еxplore the relationship between the activity of endometrial proliferation and the state of the local immune response in the uterus in the conditions berprestasi process. Patients and methods. Examined 228 women of reproductive and perimenopausal age with endometrial pathology using ultrasound and then performing hysteroresectoscopy. Determination of the concentrations of the cytokines IL-1, IL-2, IL-6 and TNF was performed by solid phase ELISA. Results. Found a trend that confirms the loss of sensitivity to hormones at the stage of malignancy of the endometrium and can be used as diagnostic determinants in determining the nature of intrauterine pathology and criterion of the effectiveness of conservative therapy. Conclusion. Improving etiopatogenetice approach to the therapy of hyperplastic proce.sses of endometrium with determination of receptor phenotype of the endometrium is a research direction in modern gynecology, which will help to improve the results of treatment and prevention of intrauterine pathology. Key words: endometrial hyperplasia,the receptors for progesterone and estrogen, immunohistochemical method.

Download Full-text

Cereals, pulses and by-products. Determination of ash yield by incineration

10.3403/30145278 ◽

2007 ◽

Keyword(s):

By Products

Download Full-text

Methods of test for starch and its derivatives and by-products. Determination of nitrogen conteny by the Kjeldahl method ( spectrophotometric method)

10.3403/30309457 ◽

1979 ◽

Keyword(s):

Spectrophotometric Method ◽

Kjeldahl Method ◽

By Products

Download Full-text

A Simple Spectrofluorometric Method for the Determination of Total Auxins in Mung Bean Sprouts

Journal of Analytical Chemistry ◽

10.1134/s1061934820110155 ◽

2020 ◽

Vol 75 (11) ◽

pp. 1404-1407

Author(s):

Aziguli Yigaimu ◽

Jiahua Chang ◽

Amina Hoji ◽

Turghun Muhammad ◽

Burabiye Yakup ◽

...

Keyword(s):

Mung Bean ◽

Mung Bean Sprouts ◽

Spectrofluorometric Method ◽

Bean Sprouts

Download Full-text

Unveiling Putative Functions of Mucus Proteins and Their Tryptic Peptides in Seven Gastropod Species Using Comparative Proteomics and Machine Learning-Based Bioinformatics Predictions

Molecules ◽

10.3390/molecules26113475 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3475

Author(s):

Viroj Tachapuripunya ◽

Sittiruk Roytrakul ◽

Pramote Chumnanpuen ◽

Teerasak E-kobon

Keyword(s):

Bioactive Peptides ◽

Nearest Neighbor ◽

Peptide Identification ◽

Comparative Proteomics ◽

Mass Spectrometric ◽

Functional Categories ◽

K Nearest Neighbor ◽

Gastropod Species ◽

Bioactive Properties ◽

Sds Page

Gastropods are among the most diverse animals. Gastropod mucus contains several glycoproteins and peptides that vary by species and habitat. Some bioactive peptides from gastropod mucus were identified only in a few species. Therefore, using biochemical, mass spectrometric, and bioinformatics approaches, this study aimed to comprehensively identify putative bioactive peptides from the mucus proteomes of seven commonly found or commercially valuable gastropods. The mucus was collected in triplicate samples, and the proteins were separated by 1D-SDS-PAGE before tryptic digestion and peptide identification by nano LC-MS/MS. The mucus peptides were subsequently compared with R scripts. A total of 2818 different peptides constituting 1634 proteins from the mucus samples were identified, and 1218 of these peptides (43%) were core peptides found in the mucus of all examined species. Clustering and correspondence analyses of 1600 variable peptides showed unique mucous peptide patterns for each species. The high-throughput k-nearest neighbor and random forest-based prediction programs were developed with more than 95% averaged accuracy and could identify 11 functional categories of putative bioactive peptides and 268 peptides (9.5%) with at least five to seven bioactive properties. Antihypertensive, drug-delivering, and antiparasitic peptides were predominant. These peptides provide an understanding of gastropod mucus, and the putative bioactive peptides are expected to be experimentally validated for further medical, pharmaceutical, and cosmetic applications.

Download Full-text

Proteins and Bioactive Peptides in High Protein Content Foods

Foods ◽

10.3390/foods10061186 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1186

Author(s):

Fidel Toldrá ◽

Leticia Mora

Keyword(s):

Enzymatic Hydrolysis ◽

Protein Content ◽

Human Body ◽

Bioactive Peptides ◽

High Protein ◽

Microbial Fermentation ◽

Gastrointestinal Digestion ◽

High Protein Content ◽

By Products

Foods and their industry by-products constitute very good sources of bioactive peptides, which can be naturally generated during processing but are also extensively produced through enzymatic hydrolysis, microbial fermentation, and even during gastrointestinal digestion in the human body [...]

Download Full-text