scholarly journals Enzyme-Linked Immunosorbent Assay Kit for Beta-Lactoglobulin Determination: Interlaboratory Study

2009 ◽  
Vol 92 (5) ◽  
pp. 1519-1525 ◽  
Author(s):  
František Štumr ◽  
Dana Gabrovská ◽  
Jana Rysová ◽  
Petr Hanák ◽  
Jan Plicka ◽  
...  
Keyword(s):  
Wheat Flour ◽  
Statistical Tests ◽  
Milk Proteins ◽  
Food Samples ◽  
Chicken Meat ◽  
Interlaboratory Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Study Results ◽  
Two Samples ◽  
Beta Lactoglobulin

Abstract An interlaboratory study was performed in six laboratories to prove the validation of the ELISA method developed for quantitative determination of beta-lactoglobulin (BLG) in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. In-house validation of the kit did not produce false-positive results or cross-reactivity in a broad range of food matrixes containing no milk proteins. All participants obtained the BLG kit with a standard operational procedure, the list of the samples, samples, and a protocol for recording test results. The study included 14 food samples (extruded breakfast cereals, bread, two soy desserts, butter, chicken ham, chicken meat, wheat flour, long grain rice, jelly, two whey drinks, crackers, and bitter chocolate) and six spiked samples (two rice, two wheat flour, and two chicken meat). Nine samples of food matrixes containing no milk proteins showed BLG content lower than the first standard (0.15 mg/kg). Two samples of food matrixes with no milk proteins revealed BLG content higher than standard 3 (1.5 mg/100 g) and standard 4 (5.0 mg/100 g). Three food samples containing milk were tested as positive, and all spiked samples were evaluated as positive. The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as LOQ (0.22 mg BLG/kg) and LOD (0.07 mg BLG/kg), for the kit were calculated.

scholarly journals ELISA Kit for Mustard Protein Determination: Interlaboratory Study

2011 ◽  
Vol 94 (2) ◽  
pp. 605-610 ◽  
Author(s):  
Petr Cuhra ◽  
Dana Gabrovská ◽  
Jana Rysová ◽  
Petr Hanák ◽  
Frantiek Štumr ◽  
...  
Keyword(s):  
Protein Content ◽  
Statistical Tests ◽  
Food Samples ◽  
Cross Reactivity ◽  
Interlaboratory Study ◽  
Protein Determination ◽  
Elisa Kit ◽  
Study Results ◽  
Rabbit Polyclonal Antibody ◽  
Mustard Protein

Abstract An interlaboratory study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative determination of mustard protein in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false-positive results or cross-reactivity with in-house validation for a broad range of food matrixes with no detectable mustard protein. All participants obtained the Mustard ELISA kit with standard operational procedures, a list of samples, samples, and a protocol for recording test results. The study included 15 food samples and two spiked samples. Seven food matrix samples of zero mustard content and four samples with mustard declared as an ingredient showed mustard protein content lower than that of the first standard (0.42 mg/kg). Four samples with mustard declared as an ingredient revealed mustard protein content above 12.5 mg/kg (the highest standard). The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as an LOQ (1.8 mg mustard proteins/kg) and LOD (0.5 mg mustard proteins/kg), for the kit were calculated.

scholarly journals ELISA Kit for Determination of Egg White Proteins: Interlaboratory Study

2010 ◽  
Vol 93 (6) ◽  
pp. 1923-1929
Author(s):  
Kvìta Tomková ◽  
Petr Cuhra ◽  
Jana Rysová ◽  
Petr Hanák ◽  
Dana Gabrovská ◽  
...  
Keyword(s):  
Statistical Tests ◽  
Egg White ◽  
Food Samples ◽  
Cross Reactivity ◽  
Interlaboratory Study ◽  
Food Matrix ◽  
Elisa Kit ◽  
Egg White Proteins ◽  
Study Results

Abstract An interlaboratory study was conducted in 11 laboratories to validate an ELISA method developed for the quantitative determination of egg white proteins (EWPs) in foods. The ELISA kit used for this study is based on sheep polyclonal antibody. It does not produce any false-positive results or cross-reactivity in a broad food matrix range with zero EWP content. All participants obtained the Egg ELISA Kit-native with standard operational procedure and the list of samples, as well as the samples and a protocol for recording test results. The study included 10 food samples. Four samples of food matrix with zero EWP content showed EWP content lower than the first standard (EWP content 0.5 mg/kg). One sample of food matrix with zero EWP content revealed EWP content higher than standard 3 (1.5 mg EWP/kg). Five food samples containing EWP as an ingredient tested positive and one negative. The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as LOQ (1.4 mg EWP/kg) and LOD (0.43 mg EWP/kg), were calculated for the kit.

Validated Sandwich Enzyme-Linked Immunosorbent Assay for Casein and Its Application to Retail and Milk-Allergic Complaint Foods

2004 ◽  
Vol 67 (9) ◽  
pp. 1933-1938 ◽  
Author(s):  
SUSAN L. HEFLE ◽  
DEBRA M. LAMBRECHT
Keyword(s):  
Immunosorbent Assay ◽  
Milk Proteins ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Ingredients ◽  
Milk Contamination ◽  
Quality Control Tool ◽  
Sandwich Type ◽  
Life Threatening ◽  
Phosphate Buffered Saline

Cows' milk is a commonly allergenic food. Cross-contamination of milk proteins into nondairy, kosher-pareve foods prepared on shared processing equipment can cause severe, life-threatening reactions in milk-allergic individuals. A sandwich-type enzyme-linked immunosorbent assay (ELISA; 96-well plate format) was developed for the detection of undeclared casein in foods. Rabbit anti-casein antibodies were used as the capture reagent. Food samples and standards were ground, extracted in 0.01 M phosphate-buffered saline, clarified by centrifugation, and added to the wells. Goat anti-casein antibodies were employed as the detector antibody, and the amount of antibody bound was determined with a commercial rabbit anti-goat immunoglobulin conjugated to alkaline phosphatase, with subsequent substrate reaction. Antibodies developed were specific to casein, with no cross-reaction observed with 30 foods and food ingredients. Non–milk-containing products such as fruit juices, fruit juice bars, sorbets, and dark and pareve-labeled chocolate were purchased from June 2002 through June 2003. In addition, samples allegedly causing eight milk-allergic consumer complaints were analyzed. The ELISA had a detection limit of less than 0.5 ppm of casein. The casein content in the analyzed foods ranged from less than 0.5 ppm to more than 40,000 ppm casein; undeclared casein residues were found in all of the samples implicated in allergic reactions. The levels of milk contamination in some of the other surveyed products could also be hazardous for milk-allergic consumers. This ELISA method provides a useful quality control tool for the food industry and could also be used as a validation of kosher-pareve status.

scholarly journals Cow’s milk proteins immunoreactivity and allergenicity in processed food

2012 ◽  
Vol 30 (No. 3) ◽  
pp. 211-219 ◽  
Author(s):  
B. Wróblewska ◽  
A. Kaliszewska
Keyword(s):  
Milk Proteins ◽  
Cottage Cheese ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Food ◽  
Uht Milk ◽  
Sds Page ◽  
Specific Reactions ◽  
Beta Lactoglobulin ◽  
Hard Cheese ◽  
Page Electrophoresis

The immunoreactivity and allergenicity of proteins present in processed food (UHT milk, yoghurt, hard cheese, cottage cheese, biscuit, and sausage intended for children) were determined in this study. Proteins were characterised by SDS-PAGE electrophoresis. The changes in immunoreactivity were compared by enzyme-linked immunosorbent assay (ELISA) using polyclonal rabbit antibodies specific to α-lactalbumin (α-la), β-lactoglobulin (β-lg), α-, β-, and κ-casein. The allergenicity was determined with human pooled sera from CMA allergic patients by ELISA and immunoblot. The results have shown that the allergenicity of the food products is mainly correlated with bovine serum albumin (BSA), lactofferin (LF), and α-casein or the products of non-specific reactions between carbohydrate and proteins (e.g. lactosylation).  

scholarly journals Gluten Determination by Gliadin Enzyme-Linked Immunosorbent Assay Kit: Interlaboratory Study

2006 ◽  
Vol 89 (1) ◽  
pp. 154-160 ◽  
Author(s):  
Dana Gabrovská ◽  
Jana Rysová ◽  
Vanda Filová ◽  
Jan Plicka ◽  
Petr Cuhra ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Statistical Tests ◽  
Cross Reactivity ◽  
Interlaboratory Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Results ◽  
Analysis And Evaluation ◽  
Elisa Kit ◽  
Limit Of Quantitation ◽  
And Performance

Abstract An interlaboratory study with 10 participants was performed to obtain validation and performance data for an enzyme-linked immunosorbent assay (ELISA) kit developed for quantitative gluten determination in foods. The ELISA kit used for this study is based on 2 monoclonal and 1 polyclonal antibody developed by Immunotech, a Beckman Coulter Co. This kit did not show any false positive results or cross-reactivity with oat, rice, maize, and buckwheat. The gliadin standard from the Working Group on Prolamin Analysis and Toxicity was included in the kit as reference material for calibration. All participants obtained a gliadin ELISA kit with Standard Operational Procedure and a form for recording test results. The study included 13 samples labeled as gluten-free and 2 samples spiked by wheat flour. Seven samples had gliadin content below the limit of quantitation (LOQ) of the method, and 1 sample exceeded the highest calibration level. Gliadin content in the range from 10 to 157 mg/kg (1st day) and from 11 to 183 mg/kg (2nd day) was found in 7 samples (including 2 spiked samples). Results of these samples were used for further statistical analysis and evaluation. The Cochran, Dixon, and Mandel statistical tests were applied for detection of outliers. The LOQ of the kit was estimated.

Pilot study for cystic fibrosis neonatal screening: the Cuban experience

2020 ◽  
Vol 58 (11) ◽  
pp. 1857-1864
Author(s):  
Elisa M. Castells ◽  
Aramis Sánchez ◽  
Amarilys Frómeta ◽  
Yanin Mokdse ◽  
Nelson Ozunas ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Screening Program ◽  
Dried Blood Spots ◽  
Enzyme Linked Immunosorbent Assay ◽  
Perinatal Factors ◽  
Screening Process ◽  
Unequal Variance ◽  
National Network ◽  
Two Samples ◽  
The Mean

AbstractBackgroundIn Cuba, no screening program for cystic fibrosis (CF) has been implemented yet. The ultramicro enzyme-linked immunosorbent assay (UMELISA)® TIR NEONATAL has been developed for the measurement of immunoreactive trypsin (IRT) in dried blood spots on filter paper. The analytical performance of the kit was evaluated in the national network of laboratories.MethodsNewborn dried blood samples (DBS) were evaluated in 16 laboratories. An IRT/IRT/DNA protocol was followed using a cut-off value of 50 ng/mL. The mean, median and percentiles of the distribution were calculated and a two-sample t-test with unequal variance was used for statistical analysis. Influence of perinatal factors on IRT levels was analyzed.ResultsFrom January to June 2018, 6470 newborns were studied, obtaining a mean IRT value of 12.09 ng/mL (ranging 0–358 ng/mL) and a median of 8.99 ng/mL. Fifty-two samples (0.78%) were above the cut-off level and 16 samples (0.24%) were elevated in the re-screening process. One of them was confirmed positive by molecular biology (phe508del/c.3120 + 1G > A), constituting the first newborn screened and diagnosed early in Cuba. Second DBS samples were collected on average at 14 days and processed in the laboratory at 16 days of birth. Significant differences were observed (p < 0.05) when evaluating the influence of gender, birth weight (BW) and gestational age (GA) on the IRT values. Lower IRT concentrations were found in samples processed after 10 days of collection.ConclusionsThe performance of UMELISA® TIR NEONATAL in the laboratories has been satisfactory; hence CF newborn screening (NBS) was extended throughout the country from January 2019.

Ultrasensitive determination of chloramphenicol in pork and chicken meat samples using a portable electrochemical sensor: effects of 2D nanomaterials on the sensing performance and stability

2021 ◽  
Author(s):  
Ngo Xuan Dinh ◽  
Tuyet Nhung Pham ◽  
Tran Quang Huy ◽  
Do Quang Trung ◽  
Pham Anh Tuan ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Food Samples ◽  
Chicken Meat ◽  
Electrochemical Sensing ◽  
Portable Sensors ◽  
2D Nanomaterials ◽  
Meat Samples ◽  
Sensing Performance

This work contributes to a deeper understanding of the effects of functional 2D nanomaterials on the electrochemical sensing performance of SPE-based portable sensors for the rapid, accurate, and on-site determination of CAP in food samples.

Case of oral mite anaphylaxis: Contamination of wheat flour by mites determined by enzyme‐linked immunosorbent assay

2021 ◽  
Author(s):  
Yukari Matsumoto ◽  
Shinya Imamura ◽  
Ayako Fukumoto ◽  
Kasumi Lee ◽  
Nao Nishitani ◽  
...  
Keyword(s):  
Wheat Flour ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

The Effectiveness of Durante Hemodialysis Packed Red Cells (PRC) Transfusion on Hemoglobin Levels

2021 ◽  
Vol 8 (1) ◽  
pp. 013-018
Author(s):  
Pria Wahyu Romadhon Girianto ◽  
Mega Wahyu Mulyasari
Keyword(s):  
Renal Disease ◽  
Chronic Renal Disease ◽  
Statistical Tests ◽  
Health Condition ◽  
P Value ◽  
Test Design ◽  
Significance Level ◽  
Study Results ◽  
Post Test ◽  
The One

Renal Disease was a chronic disease that the most attacking people in Indonesia. Damage to this vital organ in the human body greatly affected a person's health condition, one of which was anemia. This study aimed to determine the effectiveness of Durante hemodialysis PRC transfusions on hemoglobin levels. The method used was Pre-experimental design, with the One group pre-post test design approach. With a sample of 49 patients who underwent regular hemodialysis at RSUD dr. Iskak Tulungagung. Data were obtained by direct observation. Processed by computerized methods with a statistical t-test, the significance level (α) was 0.05. The study results showed that the hemoglobin levels of the pre-Durante hemodialysis PRC transfusion patients were 4-5 mg/dl (53.06%), and the hemoglobin levels of the post-Durante hemodialysis PRC transfusion patients were 6.1-7 mg/dl (34.69%). The results of statistical tests showed that there was an increase in hemoglobin levels in patients who received Durante hemodialysis PRC transfusion by 1.22 mg/dl because p-value = 0.000 < 0.05 (α) means that there was an effect. It could be concluded that the delivery of Durante hemodialysis PRC transfusion could help increase hemoglobin levels. This finding was very helpful for chronic Renal Disease patients undergoing hemodialysis, who have been using erythropoietin preparations because PRC transfusions were cheaper and more effective when compared to using erythropoietin preparations

scholarly journals Understanding New Middle Eastern Leadership: An Operational Code Approach

2017 ◽  
Vol 71 (1) ◽  
pp. 19-31 ◽  
Author(s):  
Özgür Özdamar ◽  
Sercan Canbolat
Keyword(s):  
Foreign Policy ◽  
Statistical Tests ◽  
Belief Systems ◽  
Middle Eastern ◽  
Foreign Affairs ◽  
Post Cold War ◽  
Operational Code ◽  
Arab Uprisings ◽  
Study Results ◽  
The Muslim Brotherhood

Political Islam and Islamist organizations have broadly gained strength across the Middle East and North Africa (MENA) in the post-Cold War era. Following the Arab uprisings, the Muslim Brotherhood (MB), generally viewed as the world’s largest and most influential Islamist organization, has shaped the wider landscape of MENA politics. This study examines MB leadership by comparing M. Morsi of Egypt, R. Ghannouchi of Tunisia, and K. Meshaal of Gaza as examples of Islamist leaders to explain their political belief systems and predict their foreign-policy behavior. We use the operational code approach, a content-analysis software and statistical tests to conduct the study. Results show that the three leaders’ foreign policy beliefs are analogous to the averages of world leaders. Results also partially support the hypothesis that their foreign-policy propensities are similar to each other. We conclude that despite the conventional portrayal of MB leadership, these leaders use negotiation and cooperation to settle their differences in foreign affairs, and the best way to approach them is to engage in a Rousseauvian assurance game that emphasizes international social cooperation. Results also suggest important implications in terms of mainstream international relations theories.

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