scholarly journals PSX-29 Late-Breaking Abstract: The effectiveness of in vivo determination of meat quality using an ultrasonic scanning device in Kazakhstan

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 354-355
Author(s):  
Anuarbek Bissembayev ◽  
Nurzhan Abuyev ◽  
Anuarbek Seitmuratov ◽  
Altay Nazarbekov ◽  
Saule Zhali
Keyword(s):  
Meat Quality ◽  
Live Weight ◽  
Beef Quality ◽  
Eye Muscle ◽  
Ultrasonic Scanning ◽  
Scanning Device ◽  
Slaughter Cattle ◽  
Ribeye Area

Abstract Improving beef quality is important task for livestock in Kazakhstan. Almost all indicators characterizing the quality of carcasses and beef are evaluated after slaughter. Their use in breeding is limited (Legoshin G.P. 2010). For lifetime assessment of carcasses in pedigree and slaughter cattle, Aloka 500B, EXAGO, EVO ultrasonographs are used, using which there is a high coincidence of the lifetime forecast of muscular development over the eye muscle area with the indicator in carcasses after slaughter of animals (Bisembaev A.T. 2019). A high correlation of prognosis of ribeye area with live weight of animals was noted (Lisitsyn A.B. 2010). The aim of the project is to study the effectiveness of in vivo determination of meat quality using an ultrasonic scanning device. Tasks: to determine the ribeye area, the fat of the cattle using an EXAGO ultrasonograph; compare the ribeye area, the fat of the slaughter cattle, determined using an EXAGO ultrasonograph and measured on the carcass after slaughter. The studies were carried out on pedigree bull-calves of the Kazakh white head (85 animals), Auliekol (101 animals) breeds aged 14–15 months and on the feeding stock (6 animals) with a live weight of more than 943 lb. The ribeye area, the fat were obtained: Kazakh white head had 23.2 sq.in and 0.10 in, Auliekol – 20.4 sq.in and 0.09 in. The animals studied after slaughter yielded full-bodied carcasses, while the slaughter yield averaged 56.3%. The correlation between live weight level and ribeye was r=0.97. The coincidence of ribeye, measured by an ultrasonograph with a post-mortem measurement averaging 93.8%. The introduction of ultrasound methods for determining beef productivity in beef industry will allow livestock husbandry to become cost-effective and improve the beef quality. The results of ultrasound images for the carcass traits will be applied in selection and breeding work.

Effects of growth pathway on beef quality in 18-month-old Angus and South Devon ✕ Angus pasture-fed steers

2001 ◽  
Vol 72 (2) ◽  
pp. 297-308 ◽  
Author(s):  
P.D. Muir ◽  
N.B. Smith ◽  
P.M. Dobbie ◽  
D.R. Smith ◽  
M.D. Bown
Keyword(s):  
Meat Quality ◽  
Compensatory Growth ◽  
Live Weight ◽  
Considerable Effect ◽  
Growth Restriction ◽  
Carcass Weight ◽  
Winter Period ◽  
Meat Tenderness ◽  
Beef Quality ◽  
Significant Difference

AbstractThere is evidence that, in cattle, rapid compensatory growth after a period of growth restriction may increase the variability of beef quality and that variability is greater in breeds which reach larger body size at maturity. This experiment investigated the effect of compensatory growth following a period of winter growth restriction on carcass quality in steers of small and large mature size grazed on pasture. Angus (no. = 120) and South Devon ✕ Angus (SD✕ A; no. = 110) steers were allocated to non-restricted or restricted growth treatment groups and for 126 days over winter their grazing was managed to achieve mean live-weight gains of approximately 0·7 kg/day or to maintain live weight for each group respectively. Steers were slaughtered at the start (no. = 10 per breed) and end (no. = 20 per nutrition group (10 from each breed)) of the winter period for carcass evaluation. Thereafter, remaining steers were grazed together at a high pasture allowance to maximize growth and were slaughtered for carcass evaluation as they reached target live weights (Angus, 590 kg; SD✕ A, 620 kg). SD✕ A steers grew faster (P< 0·05) during both the winter and finishing periods and reached target slaughter live weights approximately 20 days earlier than Angus steers. SD✕ A steers also had leaner carcasses than Angus steers at each slaughter although there was no significant difference in meat quality between breeds. However, there was a significant effect of restriction treatment on carcass weight and meat quality. The non-restricted steers grew faster, had heavier carcasses and more tender steaks than restricted steers, although there was no significant difference in carcass fatness (adjusted for hot carcass weight). Muscle calpain activities were positively correlated with live-weight gain during the finishing period suggesting increased potential for post-mortem myofibrillar proteolysis and therefore increased meat tenderness. However, in the present experiment this was confounded by an increase in shear force with age-at-slaughter, especially in the non-restricted steers. Nevertheless, steers which were heaviest at the start of the experiment reached slaughter live weight earliest, were leaner than average and had higher calpain system activities at slaughter regardless of breed or restriction treatment. Furthermore, in the non-restricted group, high initial live weight and early slaughter was associated with lower pH and more tender meat. In conclusion, although restriction affected meat quality attributes, it appears that live weight prior to food restriction also had a considerable effect on carcass characteristics at slaughter. Therefore, the characteristics of the early growth phase prior to the finishing period may have important consequences for meat quality.

scholarly journals Quantitative and qualitative carcass characteristics of feedlot ewes subjected to increasing levels of concentrate in the diet

2014 ◽  
Vol 66 (5) ◽  
pp. 1601-1610 ◽  
Author(s):  
R.A.S. Cacere ◽  
M.G. Morais ◽  
F.V. Alves ◽  
G.L.D. Feijó ◽  
C.C.B.F. Ítavo ◽  
...  
Keyword(s):  
Meat Quality ◽  
Ultrasound Examination ◽  
Carcass Characteristics ◽  
Live Weight ◽  
Regression Analyses ◽  
Adult Weight ◽  
Fat Thickness

The aim of this study was to evaluate the effect of increasing levels of concentrate on the quantitative and qualitative carcass characteristics of feedlot ewes. Twenty-four crossbred ewes were randomly distributed in four treatments: (1) diet with 20%; (2) 40%; (3) 60%; and (4) 80% of concentrate. Animals were slaughtered when those receiving the diet with 80% of concentrate reached adult weight, at around 50kg LW. The data were analyzed by means of variance and regression analyses and correlations. The levels of concentrate increased live weight at slaughter linearly. Height at the withers and rump presented medium to high correlation (0.86). The correlation of loin eye area measured on the carcass and by ultrasound examination was 0.81, whereas for back fat thickness it was 0.85. The increase in the use of concentrate in the diets promotes heavier carcasses with better yields and fat cover. Some measures obtained in vivo presented high correlation with those measured on the carcass. The use of ultrasound can be a way to predict the in vivo loin eye area. The use of concentrate improves the meat quality in variables' color and appearance.

Determination of changes on tooth-colored cervical restorations in vivo using a three-dimensional laser scanning device

2000 ◽  
Vol 108 (3) ◽  
pp. 233-238 ◽  
Author(s):  
Matthias Flowaczny ◽  
Albert Mehl ◽  
Karl-Heinz Kunzelmann ◽  
Reinhard Hickel
Keyword(s):  
Laser Scanning ◽  
Three Dimensional ◽  
Scanning Device

scholarly journals Methods used in the CRC program for the determination of carcass yield and beef quality

2001 ◽  
Vol 41 (7) ◽  
pp. 953 ◽  
Author(s):  
D. Perry ◽  
W. R. Shorthose ◽  
D. M. Ferguson ◽  
J. M. Thompson
Keyword(s):  
Meat Quality ◽  
Quality Data ◽  
Research Centre ◽  
Crossbred Cattle ◽  
Cooperative Research ◽  
Beef Quality ◽  
Carcass Yield ◽  
Core Program

This paper describes the methodology used for the collection of carcass yield and meat quality data from straightbred and crossbred cattle in the Cooperative Research Centre for Cattle and Beef Quality core program.

Integrated approach combining genetics, genomics and muscle biology to manage beef quality

2003 ◽  
Vol 2003 ◽  
pp. 52-52 ◽  
Author(s):  
I. Cassar-Malek ◽  
K. Sudre ◽  
J. Bouley ◽  
A. Listrat ◽  
Y. Ueda ◽  
...  
Keyword(s):  
Meat Quality ◽  
Muscle Growth ◽  
Genetic Selection ◽  
Integrated Approach ◽  
Nucleotide Polymorphisms ◽  
Beef Quality ◽  
Single Nucleotide ◽  
Biochemical Characteristics ◽  
Muscle Biology

Up to now, genetic selection in cattle has been directed in favour of muscle growth, which changes muscle characteristics, and hence meat quality. One key concern, that now needs examination, is to understand the relationships between muscle growth and muscle characteristics related to meat quality. To achieve such a goal, muscles of divergently selected animals were analysed by three complementary approaches: (i) determination of muscle biochemical characteristics, (ii) identification of differentially expressed genes using transcriptomic and proteomic tools, (iii) identification of Single Nucleotide Polymorphisms (SNP) within candidate genes.

A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

1987 ◽  
Vol 26 (01) ◽  
pp. 1-6 ◽  
Author(s):  
S. Selvaraj ◽  
M. R. Suresh ◽  
G. McLean ◽  
D. Willans ◽  
C. Turner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
T Antigen ◽  
Human Carcinomas ◽  
Animal Tumor ◽  
Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

Development and Pharmacokinetics Study of Antifungal Peptide Nanoliposomes by Liquid Chromatography-tandem Mass Spectrometry

2019 ◽  
Vol 15 (4) ◽  
pp. 312-318
Author(s):  
Shuoye Yang
Keyword(s):  
Mass Spectrometry ◽  
Biological Properties ◽  
Pharmacokinetic Property ◽  
Antifungal Peptide ◽  
Simple Liquid ◽  
Physico Chemical ◽  
Liquid Chromatography Tandem Mass ◽  
Pegylated Lipids

Background: The therapeutic ability and application of antifungal peptide (APs) are limited by their physico-chemical and biological properties, the nano-liposomal encapsulation would improve the in vivo circulation and stability. </P><P> Objective: To develop a long-circulating liposomal delivery systems encapsulated APs-CGA-N12 with PEGylated lipids and cholesterol, and investigated through in vivo pharmacokinetics. Methods: The liposomes were prepared and characterized, a rapid and simple liquid chromatographytandem mass spectrometry (LC-MS/MS) assay was developed for the determination of antifungal peptide in vivo, the pharmacokinetic characteristics of APs liposomes were evaluated in rats. Results: Liposomes had a large, unilamellar structure, particle size and Zeta potential ranged from 160 to 185 nm and -0.55 to 1.1 mV, respectively. The results indicated that the plasma concentration of peptides in reference solutions rapidly declined after intravenous administration, whereas the liposomeencapsulated ones showed slower elimination. The AUC(0-∞) was increased by 3.0-fold in liposomes in comparison with standard solution (20 mg·kg-1), the half-life (T1/2) was 1.6- and 1.5-fold higher compared to the reference groups of 20 and 40 mg·kg-1, respectively. Conclusion: Therefore, it could be concluded that liposomal encapsulation effectively improved the bioavailability and pharmacokinetic property of antifungal peptides.

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