98 Buttiauxella Phytase Improves Growth Performance of Weanling Pigs Fed Corn, Soybean Meal, and Canola Meal Based Diets

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 49-50
Author(s):  
Carly M Rundle ◽  
Yueming Dersjant-Li ◽  
Barthold Hillen ◽  
Maryane S Sespere Faria Oliveira ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that a Buttiauxella phytase expressed in T. reesei can compensate the reduction in standardized total tract digestible (STTD) P, Ca, digestible energy (DE), and standardized ileal digestible (SID) AA in diets for pigs without impaired growth performance. A positive control diet (PC) was formulated to meet the requirement for STTD P and total Ca (0.33% STTD P and 0.60% Ca). A negative control diet (NC) was formulated with 0.18% STTD P and 0.45% Ca and with reduction of DE by 43 kcal/kg and SID AA by 0.01–0.02% vs. PC. Adding 250, 500, or 1,000 phytase units (FTU) per kg to the NC diet for a total of 5 experimental diets formulated three additional diets. A total of 155 pigs (BW: 8.77 ± 1.38 kg) were allotted to the 5 treatments, and there were 8 replicate pens per treatment. The experiment was conducted for 21 d. Pigs were weighed at the beginning and conclusion of the experiment, and daily feed allotments were recorded. Average daily gain (ADG), average daily feed intake (ADFI), and gain: feed ratio (G:F) were calculated for each treatment. Data were analyzed using contrast statements to compare PC and NC, PC and phytase, and to determine linear and quadratic effects of phytase. Results indicated that pigs fed the NC diet had reduced (P < 0.05) ADG and G:F compared with pigs fed the PC diet (Table 1). Adding phytase to NC increased ADFI (linear, P < 0.05) and ADG (quadratic, P < 0.05) with the greatest values for the 1,000 FTU treatment. Including phytase in the diet also resulted in a quadratic increase (P < 0.05) in G:F. In conclusion, Buttiauxella phytase may be included in diets with reduced nutrients and energy for weanling pigs without compromising pig growth performance.

2017 ◽  
Vol 60 (No. 12) ◽  
pp. 675-682
Author(s):  
S. Mohana Devi ◽  
SC Kim ◽  
IH Kim

A total of 120 weanling pigs [(Landrace &times; Yorkshire) &times; Duroc], 21 days of age with an average initial body weight (BW) of 6.52 &plusmn; 0.22 (SE) kg were selected to investigate the effects of extruded rice product as a replacement for dried whey on growth performance, coefficient of total tract apparent digestibility (CATTD), blood profiles, faecal shedding of Lactobacillus and Escherichia coli (the microbial counts of digesta were expressed as log<sub>10</sub> colony forming units per gram), and faecal scores of weanling pigs. Pigs were randomly allocated to one of four dietary treatments, with six replicates per treatment and five pigs per pen. Pigs in the control group were fed a diet based on corn, soybean, and 20% dried whey. Experimental groups received the same diet as the control group, but dried whey was replaced by 3%, 6%, and 9% extruded rice. Throughout the experimental period (six weeks), no differences were observed in the average daily gain, average daily feed intake, and gain/feed ratio. Pigs fed 6% and 9% extruded rice had a lower (P &lt; 0.05) CATTD than pigs fed the control diet on Day 14. The blood creatinine concentration of pigs fed 9% extruded rice was higher (P &lt; 0.05) than that of pigs in the control group on Day 14. Pigs fed the diets containing 6% and 9% extruded rice had decreased (P &lt; 0.05) faecal E. coli counts on Day 14 compared with pigs fed the control diet. The current results indicate that feeding extruded rice can decrease faecal E. coli counts without negative effects on growth performance in weanling pigs.


2019 ◽  
Vol 98 (1) ◽  
Author(s):  
Gloria A Casas ◽  
Laia Blavi ◽  
Tzu-Wen L Cross ◽  
Anne H Lee ◽  
Kelly S Swanson ◽  
...  

Abstract An experiment was conducted to test the hypothesis that inclusion of the direct fed microbial Clostridium butyricum in diets for weanling pigs will improve growth performance, systemic immune function, microbiota composition, and gut morphology in weaned pigs. A total of 275 newly weaned pigs (20 ± 2 d of age) with an average initial BW of 6.4 ± 0.8 kg were allotted to a randomized complete block design with 11 pens per treatment. Diets included a positive control diet containing Carbadox, a negative control diet without Carbadox, and three treatment diets in which 1,250 × 108 cfu/kg, 2,500 × 108 cfu/kg, or 3,500 × 108 cfu/kg of C. butyricum was added to the negative control diet. A two-phase feeding program was used (phase 1, 14 d; phase 2, 21 d). At the conclusion of the experiment (day 35), a blood sample was collected from one pig per pen (11 pigs per treatment) and this pig was then euthanized and digesta and tissues samples were collected. Results indicated that for the overall phase, pigs fed the positive control diet had greater (P &lt; 0.05) ADG and ADFI and tended (P = 0.064) to have greater final BW than pigs fed the negative control diet. The ADG and G:F increased and then decreased as increasing doses of C. butyricum were included in the diet (quadratic, P &lt; 0.05). The concentration of tumor necrosis factor-α was less (P &lt; 0.05) in pigs fed the positive control diet compared with pigs fed the negative control diet or diets containing C. butyricum. Crypt depth tended (P = 0.08) to be less in pigs fed the negative control diet compared with pigs fed the positive control diet and villus height tended to increase as the doses of C. butyricum increased in the diets (quadratic, P = 0.08). Villus height also tended (P = 0.084) to be greater in pigs fed diets containing C. butyricum compared with pigs fed the positive control diet. Crypt depth increased as the dose of C. butyricum increased (quadratic, P &lt; 0.05) and villus width at the bottom tended to increase (linear, P = 0.072) as the dose of C. butyricum increased in the diet. Alpha and beta diversity indices of ileal and colonic microbiota were not affected by diet. In conclusion, addition of 1,250 × 108 cfu/kg of C. butyricum, but not greater levels, to diets fed to weanling pigs increased growth performance and tended to increase villus height and crypt depth, but changes in the abundance of intestinal microbiota were not observed.


2019 ◽  
Vol 99 (1) ◽  
pp. 66-73 ◽  
Author(s):  
Jin Ho Cho ◽  
Shu Dong Liu ◽  
Won Yun ◽  
Kwang Sik Kim ◽  
In Ho Kim

A total of 125 crossbred pigs with an initial body weight (BW) of 7.66 ± 1.30 kg were randomly distributed into one of five dietary treatments. Each treatment had five replicate pens with five pigs per pen. Treatments were as followed: (1) CON, control diet; (2) zinc oxide (ZO), CON + 0.3% ZO; (3) ZOM, CON + 0.3% ZO + 0.1% microencapsulated organic acids and pure botanicals (MOP); (4) microencapsulated zinc oxide (MZO), CON + 0.03% MZO; and (5) MZOM, CON + 0.03% MZO + 0.1% MOP. The MZO group had higher (P = 0.03) final BW than ZO group and also had higher (P = 0.04, 0.01) average daily gain and gain/feed ratio compared with ZO group during days 15–34 and throughout the experimental period. The MZO and MZOM had higher (P < 0.05) apparent total tract digestibility (ATTD) of dry matter (DM) and energy than ZO. Less (P < 0.05) feces Escherichia coli shedding was observed in the ZOM and MZOM diets compared with CON; the MZO had lowered (P = 0.001) zinc level in feces than ZO and CON. In conclusion, results indicated that supplementation with 0.3% MZO could improve the growth performance and ATTD of DM; inclusion of 0.1% MOP could decrease feces E. coli shedding and also decrease the zinc level in feces in weanling pigs.


2016 ◽  
Vol 96 (2) ◽  
pp. 243-251 ◽  
Author(s):  
H. Lu ◽  
A. Preynat ◽  
V. Legrand-Defretin ◽  
P.A. Geraert ◽  
O. Adeola ◽  
...  

Effect of carbohydrases and phytase supplementation on growth performance, nutrient utilization and gut health of nursery pigs was evaluated. Pigs were blocked by body weight (BW) and sex and allocated to four treatments. Treatments were a positive control (PC), a negative control (NC) deficient in metabolizable energy (ME), crude protein (CP), Ca, and non-phytate P (nPP), NC plus Rovabio® Max AP enzyme mix, at 0.05 and 0.075 g kg−1. Apparent total tract digestibility (ATTD) was determined in faecal samples. Apparent ileal digestibility (AID) was determined in ileal digesta samples collected after euthanasia. Lower final BW and average daily gain (ADG) (P < 0.05) were observed in NC compared with PC. Enzyme at 0.05 g kg−1increased (P < 0.05) BW on d 14 and d 41, respectively, and also increased ADG. Enzyme at 0.075 g kg−1increased BW on d 14 and ADG on d 0 to d 14 (P < 0.05). Feed efficiency [gain to feed ratio (G:F)] was greater (P < 0.05) in PC than NC from d 15 to d 41 and from d 0 to d 41. No difference in G:F was observed with enzyme supplementation. Higher (P < 0.05) serum Ca and bone ash were observed in PC than NC. Enzyme increased the ATTD of Ca and P (P < 0.05) compared with NC.


2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Zhong-Xing Rao ◽  
Mike D Tokach ◽  
Steve S Dritz ◽  
Jason C Woodworth ◽  
Joel M DeRouchey ◽  
...  

Abstract Two experiments were conducted to determine the efficacy of various commercial products on growth performance of nursery pigs fed diets high in fumonisin. In experiment 1, 350 pigs (241 × 600; DNA, Columbus, NE; initially 9.9 kg) were used with five pigs per pen and 14 replicates per treatment. After weaning, pigs were fed common diets for 21 d before the experiment started. The five dietary treatments consisted of a positive control (low fumonisin), a negative control (60 mg/kg of fumonisin B1 + B2 in complete diet), and the negative control with one of three products (0.3% of Kallsil Dry, Kemin Industries Inc., Des Moines, IA; 0.3% of Feed Aid Wide Spectrum, NutriQuest, Mason City, IA; 0.17% of Biofix Select Pro, Biomin America Inc., Overland Park, KS). Diets were fed in mash form for 14 d and followed with a low fumonisin diet for 13 d. For the 14-d treatment period, pigs fed the positive control diet and Biofix Select Pro had greater (P &lt; 0.05) average daily gain (ADG), average daily feed intake (ADFI), and gain:feed (G:F) compared to those fed the high fumonisin negative control, or high fumonisin diets with Kallsil Dry or Feed Aid Wide Spectrum. Serum sphinganine to sphingosine ratios (SA:SO) were greater (P &lt; 0.05) in all pigs fed high fumonisin diets compared to the positive control. In experiment 2, 300 pigs (241 × 600; DNA; initially 10.4 kg) were used. Procedures were similar to experiment 1 except there were 12 replicate pens per treatment, high fumonisin diets contained 30 mg/kg fumonisin, and experimental diets were fed for 28 d. Similar to experiment 1, pigs fed the positive control diet and treatment with Biofix Select Pro had greater (P &lt; 0.05) ADG and G:F, and lower (P &lt; 0.05) serum SA:SO compared to pigs fed the high fumonisin negative control, or high fumonisin diets with Kallsil Dry or Feed Aid Wide Spectrum. In summary, pigs fed diets containing 60 mg/kg of fumonisin for 14 d or 30 mg/kg of fumonisin for 28 d had poorer ADG and G:F and greater serum SA:SO compared to pigs fed a diet with less than 5 mg/kg of fumonisin. Adding Biofix Select Pro to diets appeared to mitigate the negative effects of high fumonisin concentrations, while Kallsil Dry and Feed Aid Wide Spectrum did not.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 100-101
Author(s):  
Carly M Rundle ◽  
Barthold Hillen ◽  
Yueming Dersjant-Li ◽  
Anne-Marie Debicki-Garnier ◽  
Hans H Stein

Abstract An experiment was conducted to compare two sources of phytase, Buttiauxella phytase expressed in T. reesei (Phytase B) and a hybrid phytase expressed in A. niger (Phytase H), on the apparent total tract digestibility (ATTD) of phosphorous (P) and calcium (Ca) in pigs fed diets without inorganic P. Eighty barrows (12.8 ± 1.04 kg) were placed in metabolism crates and fed 8 diets (10 pigs/diet). A positive control diet (PC) based on corn, wheat, canola meal, and soybean meal was formulated to contain 0.29% standardized total tract digestible (STTD) P and 0.60% Ca. A negative control (NC) was formulated without inorganic P, and this diet contained 0.48% Ca and 0.19% STTD P. Six additional diets were formulated by adding 250, 500, or 1000 units of each phytase (FTU)/kg to NC. After 5 d of adaptation, feces were collected from the feed fed during the following 4 days. Data were analyzed using contrast statements to compare source of phytase and determine the linear and quadratic effects within each source of phytase. With increasing dosage, fecal excretion of P decreased with phytase B (quadratic, P &lt; 0.05) and tended to decrease with phytase H (P &lt; 0.10), but fecal P was greater (P &lt; 0.05) for phytase H than phytase B. The ATTD of P increased quadratically with increasing dose of either phytase B or phytase H (P &lt; 0.05). Across dose levels, phytase B had greater (P &lt; 0.05) ATTD of P and Ca (77.3 and 83.5%) than phytase H (71.0 and 79.3), indicating that phytase B increases the ATTD of P and Ca to a greater extent than phytase H. In conclusion, both phytase B and phytase H, to a different extent, reduced fecal P excretion and increased ATTD of Ca and P in a dose dependent manner.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 400-401
Author(s):  
Charmaine D Espinosa ◽  
Deepak Velayudhan ◽  
Yueming Dersjant-Li ◽  
Janet Remus ◽  
Hans-Henrik H Stein

Abstract An experiment was conducted to test the hypothesis that increasing levels of phytase increases mineral digestibility and growth performance of pigs fed diets with 3,000 mg/kg of Zn. A positive control (PC) diet that met nutrient requirements and a negative control (NC) diet that was deficient in total Ca (-0.16%), digestible P (-0.14%), metabolizable energy (-50 kcal/kg), and digestible amino acids (-0.02%) were formulated. Eight additional diets were formulated by adding 500, 1,000, 1,500, or 2,000 phytase units/kg of a novel bacterial 6-phytase (PhyG) or a commercial Buttiauxella phytase (PhyB) to the NC diet. A randomized complete block design with 400 weanling pigs (5.82 ± 0.70 kg), 10 diets, 4 pigs per pen, and 10 replicate pens per diet was used. Pigs were fed the experimental diets for 28 d. Fecal samples were collected from 1 pig per pen on d 26 to 28. Linear and quadratic effects of PhyG and PhyB were determined using polynomial contrast statements. Contrast statements were used to compare effects of PC diet with NC diet, and to compare effects of PhyG diets with PhyB diets. Overall, pigs fed the NC diet had reduced growth performance (P &lt; 0.05) compared with pigs fed the NC diet (Table 1). Linear (P &lt; 0.01) and quadratic (P &lt; 0.05) increases in overall average daily gain and average daily feed intake of pigs were observed as dietary concentrations of PhyG or PhyB increased. Increasing levels of PhyG or PhyB linearly increased (P &lt; 0.01) the apparent total tract digestibility (ATTD) of P and Ca. PhyG increased ATTD of P more (P &lt; 0.05) than PhyB. In conclusion, both phytases are effective in increasing mineral digestibility and growth performance in pigs fed diets with pharmacological levels of Zn; however, PhyG improved P digestibility more than PhyB.


Author(s):  
S. Gilani ◽  
M.I. Garcia ◽  
L. Barnard ◽  
Y. Dersjant-Li ◽  
C. Millán ◽  
...  

The following study evaluated effects of a xylanase and beta-glucanase combination on growth performance of broilers fed energy reduced versus nutritionally adequate maize-soybean meal-based diets. A total of 648, one-day-old male broilers (Ross 308) were assigned to floor-pens (24 birds/pen, nine pens/treatment, three treatments) in a randomised block design. Treatments included: (1) a nutritionally adequate positive control diet (PC); (2) a negative control (NC) diet in which energy, crude protein and digestible amino acids were reduced by 3.4% (-105 kcal apparent metabolisable energy), 2.3% and 1.2 to 3.0% vs PC, respectively; and (3) NC plus a xylanase and beta-glucanase combination that supplied 1,220 U xylanase and 152 U beta-glucanase per kilogram of final feed. All diets contained a background of 500 FTU/kg phytase and were offered to birds ad libitum. Birds fed NC showed reduced average daily gain (ADG) by -6.1% (P<0.05); increased feed conversion ratio (FCR) by 9.2 points (P<0.05), and overall (d 1-35) body weight corrected FCR which was increased by 9.4 points (P<0.05) vs the PC group. Enzyme supplementation increased final BW (+4.2%, P<0.05), ADG (+5.4%, P<0.05) and tended to reduce FCR (+7.5 points, P=0.054) from d 22-35 vs NC, without affecting average daily feed intake. Improvements in performance due to the enzyme combination were equivalent to performance on the PC diet in all cases. The results suggested that significant improvements in growth performance of broilers fed maize-soybean meal-based diets which had been reduced in energy and nutrients can be realised by supplementation with xylanase in combination with beta-glucanase.


2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Dalton Humphrey ◽  
Spenser Becker ◽  
Jason Lee ◽  
Keith Haydon ◽  
Laura Greiner

Abstract Four hundred and eighty (PIC 337 X 1050, PIC Genus, Hendersonville, TN) pigs were used to evaluate a novel threonine source (ThrPro, CJ America Bio, Fort Dodge, IA) for nursery pigs from approximately 7 to 20 kg body weight (BW). After weaning, pigs were sorted by sex and fed a common diet for 1 wk. Upon completion of the first week, pigs were sorted into randomized complete blocks, equalized by weight, within 16 replications. Pigs were allocated to one of three dietary treatments: positive control (POS)—standard ileal digestible threonine-to-lysine ratio (SID; Thr:Lys) 0.60, negative control (NEG)—SID Thr:Lys ≤0.46, and alternative Thr source (TEST)—SID Thr:Lys 0.60. The alternative Thr source included fermentative biomass and was assumed to contain 75% Thr and a digestibility coefficient of 100% based on the manufacturer’s specifications. All other nutrients met or exceeded the NRC recommendations. Growth and intake data were analyzed as repeated measures with a compound symmetry covariance structure using the MIXED procedure in SAS 9.4 (SAS Institute Inc., Cary, NC) with pen as the experimental unit. Treatment, phase, the interaction between treatment and phase, and block were included as fixed effects in the model. Differences in total removals were tested using Fisher’s Exact Test of PROC FREQ. Results were considered significant at P ≤ 0.05 and considered a trend at P &gt; 0.05 and P ≤ 0.10. During the first 14 d, pigs fed TEST had decreased gain-to-feed ratio (G:F; 0.77 vs. 0.80, P = 0.022) compared to POS and increased G:F (0.77 vs. 0.73, P &lt; 0.001) compared to NEG. Over days 14–28, pigs fed TEST had similar G:F (0.71 vs. 0.70, P = 0.112) compared to POS and increased G:F (0.71 vs. 0.63, P &lt; 0.001) compared to NEG. Overall (days 0–28), pigs fed TEST had similar average daily gain (ADG; 0.47 vs. 0.47 kg/d, P = 0.982) and G:F (0.76 vs. 0.74, P = 0.395) compared to POS and increased ADG (0.47 vs. 0.43 kg/d, P &lt; 0.001) and G:F (0.76 vs. 0.67, P &lt; 0.001) compared to NEG. The average daily feed intake was not significantly different across treatments for the entirety of the study. In conclusion, the replacement of crystalline L-Thr with a novel Thr source resulted in similar growth performance in nursery pigs from approximately 7 to 20 kg.


1990 ◽  
Vol 38 (3B) ◽  
pp. 609-622
Author(s):  
N.P. Lenis ◽  
J.T.M. van Diepen

Individual and group housed crossbred pigs 45 to 105 kg and 65 to 95 kg in experiments 1 and 2, respectively, were given basal diets with L-threonine 0.6, 1.2 and 1.8 g/kg. Positive and negative control diets contained total threonine 5.7 and 4.5 g/kg, respectively. To prevent other amino acids being limiting, the negative control diet was supplemented with lysine, methionine, tryptophan, isoleucine, histidine and valine. The positive control diet was supplemented with lysine and methionine. The requirement for total threonine of growing-finishing pigs for maximum growth performance was about 5.6 g/kg in a diet containing net energy 9.4 MJ/kg. This figure corresponds with about 4.7 g/kg apparent faecal digestible threonine and 4.3 apparent ileal digestible threonine. There was no difference between the growing and the finishing pigs. The requirement for ileal digestible threonine, relative to ileal digestible lysine requirement, was about 64%. It is concluded that dietary protein can be reduced by 2 percentage units without any adverse effect on growth performance, if limiting amino acids are sufficiently supplemented. (Abstract retrieved from CAB Abstracts by CABI’s permission)


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