209 The Effect of Waiting Time at the Lairage of Slaughterhouse of Holstein Bulls After a Short Transport on Animal Behavior, Carcass Quality and Meat Quality

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 110-111
Author(s):  
Denise Sanchez ◽  
Joel Gonzalez ◽  
Guillem de Planell ◽  
Eliseu Isla ◽  
Sara Bover ◽  
...  
Keyword(s):  
Meat Quality ◽  
Waiting Time ◽  
Animal Behavior ◽  
Mixed Models ◽  
Repeated Measures ◽  
Lumbar Vertebra ◽  
Color Stability ◽  
Instrumental Color ◽  
Holstein Bulls ◽  
Meat Ph

Abstract The aim of this study was to evaluate the effect of waiting time at the lairage before slaughter in Holstein bulls after a short transport. Half of the bulls of each transport (n = 8 transports; n = 48 ± 4 bulls/transport) were distributed in two treatments: short waiting time (SWT; < 2h; n = 200) and long waiting time (LWT; from 4 to 8h; n = 184). Behavior of LWT animals was observed with a 15 min scan sampling at the lairage pens. Hot carcass weight (HCW), conformation, fatness and pH in Longissimus dorsi (LD) at the 4th-5th lumbar vertebra between 30 and 36 h postmortem was registered. A caudal section of LD was collected for meat quality evaluation in a subset of 24 animals selected based on HCW and meat pH. Each loin was cut in 5 slices, one to determine instrumental color after 30 min blooming, and the others were disposed to evaluate microbiology (1, 5, 8 and 12 d) and color stability (1 to 12 d). Data were analyzed using mixed models with repeated measures. The percentage of animals standing with LWT treatment decreased (P < 0.001) with waiting time (100% and 64%, 1 h and 6 h, respectively). Meat pH was greater (P < 0.01) for SWT (5.6 ± 0.02) compared with LWT (5.5 ± 0.02); however, the percentage of carcass classified as DFD (pH > 5.7) did not differ between treatments. Lightness (L*) was greater (P < 0.05) for SWT (29.7 ± 0.47) compared with LWT (28.1 ± 0.49). Moreover, on day 12 color stability and enterobacteria count evolved differently (P = 0.09) between treatments. Waiting time below 2 h might impair meat quality as animals might not be able to recover from transport.

Impact of Retail Case Type and Lighting Intensity on Instrumental Color Traits of Ground Beef Patties

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 47-47
Author(s):  
Mesa B Kutz ◽  
Kelly R Vierck ◽  
Janeal W S Yancey
Keyword(s):  
Repeated Measures ◽  
Fixed Effects ◽  
Ground Beef ◽  
Color Stability ◽  
Instrumental Color ◽  
Beef Patties ◽  
Color Traits ◽  
Hue Angle ◽  
Lighting Intensity ◽  
Case Type

Abstract The objective of this study was to determine the influence of retail case type and lighting intensity on color stability of ground beef patties. Ground 80:20 beef was procured from a local retail establishment between 7 and 10 d from the box date and ground through a 9.5 mm grinder plate, then formed into 150.25 g patties. Patties were then individually packaged in PVC-overwrap packages and randomly assigned into one of four treatments: open, multideck cases with 3000 K lighting (OPEN3000), open, 3500 K lighting (OPEN3500), enclosed, multideck cases with doors with 3000 K lighting (DOOR3000), and enclosed multideck at 3500 K lighting (DOOR3500). Patties were displayed for 7 d and instrumental color values (CIE L*, a*, and b*) were taken every 24 h. Data were analyzed as a 2 × 2 factorial arrangement with repeated measures, with case type, lighting intensity, and their interaction serving as fixed effects. Patties in DOOR cases possessed greater L* values (P < 0.05) than OPEN cases on d 3 of display. A similar trend existed for a*, where DOOR patties were redder (P < 0.05) than OPEN patties from d 2 to the end of display, and b* values were higher (P < 0.05) in DOOR cases from d 1 to d 4 of display. Patties from the DOOR cases also possessed increased chroma values (P < 0.05) from d 1 to 6 of display, and decreased (indicating more red) hue angle values from d 3 to the end of display. No differences were observed between lighting intensity for any trait evaluated (P ≥ 0.293). These results indicate patties placed in enclosed retail cases with doors are lighter, redder, and more intense in color during display compared to traditional open-front cases, which indicates greater color stability.

scholarly journals Clinical characterization of dysautonomia in long COVID-19 patients

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicolas Barizien ◽  
Morgan Le Guen ◽  
Stéphanie Russel ◽  
Pauline Touche ◽  
Florent Huang ◽  
...  
Keyword(s):  
Mixed Models ◽  
Repeated Measures ◽  
Physical Medicine ◽  
Persistent Symptoms ◽  
Index Changes ◽  
Changes Over Time ◽  
And Control ◽  
Testing Group ◽  
Over Time

AbstractIncreasing numbers of COVID-19 patients, continue to experience symptoms months after recovering from mild cases of COVID-19. Amongst these symptoms, several are related to neurological manifestations, including fatigue, anosmia, hypogeusia, headaches and hypoxia. However, the involvement of the autonomic nervous system, expressed by a dysautonomia, which can aggregate all these neurological symptoms has not been prominently reported. Here, we hypothesize that dysautonomia, could occur in secondary COVID-19 infection, also referred to as “long COVID” infection. 39 participants were included from December 2020 to January 2021 for assessment by the Department of physical medicine to enhance their physical capabilities: 12 participants with COVID-19 diagnosis and fatigue, 15 participants with COVID-19 diagnosis without fatigue and 12 control participants without COVID-19 diagnosis and without fatigue. Heart rate variability (HRV) during a change in position is commonly measured to diagnose autonomic dysregulation. In this cohort, to reflect HRV, parasympathetic/sympathetic balance was estimated using the NOL index, a multiparameter artificial intelligence-driven index calculated from extracted physiological signals by the PMD-200 pain monitoring system. Repeated-measures mixed-models testing group effect were performed to analyze NOL index changes over time between groups. A significant NOL index dissociation over time between long COVID-19 participants with fatigue and control participants was observed (p = 0.046). A trend towards significant NOL index dissociation over time was observed between long COVID-19 participants without fatigue and control participants (p = 0.109). No difference over time was observed between the two groups of long COVID-19 participants (p = 0.904). Long COVID-19 participants with fatigue may exhibit a dysautonomia characterized by dysregulation of the HRV, that is reflected by the NOL index measurements, compared to control participants. Dysautonomia may explain the persistent symptoms observed in long COVID-19 patients, such as fatigue and hypoxia. Trial registration: The study was approved by the Foch IRB: IRB00012437 (Approval Number: 20-12-02) on December 16, 2020.

Changes in multiple sclerosis symptoms are associated with changes in work productivity of people living with multiple sclerosis

2021 ◽  
pp. 135245852199455
Author(s):  
Barnabas Bessing ◽  
Mohammad A Hussain ◽  
Suzi B Claflin ◽  
Jing Chen ◽  
Leigh Blizzard ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Mixed Models ◽  
Repeated Measures ◽  
Work Productivity ◽  
Annual Change ◽  
Individual Variations ◽  
Unit Increase ◽  
The Mean ◽  
Employment Rates ◽  
Study Participants

Background: While employment rates have increased in people with multiple sclerosis (PwMS), little is known about the longitudinal trends of work productivity. Objective: To describe the longitudinal patterns of work productivity and examine the factors associated with annual change of work productivity of PwMS. Methods: Study participants were employed participants of the Australian MS Longitudinal Study (AMSLS) followed from 2015 to 2019 with at least two repeated measures ( n = 2121). We used linear mixed models to examine if the within-individual variations in MS symptoms are associated with changes in work productivity. Results: The mean annual change in work productivity between 2015 and 2019 was −0.23% ( SD = 18.68%). Not the actual severity of symptoms but rather the changes in severity of symptoms that are associated with change in work productivity in the same year. In a multivariable model, every unit increase in mean annual change in ‘pain and sensory symptoms’, ‘feelings of anxiety and depression’, and ‘fatigue and cognitive symptoms’ were independently associated with 2.43%, 1.55% and 1.01% annual reductions in work productivity, respectively. Conclusion: Individual changes in work productivity are largely driven by the changes in symptom severity rather than the absolute severity. Stabilising/improving MS symptoms might improve work productivity.

scholarly journals Associations between Longitudinal Patterns of Substance Use and Anxiety and Depression Symptoms among a Sample of Canadian Secondary School Students

2021 ◽  
Vol 18 (19) ◽  
pp. 10468
Author(s):  
Gillian C. Williams ◽  
Karen A. Patte ◽  
Mark A. Ferro ◽  
Scott T. Leatherdale
Keyword(s):  
Substance Use ◽  
Mixed Models ◽  
Repeated Measures ◽  
Latent Class ◽  
Treatment Strategies ◽  
Anxiety And Depression ◽  
Depression Symptoms ◽  
Cigarette Use ◽  
School Students ◽  
Relevant Covariates

The objective of this study is to examine the longitudinal associations between latent classes of substance use and anxiety and depression scores among youth who use substances. This study uses data from three waves (Wave 1: 2017/18, Wave 2: 2018/19, and Wave 3: 2019/20) of the COMPASS study. Students in grades 9 and 10 who reported substance use at baseline (n = 738) report their substance use (alcohol, cannabis, cigarettes, and e-cigarettes) and anxiety and depression symptoms at each wave. A Repeated Measures Latent Class Analysis (RMLCA) is used to determine substance use classes, and mixed models are used to examine the associations between substance use classes and anxiety and depression. We identify three classes of substance use: (1) occasional alcohol and e-cigarette use, (2) escalating poly-substance use, and (3) consistent poly-substance use. After controlling for relevant covariates, consistent poly-substance use is associated with depression (Female OR: 1.24 [95%CI: 0.46, 2.02]; Male OR 1.13 [95%CI: 0.38, 1.87]) but not anxiety. Escalating poly-substance use is associated with depression among males (OR 0.72 [95%CI: 0.10, 1.33]). These findings should be taken into consideration when creating prevention programming and treatment strategies for adolescents. Substance use programming should be comprehensive, consider multiple substances, and be cognizant of symptoms of mental illness, particularly depression.

scholarly journals 1 Physiological and performance recovery after fourteen hours of feed restriction or transportation in Holstein bulls fed high concentrate diets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 2-3
Author(s):  
Sonia Marti ◽  
Elena Garcia ◽  
Christine Gerard ◽  
Joan Grau ◽  
Nicolas Cirier ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Feed Restriction ◽  
Amyloid A ◽  
Performance Recovery ◽  
Treatment Data ◽  
Access To Water ◽  
Holstein Calves ◽  
Holstein Bulls ◽  
And Performance ◽  
Beta Hydroxybutyrate

Abstract One hundred and eight Holstein calves (225 ± 1.1 kg and 187 ± 5.2 d) were used to evaluate the physiological and performance recovery after 14 h transportation or feed restriction. Calves were distributed into 6 pens (2 pens/treatment) according to control (CTR, n = 36) calves with ad libitum access to concentrate, straw and water; restricted (RES, n = 36) calves with concentrate restriction but with access to water and straw for 14 h; and transported (TRA, n = 36) calves that were loaded into a trailer and transported without feed or water for 14 h. On days 0, 7, 21, and 35 BW was recorded. Concentrate intake were recorded daily. Blood samples for non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), and serum amyloid-A (SAA) were collected at -24, -14, 0 h, and 6, 24, and 168 h post-treatment. Data were analyzed using mixed models with repeated measures. At 24 h, RES and TRA had greater (P < 0.05) concentrate intake compared with CTR. However, from d 7 to 35 after treatments, only TRA had similar concentrate intake than CTR, while RES had lesser (P < 0.05) concentrate intake than CTR and TRA. RES at 6 h had greater (P < 0.05) NEFA concentrations than TRA, and NEFA concentrations were still higher for the RES and TRA groups than those for the CTR after 24 h. After 24 h concentrations of serum BHBA for TRA and RES were significantly greater (P < 0.05) when compared with those for the CTR. Serum concentration of SAA for TRA and RES was greater (P < 0.05) than CTR until 168 h. Results showed similar effects of 14 h of feed restriction and transportation of calves on serum anorexia and inflammation parameters; however, feed intake was recovered after d 35 in transported calves but not in feed restricted calves without transportation.

scholarly journals Influence of feeding regime and finishing system on lamb muscle fiber and meat quality

2009 ◽  
Vol 38 (11) ◽  
pp. 2204-2210 ◽  
Author(s):  
Sarita Bonagurio Gallo ◽  
Edson Ramos de Siqueira ◽  
Eduardo Francisquine Delgado ◽  
Maeli Dal Pai Silva ◽  
Gilberto Teixeira da Rosa
Keyword(s):  
Meat Quality ◽  
Muscle Fiber ◽  
Live Weight ◽  
Nutritional Supplement ◽  
Fiber Types ◽  
Post Mortem ◽  
Fiber Morphology ◽  
Complete Diet ◽  
Weaning Age ◽  
Meat Ph

The study verified the influence of different nutritional levels of the dams, before parturition, the weaning age of the lambs and the finishing system on lamb muscle fiber morphology and meat quality. Ile-de-France × Bergamacia crossbred dams grazing on natural grassland were divided into two groups: 1) nutritional supplement 30 days before the parturition (SUPL), and 2) no nutritional supplement (NS). These groups were further subdivided by weaning age of the lambs, which corresponded to either 45 or 60 days. The weaned lambs from each of the previous groups were also divided into different finishing systems: confined and fed a complete diet (CD), confined and fed hay (H) and kept on grassland (P). The lambs were slaughtered at 30 kg live weight (CD and P), or at 150 days of age (H). The carcasses were cooled at 4ºC/24h. The analyzed variables were: longissimus dorsi muscle fiber morphophysiology, post mortem myofibrillar fragmentation (at 0, 3 and 7 days post mortem), meat pH and temperature. There was no effect of supplementation of dams or weaning age of the lambs for the evaluated variables. The frequency of slow-oxidative (SO), fast-oxidative-glycolytic (FOG) and fast-glycolytic (FG) muscle fiber types was not altered by the treatments, but the fiber cross-sectional area was smaller for the lambs finished only on hay, and the meat pH values and temperature were lower and the myofibrill fragment dimensions were smaller. As time maturation increased from 0 to 3 and 7 days post mortem, there was a decrease in the length of the myofibrill fragments. The diet of the lambs which were maintained confined and fed only with hay was nutritionally inferior when compared with all the others, and this changed the quality of the meat compared to the finishing systems on grassland or confined and fed complete diet.

scholarly journals Report Quality of Generalized Linear Mixed Models in Psychology: A Systematic Review

2021 ◽  
Vol 12 ◽  
Author(s):  
Roser Bono ◽  
Rafael Alarcón ◽  
María J. Blanca
Keyword(s):  
Systematic Review ◽  
Mixed Models ◽  
Repeated Measures ◽  
Web Of Science ◽  
Generalized Linear Mixed Models ◽  
Linear Mixed Models ◽  
Journal Citation Reports ◽  
Report Quality ◽  
Fixed And Random Effects

Generalized linear mixed models (GLMMs) estimate fixed and random effects and are especially useful when the dependent variable is binary, ordinal, count or quantitative but not normally distributed. They are also useful when the dependent variable involves repeated measures, since GLMMs can model autocorrelation. This study aimed to determine how and how often GLMMs are used in psychology and to summarize how the information about them is presented in published articles. Our focus in this respect was mainly on frequentist models. In order to review studies applying GLMMs in psychology we searched the Web of Science for articles published over the period 2014–2018. A total of 316 empirical articles were selected for trend study from 2014 to 2018. We then conducted a systematic review of 118 GLMM analyses from 80 empirical articles indexed in Journal Citation Reports during 2018 in order to evaluate report quality. Results showed that the use of GLMMs increased over time and that 86.4% of articles were published in first- or second-quartile journals. Although GLMMs have, in recent years, been increasingly used in psychology, most of the important information about them was not stated in the majority of articles. Report quality needs to be improved in line with current recommendations for the use of GLMMs.

scholarly journals Oestrogen levels in serum and urine of premenopausal women eating low and high amounts of meat

2013 ◽  
Vol 17 (9) ◽  
pp. 2087-2093 ◽  
Author(s):  
Brook E Harmon ◽  
Yukiko Morimoto ◽  
Fanchon Beckford ◽  
Adrian A Franke ◽  
Frank Z Stanczyk ◽  
...  
Keyword(s):  
Mixed Models ◽  
Repeated Measures ◽  
Premenopausal Women ◽  
Least Square ◽  
Serum Samples ◽  
Meat Eating ◽  
Three Samples ◽  
Blood And Urine Samples ◽  
The Mean ◽  
Serum And Urine

AbstractObjectiveBased on the hypothesis that high-meat diets may increase breast cancer risk through hormonal pathways, the present analysis compared oestrogens in serum and urine by meat-eating status.DesignIntervention with repeated measures.SettingTwo randomized soya trials (BEAN1 and BEAN2) among premenopausal healthy women.SubjectsBEAN1 participants completed seven unannounced 24 h dietary recalls and donated five blood and urine samples over 2 years. BEAN2 women provided seven recalls and three samples over 13 months. Serum samples were analysed for oestrone (E1) and oestradiol (E2) using RIA. Nine oestrogen metabolites were measured in urine by LC–MS. Semi-vegetarians included women who reported consuming <30 g of red meat, poultry and fish daily, and pescatarians those who reported consuming <20 g of meat/poultry but >10 g of fish daily. All other women were classified as non-vegetarians. We applied mixed models to compute least-square means by vegetarian status adjusted for potential confounders.ResultsThe mean age of the 272 participants was 41·9 (sd 4·5) years. Serum E1 (85 v. 100 pg/ml, P = 0·04) and E2 (140 v. 154 pg/ml, P = 0·04) levels were lower in the thirty-seven semi-vegetarians than in the 235 non-vegetarians. The sum of the nine urinary oestrogen metabolites (183 v. 200 pmol/mg creatinine, P = 0·27) and the proportions of individual oestrogens and pathways did not differ by meat-eating status. Restricting the models to the samples collected during the luteal phase strengthened the associations.ConclusionsGiven the limitations of the study, the lower levels of serum oestrogens in semi-vegetarians than non-vegetarians need confirmation in larger populations.

Abstract P263: The Associations of Epithelial Sodium Channel Genes with Blood Pressure Changes and Hypertension Incidence: the GenSalt Study

Circulation ◽  
2014 ◽  
Vol 129 (suppl_1) ◽  
Author(s):  
Tanika N Kelly ◽  
Xueli Yang ◽  
Dabeeru C Rao ◽  
Dongfeng Gu ◽  
James E Hixson ◽  
...  
Keyword(s):  
Mixed Models ◽  
Repeated Measures ◽  
Multiple Testing ◽  
Han Chinese ◽  
Time Interaction ◽  
False Discovery ◽  
Pressure Changes ◽  
Multiple Variants ◽  
Baseline Examination

We examined the associations of common variants in ENaC genes with BP changes and hypertension incidence in a longitudinal family study. A total of 2755 Han Chinese participants of the Genetic Epidemiology Network of Salt Sensitivity (GenSalt) baseline examination were eligible for the current study. The average of nine BP measurements obtained using a random-zero sphygmomanometer at the baseline and each of two follow-up visits were used for the current analysis. The associations of 43 tag-SNPs in ENaC genes with BP changes and hypertension incidence were assessed using mixed models to account for the correlations of repeated measures among individuals and within families. A genotype by time interaction term was used to model differences in BP change according to genotype over time. The false discovery rate (FDR) method was used to adjust for multiple testing. During an average of 7.4 years follow-up, systolic BP (SBP) and diastolic BP (DBP) increased, and approximately 33% of participants developed hypertension. Multiple variants in ENaC genes were significantly associated with longitudinal changes in SBP after adjustment for multiple testing, including SCNN1A SNP rs11064153 ( P interaction = 5.8х10 -4 ; FDR-Q = 0.01); SCNN1G SNPs rs4299163 ( P interaction = 0.011; FDR-Q = 0.05) and rs4401050 ( P interaction = 0.001; FDR-Q = 0.01); and SCNN1B SNP rs1004749 ( P interaction = 0.006; FDR-Q = 0.03). Similar but not significant trends were observed for the associations between both rs11064153 and rs4401050 and DBP changes ( P interaction = 0.024 and 0.005, respectively; FDR-Q = 0.41 and 0.17, respectively) and between rs11604153 and hypertension incidence ( P = 0.017; FDR-Q = 0.19). Our findings indicate that ENaC genes may contribute to longitudinal BP changes in the Han Chinese population. Replication of these findings is warranted.

scholarly journals 99 Effects of replacing monensin and tylosin in finishing cattle diets with essential oil and/or benzoic acid on meat color and oxidative stability of ribeye steaks and ground beef

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 56-56
Author(s):  
Lydia M Wang ◽  
Shiqi Huang ◽  
Sebastian Chalupa-Krebzdak ◽  
Sandra M Vasquez Mejia ◽  
Benjamin M Bohrer
Keyword(s):  
Benzoic Acid ◽  
Lipid Oxidation ◽  
Essential Oils ◽  
Repeated Measures ◽  
Block Design ◽  
Ground Beef ◽  
Thiobarbituric Acid ◽  
Finishing Cattle ◽  
Instrumental Color ◽  
Beef Products

Abstract This study examined the effects of replacing monensin and tylosin with essential oils and/or benzoic acid in finishing cattle diets on beef color stability and lipid oxidation during a simulated retail display period. The longissimus thoracis (LT) and semimembranosus (SM) muscles were obtained from 63 steers that were fed for 98 days on 1 of 5 finishing diets: no additional supplement (CON), monensin/tylosin (M/T), essential oils (EO), benzoic acid (BA), or a combination of essential oils and benzoic acid (COMBO). Instrumental color measured with a Minolta colorimeter and visual discoloration evaluated by two trained panelists were recorded daily for three beef products placed under simulated retail conditions, until a 60% surface discoloration was observed. The three beef products evaluated were LT steaks, and two types of ground beef patties manufactured with the SM muscles (lean – no additional fat and regular – 25% added fat). The thiobarbituric acid reactive substances (TBARS) assay was used before and after the shelf life display to determine the degree of lipid oxidation for products during the display. Data were analyzed as a randomized complete block design with the GLIMMIX procedure of SAS using repeated measures. Results indicated a treatment by day interaction (P < 0.0001) for visual discoloration and instrumental color of all three products. At the end of the display period, products from the COMBO diet had on average the least amount of visual discoloration and the greatest a* (redness) value, while the CON steaks and M/T ground beef had the most discoloration and the lowest a* values. TBARS values at d 0 and d 7 did not differ (P > 0.23) among treatments for all three products. Overall, supplemental ingredients in feed did not affect the color and degree of lipid oxidation of steaks and ground beef to a level deemed detrimental.

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