309 Nutrient Digestibility of Beef Steers Consuming Finishing Diets with an Added Nutritional Packet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 168-169
Author(s):  
Kaue T Tonelli Nardi ◽  
Jhones O Onorino Sarturi ◽  
Darren D Henry ◽  
Francine M Ciriaco ◽  
Nelson O Huerta-Leidenz ◽  
...  
Keyword(s):  
Block Design ◽  
Vitamin B1 ◽  
Nutrient Digestibility ◽  
Experimental Unit ◽  
Beef Steers ◽  
Yeast Saccharomyces Cerevisiae ◽  
Finishing Diets ◽  
Time Of Feeding ◽  
Internal Marker ◽  
Feed Samples

Abstract The effects of a nutritional packet fed to beef steers during the final 64 d of the feedlot finishing phase on apparent total tract nutrient digestibility were evaluated. Angus-crossbred steers (n =120; initial BW = 544 ± 52 kg) were assigned to 30 pens (4 steers/pen; 15 pens/treatment; pen = experimental unit) in a randomized complete block design. A steam-flaked corn-based finishing diet was fed ad libitum and treatments applied as follows: 1) control and 2) 30 g/steer-daily (DM-basis) of the nutritional packet [containing live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g), vitamin C (5.4 g/kg), vitamin B1 (13.33 g/kg), NaCl (80 g/kg) and KCl (80 g/kg)]. The nutritional packet used ground corn as a carrier and was included at 1% of diet DM. Orts were quantified daily (if any) and subtracted from the total dietary DM offered to calculate DM intake. Fecal samples were collected twice daily (0700 and 1700 h) for 5 consecutive days (d 47 to 51) from at least 3 steers within pen. Feed samples were collected daily at time of feeding (0630 h). Fecal and feed samples were composited to represent the collection period, dried (55oC), ground (1 mm), and analyzed to assess DM, OM, NDF, ADF, and hemicellulose. In situ (288 h) iNDF was used as an internal marker to measure apparent total tract nutrient digestibility. Data were analyzed using the GLIMMIX procedure of SAS. Intake of DM, OM, and fiber components during the digestibility period was not affected (P ≥ 0.44) by treatment. Steers fed the nutritional packet had increased apparent total tract digestibility of OM (P = 0.02), DM (P = 0.01), NDF (P = 0.02), ADF (P = 0.01), and hemicellulose (P = 0.08; tendency). Improved nutrient digestibility without affecting nutrient intake may warrant an enhanced energy deposition in the carcass.

315 Feeding Behavior of Beef Steers Consuming Finishing Diets with Added Nutritional Packet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 170-170
Author(s):  
Igor Gomes Fávero ◽  
Jhones O Onorino Sarturi ◽  
Kaue T Tonelli Nardi ◽  
Daren D Henry ◽  
Kaliu G Scaranto Silva ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Block Design ◽  
Vitamin B1 ◽  
Experimental Unit ◽  
Beef Steers ◽  
Yeast Saccharomyces Cerevisiae ◽  
Eating Time ◽  
Finishing Diets ◽  
D 63 ◽  
Randomized Complete Block Design

Abstract The effects of a nutritional packet fed to beef steers during the feedlot finishing phase (final 64 d) on feeding behavior were evaluated. Crossbred-Angus steers (n = 120; initial BW = 544 ± 52 kg) were assigned to 30 pens (4 steers/pen; 15 pens/treatment; pen = experimental unit) in a randomized complete block design. Steers were fed, ad libitum, a steam-flaked corn-based finishing diet and treatments were applied as follows: 1) control and 2) 30 g/steer-daily (DM-basis) of a nutritional packet (containing live yeast [Saccharomyces cerevisiae; 8.7 Log CFU/g], vitamin C [5.4 g/kg], vitamin B1 [13.33 g/kg], NaCl [80 g/kg], and KCl [80 g/kg]). The nutritional packet used ground corn as a carrier and was included at 1% of diet DM. Orts were subtracted from the total dietary DM offered to calculate DM intake. Feeding behavior activities were visually assessed by trained personnel twice (d 27 and d 63) for 24 h, when time spent on each activity was recorded every 5 min. Data were analyzed using the GLIMMIX procedure of SAS. Treatment × period interactions (P ≤ 0.03) were observed for eating time (min/d) and eating rates (min/kg of consumed DM, OM, fiber, and digestible DM, OM, and fiber), in which steers fed the nutritional packet spent less time in such activities on d 27 and more time during the assessment on d 63, compared with control. Regardless of period, steers fed the nutritional packet tended (P = 0.07) to spend less time chewing per kg of digestible ADF. Rumination (P ≥ 0.28), drinking (P ≥ 0.40), active (P ≥ 0.92), and resting (P ≥ 0.53) times were not affected by treatment. The nutritional packet affected eating behavior more intensively during the first 27 d of treatment, and digestible ADF seems to be the main feed-fraction inducing such effect.

PSVI-30 Feedlot performance and carcass characteristics of beef steers under intensive management with or without an added nutritional packet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 446-446
Author(s):  
Andrea M Osorio ◽  
Kaue Tonelli Nardi ◽  
Igor Gomes Fávero ◽  
Kaliu G Scaranto Silva ◽  
Kymberly D Coello ◽  
...  
Keyword(s):  
Block Design ◽  
Random Effect ◽  
Vitamin B1 ◽  
Carcass Characteristics ◽  
Quality Data ◽  
Experimental Unit ◽  
Beef Steers ◽  
Intensive Management ◽  
Feedlot Performance ◽  
Yeast Saccharomyces Cerevisiae

Abstract The effects of a nutritional packet on performance and carcass characteristics of feedlot beef steers were evaluated. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based finishing-diet, ad libitum, for the last 65-d on feed prior to slaughter, in which treatments were: 1) control and 2) a nutritional-packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Individual BW was recorded every 14 d and carcass quality data was collected at slaughter. Steer was considered the experimental unit. The MIXED procedure of SAS was used with the fixed effect of treatment and the random effect of block. Total DMI was unaffected (P = 0.610) by treatment from d 0–65; however, control steers had greater (P = 0.030) DMI from d 54–65. The G:F was unaffected (P = 0.830) by treatment from d 0–35; however, it was greater (P ≤ 0.001) for steers fed the nutritional packet from d 0–50, and 0–65. Treatment did not affect (P = 0.920) ADG from d 0–35, however it increased (P ≤ 0.001) ADG from d 0–50 and tended (P = 0.060) to increase ADG from d 0–65. Carcass adjusted final BW tended (P = 0.060) to increase by 28% for treated cattle, whereas the Longissimus dorsi area tended to decrease (P = 0.090). No differences (P = 0.240) were observed for fat thickness, while yield-grade (P = 0.03) increased for treated cattle. The nutritional packet appears to improve performance in the final days of feeding steers under intensive management, and energy partitioning may be altered to fat deposition.

310 Growth Performance and Carcass Characteristics of Beef Steers Consuming Finishing Diets with an Added Nutritional Packet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 169-169
Author(s):  
Kaue T Tonelli Nardi ◽  
Jhones O Onorino Sarturi ◽  
Darren D Henry ◽  
Francine M Ciriaco ◽  
Nelson O Huerta-Leidenz ◽  
...  
Keyword(s):  
Growth Performance ◽  
Block Design ◽  
Average Daily Gain ◽  
Vitamin B1 ◽  
Carcass Characteristics ◽  
Fat Deposition ◽  
Experimental Unit ◽  
Thiamine Hydrochloride ◽  
Beef Steers ◽  
Finishing Diets

Abstract The effects of a nutritional packet fed to beef steers during the final 64 d of the feedlot finishing phase on growth performance and carcass characteristics were evaluated. Crossbred-Angus steers (n = 120; initial BW = 544 ± 52 kg) were assigned to 30 pens (4 steers/pen; 15 pens/treatment) in a randomized complete block design where pen represented the experimental unit. A steam-flaked corn-based finishing diet was fed ad libitum, and treatments were applied as follows: 1) control and 2) 30 g/steer-daily (DM-basis) of the nutritional packet (containing live yeast [8.7 Log CFU of Saccharomyces cerevisiae], vitamin C [5.4 g/kg of Ascorbic acid], vitamin B1 [13.33 g/kg of Thiamine hydrochloride], sodium chloride [80 g/kg] and potassium chloride [80 g/kg]). The nutritional packet used ground corn as a carrier and was included at 1 % of diet DM. Orts were quantified daily (if any) and subtracted from the total dietary DM offered to calculate DM intake. Unshrunk BW were collected on d 0, 21, 42 and 64 before daily feeding at 0630 h. Data were analyzed using the GLIMMIX procedure of SAS. Average daily gain (P = 0.89), dry matter intake (P = 0.57) and, gain:feed (P = 0.66) were not affected by the inclusion of the nutritional packet. The 12th rib fat deposition was increased (P ≤ 0.02) for cattle fed the nutritional packet compared with control (15.70 vs. 13.36 mm, respectively). Consequently, calculated yield grade was also increased (P ≤ 0.03) for cattle consuming the nutritional packet (3.32 vs. 3.02). An improved carcass fat-deposition without any deleterious effects on growth performance potentially warrant less days on feed when cattle receive such nutritional packet.

305 Methane Emissions and Apparent Total Tract Nutrient Digestibility of Feedlot Beef Steers Under Intensive Management with or Without an Added Nutritional Packet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-167
Author(s):  
Andrea M Osorio ◽  
Kaue T Tonelli Nardi ◽  
Igor Gomes Fávero ◽  
Kaliu G Scaranto Silva ◽  
Kymberly D Coello ◽  
...  
Keyword(s):  
Treatment Period ◽  
Repeated Measures ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Methane Emissions ◽  
Nutrient Digestibility ◽  
Experimental Unit ◽  
Beef Steers ◽  
Ch4 Production

Abstract The effects of a nutritional packet were evaluated on CH4 emissions and apparent total tract nutrient digestibility of feedlot beef steers. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens equipped with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based diet (88% concentrate DM basis) ad libitum for the last 65 d on feed, and received the following treatments: 1) control and 2) a nutritional packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Methane emissions and apparent total tract nutrient digestibility were measured during 3 periods with 5-d of collections each. Gas emissions from steers were measured utilizing the SF6 tracer technique. Feed and fecal samples were collected once and twice (0700 h and 1600 h) daily, respectively, to determine digestibility of nutrients using iNDF as an internal marker. Steer was considered the experimental unit. Data were analyzed as repeated measures using the MIXED procedure of SAS with the fixed effects of treatment, period, and their interaction, and the random effect of block. No treatment × period interactions (P ≥ 0.125) were observed for DMI and any of the CH4 production variables (g/day, g/kg BW0.75, g/nutrient intake, and g/nutrient digested). Moreover, treatments did not affect digestibility of DM, OM, or ADF (P ≥ 0.300); however, digestibility of NDF was increased for treated cattle (P = 0.013), which resulted in a tendency (P = 0.098) to decrease CH4 production in g per kg NDF intake and decreased (P = 0.020) grams CH4 per kg NDF digested. The nutritional packet may be altering ruminal fermentation on intensively managed steers and improving fiber digestibility, which can have benefits on CH4 emission intensity.

scholarly journals 251 Evaluation of Condensed Algal Residue Solubles as an Ingredient in Cattle Finishing Diets

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 137-138
Author(s):  
Calvin Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Bradley M Boyd ◽  
Levi J McPhillips ◽  
...  
Keyword(s):  
Great Plains ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Distillers Grains ◽  
Experimental Unit ◽  
Omega 3 ◽  
Finishing Diets ◽  
Randomized Block Design ◽  
Algae Oil

Abstract Algae oil production for Omega-3 fatty acid supplementation yields a byproduct called Condensed Algal Residue Solubles (CARS; 25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), de-oiled algae cells with residual fermentation substrates. This study evaluated the use of CARS in feedlot finishing diets. Crossbreed steers, (n=480) were blocked and stratified by initial body weight (BW) into 4 blocks, and assigned randomly to treatments. Treatments were designed as a 2 x 3 factorial with 3 inclusions of CARS (0, 2.5, 5% of diet DM) and 2 different base diets representing Northern and Southern Great Plains diets. The Southern diets contained steam flaked corn and dry distillers grains while the Northern diets had dry rolled and high moisture corn with wet distillers grains. CARS replaced corn in both diets. All blocks were harvested after 148 days on feed. Performance data were analyzed as a randomized block design with CARS inclusion, base diet, and interactions as fixed effects, BW block as a random effect and pen (n=48) as the experimental unit. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant interactions between CARS inclusion and diet type (P ≥ 0.49). Main effects of CARS indicated positive quadratic responses for carcass adjusted ADG, G:F, 12th rib back fat, yield grade (P < 0.01; increasing to 2.5% inclusion, decreasing at 5%) and hot carcass weight was both linear and quadratic (P ≤ 0.01 and P ≥ 0.06 respectively; 969, 977, 935 as CARS increased). Linear decrease in DMI, final adjusted BW and ribeye area (P ≤ 0.01) as CARS increased. Cattle fed the Southern diets had greater ADG and G:F compared to Northern diets (P < 0.01). Including 2.5% CARS in the diet improved feed efficiency in both Northern and Southern based feedlot diets.

scholarly journals Effect of Dietary Symbiotic and Acidifier on Growth Performance and Nutrients Digestibility in Growing Pigs Crossbreed

2020 ◽  
Vol 6 (2) ◽  
pp. 51
Author(s):  
Danung Nur Adli ◽  
Osfar Sjofjan
Keyword(s):  
Growth Performance ◽  
Block Design ◽  
Basal Diet ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Experimental Unit ◽  
Randomized Complete Block Design ◽  
Dietary Treatments ◽  
Nutrients Digestibility

The aim of this study was to assess the effects of dietary probiotic enhanced liquid acidifier combined with mannan-rich fraction on growth performance, nutrients digestibility in growing pigs. Thirty [(Duroc×Yorkshire)×Landrace] pigs with the average initial BW of 36.75±1.57 kg were allocated into three treatments by a randomized complete block design. There were five pens per treatment with six pigs per pen. Dietary treatments include: 1) CON (basal diet); 2) T1 (basal diet+probiotic 0.1%) and 3) T2 (basal diet+probiotic+mannan rich fraction 0.2%). the data were analyzed as a randomized complete block design using of SAS University Version 4.0. The model included the effects of block (replication) and treatment. Pen served as the experimental unit. During the entire experimental period of 6 weeks, results showed that addition of complex probiotic at the level of 0.2% to diet increased ADG significantly (p<0.05). Also, digestibility of DM and N tended to increase. To sum up, results in this experiment indicated that dietary [(probiotik×acidifier)×mannan-rich-fraction]  supplementation had a positive effect on growing pigs performance and nutrient digestibility

scholarly journals 249 Evaluation of FluidQuip Dried Distillers Grains plus Solubles in Cattle Finishing Diets

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 139-139
Author(s):  
Aksel Wiseman ◽  
Brad Boyd ◽  
Levi McPhillips ◽  
Scott Tilton ◽  
Andrea K Watson ◽  
...  
Keyword(s):  
Feed Efficiency ◽  
Block Design ◽  
Linear Increase ◽  
Distillers Grains ◽  
Experimental Unit ◽  
Finishing Diets ◽  
Dried Distillers Grains ◽  
Fiber Separation ◽  
Randomized Block Design ◽  
Feeding Value

Abstract A 112 d finishing study was conducted to evaluate the effects of feeding dried distillers grains plus solubles (DDGS) from the MSC-Fiber stream of the FluidQuip post-fermentation fiber separation process compared to conventional DDGS. Yearling crossbred steers (n = 240, initial BW = 463 ± 35kg) were blocked by initial BW, stratified by BW, and assigned randomly to pen (n = 30; 8 steers/pen). Treatments were arranged as a 2×2 + 1 factorial with DDGS type (MSC DDGS [MSC]; Conventional DDGS [CONV]) and inclusion level (20 or 40% diet DM) as the factors. All treatments were compared to a corn-based control (CON). DDGS replaced a 60:40 blend of high-moisture corn and dry-rolled corn. All diets contained 7.5% alfalfa hay and supplement. Data were analyzed as a randomized block design with pen as experimental unit and block as fixed effect. Increasing concentrations of CONV or MSC resulted in a linear increase in DMI (P &lt; 0.01). Steers fed 40% MSC had the greatest DMI (P &lt; 0.01) while CON steers had the least (P &lt; 0.02). Daily gains for MSC steers linearly increased (P = 0.03), while CONV steers showed a tendency for a quadratic response to ADG (P=0.08). Feed efficiency of CONV steers increased quadratically (P = 0.04) and tended to decrease linearly (P = 0.09) for steers fed MSC. Efficiency was greatest for 20% CONV but not different (P &gt; 0.16) than CON, or 20% MSC. Steers fed 40% MSC or 40% CONV were not different in feed efficiency. Inclusion of MSC DDGS resulted in linear increases in DMI and ADG over CON, but decreased feed efficiency by 1.0 and 5.3% resulting in MSC DDGS having a relative feeding value of 95.0 and 86.7% of corn at 20 and 40% DM, respectively.

scholarly journals Increasing hay inclusion in silage-based receiving diets and its effects on performance and energy utilization in newly weaned beef steers

2020 ◽  
Vol 4 (2) ◽  
pp. 848-853
Author(s):  
Dathan T Smerchek ◽  
Elizabeth M Buckhaus ◽  
Katie D Miller ◽  
Zachary K Smith
Keyword(s):  
Growth Performance ◽  
Block Design ◽  
Corn Silage ◽  
Energy Utilization ◽  
Experimental Unit ◽  
Net Energy ◽  
Beef Steers ◽  
Linear Effect ◽  
Randomized Complete Block Design ◽  
Red Angus

Abstract The influence of grass hay (GH) inclusion in replacement of corn silage in receiving diets on growth performance and dietary net energy (NE) utilization was evaluated in newly weaned beef steers (n = 162 Charolais-Red Angus cross steers; initial body weight [BW] = 278 ± 13.4 kg). Treatments were (DM basis): 1) 0% GH, 2) 10% GH, or 3) 20% GH inclusion in replacement of corn silage in receiving diets fed to newly weaned beef steers for 56 d. The study was conducted from October to December of 2019. Data were analyzed as randomized complete block design with pen serving as the experimental unit for all analyses. Increasing dietary inclusion of hay had no influence (P ≥ 0.11) on final BW, ADG, gain:feed or observed/expected dietary NEM and NEG, observed/expected dry matter intake (DMI), or observed/expected ADG. GH inclusion increased (linear effect, P = 0.01) DMI. Observed DMI for all treatments was approximately 15% to 17% less than anticipated based upon steer growth performance and tabular NE values. Evaluation of observed/expected ADG was 31% to 37% greater than expected for the steers in the present study. Particles less than 4 mm increased (linear effect, P = 0.01) and greater than 4 mm decreased (linear effect, P = 0.01) as GH replaced corn silage in the receiving diet. As the proportion of particles greater than 4 mm increased, cumulative ADG was decreased. These data indicate that GH should be considered in corn silage-based receiving diets to improve DMI. In high-risk calves, improved DMI could result in a lesser incidence of morbidity, although no morbidity was observed in any steers from the present study.

scholarly journals 450 Late-Breaking: Effects of bedding on feedlot receiving phase growth performance in beef steers during the winter in eastern South Dakota

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 141-142
Author(s):  
Dathan Smerchek ◽  
Zachary K Smith
Keyword(s):  
Metabolic Rate ◽  
South Dakota ◽  
Growth Performance ◽  
Block Design ◽  
Average Daily Gain ◽  
Experimental Unit ◽  
Beef Steers ◽  
Phase Growth ◽  
Randomized Complete Block Design ◽  
Daily Gain

Abstract Two-hundred and forty English × Continental beef steers (initial BW=365 kg [SD 22.5]) were used in a randomized complete block design to evaluate the effects of bedding application during a 36 d feedlot receiving phase on growth performance and efficiency during winter. Steers were allotted to 30 pens (n = 8 steers/pen) at the Ruminant Nutrition Center in Brookings, SD and pens were assigned to 1 of 2 treatments: 1) No bedding applied (NO) or 2) 1.8 kg (as-is basis) of wheat straw bedding/steer/d (BED). Due to the logistics of acquiring, weighing, and timely feeding of steers, the first 9 pen replicates began on test 14 d prior (begin: January 15, 2019 and end: February 20, 2019) to the last 6 pen replicates (begin: January 29, 2019 and end: March 6, 2019). Pen was the experimental unit; an α of 0.05 determined significance. Daily ambient temperature (n = 50) averaged -14.7°C [SD 5.63] and wind-chill (n = 50) averaged -17.7°C [SD 6.40]. Initial BW (365 vs. 365 ± 0.5 kg) and dry matter intake (8.19 vs. 8.22 ± 0.047 kg) did not differ (P ≥ 0.57) between NO and BED. End BW was greater for BED (P = 0.01; 419 vs. 402 ± 1.09 kg) compared to NO. Steers from BED had increased average daily gain (P = 0.01) by 48.0% and gain:feed (P = 0.01) by 49.2% over NO. Using tabular ingredient energy values and observed steer performance shrunk 4%, relative adjustments to metabolic rate were calculated. Estimated metabolic rate was elevated (P = 0.01) for NO (0.146 vs. 0.104 ± 0.0032 Mcal/BW0.75, kg). Bedding improves feedlot receiving phase growth and efficiency in eastern South Dakota during the winter. Under the environmental conditions of this experiment, steers have a 40.4% increased metabolic rate when bedding is not used and steers in bedded pens had a 35.1% increase in metabolic rate compared to (0.077 Mcal/BW0.75, kg).

scholarly journals 230 Effects of encapsulated calcium-ammonium nitrate on performance and methane production of beef steers grazing mixed winter forage

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 166-167
Author(s):  
Andrea M Osorio Doblado ◽  
Sebastian E Mejia-Turcios ◽  
Miranda K Stotz ◽  
David Vargas ◽  
Rafael Canonenco de Araujo ◽  
...  
Keyword(s):  
Ammonium Nitrate ◽  
Methane Production ◽  
Repeated Measures ◽  
Block Design ◽  
Random Effect ◽  
Experimental Unit ◽  
Beef Steers ◽  
Adaptation Period ◽  
Calcium Ammonium Nitrate ◽  
Supplement Intake

Abstract The effects of encapsulated calcium-ammonium nitrate (eCAN) on in vivo methane production and performance were evaluated. A generalized randomized block design was used with beef steers grazing a mixed winter forage (Triticum aestivum, Triticosecale rimpaui, and Secale cereale) for 49 d. Thirty-six Angus-crossbred steers (332 ± 53 kg) were blocked by BW and randomly assigned to 1 of 3 treatments: 1) winter pasture + corn (WC), 2) WC + 328 mg/kg of BW eCAN (WCN) and 3) WC + 124 mg/kg of BW UREA (WCU), all supplemented with corn (0.3% BW treatments). Treatments WCN and WCU were isonitrogenous. A 14d adaptation period was used to adapt cattle to treatments. Methane emissions were measured using the sulfur hexafluoride tracer technique. Blood samples and BW were taken at d 0, 24, 35, and 49. Supplemental corn and NPN orts were collected daily. Data were analyzed using the MIXED procedure of SAS with the fixed effect of treatment and random effect of pasture (block); BUN, supplement intake and CP intake were analyzed with repeated measures. Steer was considered the experimental unit. Methane production was not different (P &gt; 0.05) considering g/d, g/kg of BW, g/kg of MBW, or g/kg of ADG. Treatments did not affect ADG (P = 0.941). Supplement intake was affected by treatment (P &lt; 0.001), with WC (0.979 kg) being greater compared to WCU (0.887 kg) and WCN (0.706 kg). Total CP intake increased (P &lt; 0.001) with WCU (0.155 kg) and WCN (0.116 kg), compared to WC (0.074 kg), which did not have a non-protein source. Blood urea nitrogen was affected by day, with d 24 (18.598 mg/dL) being greater compared to d 0 (8.215 mg/dL), 35 (10.549 mg/dL) and 49 (14.5744 mg/dL). The eCAN did not effectively replace urea as a NPN source to mitigate enteric methane.

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