scholarly journals 284 Gene polymorphism of 5, 10-methylene tetrahydrofolate reductase is related to boar semen quality

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 117-117
Author(s):  
wen lai ◽  
chao Wang ◽  
Jian Peng
Keyword(s):  
Semen Quality ◽  
Mthfr Gene ◽  
Missense Mutations ◽  
Double Mutation ◽  
Polymorphic Loci ◽  
Synonymous Mutations ◽  
Methylene Tetrahydrofolate Reductase ◽  
Boar Semen ◽  
Methylene Tetrahydrofolate

Abstract 5, 10-methylene tetrahydrofolate reductase (MTHFR) is an important enzyme in folate and homocysteine metabolism, and plays an important role in regulating folate levels, DNA synthesis and methylation in cells. Defects in the MTHFR gene can lead to spermatogenesis disorders and male sterility. Polymorphic loci of MTHFR gene and semen quality of 1,490 boars were examined to explore their relationship. Results showed there were 13 polymorphic loci on MTHFR gene exon in boars with different semen quality, among which loci 1 and 2 were missense mutations. For Duroc boars, although there were synonymous mutations at loci 3–5 and 11, the mutation had no effect on semen quality (P > 0.05). In addition to loci 13, loci 1–12 of the MTHFR gene had mutations, but it had no effect on semen quality in Yorkshire boars (P > 0.05). Interestingly, for Landrace boars, double mutation of MTHFR gene at 1–2, 6–10, and 12 loci (CC → TT) led to decreased sperm motility and increased abnormal sperm rate (P < 0.05). Further analysis showed seminal plasma MDA and hydrogen peroxide levels were increased in Landrace boars with the mutation of MTHFR gene at loci 1–2, 6–10 and 12 (P < 0.05). In conclusion, double mutation of MTHFR gene at 1–2, 6–10, and 12 loci decreased semen quality and increased oxidative stress in sperm, and these mutant loci may be potential biomarkers for predicting the semen quality of Landrace boars.

scholarly journals 382 Gene polymorphism of 5, 10-methylene tetrahydrofolate reductase is related to boar semen quality

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 143-144
Author(s):  
wen lai ◽  
chao Wang ◽  
Jian Peng
Keyword(s):  
Semen Quality ◽  
Mthfr Gene ◽  
Missense Mutations ◽  
Double Mutation ◽  
Polymorphic Loci ◽  
Synonymous Mutations ◽  
Methylene Tetrahydrofolate Reductase ◽  
Boar Semen ◽  
Methylene Tetrahydrofolate

Abstract 5, 10-methylene tetrahydrofolate reductase (MTHFR) is an important enzyme in folate and homocysteine metabolism, and plays an important role in regulating folate levels, DNA synthesis and methylation in cells. Defects in the MTHFR gene can lead to spermatogenesis disorders and male sterility. Polymorphic loci of MTHFR gene and semen quality of 1,490 boars were examined to explore their relationship. Results showed there were 13 polymorphic loci on MTHFR gene exon in boars with different semen quality, among which loci 1 and 2 were missense mutations. For Duroc boars, although there were synonymous mutations at loci 3–5 and 11, the mutation had no effect on semen quality (P > 0.05). In addition to loci 13, loci 1–12 of the MTHFR gene had mutations, but it had no effect on semen quality in Yorkshire boars (P > 0.05). Interestingly, for Landrace boars, double mutation of MTHFR gene at 1–2, 6–10, and 12 loci (CC → TT) led to decreased sperm motility and increased abnormal sperm rate (P < 0.05). Further analysis showed seminal plasma MDA and hydrogen peroxide levels were increased in Landrace boars with the mutation of MTHFR gene at loci 1–2, 6–10 and 12 (P < 0.05). In conclusion, double mutation of MTHFR gene at 1–2, 6–10, and 12 loci decreased semen quality and increased oxidative stress in sperm, and these mutant loci may be potential biomarkers for predicting the semen quality of Landrace boars.

Value of semen parameters, with special reference to TNF-α, in predicting the quality of boar semen after short-term storage

2013 ◽  
Vol 61 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Janko Mrkun ◽  
Marjan Kosec ◽  
Petra Zrimšek
Keyword(s):  
Semen Quality ◽  
Semen Parameters ◽  
Short Term ◽  
Progressive Motility ◽  
Tnf Α ◽  
Semen Storage ◽  
Boar Semen ◽  
Α Level ◽  
Term Storage

The aim of this study was to address the question whether changes in boar semen quality after short-term storage could be predicted on the basis of standard semen parameters and TNF-α level determined on the day of semen collection under commercial conditions. Progressive motility showed the highest positive correlation with morphology on day 0 of collection, and progressive motility on day 3 (P < 0.05) showed a negative correlation with acrosome abnormalities (P < 0.05). According to the area under receiver operating characteristics (ROC) curves (AUCs), progressive motility could also be used in predicting semen quality after 3 days of storage (AUC > 0.5; P < 0.05). TNF-α in seminal plasma is the only parameter measured on day 0 to show a significant correlation with the percentage of viable spermatozoa after 3 days of semen storage (r = 0.495, P < 0.05). ROC analysis shows that TNF-α level is helpful in discriminating viability outcome after semen storage (AUC = 0.94, P < 0.001). We can predict with 92.35% certainty that fresh semen samples with more than 150 pg/ml of TNF-α in the seminal plasma will retain more than 85% of viable spermatozoa after 3 days of storage. Thus, TNF-α can contribute to predicting the quality of short-term stored semen.

scholarly journals A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

2016 ◽  
Vol 85 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Marina Anastasia Karageorgiou ◽  
Georgios Tsousis ◽  
Constantin M. Boscos ◽  
Eleni D. Tzika ◽  
Panagiotis D. Tassis ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Medium Term ◽  
Semen Collection ◽  
Chromatin Integrity ◽  
Boar Semen ◽  
Short Time

The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16–18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research.

scholarly journals Efficacy of potassium permanganate and turmeric as antimicrobial agents on the bacterial load and quality of boar semen during preservation at 15 °C

2020 ◽  
Vol 90 (5) ◽  
pp. 443-451
Author(s):  
Nang Kanna Manpoong ◽  
Kutubuddin Ahmed ◽  
Dilip Kumar Bhattacharya ◽  
Dipak Kumar Sarma ◽  
Nekibuddin Ahmed ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Semen Quality ◽  
Sperm Quality ◽  
Bacterial Load ◽  
Conception Rate ◽  
E Coli ◽  
Bacillus Spp ◽  
Boar Semen ◽  
Conventional Antibiotics

The present study was conducted to evaluate the efficacy of different natural antimicrobials agents (KMnO4 and Turmeric) in comparison with conventional antibiotics against the bacterial load and in relation to the quality of boar semen in Modena extender for up to 120 hours of preservation at 15 °C. A total of 56 ejaculates, 14 from each of four Hampshire crossbred boars maintained within the ICAR-AICRP on Pigs, in Guwahati, Assam, India, were utilized in the study. Thirty-two ejaculates, 8 from each of four boars were used to study the effect of antimicrobial agents on semen quality during preservation at 15 °C in Modena extender. A total of 9 different bacterial types were identified from 46 bacterial isolates, obtained from 24 fresh semen samples viz. Staphylococcus aureus (24%), E. coli (22%), Bacillus spp. (13%), Citrobacter spp. (9%), Pseudomonas spp. (9%), Staphylococcus epidermidis (9%), Klebsiella spp. (6%), Streptococcus spp. (6%) and Proteus spp. (2%). The overall sensitivity of the recovered isolates to Gentamicin, Ampicillin, Enrofloxacin, Cloxacillin, Streptomycin, Penicillin, Amoxycilln, Ofloxacin and Tetracyclin were 89, 39, 37, 48, 74, 52, 56, 76 and 63% respectively. The mean sperm motility, intact acrosome, HOST-reacted spermatozoa and bacterial load differed significantly (P˂0.01) between antimicrobial agents (Gentamicin, KMnO4 and Turmeric) and preservation periods (0, 48, 72, 96 and 120 hours). Sperm quality based on Gentamicin was found to be best, followed by Turmeric and KMnO4 during preservation at 15 °C. The conception rate for the semen preserved for 0, 24, 48, 72, 96 and 120 hours of preservation was 83.33, 80.00, 75.00, 66.66, 66.66 and 50.00% respectively. In the present study, the preserved semen with ascending bacterial load containing Gentamicin did not affect the conception rate.

scholarly journals Evaluate the activity of cryopreserved boar ejaculated spermatozoa under different thawing protocols

2020 ◽  
Vol 27 ◽  
pp. 287-292
Author(s):  
O. V. Shcherbak ◽  
S. I. Kovtun ◽  
O. I. Metlitska ◽  
P. A. Trotskyi ◽  
I. M. Lyuta ◽  
...  
Keyword(s):  
Semen Quality ◽  
Genetic Material ◽  
In Vitro Cultivation ◽  
Individual Level ◽  
Cryopreserved Sperm ◽  
Boar Semen ◽  
The Individual ◽  
Breeding And Genetics

Aim. To evaluate the activity of cryopreserved boar ejaculated spermatozoa under different thawing protocols to optimize biotechnological approaches in pig reproduction based on the genetic material of the Institute of Animal Breeding and Genetics nd. a. M.V. Zubets of National Academy of Agrarian Science of Ukraine. Methods. Cryopreserved ejaculated boars' sperm was thawed in three different ways. Biotechnological, cryobiological and morphological methods were used to assess the viability of the sperm. Results. We noted the individual feature of semen quality of the studied boars, which affects its suitability for cryopreservation and quality indicators after thawing. It was found that in liquefied boar sperm (sperm activity on average is 86.7%) after its centrifugation and three-hour equilibration at + 4 °C sperm activity decreased by an average of 25.0%. In order to improve the quality of cryopreserved sperm, it was thawed under different conditions. The highest activity of spermatozoa in thawed sperm of boar No. 225 of Myrhorod pig was at the level of 25.0% at the thawing temperature + 70 °C, and the thermal resistance and heat resistance were 70.0% and 60.0%, respectively. Conclusions. During this study was noted that there are peculiarities of boar semen at breed and individual level, which influence its ability to tolerate cryopreservation. Keywords: sperm, in vitro cultivation, cryobank, cryopreservation, boars.

The effect of prostaglandin f2α on the production and quality of boar semen

1991 ◽  
Vol 1991 ◽  
pp. 141-141
Author(s):  
Ronald Melendez ◽  
O MacPherson ◽  
J A Roden ◽  
S A Edwards ◽  
J S H Hutchinson ◽  
...  
Keyword(s):  
Litter Size ◽  
Semen Quality ◽  
Prostaglandin F2α ◽  
Progeny Testing ◽  
Human Semen ◽  
Sperm Velocity ◽  
Commercial Practice ◽  
Boar Semen ◽  
Effective Use

The breeding boar is the single most important animal in the pig herd because he can sire very many piglets in a year, even moreso when used for AI. When a very superior boar is isolated following performance or progeny testing, good management and AI technology can be combined to exploit his potential. One possible way of making even more effective use of such a sire is through treatment of both the boar and his semen with analogues of prostaglandin F2α (PGF2α ). There have been reports from commercial practice of treatment of boars with PGF2a having desirable influences on libido, an increase in ejaculate volume and subsequent litter size in sows they serve. There have also been claims that when human semen is treated directly with an analogue of PGF2α sperm velocity increases. The purpose of this study was to further investigate the effects of PGF2 on semen production and semen quality of boars.

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