PSXIII-1 Age-related changes in the ratio of different spermatogonia types in the seminiferous tubules of quail testes
Abstract Spermatogonia are early-undifferentiated germ cells, giving rise to mature male generative cells — the spermatozoa. There are two types of spermatogonia – A and B. Of greatest interest is the use of type A spermatogonia, which are the stem cells of the testes. To select the appropriate age for collecting spermatogonia А from quails it is necessary to know the specific features of spermatogenesis. Development dynamics of various spermatogonia types in the quail testicular tubules was studied. Histological studies of the quails testicular tubules at the age of 1, 2, 3, 4, 5 and 6 weeks (n = 30) were carried out. Samples of testis tissue were fixed in Bouin’s fixative. Histological sections were stained with hematoxylin-eosin. Identification of different spermatogonia types was carried out according to their morphology. Type A spermatogonia were additionally identified by immunohistochemistry using SSEA-1 antibodies. The proportion of spermatogonia in the total number of spermatogenic cells in the seminiferous tubules of quails changed with age. The maximum value was reached at the age of 3 weeks and it was 76±6%. On reaching maturity (6 weeks), this indicator decreased to 12 ± 1 %. In the early period of ontogenesis (1–2 weeks), spermatogonia cells were represented mainly by type A spermatogonia. The proportion of these cells from the total number of spermatogonia reached 80 ± 3 %. With increasing age, this indicator decreased, reaching minimum values for achieving maturity (6 weeks) - 16 ± 1 %. The percentage of type B spermatogonia in the seminiferous tubule of quails on the contrary increased with age — from 5 ± 1% at 1 week old to 70 ± 2% at maturity. Thus, the age no later than 2 weeks is the most optimal for the isolation type A spermatogonia of quails. Supported by RFBR (18-29-07079).