Evaluate the Effect of Zinc Oxide and Silver Nanoparticles on Biofilm and icaA Gene Expression in Methicillin-Resistant Staphylococcus aureus Isolated From Burn Wound Infection

2020 ◽  
Vol 41 (6) ◽  
pp. 1253-1259 ◽  
Author(s):  
Ali Shakerimoghaddam ◽  
Delaramsadat Razavi ◽  
Farzaneh Rahvar ◽  
Maria Khurshid ◽  
Shokoufeh Mogharabi Ostadkelayeh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Zinc Oxide ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Formation Rate ◽  
Ag Nps ◽  
Methicillin Resistant ◽  
Icaa Gene

Abstract Methicillin-resistant Staphylococcus aureus is the cause of nosocomial and community-acquired infections. This study aimed to evaluate the effect of zinc oxide and silver nanoparticles (ZnO-Ag NPs) on biofilms formation and icaA gene expression in methicillin-resistant S. aureus (MRSA). In this study, three standard strains (ATCC 43300, 25923, and 29913) and a clinical isolate are included. The minimum inhibitory concentration (MIC) of nanoparticles was determined by microdilution broth method. The antibacterial effects of ZnO-Ag NPs either alone or in combination with each other were compared with vancomycin (as the control group). The effect of MIC and sub-MIC concentrations of ZnO-Ag NPs on biofilm formation was determined by the microtiter plate method. The expression level of the icaA gene was assessed by real-time PCR LightCycler® 96 software (Version 1.1.0.1320, Roche, Germany). technique. All experiments were repeated three times. Data were analyzed using SPSS software through ANOVA and t-test. The P-value of less than .05 was considered as statistically significant. The average MICs of ZnO, Ag, and ZnO-Ag NPs compounds were 393.2, 179.8, and 60.8 μg/ml, respectively. The compound of ZnO-Ag NPs had a synergistic effect against all isolates. ZnO-Ag NPs decreased the biofilm formation rate at MIC and sub-MIC concentrations (P < .001). Sub-MIC ZnO-Ag NPs concentration significantly reduced the icaA gene expression in S. aureus strains (P < .03). The sub-MIC concentration of ZnO-Ag NPs reduced biofilm formation rate and icaA gene expression in Staphylococcus aureus strains compared with vancomycin. It can be used to cover medical devices after examining more clinical isolates to prevent bacterial colonization.

scholarly journals Graphene Decorated Zinc Oxide and Curcumin to Disinfect the Methicillin-Resistant Staphylococcus aureus

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 1004
Author(s):  
Mohammad Oves ◽  
Mohd. Ahmar Rauf ◽  
Mohammad Omaish Ansari ◽  
Aftab Aslam Parwaz Khan ◽  
Huda A Qari ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Zinc Oxide ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Wall Structure ◽  
Resistant Bacteria ◽  
Mice Model ◽  
Zno Nps ◽  
Methicillin Resistant ◽  
Wide Range

Sometimes, life-threatening infections are initiated by the biofilm formation facilitated at the infection site by the drug-resistant bacteria Staphylococcus aureus. The aggregation of the same type of bacteria leads to biofilm formation on the delicate tissue, dental plaque, and skin. In the present investigation, a Graphene (Gr)-based nano-formulation containing Curcumin (C.C.M.) and Zinc oxide nanoparticles (ZnO-NPs) showed a wide range of anti-microbial activity against Methicillin-resistant Staphylococcus aureus (MRSA) biofilm and demonstrated the anti-microbial mechanism of action. The anti-microbial effect of GrZnO nanocomposites, i.e., GrZnO-NCs, suggests that the integrated graphene-based nanocomposites effectively suppressed both sensitive as well as MRSA ATCC 43300 and BAA-1708 isolates. The S. aureus inhibitory effect of GrZnO-NCs improved >5-fold when combined with C.C.M., and demonstrated a M.I.C. of 31.25 µg/mL contrasting with the GrZnO-NCs or C.C.M. alone having M.I.C. value of 125 µg/mL each. The combination treatment of GrZnO-NCs or C.C.M. inhibited the M.R.S.A. topical dermatitis infection in a mice model with a significant decrease in the CFU count to ~64%. Interestingly, the combination of C.C.M. and GrZnO-NCs damaged the bacterial cell wall structure, resulting in cytoplasm spillage, thereby diminishing their metabolism. Thus, owing to the ease of synthesis and highly efficient anti-microbial properties, the present graphene-based curcumin nano-formulations can cater to a new treatment methodology against M.R.S.A.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND DETECTION OF VIRULENCE FACTORS - NUCLEASE, PHOSPHATASE, AND BIO FILM PRODUCTION

2018 ◽  
Vol 11 (5) ◽  
pp. 224 ◽  
Author(s):  
Manipriya B ◽  
Tasneem Banu ◽  
Prem Kumar L ◽  
Kalyani M
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Virulence Factors ◽  
Bactericidal Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Nano Particles ◽  
Clinical Isolates ◽  
Ag Nps ◽  
Methicillin Resistant

 Objective: To determine the virulence factors-biofilm, nuclease and phosphatase production in Staphylococcus aureus isolates. To determine the effect of silver nano particles and antibiotics on MRSA by MIC determination and kirby baeur method respectively and finally to compare antibacterial activity of silver nano particles and antibiotics.Methods: In the present study, we explore the antibacterial activity of silver nanoparticles (Ag-NPs) dispersion (10 nm) against reference strain and clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). We evaluated the antibacterial activity of Ag-NPs against MRSA isolated from patients in Saveetha Medical College and Hospital, a tertiary care centre in Chennai, Tamil Nadu. The bactericidal activity of different concentrations of Ag-NPs (200, 100, 50, 25, 12.5, 6.25, 3.125, and 1.5625 μg/ml) was tested by determining MIC using microbroth dilution and MBC by agar dilution methods.. In addition, the virulence factors phosphatase, nuclease, and biofilm production were tested.Result: The values of minimal inhibitory concentration and minimal bactericidal concentration of Ag-NPs against all clinical isolates of MRSA and a single of S. aureus were found in the range of 12.5–50 μg/ml and 12.5–25 μg/ml, respectively, indicating very good bactericidal activity. Ag-NPs with the highest concentration showed almost no growth for up to 16 h representing a bactericidal effect at this concentration. Effect was proportional to dose since 50.0 μg/ml was the most effective dose since the bacterial population did not recover and 12.5 μg/ml was the least effective. All the MRSA isolates were positive for the virulence factors.Conclusion: The study result suggests that Ag-NPs could be used as an effective alternative antibacterial agent.

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