Effect of Zolpidem Tartrate on the Developmental Rate of Forensically Important Flies Chrysomya megacephala (Diptera: Calliphoridae) and Chrysomya saffranea

2021 ◽  
Author(s):  
Laila A Al-Shuraym ◽  
Fahd A Al-Mekhlafi ◽  
Fahd Mohammed Abd Al Galil ◽  
Sadeq K Alhag ◽  
Lamya Ahmed Al-Keridis ◽  
...  
Keyword(s):  
Developmental Rate ◽  
Morphological Parameters ◽  
Time Of Death ◽  
Forensic Investigation ◽  
Negatively Associated ◽  
Chrysomya Megacephala ◽  
Sleeping Disorders ◽  
Cellular Physiology ◽  
Life Cycle Stages ◽  
Evidence Evaluation

Abstract Entomotoxicology is an emerging science in the forensic investigation of death; it now influences judicial activities. Briefly, carrion flies ingest various chemicals as they feed on a cadaver. In entomotoxicology, such toxicological compounds are qualitatively and quantitatively analyzed in insects recovered from carrion. Some of these chemicals can disrupt flies’ cellular physiology, leading to acceleration or delay in their developmental rate. In this study, the effect of zolpidem-contaminated tissue on the dipteran flies Chrysomya megacephala (Fabricius 1794) and Chrysomya saffranea (Bigot 1877) was assessed. Zolpidem tartrate is a sedative-hypnotic compound commonly used to treat anxiety and sleeping disorders, but it is associated with death from suicide. The effects were tested by providing the flies with liver tissue treated with various concentrations of zolpidem tartrate. The morphological parameters and development rate for all life cycle stages of treated insects were determined and used to estimate postmortem intervals. Results showed that the morphological parameters, i.e., weight, width, and length and rate of development of C. megacephala and C. saffranea was negatively associated with zolpidem tartrate concentration of C. saffranea, were negatively associated with the concentration of zolpidem tartrate, indicating that arthropod evidence evaluation can help establish the cause and time of death.

scholarly journals Developmental rate of Chrysomya megacephala (Fabricius) (Insecta, Diptera, Calliphoridae), a species of forensic importance, under alternating temperature

2019 ◽  
Author(s):  
Gabriela da Silva Zampim ◽  
Patricia Jacqueline Thyssen ◽  
Ângela L. Daltin ◽  
Lucas T. Oliveira ◽  
Gustavo A. Faria
Keyword(s):  
Developmental Rate ◽  
Time Of Death ◽  
Extrinsic Factors ◽  
Rate Of Development ◽  
Chrysomya Megacephala ◽  
Development Models ◽  
Field Temperature ◽  
Alternating Temperature

Chrysomya megacephala (Fabricius) (Insecta, Diptera, Calliphoridae), originating in Asia and currently found in the African and American continents, may be associated with decomposing bodies due to its necrophagous habit. For this reason, it gains importance in the forensic field. Temperature is one of the most important extrinsic factors in the life of insects because it directly affects their metabolism. In the literature only data on the development of forensically important insects under controlled temperature are available. Thus, this study aimed to evaluate the rate of development of C. megacephala under two sets of alternating temperatures (22.5-32.5°C and 20-35°C). The results obtained here may be useful for obtaining more accurate development models to estimate the time of death in criminal events.

scholarly journals Corrigendum to: Effect of Zolpidem Tartrate on the Developmental Rate of Forensically Important Flies Chrysomya megacephala (Diptera: Calliphoridae) and Chrysomya saffranea

2021 ◽  
Author(s):  
Laila A Al-Shuraym ◽  
Fahd A Al-Mekhlafi ◽  
Fahd Mohammed Abd Al Galil ◽  
Sadeq K Alhag ◽  
Lamya Ahmed Al-Keridis ◽  
...  
Keyword(s):  
Developmental Rate ◽  
Chrysomya Megacephala

scholarly journals Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Yulia M. Serebrennikova ◽  
Debra E. Huffman ◽  
Luis H. Garcia-Rubio
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Parameters ◽  
Host Interactions ◽  
Disease States ◽  
Life Cycle Stages ◽  
Infected Rbcs ◽  
Rbc Indices

Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, withR2>0.85for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasitePlasmodium falciparumat different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites’ development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

scholarly journals Developmental rate of Synthesiomyia nudiseta (van der Wulp, 1883) (Insecta, Diptera, Muscidae), a species of forensic importance, under alternating temperature

2018 ◽  
Author(s):  
Aline Marrara do Prado ◽  
Patricia Jacqueline Thyssen ◽  
Barbara Maria Cerqueira Maia ◽  
Bryan Gomes Ribeiro da Silva ◽  
Rafael Vergílio
Keyword(s):  
Development Time ◽  
Developmental Rate ◽  
Neotropical Region ◽  
Time Of Death ◽  
Extrinsic Factors ◽  
Rate Of Development ◽  
Development Models ◽  
Field Temperature ◽  
Alternating Temperature

Synthesiomyia nudiseta (van der Wulp, 1883) (Insecta, Diptera, Muscidae) is widely distributed in the Neotropical region. It may be associated with decomposing bodies due to its necrophagous habit, for this reason it gains importance in the forensic field. Temperature is one of the most important extrinsic factors in the life of insects because it directly affects their metabolism. In the literature only data on the development time of forensically important insects under controlled temperature are available. Thus, this study aimed to evaluate the rate of development of S. nudiseta under alternating temperatures. The results obtained here may be useful for obtaining more accurate development models to estimate the time of death in criminal events.

scholarly journals Time of Death Revealed by Hydrocarbons of Empty Puparia of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae): A Field Experiment

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73043 ◽  
Author(s):  
Guang-Hui Zhu ◽  
Xiao-Jun Yu ◽  
Liang-Xing Xie ◽  
Hao Luo ◽  
Dian Wang ◽  
...  
Keyword(s):  
Field Experiment ◽  
Time Of Death ◽  
Chrysomya Megacephala

Morphology and developmental rate of blowflies Chrysomya megacephala and Chrysomya rufifacies in Thailand: application in forensic entomology

2008 ◽  
Vol 102 (6) ◽  
pp. 1207-1216 ◽  
Author(s):  
Kom Sukontason ◽  
Somsak Piangjai ◽  
Sirisuda Siriwattanarungsee ◽  
Kabkaew L. Sukontason
Keyword(s):  
Forensic Entomology ◽  
Developmental Rate ◽  
Chrysomya Rufifacies ◽  
Chrysomya Megacephala

Effect of diets on the developmental rate of calliphorid fly of forensic importance Chrysomya megacephala (Fabricius, 1794)

2021 ◽  
Author(s):  
Rania Ali El Hadi Mohamed ◽  
Fahd Mohammed Abd Al Galil ◽  
Lamya Ahmed Al-Keridis ◽  
Laila A. Al-Shuraym ◽  
Fahd A. AL-mekhlafi ◽  
...  
Keyword(s):  
Developmental Rate ◽  
Chrysomya Megacephala

scholarly journals Associations Between the Morphological Parameters of Proximal Tibiofibular Joint (PTFJ) and Changes in Tibiofemoral Joint Structures in Patients with Knee Osteoarthritis

2021 ◽  
Author(s):  
Jun Chang ◽  
Tianyu Chen ◽  
Yizhu Yan ◽  
Zhaohua Zhu ◽  
Weiyu Han ◽  
...  
Keyword(s):  
Knee Osteoarthritis ◽  
Regression Models ◽  
Structural Changes ◽  
Cartilage Volume ◽  
Cartilage Defects ◽  
Proximal Tibiofibular Joint ◽  
Morphological Parameters ◽  
Load Bearing ◽  
Negatively Associated ◽  
Tibiofibular Joint

Abstract BackgroundTo describe the longitudinal associations between the morphological parameters of proximal tibiofibular joint (PTFJ) and joint structural changes in tibiofemoral compartments in patients with knee osteoarthritis (OA).MethodsThe participants were selected from the Vitamin D Effects on Osteoarthritis (VIDEO) study. PTFJ morphological parameters were measured on coronal and sagittal MRI. The contacting area (S) of PTFJ, and its projection areas onto the horizontal (load-bearing area, Sτ), sagittal (lateral stress-bolstering area, Sφ) and coronal plane (posterior stress-bolstering area, Sυ) were assessed. Knee structural abnormalities, including cartilage defects, bone marrow lesions (BMLs) and cartilage volume, were evaluated at baseline and after 2 years. Log binominal regression models and linear regression models were used to assess the associations between PTFJ morphological parameters and osteoarthritic structural changes.ResultsIn the longitudinal analyses, the S (RR: 1.45) and Sτ (RR: 1.55) of PTFJ were significantly and positively associated with an increase in medial tibial (MT) cartilage defects. The Sτ (β: -0.07), Sυ (β: -0.07), and S (β: -0.06) of PTFJ were significantly and negatively associated with changes in MT cartilage volume. The Sτ (RR: 1.55) of PTFJ was positively associated with an increase in MT BMLs, and Sφ (RR: 0.35) was negatively associated with an increase in medial femoral BMLs. ConclusionsThis longitudinal study suggests that higher load-bearing area of PTFJ could be a risk factor for structural changes in medial tibiofemoral (MTF) compartment in knee OA. This may provide a theoretical support for proximal fibular osteotomy in the treatment of MTF OA.Trial registration: Clinicaltrials.gov Identifier: NCT01176344; Anzctr.org.au Identifier: ACTRN12610000495022Date of registration: 7 May 2010

scholarly journals Morphology and Developmental Rate of the Blow Fly,Hemipyrellia ligurriens(Diptera: Calliphoridae): Forensic Entomology Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Nophawan Bunchu ◽  
Chinnapat Thaipakdee ◽  
Apichat Vitta ◽  
Sangob Sanit ◽  
Kom Sukontason ◽  
...  
Keyword(s):  
Light Microscope ◽  
Forensic Entomology ◽  
Development Time ◽  
Developmental Rate ◽  
Developmental Time ◽  
Morphological Features ◽  
Forensic Investigation ◽  
Blow Fly ◽  
Ambient Conditions ◽  
Complete Metamorphosis

Hemipyrellia ligurriens(Diptera: Calliphoridae) is a forensically important blow fly species presented in many countries. In this study, we determined the morphology of all stages and the developmental rate ofH. ligurriensreared under natural ambient conditions in Phitsanulok province, northern Thailand. Morphological features of all stages based on observing under a light microscope were described and demonstrated in order to use for identification purpose. Moreover, development time in each stage was given. The developmental time ofH. ligurriensto complete metamorphosis; from egg, larva, pupa to adult, took 270.71 h for 1 cycle of development. The results from this study may be useful not only for application in forensic investigation, but also for study in its biology in the future.

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