Expressions of OsHKT1, OsHKT2, and OsVHA are differentially regulated under NaCl stress in salt-sensitive and salt-tolerant rice (Oryza sativa L.) cultivars

Salt-tolerant mutants are valuable resources for basic and applied research on plant salt tolerance. Here, we report the isolation and characterisation of a salt-tolerant rice (Oryza sativa L.) mutant. This mutant was identified from an ethyl methanesulfonate-induced Nipponbare mutant library, designated as rice salt tolerant 1 (rst1). The rst1 mutant was tolerant to salt stress and showed significantly higher shoot biomass and chlorophyll content, but lower lipid peroxidation and electrolyte leakage under NaCl stress. The improved salt tolerance of this mutant may be due mainly to its enhanced ability to restrict Na+ accumulation in shoots under salt stress conditions. Genetic analysis indicated that the salt tolerance of the rst1 mutant was controlled by a single recessive gene. Quantitative trait locus (QTL) mapping for salt tolerance was performed using an F2 population of rst1 × Peiai 64. Two QTLs were detected, in which the locus on chromosome 6 was determined to be the candidate locus of the rst1 gene. The rst1 locus was subsequently shown to reside within a 270.4-kb region defined by the markers IM29432 and IM29702. This result will be useful for map-based cloning of the rst1 gene and for marker-assisted breeding for salt tolerance in rice.

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Differential responses of antioxidative defense system to prolonged salinity stress in salt-tolerant and salt-sensitive Indica rice (Oryza sativa L.) seedlings

PROTOPLASMA ◽

10.1007/s00709-011-0365-3 ◽

2011 ◽

Vol 250 (1) ◽

pp. 3-19 ◽

Cited By ~ 104

Author(s):

Pallavi Mishra ◽

Kumari Bhoomika ◽

R. S. Dubey

Keyword(s):

Oryza Sativa ◽

Salinity Stress ◽

Oryza Sativa L ◽

Indica Rice ◽

Antioxidative Defense ◽

Defense System ◽

Salt Tolerant ◽

Antioxidative Defense System ◽

Differential Responses

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Differential expression of salt-responsive genes to salinity stress in salt-tolerant and salt-sensitive rice (Oryza sativa L.) at seedling stage

PROTOPLASMA ◽

10.1007/s00709-018-1257-6 ◽

2018 ◽

Vol 255 (6) ◽

pp. 1667-1681 ◽

Cited By ~ 13

Author(s):

Vijayata Singh ◽

Ajit Pal Singh ◽

Jyoti Bhadoria ◽

Jitender Giri ◽

Jogendra Singh ◽

...

Keyword(s):

Oryza Sativa ◽

Differential Expression ◽

Salinity Stress ◽

Oryza Sativa L ◽

Seedling Stage ◽

Salt Tolerant

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Characterization of rice (Oryza sativa L.) plants regenerated from salt-tolerant cell lines

Plant Science ◽

10.1016/0168-9452(95)04274-1 ◽

1996 ◽

Vol 113 (1) ◽

pp. 105-111 ◽

Cited By ~ 29

Author(s):

Ilga Winicov

Keyword(s):

Oryza Sativa ◽

Cell Lines ◽

Oryza Sativa L ◽

Salt Tolerant ◽

Tolerant Cell

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Salinity Results in Polyamine Accumulation in Early Rice (Oryza sativa L.). Seedlings

Functional Plant Biology ◽

10.1071/pp9880777 ◽

1988 ◽

Vol 15 (6) ◽

pp. 777 ◽

Cited By ~ 28

Author(s):

R Basu ◽

N Maitra ◽

B Ghosh

Keyword(s):

Oryza Sativa ◽

Amino Acid ◽

Potent Inhibitor ◽

Oryza Sativa L ◽

Arginine Decarboxylase ◽

Nacl Stress ◽

Leucine Incorporation ◽

Polyamine Content ◽

Early Rice

Saline (NaCl) stress in germinated seedlings of rice cv. Rupsail causes an increase in polyamine content and concomitantly an enhancement of arginine decarboxylase (ADC) activity; in the case of putrescine, the increase is 100%. The responsiveness of coleoptiles and roots is different; ADC activity is maximum in roots and coleoptiles at 50 mM and 100 mM respectively. Difluoromethyl arginine, a potent inhibitor of ADC, reduces both polyamine accumulation and ADC activity. NaCl can enhance the activity of ADC by 1.6-fold in vitro. [14C]leucine incorporation into protein at 400 mM NaCl is decreased 4- and 10-fold in coleoptiles and roots, respectively. Efflux of sugar, amino acid, polyamines and total electrolytes increased gradually with the increase in concentration of salt. Influx of Na+ and Cl- and efflux of K+ in coleoptiles and roots are directly proportional to the concentration of NaCl applied.

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Characterization of Progenies from Intergeneric Hybridization Between Oryza sativa L. and Porteresia coarctata (Roxb.) Tateoka

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v27i1.35013 ◽

2017 ◽

Vol 27 (1) ◽

pp. 63-76

Author(s):

Tasmia Islam ◽

Sudip Biswas ◽

Umme Habiba Mita ◽

RH Sarker ◽

M Sazzadur Rahman ◽

...

Keyword(s):

Oryza Sativa ◽

Ssr Markers ◽

Oryza Sativa L ◽

Intergeneric Hybridization ◽

Chromosome Loss ◽

Salt Tolerant ◽

Porteresia Coarctata ◽

Maternal Parent ◽

Hybrid Plants ◽

Coastal Belt

Porteresia coarctata (Roxb.) Tateoka is an endemic halophyte growing all over the coastal belt of Bangladesh, propagating through rhizomes and setting a few ricelike grains. So exploiting the genetic potential of this wild rice as salt tolerant donor in possible wide crosses with rice (2n = 24) could be useful. We attempted intergeneric hybridization between Oryza sativa L. and P. coarctata. The survival rate of hybrid progenies in embryo culture was low but among them 2 hybrid plants were successfully matured from the intergeneric cross between the cultivated induced tetraploid of rice, Latisail (2n = 4x = 48) and P. coarctata (2n = 48). The hybrid plants could be successfully established in soil and were not like either of the parents in morphology although some of their features were similar to their maternal parent, Latisail (4x). Both of the hybrids were investigated through physiological analysis under salinity stress and molecular analyses with rice specific SSR markers. Molecular analysis of the F1 DNA with only 3 SSR markers, RM581, RM20224 and RM25271, out of 36 others tested, showed bands specific to both of the parents, while all had common bands with the maternal parent. Dendrogram analysis of the hybrids with the 36 SSR markers, show that P. coarctata forms a different clade and is clearly separated from Latisail and the hybrids. The putative hybrids however made a subgroup with Latisail. These observations could be possibly explained if chromosome loss of the paternal parent had occurred or may be it was a pleotropic effect of intergeneric hybridization. Physiological screening of the hybrid progenies at the F2 generation in seedling stage showed better result in leaf damage score (LDS) and salinity tolerance than their maternal parent Latisail (4x) at 150 mM salt stress for 10 days. F2 plants from one of the hybrid plants (H‐2) showed better performance but there was a large variation in response from each of the individual progenies. So, it is likely that some of the salt tolerant characteristics of the pollen parent might have been transferred to the recipient Latisail (4x). For introgression of better salt tolerant loci from P. coarctata, more wide hybrids will need to be produced and repeatedly crossed with P. coarctata.Plant Tissue Cult. & Biotech. 27(1): 63-76, 2017 (June)

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Identification of Up-regulated Transcripts in Salt Tolerant Rice (Oryza sativa L.) Cultivar using the cDNA-AFLP Technique

Journal of Crop Breeding ◽

10.29252/jcb.9.23.125 ◽

2017 ◽

Vol 9 (23) ◽

pp. 125-137

Author(s):

Fatemeh Zare ◽

Gholam Ali Ranjbar ◽

Ali Reza Tarang ◽

Hamid Najafi Zarrini ◽

◽

...

Keyword(s):

Oryza Sativa ◽

Oryza Sativa L ◽

Salt Tolerant ◽

Aflp Technique

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Evaluation and clustering on salt-tolerant ability in rice genotypes (Oryza sativa L. subsp. indica) using multivariate physiological indices

Physiology and Molecular Biology of Plants ◽

10.1007/s12298-018-00636-2 ◽

2019 ◽

Vol 25 (2) ◽

pp. 473-483 ◽

Cited By ~ 1

Author(s):

Wasinee Pongprayoon ◽

Rujira Tisarum ◽

Cattarin Theerawittaya ◽

Suriyan Cha-um

Keyword(s):

Oryza Sativa ◽

Oryza Sativa L ◽

Salt Tolerant ◽

Physiological Indices ◽

Rice Genotypes

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Screening of Rice (Oryza sativa L.) Genotypes at Reproductive Stage for their Tolerance to Salinity

The Agriculturists ◽

10.3329/agric.v16i1.37535 ◽

2018 ◽

Vol 16 (1) ◽

pp. 65-77

Author(s):

M M Islam ◽

M H Faruqe ◽

M S Rana ◽

M Akter ◽

M A Karim

Keyword(s):

Oryza Sativa ◽

Salt Tolerance ◽

Grain Yield ◽

Oryza Sativa L ◽

Saline Water ◽

Coastal Zones ◽

Salt Tolerant ◽

Randomized Design ◽

Rice Genotypes ◽

Four Levels

Soil salinity is one of the most devastating environmental stresses for rice production in the coastal areas of Bangladesh. Improvement in salt tolerance of rice is an important way for the economic utilization of coastal zones. An experiment was conducted at the vinyl house of the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Bangladesh during December 2016 to April 2017 to determine the effects of different salinity levels on the yield and yield components of some rice (Oryza sativa L.) genotype sand finally, screening of rice genotypes for salt tolerance. The experiment containing four treatments was laid out in a complete randomized design with five replications. The treatments were four levels of saline water with electrical conductivities at control (0.3 dSm-1), 5, 10 and 15dSm-1. Based on screening at germination stage, relatively salt-tolerant eleven genotypes with two check varieties were used in this experiment. The study showed that increase in salinity level significantly decreased yield and yield contributing characters of rice. However, among the eleven, three genotypes (Chapali, Patnai 23 and Soloi) were considered as moderately salt tolerant at 15 dSm-1 on the basis of their yields and yield contributing characteristics such as plant height reduction, total tiller reduction, effective tiller reduction, reduction of fertile grains per panicle, grain yield and relative grain yield. Therefore, Chapali, Patnai 23 and Soloi might be recommended as moderately salt tolerant rice genotypes. The Agriculturists 2018; 16(1) 65-77

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