scholarly journals Mannitol metabolism in brown algae involves a new phosphatase family

2013 ◽  
Vol 65 (2) ◽  
pp. 559-570 ◽  
Author(s):  
Agnès Groisillier ◽  
Zhanru Shao ◽  
Gurvan Michel ◽  
Sophie Goulitquer ◽  
Patricia Bonin ◽  
...  
Keyword(s):  
Brown Algae ◽  
Mannitol Metabolism

scholarly journals Characterization of mannitol metabolism genes in Saccharina explains its key role in mannitol biosynthesis and evolutionary significance in Laminariales

2018 ◽  
Author(s):  
Shan Chi ◽  
Tao Liu ◽  
Cui Liu ◽  
Yuemei Jin ◽  
Hongxin Yin ◽  
...  
Keyword(s):  
Brown Algae ◽  
Molecular Mechanisms ◽  
Specific Activity ◽  
Expression Patterns ◽  
Dominant Gene ◽  
Biochemical Properties ◽  
Transcriptional Analysis ◽  
Evolutionary Significance ◽  
Mannitol Metabolism

AbstractAs a unique photosynthetic product in brown algae, mannitol exhibits high synthesis and accumulation in Saccharina japonica. Mannitol acts as a carbon-storage compound and is an osmoprotectant, imparting increased tolerance to osmotic stress. However, the underlying biochemical and molecular mechanisms in macroalgae have not been studied. Analysis of genomic and transcriptomic data has shown that mannitol metabolism in S. japonica is a circular pathway composed of four steps. In this study, one S. japonica mannitol-1-phosphate dehydrogenase (M1PDH2) and two mannitol-1-phosphatase (M1Pase) proteins were recombinantly expressed to analysis enzyme biochemical properties. RNA sequencing and droplet digital polymerase chain reaction were used to analyze the gene expression patterns of mannitol metabolism in different generations, tissues, sexes, and abiotic stresses. Our findings revealed insights into the mannitol synthesis pathways in brown algae. All genes were constitutively expressed in all samples, allowing maintenance of basic mannitol anabolism and dynamic maintenance of the “saccharide pool” in vivo as the main storage and antistress mechanism. Enzyme assays confirmed that the recombinant proteins produced mannitol, with the specific activity of SjaM1Pase1 being 1.8–4831 times that of other algal enzymes. Combined with the transcriptional analysis, SjaM1Pase1 was shown to be the dominant gene of mannitol metabolism. Mannitol metabolism genes in multicellular filamentous (gametophyte) and large parenchyma thallus (sporophyte) generations had different expression levels and responded differently under environmental stresses (hyposaline and hyperthermia) in gametophytes and sporophytes. The considerable variation in enzyme characteristics and expression of mannitol synthesis genes suggest their important ecophysiological significance in the evolution of complex systems (filamentous and thallus) and environmental adaptation of Laminariales.

Pyrolysis of brown algae (Bifurcaria Bifurcata) in a stainless steel tubular reactor: From a review to a case study

2018 ◽  
Vol 14 (1) ◽  
pp. 31-60 ◽  
Author(s):  
M. Y. Guida ◽  
F. E. Laghchioua ◽  
A. Hannioui
Keyword(s):  
Particle Size ◽  
Stainless Steel ◽  
Flow Rate ◽  
Brown Algae ◽  
Tubular Reactor ◽  
Nitrogen Flow ◽  
Nitrogen Flow Rate ◽  
Bifurcaria Bifurcata ◽  
Bio Oil

This article deals with fast pyrolysis of brown algae, such as Bifurcaria Bifurcata at the range of temperature 300–800 °C in a stainless steel tubular reactor. After a literature review on algae and its importance in renewable sector, a case study was done on pyrolysis of brown algae especially, Bifurcaria Bifurcata. The aim was to experimentally investigate how the temperature, the particle size, the nitrogen flow rate (N2) and the heating rate affect bio-oil, bio-char and gaseous products. These parameters were varied in the ranges of 5–50 °C/min, below 0.2–1 mm and 20–200 mL. min–1, respectively. The maximum bio-oil yield of 41.3wt% was obtained at a pyrolysis temperature of 600 °C, particle size between 0.2–0.5 mm, nitrogen flow rate (N2) of 100 mL. min–1 and heating rate of 5 °C/min. Liquid product obtained under the most suitable and optimal condition was characterized by elemental analysis, 1H-NMR, FT-IR and GC-MS. The analysis of bio-oil showed that bio-oil from Bifurcaria Bifurcata could be a potential source of renewable fuel production and value added chemicals.

scholarly journals CHLOROFUCINE (CHLOROPHYLL γ), A GREEN PIGMENT OF DIATOMS AND BROWN ALGAE

1942 ◽  
Vol 144 (3) ◽  
pp. 625-636
Author(s):  
Harold H. Strain ◽  
Winston M. Manning
Keyword(s):  
Brown Algae ◽  
Green Pigment

scholarly journals In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects

Marine Drugs ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 34
Author(s):  
Mélody Dutot ◽  
Elodie Olivier ◽  
Sophie Fouyet ◽  
Romain Magny ◽  
Karim Hammad ◽  
...  
Keyword(s):  
Brown Algae ◽  
P2x7 Receptor ◽  
Biological Activities ◽  
Receptor Activation ◽  
Cytotoxic Effects ◽  
Human Lung Cells ◽  
Cytochrome P450 Activity ◽  
Species Production ◽  
E Cadherin

Phlorotannins are polyphenols occurring exclusively in some species of brown algae, known for numerous biological activities, e.g., antioxidant, antiproliferative, antidiabetic, and antiallergic properties. Their effects on the response of human lung cells to benzo[a]pyrene (B[a]P) has not been characterized. Our objective was to in vitro evaluate the effects of a phlorotannin-rich extract obtained from the brown algae Ascophyllum nodosum and Fucus vesiculosus on B[a]P cytotoxic effects. The A549 cell line was incubated with B[a]P for 48 and 72 h in the presence or absence of the brown algae extract. Cytochrome P450 activity, activation of P2X7 receptor, F-actin disorganization, and loss of E-cadherin expression were assessed using microplate cytometry and fluorescence microscopy. Relative to control, incubation with the brown algae extract was associated with lower B[a]P-induced CYP1 activity, lower P2X7 receptor activation, and lower reactive oxygen species production. The brown algae extract inhibited the alterations of F-actin arrangement and the downregulation of E-cadherin expression. We identified a phlorotannins-rich extract that could be deeper investigated as a cancer chemopreventive agent to block B[a]P-mediated carcinogenesis.

scholarly journals Evolution of life cycles and reproductive traits: insights from the brown algae

2021 ◽  
Author(s):  
Svenja Heesch ◽  
Martha Serrano‐Serrano ◽  
Josué Barrera‐Redondo ◽  
Rémy Luthringer ◽  
Akira F. Peters ◽  
...  
Keyword(s):  
Brown Algae ◽  
Reproductive Traits ◽  
Life Cycles ◽  
Evolution Of Life

In vitro biological activities and in vivo hepatoprotective role of brown algae-isolated fucoidans

2021 ◽  
Author(s):  
Marwa E. Atya ◽  
Amr El-Hawiet ◽  
Mohamed A. Alyeldeen ◽  
Doaa A. Ghareeb ◽  
Mohamed M. Abdel-Daim ◽  
...  
Keyword(s):  
Brown Algae ◽  
Biological Activities
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