scholarly journals Transcript and metabolite changes during the early phase of abscisic acid-mediated induction of crassulacean acid metabolism in Talinum triangulare

2019 ◽  
Vol 70 (22) ◽  
pp. 6581-6596 ◽  
Author(s):  
Eva Maleckova ◽  
Dominik Brilhaus ◽  
Thomas J Wrobel ◽  
Andreas P M Weber
Keyword(s):  
Abscisic Acid ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Enrichment Analysis ◽  
Titratable Acidity ◽  
Abscisic Acid Metabolism ◽  
Use Efficiency ◽  
Cam Regulation ◽  
Cam Species ◽  
Talinum Triangulare

Abstract Crassulacean acid metabolism (CAM) has evolved as a water-saving strategy, and its engineering into crops offers an opportunity to improve their water use efficiency. This requires a comprehensive understanding of the regulation of the CAM pathway. Here, we use the facultative CAM species Talinum triangulare as a model in which CAM can be induced rapidly by exogenous abscisic acid. RNA sequencing and metabolite measurements were employed to analyse the changes underlying CAM induction and identify potential CAM regulators. Non-negative matrix factorization followed by k-means clustering identified an early CAM-specific cluster and a late one, which was specific for the early light phase. Enrichment analysis revealed abscisic acid metabolism, WRKY-regulated transcription, sugar and nutrient transport, and protein degradation in these clusters. Activation of the CAM pathway was supported by up-regulation of phosphoenolpyruvate carboxylase, cytosolic and chloroplastic malic enzymes, and several transport proteins, as well as by increased end-of-night titratable acidity and malate accumulation. The transcription factors HSFA2, NF-YA9, and JMJ27 were identified as candidate regulators of CAM induction. With this study we promote the model species T. triangulare, in which CAM can be induced in a controlled way, enabling further deciphering of CAM regulation.

Carbon isotope composition and water-use efficiency in plants with crassulacean acid metabolism

2005 ◽  
Vol 32 (5) ◽  
pp. 381 ◽  
Author(s):  
Klaus Winter ◽  
Jorge Aranda ◽  
Joseph A. M. Holtum
Keyword(s):  
Water Use Efficiency ◽  
Water Use ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Aloe Vera ◽  
Dry Mass ◽  
Tropical Conditions ◽  
Δ13c Value ◽  
Use Efficiency ◽  
Cam Species

The relationship between water-use efficiency, measured as the transpiration ratio (g H2O transpired g–1 above- plus below-ground dry mass accumulated), and 13C / 12C ratio (expressed as δ13C value) of bulk biomass carbon was compared in 15 plant species growing under tropical conditions at two field sites in the Republic of Panama. The species included five constitutive crassulacean acid metabolism (CAM) species [Aloe vera (L.) Webb & Berth., Ananas comosus (L.) Merr., Euphorbia tirucalli L., Kalanchoë daigremontiana Hamet et Perr., Kalanchoë pinnata (Lam.) Pers.], two species of tropical C3 trees (Tectona grandis Linn. f. and Swietenia macrophylla King), one C4 species (Zea mays L.), and seven arborescent species of the neotropical genus Clusia, of which two exhibited pronounced CAM. The transpiration ratios of the C3 and CAM species, which ranged between 496 g H2O g–1 dry mass in the C3–CAM species Clusia pratensis Seeman to 54 g H2O g–1 dry mass in the constitutive CAM species Aloe vera, correlated strongly with δ13C values and nocturnal CO2 gain suggesting that δ13C value can be used to estimate both water-use efficiency and the proportion of CO2 gained by CAM species during the light and the dark integrated over the lifetime of the tissues.

scholarly journals Exploring the Relationship between Crassulacean Acid Metabolism (CAM) and Mineral Nutrition with a Special Focus on Nitrogen

2019 ◽  
Vol 20 (18) ◽  
pp. 4363 ◽  
Author(s):  
Paula Natália Pereira ◽  
John C. Cushman
Keyword(s):  
Water Use Efficiency ◽  
Water Use ◽  
Nutrient Availability ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Special Focus ◽  
Cam Plants ◽  
Use Efficiency ◽  
Cam Species ◽  
The Relationship

Crassulacean acid metabolism (CAM) is characterized by nocturnal CO2 uptake and concentration, reduced photorespiration, and increased water-use efficiency (WUE) when compared to C3 and C4 plants. Plants can perform different types of CAM and the magnitude and duration of CAM expression can change based upon several abiotic conditions, including nutrient availability. Here, we summarize the abiotic factors that are associated with an increase in CAM expression with an emphasis on the relationship between CAM photosynthesis and nutrient availability, with particular focus on nitrogen, phosphorus, potassium, and calcium. Additionally, we examine nitrogen uptake and assimilation as this macronutrient has received the greatest amount of attention in studies using CAM species. We also discuss the preference of CAM species for different organic and inorganic sources of nitrogen, including nitrate, ammonium, glutamine, and urea. Lastly, we make recommendations for future research areas to better understand the relationship between macronutrients and CAM and how their interaction might improve nutrient and water-use efficiency in order to increase the growth and yield of CAM plants, especially CAM crops that may become increasingly important as global climate change continues.

Abscisic acid metabolism during development and maturation of rambutan fruit

2001 ◽  
Vol 76 (2) ◽  
pp. 235-241 ◽  
Author(s):  
S.K. Satoru Kondo ◽  
P.P. Panumas Posuya ◽  
S.K. Sirichai Kanlayanarat ◽  
N.H. Nobuhiro Hirai
Keyword(s):  
Abscisic Acid ◽  
Acid Metabolism ◽  
Abscisic Acid Metabolism

scholarly journals Salinity induction of recycling Crassulacean acid metabolism and salt tolerance in plants of Talinum triangulare

2018 ◽  
Vol 121 (7) ◽  
pp. 1333-1342 ◽  
Author(s):  
Estefanía Montero ◽  
Ana Marta Francisco ◽  
Enrique Montes ◽  
Ana Herrera
Keyword(s):  
Salt Tolerance ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Talinum Triangulare

Degrees of crassulacean acid metabolism in tropical epiphytic and lithophytic ferns

1999 ◽  
Vol 26 (8) ◽  
pp. 749 ◽  
Author(s):  
Joseph A.M. Holtum ◽  
Klaus Winter
Keyword(s):  
Carbon Isotope ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Dark Period ◽  
Titratable Acidity ◽  
Isotope Ratios ◽  
Light Period ◽  
Co2 Uptake ◽  
Carbon Isotope Ratios ◽  
Net Co2 Uptake

Crassulacean acid metabolism (CAM) was observed in three species of tropical ferns, the epiphytes Microsorium punctatum and Polypodium crassifolium and the lithophyte Platycerium veitchii. Polypodium crassifolium and P. veitchii exhibited characteristics of weak CAM. Although no net nocturnal CO2 uptake was observed, the presence of CAM was inferred from nocturnal increases in titratable acidity of 4.7 and 4.1 µequiv (g fr wt)–1 respectively, a reduction in the rates of net CO2 evolution during the first half of the dark period, and the presence of a CAM-like decrease in net CO2 uptake during the early light period. In M. punctatum net CO2 uptake during the first half of the dark period was accompanied by an increase in titratable acidity of 39.2 µequiv (g fr wt)–1 and a pronounced reduction in net CO2 uptake during the early light period. When water was withheld from P. crassifolium and M. punctatum, net CO2 uptake during the light was reduced markedly but there was no change in the extent or patterns of CO2 exhange in the dark. As a consequence, the proportion of carbon gained due to CO2 fixation in the dark increased from 2.8 and 10% to 63.5 and 49.3%, respectively (100% being net CO2 uptake during the light plus the estimated CO2 uptake during the dark). After 9 days without added water, dark CO2 uptake was responsible for the maintenance of a net 24 h carbon gain in P. crassifolium. Platycerium veitchii, P. crassifolium and M. punctatum exhibited carbon isotope ratios of between –25.9 and –22.6‰ indicating that carbon isotope ratios may not, by themselves, be sufficient for the identification of weak CAM. We suggest that CAM may be more prevalent in tropical epiphytic and lithophytic ferns than currently envisaged.

scholarly journals A perspective on crassulacean acid metabolism photosynthesis evolution of orchids on different continents: Dendrobium as a case study

2019 ◽  
Vol 70 (22) ◽  
pp. 6611-6619
Author(s):  
Ming-He Li ◽  
Ding-Kun Liu ◽  
Guo-Qiang Zhang ◽  
Hua Deng ◽  
Xiong-De Tu ◽  
...  
Keyword(s):  
Crassulacean Acid Metabolism ◽  
Middle Miocene ◽  
Acid Metabolism ◽  
Flowering Plants ◽  
Asian Clade ◽  
Wide Range ◽  
Cam Species ◽  
Climatic Cooling ◽  
Cam Photosynthesis

Abstract Members of the Orchidaceae, one of the largest families of flowering plants, evolved the crassulacean acid metabolism (CAM) photosynthesis strategy. It is thought that CAM triggers adaptive radiation into new niche spaces, yet very little is known about its origin and diversification on different continents. Here, we assess the prevalence of CAM in Dendrobium, which is one of the largest genera of flowering plants and found in a wide range of environments, from the high altitudes of the Himalayas to relatively arid habitats in Australia. Based on phylogenetic time trees, we estimated that CAM, as determined by δ 13C values less negative than –20.0‰, evolved independently at least eight times in Dendrobium. The oldest lineage appeared in the Asian clade during the middle Miocene, indicating the origin of CAM was associated with a pronounced climatic cooling that followed a period of aridity. Divergence of the four CAM lineages in the Asian clade appeared to be earlier than divergence of those in the Australasian clade. However, CAM species in the Asian clade are much less diverse (25.6%) than those in the Australasian clade (57.9%). These findings shed new light on CAM evolutionary history and the aridity levels of the paleoclimate on different continents.

Sign in / Sign up

Export Citation Format

Share Document