scholarly journals All roads lead to growth: imaging-based and biochemical methods to measure plant growth

2019 ◽  
Vol 71 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Justyna Jadwiga Olas ◽  
Franziska Fichtner ◽  
Federico Apelt

We provide a comprehensive description and comparison of the various imaging and non-imaging-based techniques used to measure vegetative plant shoot growth and suggest a terminology for growth rates.

1962 ◽  
Vol 10 (4) ◽  
pp. 286-296 ◽  
Author(s):  
H. Hoestra ◽  
M. Oostenbrink

The damage caused by Pratylenchus penetrans in orchards is discussed. In 2 experimental fields containing 4 varieties of apple, there was good evidence of a decrease in yield with increased nematode density before apple seedlings were planted. Heavy nematode infestations reduced shoot growth by more than 50%. A concentration of 100 nematodes per 300 ml. of soil may cause considerable damage. The process of infestation and symptoms of nematode attack under field and experimental conditions are discussed. In clean cultivated orchards on light sandy soils there are often large populations in the roots and very small populations in the soil but on heavier soils, the converse is true. Hoestra & Oostenbrink conclude without doubt that P. penetrans is an important cause of replant problems in orchards. H.R. W. (Abstract retrieved from CAB Abstracts by CABI’s permission)


2003 ◽  
Vol 2 (2) ◽  
pp. 97-100 ◽  
Author(s):  
Manabu Watanabe ◽  
Akira Suzuki ◽  
Sadao Komori ◽  
Hidetugu Sato

1999 ◽  
Vol 50 (2) ◽  
pp. 217 ◽  
Author(s):  
D. O. Huett ◽  
S. C. Morris

Nutrient leaching loss, plant growth, and nutrient uptake of 4-week (transplanting to sale) ground-cover species were investigated under a range of leaching conditions and with different sources of a controlled- release fertiliser (CRF), Osmocote NPK (3–4 month) (Osm). Osm was applied pre-planting at a rate equivalent to 800 g N/m3 to pots containing sand, and composted pinebark and hardwood sawdust medium that had received nutrient amendment during formulation. Two experiments were conducted in a glasshouse over summer–autumn where irrigation treatments produced defined leachate volumes. In Expt 1, leachate volumes of <5, 50, and 200 mL every 2 days each received an additional single heavy leaching event of 400 mL after 1, 2, or 3 weeks. In Expt 2, the 3 leachate volumes were each fertilised with new Osm (a newly purchased Osm) or old Osm (a 2-year-old source), where both of these sources contained 0.5–1.5% visibly damaged prills; and damaged Osm, where damaged prills were used exclusively. In both experiments, increasing leachate volume increased (P < 0.001) leaching of N (nitrate + ammonium), P, K, Ca, and Mg. In Expt 1, leaching was highest (P < 0.01) when the heavy leaching event occurred after 2 or 3 weeks for N and after 2 weeks for P. When damaged Osm was used, N, P, and K loss was 3–15 times higher (P < 0.001) than from new and old Osm (98.5–99.5% undamaged). The highest leaching loss of N, P, K, Ca, and Mg occurred in the first week after potting up, with damaged prills at highest leaching volume. Increasing leachate volume (in the presence of a heavy leaching event) reduced (P < 0.001) electrical conductivity (EC) of potting medium after 4 weeks from 1.02 to 0.54 dS/m. Damaged prills reduced (P < 0.001) EC at the high leachate volume in relation to new Osm (2.38 v. 0.29 dS/m). Treatments that increased (P < 0.05) nutrient leaching generally reduced (P < 0.05) nutrient concentrations in shoots and depressed the growth of some plant species. Shoot growth of 2 of 5 species was reduced (P < 0.001) at the highest leachate volume with an additional heavy leaching event in Week 1 or 2, and root growth of all but the slowest growing species declined with increasing leachate volume. Damaged prills reduced (P < 0.001) shoot growth of 2 of the 5 ground-cover species. This study demonstrated that excessive leaching and the use of damaged prills for containerised nursery plants fertilised with CRF results in high nutrient loss, low residual nutrient content, reduced nutrient uptake in shoots, and reduced shoot growth of some species.


1998 ◽  
Vol 38 (4) ◽  
pp. 385
Author(s):  
R. F. Brennan ◽  
A. M. Crowhurst ◽  
M. G. Webb

Summary. Native plants are increasingly grown in Western Australia to produce flowers for export. The nitrogen (N), phosphorus (P) and potassium (K) requirements for optimum production of one of these species, Geleznowia verrucosa (Yellow Bells), was measured for 17-week-old seedlings in a glasshouse experiment reported here. There was a significant (P<0.05) growth response to all levels of N fertilisers. At all levels of P and K, except for the nil K treatments, the lowest level of applied N (20 mg N/kg soil) gave the maximum dry weight of shoots. The dry weight of shoots increased with the addition of P fertiliser to the highest level (160 mg P/kg soil), particularly for the lower levels of applied K (0 and 30 mg/kg soil) and the lowest level of applied N (20 mg/kg soil). Combinations of high levels of P (P160) and N (N80) fertiliser severely depressed shoot growth. When applied at greater than 30 mg K/kg soil, K fertiliser depressed plant growth at all levels of N and P when compared with the lower levels of applied K. At the seedling stage of growth, critical concentrations for deficiency of both N and K were 1.3% in shoots. The critical concentrations for toxicity in whole shoots of Yellow Bells appeared to be about 1.7% for N and about 2.2% for K. Adequate concentrations of N were 1.4–1.5%, while 1.7% K appeared adequate for growth of Yellow Bell shoots.


1990 ◽  
Vol 17 (4) ◽  
pp. 451 ◽  
Author(s):  
FW Smith ◽  
WA Jackson ◽  
PJV Berg

Partitioning and net transfer of phosphorus between shoots and roots in the tropical forage legume Stylosanthes hamata cv. Verano during the development of phosphorus deficiency has been studied. Plants were stressed by either growing them in dilute flowing culture on continuously maintained external phosphorus concentrations that were inadequate for maximal growth, or by transferring plants of varying phosphorus status to phosphorus-free media. An external phosphorus concentration of 1 �M P was found to be just adequate for maximal growth of S. hamata. Phosphorus stress caused rapid and substantial increases in root weight percentage. It is proposed that this represents an important adaptive mechanism for maximising phosphorus uptake by S. hamata growing in phosphorus-deficient soils. Roots contained the minimum proportion of the plant's phosphorus content when root phosphorus concentrations were 8-10 �mol P g-1 root, and shoot phosphorus concentrations were 16-20 �mol P g-1 shoot. When tissue concentrations were less than these values, plants suffered from phosphorus stress and phosphorus was either preferentially retained by the roots or rapidly transferred from shoots to roots, reducing the growth rates of shoots, but permitting root growth to continue. Upon reducing the external phosphorus supply to plants whose root phosphorus concentrations exceeded 8 to 10 �mol P g-1 root, excess phosphorus was rapidly transferred from the root to the shoot to maintain shoot growth rates. The mobility of phospborus within the plant, and the apparent lack of any delay in transferring phosphorus from shoots to roots as phosphorus stress developed, represent another adaptive feature that is likely to be important to the successful growth of S. hamata in low phosphorus soils. When the phosphorus supply was limited, the plant's resources were directed toward maintaining root growth. Even extremely phosphorus deficient plants, in which shoot growth had ceased, maintained linear rates of root growth. These linear rates were related to the total phosphorus content of the plant. In the latter stages of phosphorus deprivation, linear rates of root growth were maintained by remobilisation of phosphorus from the older parts of the root system to sustain the phosphorus supply to the root meristems.


Weed Science ◽  
1970 ◽  
Vol 18 (6) ◽  
pp. 692-696 ◽  
Author(s):  
J. Deli ◽  
G. F. Warren

Root application ofN,N-dimethyl-2,2-diphenylacetamide (diphenamid) caused reduction of root and shoot growth of oats (Avena sativaL., var. Jaycee) seedlings. Shoot application did not affect plant growth, but studies with labeled diphenamid showed that diphenamid will enter also through the shoot. In ivyleaf morningglory (Ipomoea hederaceaL.), a considerable amount of label was translocated from the roots to the shoots, but not in oats seedlings. The difference in tolerance between these two species (oats susceptible, morningglory resistant) may lie in the ability of morningglory to translocate diphenamid out of the roots into the shoots faster than oats. The inhibitory effect of diphenamid was restricted to the site of uptake. Reduction in shoot growth of treated plants was the result of the limited root system and it was not a direct effect of diphenamid. Diphenamid was 10 times as toxic to oats as its metabolites. Oats seedlings inhibited by diphenamid for up to 5 days, and then placed in water recovered from the diphenamid caused inhibition. The resumed root growth appeared to be normal. The amount of uptake of14C-labeled sucrose by excised roots treated with 10−5M diphenamid was equal to that in untreated roots; however, more sugar was incorporated into the untreated roots than the treated roots. It appears that diphenamid is a reversible metabolic inhibitor; it inhibits cell division in the root tip perhaps by limiting utilization of substrates in the cells.


2016 ◽  
Vol 43 (8) ◽  
pp. 739 ◽  
Author(s):  
Louis Moir-Barnetson ◽  
Erik J. Veneklaas ◽  
Timothy D. Colmer

We evaluated tolerances to salinity (10–2000 mM NaCl) in three halophytic succulent Tecticornia species that are differentially distributed along a salinity gradient at an ephemeral salt lake. The three species showed similar relative shoot and root growth rates at 10–1200 mM NaCl; at 2000 mM NaCl, T. indica subsp. bidens (Nees) K.A.Sheph and P.G.Wilson died, but T. medusa (K.A.Sheph and S.J.van Leeuwen) and T. auriculata (P.G.Wilson) K.A.Sheph and P.G.Wilson survived but showed highly diminished growth rates and were at incipient water stress. The mechanisms of salinity tolerance did not differ among the three species and involved the osmotic adjustment of succulent shoot tissues by the accumulation of Na+, Cl– and the compatible solute glycinebetaine, and the maintenance of high net K+ to Na+ selectivity to the shoot. Growth at extreme salinity was presumably limited by the capacity for vacuolar Na+ and Cl– uptake to provide sufficiently low tissue osmotic potentials for turgor-driven growth. Tissue sugar concentrations were not reduced at high salinity, suggesting that declines in growth would not have been caused by inadequate photosynthesis and substrate limitation compared with plants at low salinity. Equable salt tolerance among the three species up to 1200 mM NaCl means that other factors are likely to contribute to species composition at sites with salinities below this level. The lower NaCl tolerance threshold for survival in T. indica suggests that this species would be competitively inferior to T. medusa and T. auriculata in extremely saline soils.


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