PIF3 Is Involved in the Primary Root Growth Inhibition of Arabidopsis Induced by Nitric Oxide in the Light

Abstract Boron (B) is an essential micronutrient for plant growth and development. Jasmonic acid (JA) plays pivotal roles in plant growth. However, the underlying molecular mechanism of JA involvement in B-deficiency-induced root growth inhibition is yet to be explored. In this study, we investigated the response of JA to B deficiency and the mechanism of JAR1-dependent JA signaling in root growth inhibition under B deficiency. B deficiency enhanced JA signaling in roots, and root growth inhibition was partially restored by JA biosynthesis inhibition. jar1-1 (jasmonate-resistant 1, JAR1) mutant, mutants of coronatine-insensitive 1 (coi1-2) and myc2 defective in JA signaling showed insensitivity to B deficiency. Ethylene-overproduction mutant eto1 and ethylene-insensitive mutant etr1 showed sensitivity and insensitivity to B deficiency, respectively, suggesting that ethylene is involved in the inhibition of primary root growth under B deficiency. Furthermore, after a declined in EIN3 protein levels, which may contribute to root growth, ethylene signaling was weakened in the jar1-1 mutant root under B deficiency. Under B deficiency, B concentrations were increased in the roots and shoots of the jar1-1 mutant, owing to the large root system and its activity. Therefore, our findings revealed that JA, which is involved in the inhibition of root growth under B deficiency, is regulated by JAR1 activated JA and ethylene signaling pathways.

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Exogenous Nitro-Oleic Acid inhibits primary root growth by reducing the mitosis in the meristem in Arabidopsis thaliana

10.1101/2020.06.17.155416 ◽

2020 ◽

Author(s):

Luciano M. Di Fino ◽

Ignacio Cerrudo ◽

Sonia R. Salvatore ◽

Francisco J. Schopfer ◽

Carlos García-Mata ◽

...

Keyword(s):

Nitric Oxide ◽

Fatty Acids ◽

Oleic Acid ◽

Root Growth ◽

Unsaturated Fatty Acids ◽

Primary Root ◽

Cell Number ◽

No Production ◽

Physiological Processes ◽

Primary Root Growth

ABSTRACTNitric oxide (NO) is a second messenger that regulates a broad range of physiological processes in plants. NO-derived molecules called reactive nitrogen species (RNS) can react with unsaturated fatty acids generating nitrated fatty acids (NO2-FA). NO2-FA work as signaling molecules in mammals where production and targets have been described under different stress conditions. Recently, NO2-FAs were detected in plants, however their role(s) on plant physiological processes is still poorly known. Here we show that exogenous application of nitro-oleic acid (NO2-OA) inhibits Arabidopsis primary root growth; this inhibition is not likely due to nitric oxide (NO) production or impaired auxin or cytokinin root responses. Deep analyses showed that roots incubated with NO2-OA had a lower cell number in the division area. Although this NO2-FA did not affect the signaling mechanisms maintaining the stem cell niche, plants incubated with NO2-OA showed a reduction of cell division in the meristematic area. Therefore, this work shows that NO2-OA inhibits mitotic processes subsequently reducing primary root growth.

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Melatonin alleviates aluminum-induced root growth inhibition by interfering with nitric oxide production in Arabidopsis

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2018.08.014 ◽

2019 ◽

Vol 161 ◽

pp. 157-165 ◽

Cited By ~ 21

Author(s):

Jiarong Zhang ◽

Dongxu Li ◽

Jian Wei ◽

Wenna Ma ◽

Xiangying Kong ◽

...

Keyword(s):

Nitric Oxide ◽

Root Growth ◽

Growth Inhibition ◽

Root Growth Inhibition ◽

Nitric Oxide Production ◽

Oxide Production

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Interactions between nitric oxide, gibberellic acid, and phosphorus regulate primary root growth in Arabidopsis

Biologia Plantarum ◽

10.1007/s10535-014-0408-7 ◽

2014 ◽

Vol 58 (2) ◽

pp. 335-340 ◽

Cited By ~ 15

Author(s):

A. P. Wu ◽

L. Gong ◽

X. Chen ◽

J. X. Wang

Keyword(s):

Nitric Oxide ◽

Gibberellic Acid ◽

Root Growth ◽

Primary Root ◽

Primary Root Growth

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Jasmonate Signalling Contributes to Primary Root Inhibition Upon Oxygen Deficiency in Arabidopsis thaliana

Plants ◽

10.3390/plants9081046 ◽

2020 ◽

Vol 9 (8) ◽

pp. 1046 ◽

Cited By ~ 1

Author(s):

Vinay Shukla ◽

Lara Lombardi ◽

Ales Pencik ◽

Ondrej Novak ◽

Daan A. Weits ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Root Growth ◽

Growth Inhibition ◽

Feedback Inhibition ◽

Oxygen Sensing ◽

Oxygen Deficiency ◽

Primary Root ◽

Root Growth Inhibition ◽

Root Inhibition ◽

Active Root

Plants, including most crops, are intolerant to waterlogging, a stressful condition that limits the oxygen available for roots, thereby inhibiting their growth and functionality. Whether root growth inhibition represents a preventive measure to save energy or is rather a consequence of reduced metabolic rates has yet to be elucidated. In the present study, we gathered evidence for hypoxic repression of root meristem regulators that leads to root growth inhibition. We also explored the contribution of the hormone jasmonic acid (JA) to this process in Arabidopsis thaliana. Analysis of transcriptomic profiles, visualisation of fluorescent reporters and direct hormone quantification confirmed the activation of JA signalling under hypoxia in the roots. Further, root growth assessment in JA-related mutants in aerobic and anaerobic conditions indicated that JA signalling components contribute to active root inhibition under hypoxia. Finally, we show that the oxygen-sensing transcription factor (TF) RAP2.12 can directly induce Jasmonate Zinc-finger proteins (JAZs), repressors of JA signalling, to establish feedback inhibition. In summary, our study sheds new light on active root growth restriction under hypoxic conditions and on the involvement of the JA hormone in this process and its cross talk with the oxygen sensing machinery of higher plants.

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