scholarly journals Exploration of intramolecular split G-quadruplex and its analytical applications

2019 ◽  
Vol 47 (18) ◽  
pp. 9502-9510 ◽  
Author(s):  
Mengmeng Lv ◽  
Yuchun Guo ◽  
Jiangtao Ren ◽  
Erkang Wang
Keyword(s):  
Melting Temperature ◽  
Thermodynamic Stability ◽  
Spacer Length ◽  
Effective Application ◽  
Comprehensive Information ◽  
G Quadruplex ◽  
Split Mode ◽  
Dna Technology ◽  
Bioanalytical Applications ◽  
Selection Of

Abstract Distinct from intermolecular split G-quadruplex (Inter-SG), intramolecular split G-quadruplex (Intra-SG) which could be generated in a DNA spacer-inserted G-quadruplex strand has not been systematically explored. Not only is it essential for the purpose of simplicity of DNA-based bioanalytical applications, but also it will give us hints how to design split G-quadruplex-based system. Herein, comprehensive information is provided about influences of spacer length and split mode on the formation of Intra-SG, how to adjust its thermodynamic stability, and selection of optimal Intra-SG for bioanalysis. For instances, non-classical Intra-SG (e.g. 2:10, 4:8 and 5:7) displays lower stability than classical split strands (3:9, 6:6 and 9:3), which is closely related to integrity of consecutive guanine tract; as compared to regular Intra-SG structures, single-thymine capped ones have reduced melting temperature, providing an effective approach to adjustment of stability. It is believed that the disclosed rules in this study will contribute to the effective application of split G-quadruplex in the field of DNA technology in the future.

scholarly journals The changes of LD-PE films after exposure in different media

2020 ◽  
Vol 26 (4) ◽  
pp. 185-189
Author(s):  
Tatiana Kojnoková ◽  
Lenka Markovičová ◽  
František Nový
Keyword(s):  
Water Absorption ◽  
Melting Temperature ◽  
Aggressive Medium ◽  
Polyethylene Film ◽  
Low Density Polyethylene ◽  
Low Density ◽  
Experimental Part ◽  
Effective Application ◽  
Suitable Medium ◽  
Selection Of

AbstractThis work deals with the study of polymers, and, in particular, polyethylene; its production, types, properties, and usage. The experimental part evaluates the changes of properties of the polyethylene film to be reused under various exposure conditions and selection of the most suitable medium for its application. The film made of low-density polyethylene (LD-PE) was influenced by aggressive media with different pH, specifically Savo for the disinfection, Savo as a Saponate for dish washing and Coca-Cola. On LD-PE films the water absorption and melting temperature evaluation tests were performed. Carried out tests show that the most aggressive medium for LD-PE film from used media is Coca-Cola. The most effective application of LD-PE film like wrapping on container transported is the Savo used as a Saponate for dish washing.

Electrochemical selection of G-quadruplex-binding ligands based on structure-switching of telomeric DNA

2011 ◽  
Vol 3 (6) ◽  
pp. 1270 ◽  
Author(s):  
Xiao-Qin Liu ◽  
Yan Jin ◽  
Yuexia Wang ◽  
Yunxia Qiao
Keyword(s):  
Telomeric Dna ◽  
G Quadruplex ◽  
Structure Switching ◽  
Selection Of

Selection of G-Quadruplex Folding Topology with LNA-Modified Human Telomeric Sequences in K+Solution

2011 ◽  
Vol 17 (8) ◽  
pp. 2405-2413 ◽  
Author(s):  
Devranjan Pradhan ◽  
Lykke H. Hansen ◽  
Birte Vester ◽  
Michael Petersen
Keyword(s):  
Telomeric Sequences ◽  
G Quadruplex ◽  
Selection Of

scholarly journals Haplotypes for Type, Degree, and Rate of Marbling in Cattle Are Syntenic with Human Muscular Dystrophy

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Sally S. Lloyd ◽  
Edward J. Steele ◽  
Jose L. Valenzuela ◽  
Roger L. Dawkins
Keyword(s):  
Muscular Dystrophy ◽  
Melting Temperature ◽  
Muscle Metabolism ◽  
Intramuscular Fat ◽  
Climatic Conditions ◽  
Alternative Approach ◽  
Selection Of

Traditional analyses of a QTL on Bota 19 implicate a surfeit of candidates, but each is of marginal significance in explaining the deposition of healthy, low melting temperature fat within marbled muscle of Wagyu cattle. As an alternative approach, we have used genomic, multigenerational segregation to identify 14 conserved, ancestral 20 Mb haplotypes. These determine the degree and rate of marbling in Wagyu and other breeds of cattle. The melting temperature of intramuscular fat is highly heritable and traceable by haplotyping. Fortunately, for the production of healthy beef, some of these haplotypes are sufficiently penetrant to be expressed in heterozygous crossbreds, thereby allowing selection of sires which will improve the healthiness of beef produced under even harsh climatic conditions. The region of Bota 19 is syntenic to a region of Hosa 17 known to be important in muscle metabolism and in determining susceptibility to a form of human muscular dystrophy.

scholarly journals Thermodynamic stability of G-quadruplex and overlapping m6A: Position-dependent collaborators in allele frequency and fitness?

2021 ◽  
Author(s):  
Cagri Gulec
Keyword(s):  
Statistical Analysis ◽  
Allele Frequency ◽  
Gene Product ◽  
Thermodynamic Stability ◽  
Rna Processing ◽  
Genetic Variants ◽  
Common Variants ◽  
Unequal Distribution ◽  
Human Genes ◽  
G Quadruplex

AbstractBackgroundPost-transcriptional modifications like m6A, and secondary structures like G-quadruplex (G4), play an important role in RNA processing. Despite an emerging number of studies focusing on m6A and G4 separately, there are less studies about their synergy.AimSince m6A is known to be enzymatically created in DRACH-motif, and genetic variants may create a novel DRACH-motif or abolish a pre-existing DRACH-motif, we can suppose that the variants may affect gene product level through modulating m6A-G4 colocalization, which consequently may affect fitness and change allele frequency. To test this hypothesis, rare and common variants in selected human genes were investigated in terms of their effect on m6A-G4 colocalization.MethodsGenomic sequences and variant features were fetched from GRCh37/hg19 and Biomart-Ensembl databases, respectively. Counting the number of putative m6A- and G4-motifs in sequences and statistical analysis were performed with appropriate libraries of Python3.7.ResultsCommon variants creating novel m6A-motif were found more frequently inside than outside G4, and displayed unequal distribution throughout pre-mRNA. Unequal distribution of m6A-creating variants seemed to be related to their effect on thermodynamic stability of the overlapping-G4.DiscussionSelective m6A-G4 colocalization suggests that m6A-motif is favorable when overlapping with G4. Besides, thermodynamic stability may lead to unequal distribution of m6A-G4 colocalization, because m6A-creating alleles seem to have lower frequency if stabilizes overlapping-G4 in 3-prime-side, but not in 5-prime-side. We can conclude that the fitness, and consequently frequency of an m6A-creating variant is prone to become higher or lower depending on its position and effect on the overlapping-G4 stability.

scholarly journals Inquisitorial: Menelusuri Titik Terminal Keberangkatan Ilmu Pemerintahan Di Indonesia

ARISTO ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 176
Author(s):  
T. Fahrul Gafar ◽  
Zamhasari Jamil
Keyword(s):  
Growth And Development ◽  
Qualitative Approach ◽  
Library Research ◽  
Comprehensive Information ◽  
Selection Of

This research is a scientific effort to trace the historical footsteps of the birth, growth, and development of Government Science in Indonesia. The disclosure of the early phenomenon of science would certainly be the gateway in giving a pure understanding of that science. so that, the result of its inquiry can serve as one of the main sources/ references that provide complete and comprehensive information for further study of Government Science. This study used a qualitative approach with descriptive type and with the library research form. In this study, the researcher observed the object of research through literature materials so that the theories underlying the problems in the field under study can be clearly revealed. With the selection of approaches, types, and forms of research, will certainly be able to reveal in depth how the historical roots of Government Science and able to emphasize the locus / position of Government Science among other state sciences.

scholarly journals Short Duplex Module Coupled to G-Quadruplexes Increases Fluorescence of Synthetic GFP Chromophore Analogues

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 915
Author(s):  
Snizhana O. Zaitseva ◽  
Nadezhda S. Baleeva ◽  
Timofei S. Zatsepin ◽  
Ivan N. Myasnyanko ◽  
Anton V. Turaev ◽  
...  
Keyword(s):  
Bioanalytical Chemistry ◽  
G Quadruplex ◽  
Dye Binding ◽  
Binding Cavity ◽  
Fluorescence Light ◽  
Gfp Chromophore ◽  
Partially Unfolded ◽  
Module Structures ◽  
Current Systems ◽  
Selection Of

Aptasensors became popular instruments in bioanalytical chemistry and molecular biology. To increase specificity, perspective signaling elements in aptasensors can be separated into a G-quadruplex (G4) part and a free fluorescent dye that lights up upon binding to the G4 part. However, current systems are limited by relatively low enhancement of fluorescence upon dye binding. Here, we added duplex modules to G4 structures, which supposedly cause the formation of a dye-binding cavity between two modules. Screening of multiple synthetic GFP chromophore analogues and variation of the duplex module resulted in the selection of dyes that light up after complex formation with two-module structures and their RNA analogues by up to 20 times compared to parent G4s. We demonstrated that the short duplex part in TBA25 is preferable for fluorescence light up in comparison to parent TBA15 molecule as well as TBA31 and TBA63 stabilized by longer duplexes. Duplex part of TBA25 may be partially unfolded and has reduced rigidity, which might facilitate optimal dye positioning in the joint between G4 and the duplex. We demonstrated dye enhancement after binding to modified TBA, LTR-III, and Tel23a G4 structures and propose that such architecture of short duplex-G4 signaling elements will enforce the development of improved aptasensors.

COMPOSITE MEASURE AS A METHOD OF THE IT SYSTEM FOR ORGANIC FARMING

2018 ◽  
Vol XX (5) ◽  
pp. 231-236
Author(s):  
Tomasz Woźniakowski
Keyword(s):  
Supply Chain ◽  
Organic Farming ◽  
The Internet ◽  
Composite Measure ◽  
Good Practices ◽  
Composite Measures ◽  
Comprehensive Information ◽  
Good Food ◽  
Selection Of

The paper describes the next stage of the project of an IT platform supporting farmers and organic producers. The article describes the implementation of the method developed in the course of research described in the previous, cited publication of the author. The method assumes the use of composite measures to create a universal gauge (“Good Food Print” ratio GFP) that provides comprehensive information on the good practices used throughout the supply chain of the final product of organic farming. The system offers consumers information in the formula of the Internet All Things, supports producers in the selection of the best suppliers and enables the maintenance of obligatory registers.

Aptamer-induced thermofluorimetric protein stabilization and G-quadruplex nucleic acid staining by SYPRO orange dye

2019 ◽  
Vol 43 (13) ◽  
pp. 4994-4997 ◽  
Author(s):  
Prashant S. Deore ◽  
Richard A. Manderville
Keyword(s):  
Nucleic Acid ◽  
Dissociation Constant ◽  
Melting Temperature ◽  
Binding Affinity ◽  
Thermal Stabilization ◽  
Protein Stabilization ◽  
Dna Aptamer ◽  
G Quadruplex

Serendipitously discovered nucleic acid staining by SYPRO Orange dye utilized to demonstrate proteins thermal stabilization (increase in melting temperature,Tm) as a function of increased DNA aptamer binding affinity (decrease in dissociation constant,Kd).

Sign in / Sign up

Export Citation Format

Share Document