scholarly journals BIOM-14. METHODS FOR SCREENING AND MONITORING BY GBM MASTER REGULATORY GENE MARKERS IN LIQUID BIOPSY

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii4-ii4
Author(s):  
Tianyi Liu ◽  
Son Le ◽  
David Tran
Keyword(s):  
Detection System ◽  
Low Cost ◽  
Regulatory Gene ◽  
False Negative ◽  
High Sensitivity ◽  
Normal Cells ◽  
Gene Markers ◽  
Master Regulators ◽  
Healthy Donors ◽  
Chromosomal Loci

Abstract BACKGROUND Current liquid-based cancer screening relies on massive deep NGS to detect rare cancer cell-derived genetic materials - a costly method fraught with high false-negative and false-positive rates. We aim to develop a non-NGS-centered, AI-directed liquid-based detection of GBM stem-like cells (GSC). METHODS Utilized a robust AI suite, NETZEN, we defined a common master regulatory gene network (MRGN) in GSC. Since master regulators (MR) in MRGN are developmentally restricted, their chromosomal loci are accessible in GSC but not in normal cells. Downstream factors in MRGN are massively overexpressed in GSC compared to normal cells. Thus, we measured 1) accessibility of MR genes using transposase/transposons carrying unique barcodes that can be detected after insertion into the MR’s predetermined accessible locations, and 2) expression of downstream factors using nested qRT-PCR, in PBMC from healthy controls spiked with known quantities of GSC or patients with GBM. RESULTS We characterized 10 MR genes in GSC with ≥1 GC-rich promoter region that is hypomethylated and accessible (ATACseq) in GBM/GSC per GSE70175/92460/52271 (19 samples) and GSE67633/96088 (14 samples), and hypermethylated and inaccessible in lymphocytes/PBMC per GSE98837 (6) and GSE74912 (13), respectively. Using barcoded transposons, we specifically disrupted 4 MR’s accessible regions only in GSC, not in PBMC. We also characterized 50 upregulated downstream factors with the top 20 having 3 to 5-orders-of-magnitude higher mean expression in GSC compared to PBMC (GSE79362/86884, 451 samples). Currently our method has a detection limit of 0.2–1 GSC in 106 PBMC. Using the first iteration, we detected GSC’s MRGN in blood samples of 14/14 GBM patients before resection, compared to in none of 15 healthy donors. CONCLUSIONS Chromosomal accessibility of MR and signal amplification in MRGN of GSC provide powerful substrates for a non-NGS, low-cost, liquid-based GBM detection system with potentially high sensitivity and specificity. Further testing and optimization are ongoing.

PATH-30. RAPID AND LOW-COST LIQUID BIOPSY-BASED METHOD FOR SCREENING AND MONITORING OF GBM BY MASTER REGULATORY GENE MARKERS IN GBM STEM-LIKE CELLS

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi121-vi121
Author(s):  
David Tran ◽  
Tianyi Liu ◽  
Son Le
Keyword(s):  
Detection System ◽  
Low Cost ◽  
Regulatory Gene ◽  
False Negative ◽  
High Sensitivity ◽  
Normal Cells ◽  
Gene Markers ◽  
Master Regulators ◽  
Healthy Donors ◽  
Chromosomal Loci

Abstract BACKGROUND Current liquid-based cancer screening relies on massive deep NGS to detect rare cancer-derived genetic materials - a costly method fraught with high false-negative and false-positive rates. We aim to develop a non-NGS-centered, AI-directed liquid-based detection of GBM stem-like cells (GSCs). METHODS Utilizing a robust AI suite, NETZEN, we defined a common master regulatory gene network (MRGN) of the GBM state in GSCs. Since master regulators (MRs) in MRGN are developmentally restricted, their chromosomal loci are accessible in GSCs but not in normal cells. Downstream factors in MRGN are massively overexpressed in GSCs compared to normal cells. Thus, we measured the following in PBMCs from healthy controls spiked with known quantities of GSCs and patients with GBM: 1) accessibility of MR genes using transposase/transposons carrying unique barcodes to be inserted into the MR’s predetermined accessible locations, and 2) expression of downstream factors using nested qRT-PCR. RESULTS We characterized 10 MRs in GSCs with ≥1 promoter region that is hypomethylated and accessible (by ATACseq) in GBM/GSCs per GSE70175-92460 (19 samples) and GSE67633-96088 (14 samples), or hypermethylated and inaccessible in lymphocytes/PBMCs per GSE98837 (6) and GSE74912 (13). Using barcoded transposons, we specifically disrupted 4 MR’s accessible foci only in GSCs, not in PBMCs. We also identified 50 downstream factors with the top 20 having 3 to 5-orders-of-magnitude higher mean expression in GSCs compared to PBMCs (GSE79362-86884, 451 samples). Currently our method has a detection limit of 0.2-1 GSC per 106 PBMCs. Using the first iteration, we detected MRGN of the GBM state in blood samples of 14 of 14 GBM patients before resection, compared to none of 15 healthy donors. CONCLUSIONS Chromosomal accessibility and signal amplification in MRGN of GSCs provide powerful substrates for a non-NGS, low-cost, liquid-based GBM detection system with high sensitivity and specificity. Further testing and optimization are ongoing.

scholarly journals Sliding wire detection system of electric lock screw tool based on motor characteristics

2021 ◽  
Vol 2137 (1) ◽  
pp. 012009
Author(s):  
Ning Zhang ◽  
Yinxin Yan ◽  
Houcheng Yang ◽  
Zhangsi Yu
Keyword(s):  
Real Time ◽  
Detection System ◽  
Low Cost ◽  
High Sensitivity ◽  
High Accuracy ◽  
Normal Operation ◽  
Automatic Assembly ◽  
The Real ◽  
Current Change ◽  
Real Time Detection

Abstract This paper presents a sliding wire detection system of electric screw locking tool based on the characteristics of motor. The system can judge whether the screw has sliding wire through the current change of motor during normal operation, and realize the real-time detection and alarm of sliding wire. The system has the advantages of high sensitivity, low cost and high accuracy. It can be widely used in automatic assembly and other fields.

Study on Green Gas Data Detection System

2013 ◽  
Vol 722 ◽  
pp. 322-327
Author(s):  
Feng Ying Zhang ◽  
Yun Hua Gao
Keyword(s):  
Indoor Air ◽  
Controller Design ◽  
Detection System ◽  
Low Cost ◽  
High Sensitivity ◽  
Data Detection ◽  
Wireless Data ◽  
Wireless Data Transmission ◽  
Pollution Detection ◽  
Treatment Analysis

This paper describes a wireless gas data detection system, based on CC1010 MCU as the core controller design measurement circuit of indoor air quality. IF in the air pollutants such as smokes and doors, TGS gas sensor sensitive to perceived pollutants transferred to the receiving device through the wireless function after CC1010 MCU treatment analysis. The system has the function of wireless data transmission, small size, high sensitivity, good stability, low cost, long life and other advantages of the peripheral circuits required little devices can be widely used in enclosed spaces such as indoor, automobile air pollution detection.

scholarly journals A MEMS-Based Multi-Parameter Integrated Chip and Its Portable System for Water Quality Detection

Micromachines ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 63 ◽  
Author(s):  
Ziyue Wu ◽  
Jiaqi Wang ◽  
Chao Bian ◽  
Jianhua Tong ◽  
Shanhong Xia
Keyword(s):  
Water Quality ◽  
Copper Ions ◽  
Detection System ◽  
Low Cost ◽  
Temperature Response ◽  
High Sensitivity ◽  
Integrated Sensor ◽  
Quality Detection ◽  
Portable Detection ◽  
Portable System

As an important means to protect water resources, water quality detection is of great social and economic significance. Water quality detection sensors processed by micro-electro-mechanical system (MEMS) technology have the advantages of low-cost, small size, and high sensitivity. In this paper, a multi-parameter water quality detection integrated sensor chip is further studied, and a portable detection system using this chip is developed. Temperature, pH, oxidation-reduction potential (ORP), conductivity and concentration of copper ions (Cu2+) are selected as typical water quality parameters. Experiments of sensor calibrations using this portable detection system were performed in standard solutions. The sensor has a sensitivity of −57.34 mV/pH in pH detection and 5.95 Ω/°C in temperature response. ORP is directly detected by Pt microelectrode on the chip and the relative error is less than 3%. The electrode constant of the sensor is 1.416 cm−1 and the linearity is 0.9995 in conductivity detection. With the gold nanoparticles deposited on the electrode, the detection peak of Cu2+ appears at 280 mV and the sensor shows good linearity to the concentration of Cu2+ in the range of 0–0.6 mg/L. The detection limit of Cu2+ concentration is 2.33 μg/L. Through measurement and calculation, the accuracy of the portable system is within 4%. This portable multi-parameter water quality detection system with the MEMS-based integrated chip shows great potential in the field and fast detection.

Role and limits of testicular phleboscintigraphy in the diagnostic evaluation of varicocele

1994 ◽  
Vol 61 (2) ◽  
pp. 98-102
Author(s):  
A. Raimoldi ◽  
G.L. Berti ◽  
V. Giola ◽  
G.L. Leidi ◽  
A. Maccaroni ◽  
...  
Keyword(s):  
Doppler Ultrasonography ◽  
Low Cost ◽  
False Negative ◽  
High Sensitivity ◽  
Diagnostic Evaluation ◽  
Ultrasound Technique ◽  
Negative Results ◽  
False Negative Results ◽  
First Choice ◽  
Choice Method

The Authors wanted to check the reliability of testicular phleboscintigraphy in the diagnostic evaluation of varicocele, comparing it to Doppler ultrasonography. To this end, 98 patients affected by clinically evident left idiopathic varicocele were tested. The two methods gave equivalent results in all patients affected by 2nd and 3rd clinical degree varicocele, while testicular phleboscintigraphy gave false negative results in 7 patients with 1st degree varicocele, due to renospermatic retrograde flow. Therefore, in the Authors' opinion, the Doppler ultrasound technique can be considered as a first choice method in the study of varicocele, thanks to its high sensitivity associated with low cost, whereas testicular phleboscintigraphy can be used either when Doppler ultrasonography evaluation is not reliable or as a second examination to confirm the Doppler results, especially in patients with 2nd and 3rd clinical degree varicocele.

scholarly journals Low-Cost Benzene Toluene Xylene Measurement Gas System Based on the Mini Chromatographic Cartridge

Sensors ◽  
2020 ◽  
Vol 21 (1) ◽  
pp. 125
Author(s):  
Emiliano Zampetti ◽  
Paolo Papa ◽  
Joshua Avossa ◽  
Andrea Bearzotti ◽  
Catia Balducci ◽  
...  
Keyword(s):  
Detection System ◽  
Low Cost ◽  
Control Unit ◽  
High Sensitivity ◽  
Reliable Technique ◽  
Qualified Personnel ◽  
Gas System ◽  
Benzene Toluene ◽  
Photo Ionization ◽  
Mini Pump

Benzene, toluene and xylene (BTX) are an important part of the volatile organic compounds (VOCs) to be detected and monitored in the air, due to their toxicity towards human health. One of the most reliable technique used in BTX detection is gas chromatography (GC), which presents a high sensitivity. On the other hand, it has important drawbacks, such as high costs, the need for qualified personnel and frequent maintenance. To overcome these drawbacks, this work reports the development of a low cost and portable BTX gas detection system based on a mini chromatographic cartridge, a photo ionization detector (PID), a simple control unit (based on Arduino architecture) and a mini pump. In order to separate the BTX components, we propose the use of a cartridge 80 mm in length, composed of several commercial chromatographic column sections. To test the system performances, we have injected different amounts (from about 0.3 to 5.3 µg) of benzene, toluene and xylene and two of the most frequent possible interferents (ethanol, acetone). Experimental results have shown different retention time values (i.e., 25 ± 0.5 s, 51 ± 1.2 s and 117 ± 4 s, respectively) for benzene, toluene and xylene.

Smartphone-based technology for nanomolecular detection of aflatoxin B1 by aptamer-conjugated magnetic nanoparticles

2021 ◽  
pp. 1-12
Author(s):  
A. Rafati ◽  
N. Dorosti ◽  
P. Gill
Keyword(s):  
Magnetic Nanoparticles ◽  
Detection System ◽  
Colorimetric Detection ◽  
False Negative ◽  
Enzymatic Reaction ◽  
High Sensitivity ◽  
Rice Flour ◽  
Food Contaminants ◽  
Hybrid Nanomaterial ◽  
Colorimetric Measurement

The need for a healthy market in the rapid and accurate screening of a variety of pathogenic agents and toxins in the environment and food has led to an increase in the development of new biosensors, which have ideal characteristics, such as high sensitivity and specificity with rapid detection and simple preparation of the sample. Among the food contaminants, mycotoxins have been identified as a major challenge for the food industry, and rapid and accurate detection has attracted the attention of food inspection and monitoring organisations. In this study, a nanomolecular detection method is described using aflatoxin B1 (AFB1)-specific aptamers attached to streptavidin-coated magnetic nanoparticles. A prominent feature of the AFB1-specific aptamers is a guanine-rich (G-rich) sequence with a G-quadruplex structure after capturing AFB1 molecules and mimicking peroxidase activity. The enzymatic reaction evaluated in the presence of chromogenic substrate and measurement is done by a smartphone-specific application for colorimetric measurement. The results indicated that the assay could measure AFB1 in rice, flour, seed, maize, and pistachio. In addition, the application of hybrid nanomaterial technology resulting from the binding of biotin-labelled aptamers to the surface of streptavidin-coated magnetic nanoparticles minimises preparation and treatment of samples, improves results, and consequently reduces false negative and positive responses in the detection field. This study may eventually lead to the design and development of a fast, sensitive, specific, and on-site AFB1-based nanomolecular colorimetric detection system via a smartphone-based application that can be readily accessible to all applicants, from professionals to manufacturers of foodstuffs.

WeMos IoT Controller-Based Low-Cost Fall Detection System for Elderly Users

2021 ◽  
Vol 50 ◽  
pp. 59-72
Author(s):  
Mohammed Faeik Ruzaij Al-Okby ◽  
Kerstin Thurow
Keyword(s):  
False Positive ◽  
Detection System ◽  
Low Cost ◽  
False Negative ◽  
Lithium Ion ◽  
Fall Detection ◽  
The Elderly ◽  
The Body ◽  
Detection Systems ◽  
Alarm Message

Fall detection systems for the elderly are very important to protect this type of users. The early detection of the fall of the elderly has a major impact on saving their lives and avoiding the deterioration of the negative medical effects resulting from the effect of the patient falling on a hard surface. One of the constraints in fall detection systems are false-negative errors (no fall detection) or false-positive errors (sending a false warning without real fall accident). These errors have to be reduced significantly. In this paper, an innovative method to reduce fall detection system errors is proposed. The system consists of two orientation detection sensors to track the body orientation instead of using a single sensor in the previous systems which enhances the system accuracy and reduces the false-negative and false-positive errors. The system uses a small size IoT-based controller to process the sensor's information and make the alarm decision based on specific thresholds. The output alarm of the system includes an email sent to the caregivers via the embedded Wi-Fi ESP8266 module as well as an SMS message to the caregivers’ phones via GSM modules to ensure that the alarm message arrives in the absence of internet coverage for the patient or the caregiver. The system is powered by a small lithium-Ion battery. All sensors and modules of the system are combined in a small rubber box that can be fixed in a waist belt or the chest rejoin of the user body. Several tests have been made in different procedures. The tests revealed that the new approach improves the accuracy of the system and reduces the possibility of triggering wrong alarms.

Design on the Amplifier Circuit of Metal-Oxide Semiconductor Gas-Sensitive Sensor

2012 ◽  
Vol 220-223 ◽  
pp. 1939-1942 ◽  
Author(s):  
Xi Feng Liang ◽  
Li Hao Liu
Keyword(s):  
Metal Oxide ◽  
Detection System ◽  
Low Cost ◽  
High Sensitivity ◽  
Metal Oxide Semiconductor ◽  
Oxide Semiconductor ◽  
Gas Detection ◽  
Amplifier Circuit ◽  
Detection Systems ◽  
System A

Metal-oxide semiconductor gas-sensitive sensors have various advantages as the basic devices of gas detection systems, such as high sensitivity, fast responsibility and low cost, etc. They are widely applied to many fields. Amplifier circuit is an important section of gas detection system. A new type of amplifier circuit including a three-stage operational amplifier was designed in the paper which can effectively eliminate the influence of the follow-up circuit on the sensor output. Theory analysis and experimental simulations were performed. The results show that the output voltage signals have a linear relation with the concentrations of the detected gas.

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