scholarly journals Efficient modulation of photosynthetic apparatus confers desiccation tolerance in the resurrection plant Boea hygrometrica

2017 ◽  
Vol 58 (11) ◽  
pp. 1976-1990 ◽  
Author(s):  
Tinghong Tan ◽  
Yanni Sun ◽  
Shishuai Luo ◽  
Chao Zhang ◽  
Huapeng Zhou ◽  
...  
Keyword(s):  
Desiccation Tolerance ◽  
Photosynthetic Apparatus ◽  
Resurrection Plant ◽  
Boea Hygrometrica

scholarly journals DNA methylation-mediated modulation of rapid desiccation tolerance acquisition and dehydration stress memory in the resurrection plant Boea hygrometrica

PLoS Genetics ◽  
2021 ◽  
Vol 17 (4) ◽  
pp. e1009549
Author(s):  
Run-Ze Sun ◽  
Jie Liu ◽  
Yuan-Yuan Wang ◽  
Xin Deng
Keyword(s):  
Dna Methylation ◽  
Desiccation Tolerance ◽  
Protein Modification ◽  
Dehydration Stress ◽  
Resurrection Plant ◽  
Gene Body Methylation ◽  
Dehydration Tolerance ◽  
Stress Memory ◽  
Drought Acclimation ◽  
Boea Hygrometrica

Pre-exposure of plants to various abiotic conditions confers improved tolerance to subsequent stress. Mild drought acclimation induces acquired rapid desiccation tolerance (RDT) in the resurrection plant Boea hygrometrica, but the mechanisms underlying the priming and memory processes remain unclear. In this study, we demonstrated that drought acclimation-induced RDT can be maintained for at least four weeks but was completely erased after 18 weeks based on a combination of the phenotypic and physiological parameters. Global transcriptome analysis identified several RDT-specific rapid dehydration-responsive genes related to cytokinin and phospholipid biosynthesis, nitrogen and carbon metabolism, and epidermal morphogenesis, most of which were pre-induced by drought acclimation. Comparison of whole-genome DNA methylation revealed dehydration stress-responsive hypomethylation in the CG, CHG, and CHH contexts and acclimation-induced hypermethylation in the CHH context of the B. hygrometrica genome, consistent with the transcriptional changes in methylation pathway genes. As expected, the global promoter and gene body methylation levels were negatively correlated with gene expression levels in both acclimated and dehydrated plants but showed no association with transcriptional divergence during the procedure. Nevertheless, the promoter methylation variations in the CG and CHG contexts were significantly associated with the differential expression of genes required for fundamental genetic processes of DNA conformation, RNA splicing, translation, and post-translational protein modification during acclimation, growth, and rapid dehydration stress response. It was also associated with the dehydration stress-induced upregulation of memory genes, including pre-mRNA-splicing factor 38A, vacuolar amino acid transporter 1-like, and UDP-sugar pyrophosphorylase, which may contribute directly or indirectly to the improvement of dehydration tolerance in B. hygrometrica plants. Altogether, our findings demonstrate the potential implications of DNA methylation in dehydration stress memory and, therefore, provide a molecular basis for enhanced dehydration tolerance in plants induced by drought acclimation.

Desiccation-tolerance specific gene expression in leaf tissue of the resurrection plant Sporobolus stapfianus

2007 ◽  
Vol 34 (7) ◽  
pp. 589 ◽  
Author(s):  
Tuan Ngoc Le ◽  
Cecilia K. Blomstedt ◽  
Jianbo Kuang ◽  
Jennifer Tenlen ◽  
Donald F. Gaff ◽  
...  
Keyword(s):  
Drought Stress ◽  
Desiccation Tolerance ◽  
Molecular Mechanisms ◽  
Leaf Tissue ◽  
Resurrection Plant ◽  
Specific Gene ◽  
Irreversible Damage ◽  
Cellular Processes ◽  
Sporobolus Stapfianus ◽  
Normal Metabolism

The desiccation tolerant grass Sporobolus stapfianus Gandoger can modulate cellular processes to prevent the imposition of irreversible damage to cellular components by water deficit. The cellular processes conferring this ability are rapidly attenuated by increased water availability. This resurrection plant can quickly restore normal metabolism. Even after loss of more than 95% of its total water content, full rehydration and growth resumption can occur within 24 h. To study the molecular mechanisms of desiccation tolerance in S. stapfianus, a cDNA library constructed from dehydration-stressed leaf tissue, was differentially screened in a manner designed to identify genes with an adaptive role in desiccation tolerance. Further characterisation of four of the genes isolated revealed they are strongly up-regulated by severe dehydration stress and only in desiccation-tolerant tissue, with three of these genes not being expressed at detectable levels in hydrated or dehydrating desiccation-sensitive tissue. The nature of the putative proteins encoded by these genes are suggestive of molecular processes associated with protecting the plant against damage caused by desiccation and include a novel LEA-like protein, and a pore-like protein that may play an important role in peroxisome function during drought stress. A third gene product has similarity to a nuclear-localised protein implicated in chromatin remodelling. In addition, a UDPglucose glucosyltransferase gene has been identified that may play a role in controlling the bioactivity of plant hormones or secondary metabolites during drought stress.

Molecular cloning and differential expression of sHSP gene family members from the resurrection plant Boea hygrometrica in response to abiotic stresses

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Zhennan Zhang ◽  
Bo Wang ◽  
Dongmei Sun ◽  
Xin Deng
Keyword(s):  
Molecular Chaperones ◽  
Abiotic Stresses ◽  
Potential Role ◽  
Resurrection Plant ◽  
High Calcium ◽  
Shsp Gene ◽  
Alkaline Conditions ◽  
Boea Hygrometrica ◽  
Shock Proteins

AbstractSmall heat shock proteins (sHSPs) are a class of molecular chaperones that bind to and prevent aggregation of proteins. To assess the potential role of sHSPs in protection against abiotic stresses, we conducted a screening of sHSP genes from the desiccation-tolerant resurrection plant Boea hygrometrica, which is widespread in East Asia in alkaline, calcium-rich limestone crevices. In total, 25 sHSP genes, belonging to six subgroups, were identified from the desiccated leaves of B. hygrometrica. Ten of these genes were cloned and named according to the nomenclature proposed for sHSPs. Transcripts of all these BhsHSPs were detectable in fresh leaves, but only 6 genes were induced after desiccation, and remained high during rehydration. Four of the cytosol-targeted BhsHSP genes were up-regulated under treatments, such as heat, cold, alkaline conditions, high calcium, oxidation, or application of the phytohormone abscisic acid. Together, these results demonstrate that CI and CII sHSPs, especially Bh17.9CI and Bh17.4BCII, are associated with abiotic stresses, and may function in the maintenance of protein stability, aiding in the adaptations to extreme environmental conditions in which B. hygrometrica can survive.

scholarly journals Water molecular structure underpins extreme desiccation tolerance of the resurrection plant Haberlea rhodopensis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shinichiro Kuroki ◽  
Roumiana Tsenkova ◽  
Daniela Moyankova ◽  
Jelena Muncan ◽  
Hiroyuki Morita ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Desiccation Tolerance ◽  
Resurrection Plant ◽  
Haberlea Rhodopensis

scholarly journals A molecular physiological review of vegetative desiccation tolerance in the resurrection plant Xerophyta viscosa (Baker)

Planta ◽  
2015 ◽  
Vol 242 (2) ◽  
pp. 407-426 ◽  
Author(s):  
Jill M. Farrant ◽  
Keren Cooper ◽  
Amelia Hilgart ◽  
Kamal O. Abdalla ◽  
Joanne Bentley ◽  
...  
Keyword(s):  
Desiccation Tolerance ◽  
Resurrection Plant

scholarly journals A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant Xerophyta schlechteri during Dehydration

Life ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1242
Author(s):  
Hawwa Gabier ◽  
David L. Tabb ◽  
Jill M. Farrant ◽  
Mohamed Suhail Rafudeen
Keyword(s):  
Desiccation Tolerance ◽  
Early Response ◽  
Resurrection Plant ◽  
Late Response ◽  
Biological Processes ◽  
Label Free ◽  
Relative Water ◽  
Leaf Tissues ◽  
Insight Into ◽  
Selection Of

Vegetative desiccation tolerance, or the ability to survive the loss of ~95% relative water content (RWC), is rare in angiosperms, with these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, with its understanding requiring a comprehensive systems biology approach. The aim of the current study was to conduct a label-free proteomic analysis of leaves of the resurrection plant Xerophyta schlechteri in response to desiccation. A targeted metabolomics approach was validated and correlated to the proteomics, contributing the missing link in studies on this species. Three physiological stages were identified: an early response to drying, during which the leaf tissues declined from full turgor to a RWC of ~70–80%, a mid-response in which the RWC declined to 40% and a late response where the tissues declined to 10% RWC. We identified 517 distinct proteins that were differentially expressed, of which 253 proteins were upregulated and 264 were downregulated in response to the three drying stages. Metabolomics analyses, which included monitoring the levels of a selection of phytohormones, amino acids, sugars, sugar alcohols, fatty acids and organic acids in response to dehydration, correlated with some of the proteomic differences, giving insight into the biological processes apparently involved in desiccation tolerance in this species.

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