Tung Tree (Vernicia fordii, Hemsl.) Genome and Transcriptome Sequencing Reveals Co-Ordinate Up-Regulation of Fatty Acid β-Oxidation and Triacylglycerol Biosynthesis Pathways During Eleostearic Acid Accumulation in Seeds

2018 ◽  
Vol 59 (10) ◽  
pp. 1990-2003 ◽  
Author(s):  
Peng Cui ◽  
Qiang Lin ◽  
Dongming Fang ◽  
Lingling Zhang ◽  
Rongjun Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Transcriptome Sequencing ◽  
Eleostearic Acid ◽  
Tung Tree ◽  
Vernicia Fordii ◽  
Triacylglycerol Biosynthesis ◽  
Acid Accumulation

scholarly journals Molecular cloning and expression profile of β-ketoacyl-acp synthase gene from tung tree (Vernicia fordii Hemsl.)

Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 143-159 ◽  
Author(s):  
Hongxu Long ◽  
Xiaofeng Tan ◽  
Fangfang Yan ◽  
Lin Zhang ◽  
Ze Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Full Length ◽  
Cloning And Expression ◽  
Tung Tree ◽  
Degenerate Primers ◽  
Expression Levels ◽  
Full Length Cdna ◽  
Vernicia Fordii

Tung tree (Vernicia fordii) is an important woody oil tree. Tung tree seeds contain 50-60% oil with approximately 80 mole ?-eleostearic acid (9 cis, 11 trans, 13 trans octadecatrienoic acid). Fatty acid synthesis is catalyzed by the concerted action of acetyl-CoA carboxylase and fatty acid synthase, a multienzyme complex including ?-ketoacyl-acyl-carrier-protein synthase (KAS). Little is known about KAS in tung tree. The objective of this study was to clone KAS genes and analyze their expression profiles in tung tree. A full-length cDNA encoding KAS III and a partial cDNA encoding KAS II were isolated from tung tree by PCR cloning using degenerate primers and rapid amplification of cDNA ends system. The full-length cDNA of VfKAS III was 1881 bp in length with an open reading frame of 1212 bp. VfKAS III genomic DNA was also isolated and sequenced, which contained 8 exons in 5403 bp length. The deduced VfKAS III protein shared approximately 80% identity with homologous KAS IIIs from other plants. Quantitative PCR analysis revealed that KAS II and KAS III were expressed in all of the tissues and organs tested but exhibited different expression patterns in tung tree. The expression levels of KAS II in young tissues were much lower than those in mature tissues, whereas the highest expression levels of KAS III were observed in young stem and young leaf. These results should facilitate further studies on the regulation of tung oil biosynthesis by KAS in tung tree.

Fatty acid accumulation analysis based on transcriptome sequencing of developmental herbaceous peony ‘Hangshao’ ( Paeonia lactiflora ‘Hangshao’) seeds

2019 ◽  
Author(s):  
Jiasong Meng ◽  
Yuhan Tang ◽  
Jing Sun ◽  
Jun Tao
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Transcriptome Sequencing ◽  
Fatty Acid Biosynthesis ◽  
Fatty Acid Elongation ◽  
Paeonia Lactiflora ◽  
Acid Biosynthesis ◽  
Herbaceous Peony ◽  
Fatty Acid Accumulation ◽  
Acid Accumulation

Abstract Background: Paeonia lactiflora ‘Hangshao’ is widely cultivated in China because its root can be used to produce raw materials for traditional Chinese medicine ‘Radix Paeoniae Alba’. Due to the presence of abundant unsaturated fatty acids in its seed, it also can be regarded as a new oil plant. However, the process of the biosynthesis of unsaturated fatty acid in herbaceous peony ‘Hangshao’ remained largely unknown. Therefore, transcriptome analysis is helpful to better understand the molecular mechanisms. Results: Five main fatty acids, stearic acid, palmitic acid, oleic acid, linoleic acid and α-linolenic acid, were detected, and their absolute contents increased first and then decreased during seed development. A total of 150,156 Unigenes were obtained by transcriptome sequencing, with an average length of 1,030 bp. There were 1,550 Unigenes annotated in the seven functional databases including NR, NT, GO, KOG, KEGG, SwissProt and InterPro. Based on KEGG database, 1,766 Unigenes were annotated in the lipid metabolic pathways, among which 103, 74 and 70 Unigenes are annotated into fatty acid biosynthesis pathway, fatty acid elongation pathway and unsaturated fatty acid synthesis pathway; respectively. A total of 1480 DEGs were detected. Among them, 83 DEGs were enriched in the fatty acid metabolism pathway, including 12 DEGs involved in the fatty acid biosynthesis and 1 DEG involved in fatty acid elongation. Furthermore, qRT-PCR was used to analyze the expression patterns of nine fatty acid biosynthetic related genes including FBCP, BC, FabD, FabF, FATB, KCR, FAD2, FAD3 and FAD7, and it showed that they all highest expressed at 45 DAF. Conclusions: This study provides the first comprehensive genomic resources characterizing herbaceous peony seeds gene expression at the transcriptional level. These data lay the foundation for elucidating the molecular mechanism of the lipid biosynthesis and fatty acid accumulation for herbaceous peony 'Hangshao'.

scholarly journals FAD2-DGAT2Genes Coexpressed in EndophyticAspergillus fumigatusDerived from Tung Oilseeds

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Yi-Cun Chen ◽  
Yang-Dong Wang ◽  
Qin-Qin Cui ◽  
Zhi-Yong Zhan
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Fatty Acid Desaturase ◽  
Fold Increase ◽  
Great Promise ◽  
Tung Tree ◽  
Genetically Engineer ◽  
Acid Accumulation ◽  
Fatty Acid Desaturase 2

Recent efforts to genetically engineer plants that contain fatty acid desaturases to produce valuable fatty acids have made only modest progress. Diacylglycerol acyltransferase 2 (DGAT2), which catalyzes the final step in triacylglycerol (TAG) assembly, might potentially regulate the biosynthesis of desired fatty acids in TAGs. To study the effects of tung tree (Vernicia fordii)vfDGAT2in channeling the desired fatty acids into TAG,vfDGAT2combined with the tung tree fatty acid desaturase-2 (vfFAD2) gene was co-introduced intoAspergillus fumigatus, an endophytic fungus isolated from healthy tung oilseed. Two transformants coexpressingvfFAD2andvfDGAT2showed a more than 6-fold increase in linoleic acid production compared to the originalA. fumigatusstrain, while a nearly 2-fold increase was found in the transformant expressing onlyvfFAD2. Our data suggest thatvfDGAT2plays a pivotal role in promoting linoleic acid accumulation in TAGs. This holds great promise for further genetic engineering aimed at producing valuable fatty acids.

scholarly journals Fatty Acid Profile and Unigene-Derived Simple Sequence Repeat Markers in Tung Tree (Vernicia fordii)

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105298 ◽  
Author(s):  
Lin Zhang ◽  
Baoguang Jia ◽  
Xiaofeng Tan ◽  
Chandra S. Thammina ◽  
Hongxu Long ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Simple Sequence Repeat ◽  
Tung Tree ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Vernicia Fordii ◽  
Simple Sequence

IDENTIFICATION OF CONJUGATED TRIENE FATTY ACIDS IN CERTAIN SEED OILS

1962 ◽  
Vol 40 (11) ◽  
pp. 2078-2082 ◽  
Author(s):  
C. Y. Hopkins ◽  
Mary J. Chisholm
Keyword(s):  
Fatty Acid ◽  
Eleostearic Acid ◽  
Octadecatrienoic Acid ◽  
Seed Oils ◽  
Punicic Acid ◽  
Calendula Officinalis ◽  
Conjugated Fatty Acid ◽  
Mild Conditions ◽  
Conjugated Triene ◽  
Major Acid

Seed oils were hydrolyzed under mild conditions and the major conjugated fatty acid of each oil was isolated and identified. In two families, species which were closely related botanically contained different but isomeric acids. Thus, in the Bignoniaceae, Jacaranda chelonia had cis trans,cis-8,10,12-octadecatrienoic acid as a major acid while Catalpa speciosa had trans,trans,cis-9,11,13-octadecatrienoic acid. In the Cucurbitaceae, Momordica charantia had the ordinary cis,trans,trans-9,11,13-octadecatrienoic (α-eleostearic) acid while M. balsamina had cis,trans,cis-9,11,13-octadecatrienoic (punicic) acid. M. balsamina is a new and convenient source of punicic acid. α-Eleostearic acid was identified as a major acid in examples of Valerianaceae and Rosaceae. Further proof was obtained that the fatty acid of Calendula officinalis (Compositae) is trans,trans,cis-8,10,12-octadecatrienoic acid.

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