Characterization of Two Growth Period QTLs Reveals Modification of PRR3 Genes During Soybean Domestication

In response to the need for developing a better model to predict frost formation and defrosting processes in freezer and evaporator applications, a microscopic analysis of frost growth on a flat surface is conducted to determine the microscopic characteristics of a frost layer during the early growth period when sub-cooled droplets are formed and changed to the ice. The surface characterization is performed by employing the holographic interferometry technique to determine the air-frost interface temperature, and the video microscope to determine the mean droplet size and ice particle fractions. Typical experimental results are presented to demonstrate the test technique. Preliminary experimentally determined frost thickness and air-frost interface temperature are compared with simulation results.

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MORPHOPHYSIOLOGY OF RHIZOBIA ISOLATED FROM NATIVE FORAGE OF FABACEAE OF THE CAATINGA

Revista Caatinga ◽

10.1590/1983-21252019v32n406rc ◽

2019 ◽

Vol 32 (4) ◽

pp. 904-914

Author(s):

SUELANE DE MELO DIAS ◽

ALBERÍCIO PEREIRA DE ANDRADE ◽

ADAILSON PEREIRA DE SOUZA ◽

ANDRÉ LUIZ RODRIGUES MAGALHÃES ◽

RISELANE DE LUCENA ALCÂNTARA BRUNO ◽

...

Keyword(s):

High Temperatures ◽

Native Species ◽

Growth Period ◽

Semiarid Region ◽

Ph Tolerance ◽

Resistance To Stress ◽

Macroptilium Atropurpureum ◽

Physiological Tests ◽

Exopolysaccharides Production

ABSTRACT The rhizobia found in Fabaceae plants of the Caatinga may have potential as fodder in the semiarid region, thus increasing the availability of feed for animal production in this region. This study aimed to characterize the morphophysiology of rhizobia strains of the forage species Mimosa tenuiflora (Jurema preta), Macroptilium atropurpureum (Siratro), and Desmanthus pernambucanus (Jureminha). The soil used as the substrate was collected at specific locations in the municipalities of Sertânia, Arcoverde, and São Bento do Una, state of Pernambuco. The characterization of rhizobial colonies included growth period, diameter, elevation, transparency, exopolysaccharides production, and color. In addition, salinity, temperature, and pH tolerance tests of the native rhizobia were conducted. Evaluation of cultures showed that the isolates formed colonies with flat elevation, moderate production of exopolysaccharides, coloration varying from cream to white, and a smooth surface. Physiological tests of abiotic stress resistance showed that the native species isolated from M. tenuiflora were more resistant when grown in soils from the municipality of Sertânia and isolates from M. atropurpureum and D. pernambucanus had high resistance to high temperatures, regardless of place of farming. It can be concluded that native rhizobia from the Semiarid region of Pernambuco show resistance to stress caused by high temperatures, salinity variation, and pH. These characteristics are influenced by soil and the botanical species in symbiosis with the rhizobia.

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Valepotriate content in different in vitro cultures of Valerianaceae and characterization of Valeriana officinalis L. callus during a growth period

Pharmaceutisch Weekblad ◽

10.1007/bf02332944 ◽

1983 ◽

Vol 5 (5) ◽

pp. 205-209 ◽

Cited By ~ 6

Author(s):

C. Violon ◽

N. van Cauwenbergh ◽

A. Vercruysse

Keyword(s):

Growth Period ◽

In Vitro Cultures ◽

Valeriana Officinalis

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Development of new microsatellite markers for DNA fingerprinting pattern of black gram (Vigna mungo L. Hepper) and green gram (Vigna radiate L. Wilckzek)

Genetika ◽

10.2298/gensr2003161p ◽

2020 ◽

Vol 52 (3) ◽

pp. 1161-1179

Author(s):

Kaushik Panigrahi ◽

Puranjaya Panigrahi ◽

Ayesha Mohanty ◽

Purandar Mandal ◽

Basudeba Satapathy

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Banding Pattern ◽

Growth Period ◽

Green Gram ◽

Black Gram ◽

Crop Species ◽

Genetic Studies ◽

Ssr Primers

SSR markers are considered to be the most ideal marker for genetic studies because they are multi-allelic, abundant, randomly and widely distributed throughout the genome, co-dominant that could differentiate plants with homozygous or heterozygous alleles, simple to assay, highly reliable, reproducible. Microsatellite markers are highly polymorphic and informative and could be successfully used for genome analysis in black gram & green gram. Microsatellite markers were used to evaluate genetic diversity in 17 indigenous cultivars of pulse crops (11 cultivars of green gram and 6 cultivars of black gram respectively). They are subjected to variability analysis with 26 microsatellite markers for identification efficient primers to conclude the nature of molecular diversity present among the pulses. The SSR primer G228 showed 63.63% of polymorphism followed by MB-SSR 238 (45.45%) and G006 (36.36%). The 12 microsatellite markers produced 15.90 % polymorphism with banding ranged up to 7 with an average of 2.3 polymorphic banding patterns per SSR primer. Similarly for black gram, three random microsatellite primers G006 (50%) and G166 and G204 (33.33%) revealed considerable DNA polymorphism. The 14 random SSR primers produced 8.33% of polymorphism with banding ranged up to three with an average of 1.28 polymorphic banding pattern per SSR primer. The Distinguish Power (D), Polymorphism Information Content (PIC) value and Marker Index (MI) values revealed some SSR primers like G006, G204 and G166 can alone amplified distinct banding pattern, where as a combination of (G228+G006), (G228+G304) for green gram and the combination (G006+G166) can be used for black gram for ascertaining genetic diversity at any stage of crop growth period for green gram or black gram. From the present study we can conclude that selective microsatellite markers are highly polymorphic, informative and easily reproducible, which can be successfully used either as single or with combination for molecular characterization of crop species belonging to Vigna species.

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Inhibitory Effect of 2′-Substituted Nucleosides on Hepatitis C Virus Replication Correlates with Metabolic Properties in Replicon Cells

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.49.5.2050-2058.2005 ◽

2005 ◽

Vol 49 (5) ◽

pp. 2050-2058 ◽

Cited By ~ 35

Author(s):

Joanne E. Tomassini ◽

Krista Getty ◽

Mark W. Stahlhut ◽

Sung Shim ◽

Balkrishen Bhat ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Cell Lines ◽

Growth Period ◽

Inhibitory Effect ◽

Chemotherapeutic Agents ◽

Metabolic Properties ◽

Ns5b Polymerase

ABSTRACT Nucleosides have been widely used in the treatment of viral diseases, but relatively few have been identified as inhibitors of hepatitis C virus (HCV). The modified ribonucleosides, 2′-C-methyl-adenosine and 2′-O-methyl-cytidine, are potent inhibitors of HCV replication which specifically target the NS5B polymerase. Herein, a more extensive characterization of the effect of these compounds upon HCV replication in subgenomic replicons is reported. A highly selective antireplicative effect induced by the nucleosides in replicon-containing cell lines was maintained during an exponential growth period with potencies which paralleled the reduction of both positive- and negative-strand RNA replication. Moreover, the inhibitory effect closely correlated with the intrinsic metabolic properties of differing replicon clonal lines. Interestingly, while 2′-C-methyl-adenosine elicited similar inhibitory potencies in different cell lines, 2′-O-methyl-cytidine was found to be inactive in one replicon cell line tested, although the corresponding triphosphates comparably inhibited the in vitro activity of replication complexes isolated from these cells and the activity of NS5B polymerase using synthetic templates. The lack of antireplicative effect, attributed to poor intracellular conversion of the 2′-O-methyl-cytidine nucleoside to the active 5′-triphosphate, was reversed using a monophosphate prodrug. Thus, although replicon cells are useful for evaluating the effect of inhibitors upon HCV replication, these findings have important implications for their use in the identification and characterization of nucleosides and other chemotherapeutic agents requiring cellular metabolism.

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Biochemical and Molecular Characterization of the Rice Chalcone Isomerase Family

Plants ◽

10.3390/plants10102064 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2064

Author(s):

Sang-Il Park ◽

Hye-Lin Park ◽

Seong-Hee Bhoo ◽

Sang-Won Lee ◽

Man-Ho Cho

Keyword(s):

Catalytic Properties ◽

Ultra Violet ◽

Growth Period ◽

Chalcone Isomerase ◽

The Other ◽

Type I ◽

Activity Assay ◽

Key Enzyme ◽

Bona Fide

Chalcone isomerase (CHI) is a key enzyme in flavonoid biosynthesis. In plants, CHIs occur in multigene families, and they are divided into four types, types I–IV. Type I and II CHIs are bona fide CHIs with CHI activity, and type III and IV CHIs are non-catalytic members with different functions. Rice contains seven CHI family genes (OsCHIs). Molecular analysis suggested that OsCHI3 is a type I CHI, and the other OsCHIs were classified into types III and IV. To elucidate their biochemical functions, OsCHI1, OsCHI3, OsCHI6, and OsCHI7 were expressed in Escherichia coli, and the recombinant OsCHI proteins were purified. An activity assay of recombinant OsCHIs showed that OsCHI3 catalyzed the isomerization of naringenin chalcone and isoliquiritigenin, whereas the other recombinant OsCHIs had no CHI activity. OsCHI3 also exhibited a strong preference to naringenin chalcone compared to isoliquiritigenin, which agrees well with the catalytic properties of type I CHIs. These results ascertain OsCHI3 to be a bona fide CHI in rice. OsCHI3 and the other OsCHIs were expressed constitutively throughout the rice growth period and different tissues. OsCHI3 expression was induced immediately in response to ultra-violet (UV) stress, suggesting its involvement in the biosynthesis of sakuranetin, a flavonoid phytoalexin in rice.

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Characterization of Angiogenesis during Skin Wound Repair by Porous Silk Fibroin Film

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.175-176.181 ◽

2011 ◽

Vol 175-176 ◽

pp. 181-185 ◽

Cited By ~ 2

Author(s):

Kui Hua Zhan ◽

Lun Bai ◽

Guo Ping Guan ◽

Hong Qin Dai

Keyword(s):

Silk Fibroin ◽

Rapid Growth ◽

Wound Repair ◽

Growth Period ◽

Interface Layer ◽

Skin Wound ◽

Material Layer ◽

Silk Fibroin Film ◽

Rehabilitation Period

Based on our experimental data of neovascularization of porous silk fibroin films (PSFF)，in this study we characterized the formation of new blood vessel system at different periods. Firstly, we regarded a wound as two sections, namely, an interface layer and a material layer. Angiogenesis of the interface layer underwent three periods that were rehabilitation period of primary vascular plexus (postoperative 1st day-3rd day), rapid growth period (postoperative 3rd day - 7th day) and remodeling period (after the 7th day). In addition, angiogenesis of the material layer underwent initiation period (postoperative 5th day - 7th day), rapid growth period (postoperative 7th day - 13th day) and remodeling period (after the 13th day). According to the analysis results for angiogenesis, we realized that the efficacy of PSFF on repairing wound was reflected in coordination between infiltration of cells in the pores and angiogenesis, and in timeliness of the newly formed blood vessels’ extention from the interface layer to the material layer.

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Morphological Characterization of Mycobacteriophage R1

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051281 ◽

1974 ◽

Vol 32 ◽

pp. 254-255

Author(s):

B. L. Soloff ◽

T. A. Rado

Keyword(s):

Carbon Source ◽

Stationary Phase ◽

Growth Phase ◽

Minimal Medium ◽

Morphological Characterization ◽

Logarithmic Growth Phase ◽

Complete Lysis ◽

Lysogenic Culture ◽

Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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AEM analysis of crystallization in Ti-based amorphous alloys

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100075142 ◽

1983 ◽

Vol 41 ◽

pp. 270-271

Author(s):

A.R. Pelton ◽

A.F. Marshall ◽

Y.S. Lee

Keyword(s):

Magnetic Properties ◽

Amorphous Alloys ◽

Amorphous Materials ◽

Isothermal Annealing ◽

Amorphous Matrix ◽

Nucleation And Growth ◽

Current Interest ◽

Amorphous Films ◽

Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

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