IMMUNE RESPONSES TO CANINE DISTEMPER VIRUS IN JOINT DISEASES OF DOGS

Rheumatology ◽  
1994 ◽  
Vol 33 (1) ◽  
pp. 27-31 ◽  
Author(s):  
C. MAY ◽  
S. D. CARTER ◽  
S. C. BELL ◽  
D. BENNETT
Keyword(s):  
Immune Responses ◽  
Canine Distemper Virus ◽  
Joint Diseases ◽  
Canine Distemper

scholarly journals Nanoparticles of Conformation-stabilized Canine Distemper Virus Hemagglutinin are Highly Immunogenic and Induce Robust Immunity

2020 ◽  
Author(s):  
Hao Feng ◽  
Jingjian Dong ◽  
Yan Chen ◽  
Lili Shi ◽  
Bing Shen ◽  
...  
Keyword(s):  
Immune Responses ◽  
Self Assembly ◽  
Canine Distemper Virus ◽  
Systemic Disease ◽  
Neutralizing Antibody ◽  
Canine Distemper ◽  
Igg Antibody ◽  
Organ Systems ◽  
New Type ◽  
H Protein

Abstract BackgroundCanine distemper virus (CDV) infection of ferrets, dogs, and giant pandas causes an acute systemic disease involving multiple organ systems, including the respiratory tract, lymphoid system, and central nervous system. In this study, we tested a new type candidate CDV vaccine–CDV nanoparticles–based on hemagglutinin protein. MethodsThe nanoparticles were generated from conformation-stabilized CDV hemagglutinin tetramers. Immune responses against CDV were evaluated in mice. Immunization was initiated 6 weeks after birth and boosted twice with 4-week intervals. The blood and mucosal samples were collected 2 weeks after each immunization. ResultsVaccination with CDV nanoparticles elicited high levels of IgG antibody titers in mice (approximately seven- to eight fold higher than that obtained with soluble CDV H protein), as well as mucosal immune responses, and developed increased CDV-specific neutralizing antibody. The mice that received nanoparticles showed significantly higher IFN-γ- and IL-4-secreting cell population in the spleen and lymph node compared with mice immunized with soluble H protein. The co-stimulatory molecular expression of CD80 and CD86 on the surface of DCs were also upregulated. ConclusionThe results demonstrate that self-assembly into nanoparticles can increase the immunogenicity of vaccine antigens, and nanoparticles assembled from conformation-stabilized CDV H protein has the potential to serve as a new type CDV vaccine.

A recombinant canine distemper virus expressing a modified rabies virus glycoprotein induces immune responses in mice

Virus Genes ◽  
2015 ◽  
Vol 50 (3) ◽  
pp. 434-441 ◽  
Author(s):  
Zhili Li ◽  
Jigui Wang ◽  
Daoli Yuan ◽  
Shuang Wang ◽  
Jiazeng Sun ◽  
...  
Keyword(s):  
Immune Responses ◽  
Rabies Virus ◽  
Canine Distemper Virus ◽  
Canine Distemper ◽  
Rabies Virus Glycoprotein ◽  
Virus Glycoprotein

scholarly journals Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Jingjian Dong ◽  
Yan Chen ◽  
Lili Shi ◽  
Bing Shen ◽  
Xianliang Sun ◽  
...  
Keyword(s):  
Immune Responses ◽  
Self Assembly ◽  
Canine Distemper Virus ◽  
Systemic Disease ◽  
Neutralizing Antibody ◽  
Multiple Organ ◽  
Canine Distemper ◽  
Igg Antibody ◽  
Organ Systems ◽  
H Protein

Abstract Background Canine distemper virus (CDV) infection of ferrets, dogs, and giant pandas causes an acute systemic disease involving multiple organ systems, including the respiratory tract, lymphoid system, and central nervous system. In this study, we tested a new candidate CDV vaccine-CDV nanoparticles-based on hemagglutinin protein. Methods The nanoparticles were generated from conformation-stabilized CDV hemagglutinin tetramers. Immune responses against CDV were evaluated in mice. Immunization was initiated 6 weeks after birth and boosted two times with 4-week intervals. The blood and mucosal samples were collected 2 weeks after each immunization. Results Vaccination with CDV nanoparticles elicited high levels of IgG antibody titers in mice (approximately sevenfold to eightfold higher than that obtained with soluble CDV H protein) and mucosal immune responses and developed increased CDV-specific neutralizing antibody. The mice that received nanoparticles showed significantly higher IFN-γ- and IL-4-secreting cell population in the spleen and lymph node compared with mice immunized with soluble H protein. The co-stimulatory molecular expression of CD80 and CD86 on the surface of DCs was also upregulated. Conclusion The results demonstrate that self-assembly into nanoparticles can increase the immunogenicity of vaccine antigens, and nanoparticles assembled from conformation-stabilized CDV H protein can serve as a new CDV vaccine.

scholarly journals Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity against Both Pathogens

2017 ◽  
Vol 91 (8) ◽  
Author(s):  
Renata da Fontoura Budaszewski ◽  
Andrew Hudacek ◽  
Bevan Sawatsky ◽  
Beate Krämer ◽  
Xiangping Yin ◽  
...  
Keyword(s):  
Immune Responses ◽  
Fusion Protein ◽  
Rabies Virus ◽  
Canine Distemper Virus ◽  
Canine Distemper ◽  
Wild Type ◽  
Attachment Protein ◽  
Recombinant Viruses ◽  
Virus Particles ◽  
Residual Virulence

ABSTRACT The development of multivalent vaccines is an attractive methodology for the simultaneous prevention of several infectious diseases in vulnerable populations. Both canine distemper virus (CDV) and rabies virus (RABV) cause lethal disease in wild and domestic carnivores. While RABV vaccines are inactivated, the live-attenuated CDV vaccines retain residual virulence for highly susceptible wildlife species. In this study, we developed recombinant bivalent vaccine candidates based on recombinant vaccine strain rabies virus particles, which concurrently display the protective CDV and RABV glycoprotein antigens. The recombinant viruses replicated to near-wild-type titers, and the heterologous glycoproteins were efficiently expressed and incorporated in the viral particles. Immunization of ferrets with beta-propiolactone-inactivated recombinant virus particles elicited protective RABV antibody titers, and animals immunized with a combination of CDV attachment protein- and fusion protein-expressing recombinant viruses were protected from lethal CDV challenge. However, animals that were immunized with only a RABV expressing the attachment protein of CDV vaccine strain Onderstepoort succumbed to infection with a more recent wild-type strain, indicating that immune responses to the more conserved fusion protein contribute to protection against heterologous CDV strains. IMPORTANCE Rabies virus and canine distemper virus (CDV) cause high mortality rates and death in many carnivores. While rabies vaccines are inactivated and thus have an excellent safety profile and high stability, live-attenuated CDV vaccines can retain residual virulence in highly susceptible species. Here we generated recombinant inactivated rabies viruses that carry one of the CDV glycoproteins on their surface. Ferrets immunized twice with a mix of recombinant rabies viruses carrying the CDV fusion and attachment glycoproteins were protected from lethal CDV challenge, whereas all animals that received recombinant rabies viruses carrying only the CDV attachment protein according to the same immunization scheme died. Irrespective of the CDV antigens used, all animals developed protective titers against rabies virus, illustrating that a bivalent rabies virus-based vaccine against CDV induces protective immune responses against both pathogens.

Humoral and cell-mediated immune responses in DNA immunized mink challenged with wild-type canine distemper virus

Vaccine ◽  
2009 ◽  
Vol 27 (35) ◽  
pp. 4791-4797 ◽  
Author(s):  
Line Nielsen ◽  
Mette Søgaard ◽  
Peter Karlskov-Mortensen ◽  
Trine Hammer Jensen ◽  
Tove Dannemann Jensen ◽  
...  
Keyword(s):  
Immune Responses ◽  
Canine Distemper Virus ◽  
Canine Distemper ◽  
Wild Type

Effects of chloramphenicol on the development of immune responses to canine distemper virus in Beagle pups

1982 ◽  
Vol 5 (3) ◽  
pp. 177-185 ◽  
Author(s):  
P. L. NARA ◽  
L. E. DAVIS ◽  
L. H. LAUERMAN ◽  
JANICE E. COYLE-DENNIS ◽  
J. PAUL
Keyword(s):  
Immune Responses ◽  
Canine Distemper Virus ◽  
Canine Distemper

scholarly journals Self-assembling ferritin nanoparticles coupled with linear sequences from canine distemper virus haemagglutinin protein elicit robust immune responses

2022 ◽  
Vol 20 (1) ◽  
Author(s):  
Bo Wang ◽  
Shuang Li ◽  
Yongbo Qiao ◽  
Yu Fu ◽  
Jiaojiao Nie ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Prokaryotic Expression ◽  
Canine Distemper Virus ◽  
Large Scale ◽  
High Efficiency ◽  
Control Group ◽  
Canine Distemper ◽  
Cytokine Detection ◽  
Self Assembling

Abstract Background Canine distemper virus (CDV), which is highly infectious, has caused outbreaks of varying scales in domestic and wild animals worldwide, so the development of a high-efficiency vaccine has broad application prospects. Currently, the commercial vaccine of CDV is an attenuated vaccine, which has the disadvantages of a complex preparation process, high cost and safety risk. It is necessary to develop a safe and effective CDV vaccine that is easy to produce on a large scale. In this study, sequences of CDV haemagglutinin (HA) from the Yanaka strain were aligned, and three potential linear sequences, termed YaH3, YaH4, and YaH5, were collected. To increase the immunogenicity of the epitopes, ferritin was employed as a self-assembling nanoparticle element. The ferritin-coupled forms were termed YaH3F, YaH4F, and YaH5F, respectively. A full-length HA sequence coupled with ferritin was also constructed as a DNA vaccine to compare the immunogenicity of nanoparticles in prokaryotic expression. Result The self-assembly morphology of the proteins from prokaryotic expression was verified by transmission electron microscopy. All the proteins self-assembled into nanoparticles. The expression of the DNA vaccine YaHF in HEK-293T cells was also confirmed in vitro. After subcutaneous injection of epitope nanoparticles or intramuscular injection of DNA YaHF, all vaccines induced strong serum titres, and long-term potency of antibodies in serum could be detected after 84 days. Strong anti-CDV neutralizing activities were observed in both the YaH4F group and YaHF group. According to antibody typing and cytokine detection, YaH4F can induce both Th1 and Th2 immune responses. The results of flow cytometry detection indicated that compared with the control group, all the immunogens elicited an increase in CD3. Simultaneously, the serum antibodies induced by YaH4F and YaHF could significantly enhance the ADCC effect compared with the control group, indicating that the antibodies in the serum effectively recognized the antigens on the cell surface and induced NK cells to kill infected cells directly. Conclusions YaH4F self-assembling nanoparticle obtained by prokaryotic expression has no less of an immune effect than YaHF, and H4 has great potential to become a key target for the easy and rapid preparation of epitope vaccines. Graphical Abstract

scholarly journals Intratumoral Canine Distemper Virus Infection Inhibits Tumor Growth by Modulation of the Tumor Microenvironment in a Murine Xenograft Model of Canine Histiocytic Sarcoma

2021 ◽  
Vol 22 (7) ◽  
pp. 3578
Author(s):  
Federico Armando ◽  
Adnan Fayyad ◽  
Stefanie Arms ◽  
Yvonne Barthel ◽  
Dirk Schaudien ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Virus Infection ◽  
Tumor Growth ◽  
Canine Distemper Virus ◽  
Xenograft Model ◽  
Histiocytic Sarcoma ◽  
Oncolytic Viruses ◽  
Canine Distemper ◽  
Murine Xenograft Model ◽  
The Impact

Histiocytic sarcomas refer to highly aggressive tumors with a poor prognosis that respond poorly to conventional treatment approaches. Oncolytic viruses, which have gained significant traction as a cancer therapy in recent decades, represent a promising option for treating histiocytic sarcomas through their replication and/or by modulating the tumor microenvironment. The live attenuated canine distemper virus (CDV) vaccine strain Onderstepoort represents an attractive candidate for oncolytic viral therapy. In the present study, oncolytic virotherapy with CDV was used to investigate the impact of this virus infection on tumor cell growth through direct oncolytic effects or by virus-mediated modulation of the tumor microenvironment with special emphasis on angiogenesis, expression of selected MMPs and TIMP-1 and tumor-associated macrophages in a murine xenograft model of canine histiocytic sarcoma. Treatment of mice with xenotransplanted canine histiocytic sarcomas using CDV induced overt retardation in tumor progression accompanied by necrosis of neoplastic cells, increased numbers of intratumoral macrophages, reduced angiogenesis and modulation of the expression of MMPs and TIMP-1. The present data suggest that CDV inhibits tumor growth in a multifactorial way, including direct cell lysis and reduction of angiogenesis and modulation of MMPs and their inhibitor TIMP-1, providing further support for the concept of its role in oncolytic therapies.

scholarly journals Drivers of canine distemper virus exposure in dogs at a wildlife interface in Janos, Mexico

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Rocío Almuna ◽  
Andrés M. López‐Pérez ◽  
Rosa E. Sarmiento ◽  
Gerardo Suzán
Keyword(s):  
Canine Distemper Virus ◽  
Canine Distemper ◽  
Virus Exposure
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