Effect of Rehydration on in Vitro Germination of Loblolly Pine Pollen

1990 ◽  
Vol 14 (1) ◽  
pp. 48-51 ◽  
Author(s):  
J. B. Jett ◽  
L. John Frampton

Abstract At moisture contents of less than 15%, loblolly pine (Pinus taeda L.) pollen displayed a marked sensitivity to rehydration prior to in vitro germination testing. At moisture contents above 15%, percent germination was relatively insensitive to increasing moisture content. The relationshipbetween length of rehydration time and pollen moisture content was highly significant. However, the commonly used 16-hour rehydration period is longer than is necessary since 1-2 hours of rehydration adequately ensure peak germination. Shortening of the rehydration period allows operationaltree improvement programs to test a greater number of pollen lots in a given period of time. South. J. App. For. 14(1):48-51.

2014 ◽  
Vol 57 (4) ◽  
pp. 555-562
Author(s):  
Deneke H. Mariam ◽  
David L. Bramlett ◽  
John E. Mayfield ◽  
William V. Dashek

<em>Pinus taeda</em> L. (loblolly pine), a commercial timber three of the Southeastern United States, provides a major component of the region's forest resources. We cultured loblolly ovules for in vitro fertilization. This procedure was assisted by quantifying time-dependent alterations of in situ ovular RNA, DNA and total protein (Jan-Aug, 1985) contents for ovules (yr 2 of reproductive cycle). Cold-hot, TCA extracted macromolecules were quantified by colorimetry and UV spectroscopy. Total protein was about 0.4 µg per 100 ovules x 10<sup>4</sup> (Jan-Apr) and except for July increased to 3.6 µg per 100 ovules x 10<sup>4</sup> by August. In contrast, RNA "dropped" from 3 to about I µg per 100 ovules x 10<sup>2</sup> (Jan-Mar) and then rose to 7 µg per 100 ovules x 10<sup>2</sup> by July. The DNA climbed from about I to above 8 µg per 100 ovules x 10<sup>2</sup> (Jan-Mar) and then plummeted to I µg per 100 ovules x 10<sup>2</sup> (Apr-June). The observed alterations may reflect ovule morphogenesis.


1984 ◽  
Vol 8 (4) ◽  
pp. 189-193 ◽  
Author(s):  
Stuart E. Duba ◽  
James F. Goggans ◽  
Richard M. Patterson

Abstract Eight Alabama loblolly pine (Pinus taeda L.) seed sources were compared by establishing one plantation near each source of seed. On the basis of growth after 14 years, the eight sources segregated into three groups that corresponded with southern, central, and northern geographic areas. Southern sources generally had the largest heights and volumes, while central sources were intermediate and northern sources smallest. Although some source-by-location interactions did occur, southern sources could be utilized in the central portion of the state and central sources could be utilized in northern areas to provide above-average growth. Implications for tree improvement programs are made.


2012 ◽  
Vol 42 (3) ◽  
pp. 574-584 ◽  
Author(s):  
Finto Antony ◽  
Laurence R. Schimleck ◽  
Richard F. Daniels

Conventionally, increment cores collected at breast height (1.4 m) have been used to measure wood properties of standing loblolly pine (Pinus taeda L.) trees. This height has been used because of the ease of sampling and cost involved in extracting the cores. In this study, the efficacy of a breast-height core to represent whole-tree specific gravity (SG) and moisture content (MC) was examined. The sampling height that best represents whole-tree SG and MC was identified using the correlation between volume-weighted whole-tree SG and MC with SG and MC measurements collected at different heights within a tree. We found a high correlation between observed whole-disk SG and MC with volume-weighted whole-tree SG and MC at most sampling heights. The strength of the correlation followed a skewed parabolic curve form for both wood properties. The strongest correlations were observed between 4.6 and 6.1 m, with marginally lower correlations at the base of the tree, and the weakest correlations were observed towards the tip of the tree. In addition, it was found that the number of stands and trees that need to be sampled to achieve a certain accuracy in overall mean whole-tree SG and MC was greater if breast height was used as a sampling height compared with the most representative sampling heights identified (4.6 m for SG and 6.1 m for MC).


2008 ◽  
Vol 38 (6) ◽  
pp. 1676-1689 ◽  
Author(s):  
Fikret Isik ◽  
Marcia Gumpertz ◽  
Bailian Li ◽  
Barry Goldfarb ◽  
Xuan Sun

Variation in microfibril angle (MFA) (degrees) among loblolly pine ( Pinus taeda L.) full-sib families and clones was investigated using 43 clones from nine full-sib crosses tested at two locations. When the experiments were 12 years old, a total of 316 trees were drilled and 12 mm thick wood increment cores were collected. MFA for each growth ring in the wood core was measured using the SilviScan-2 tool. A quadratic mixed model was fitted to evaluate the MFA variation over different rings. Among the error covariance structures tested in the model, autoregressive order 1 was the best model for producing MFA estimates with the smallest errors. Estimated MFA was about 33° in the pith (ring 1) of the trees and decreased to 18° in the outer wood (ring 11). Full-sib crosses and clones within crosses explained about 12.5% of the total phenotypic variation. Repeatability of full-sib family means (H2f = 0.46) was moderate but repeatability of clone means was high (H2c = 0.79). Although it is possible to improve (decrease) MFA with recurrent selection in tree improvement programs to improve lumber quality, cost efficient and rapid methods for measuring MFA are needed.


2016 ◽  
Vol 74 (6) ◽  
pp. 857-866 ◽  
Author(s):  
O. Oyedeji ◽  
O. Fasina ◽  
S. Adhikari ◽  
T. McDonald ◽  
S. Taylor

1980 ◽  
Vol 58 (1) ◽  
pp. 36-39 ◽  
Author(s):  
E. G. Kuhlman

Root segments of loblolly pine (Pinus taeda L.) were decayed by Heterobasidion annosum (Fr.) Bref. (=Fomes annosus (Fr.) Karst.) over a broad range of wood (33–317%) and soil (36–259%) moisture contents in modified soil-block tests. Maximum weight loss occurred at 50–286% wood moisture content. Decay was inhibited at wood moisture contents over 290%. Water moved rapidly into the root segments by capillarity, and some decay occurred within 2 weeks. Water uptake was faster in inoculated segments than in noninoculated segments at a soil moisture content of 172% of the 0.2 bar (1 bar = 100 kPa) moisture content.


Genetics ◽  
1999 ◽  
Vol 151 (1) ◽  
pp. 321-330 ◽  
Author(s):  
Mitchell M Sewell ◽  
Bradley K Sherman ◽  
David B Neale

Abstract A consensus map for loblolly pine (Pinus taeda L.) was constructed from the integration of linkage data from two unrelated three-generation outbred pedigrees. The progeny segregation data from restriction fragment length polymorphism, random amplified polymorphic DNA, and isozyme genetic markers from each pedigree were recoded to reflect the two independent populations of parental meioses, and genetic maps were constructed to represent each parent. The rate of meiotic recombination was significantly greater for males than females, as was the average estimate of genome length for males {1983.7 cM [Kosambi mapping function (K)]} and females [1339.5 cM(K)]. The integration of individual maps allows for the synthesis of genetic information from independent sources onto a single consensus map and facilitates the consolidation of linkage groups to represent the chromosomes (n = 12) of loblolly pine. The resulting consensus map consists of 357 unique molecular markers and covers ∼1300 cM(K).


2021 ◽  
Vol 491 ◽  
pp. 119176
Author(s):  
Michael A. Blazier ◽  
Thomas Hennessey ◽  
Laurence Schimleck ◽  
Scott Abbey ◽  
Ryan Holbrook ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document