scholarly journals Evaluation of nitrogen-delivery methods for stocker cattle grazing annual ryegrass

Author(s):  
P A Gunter ◽  
M K Mullenix ◽  
L C Burdette ◽  
R B Muntifering

Abstract A 2-yr grazing experiment was conducted to evaluate efficacy of nitrogen (N) fertilization, interseeded legumes and protein supplementation for N delivery to stocker cattle grazing annual ryegrass (Lolium multiflorum). Each yr, 90 steers (initial BW, 241 ± 13 kg) were assigned to the following N-delivery methods, with or without monensin fed in a free-choice mineral supplement as a 5 x 2 factorial arrangement of treatments: ryegrass fertilized with 112 kg N/ha (NFERT); ryegrass interseeded with crimson clover (CC, Trifolium incarnatum); ryegrass interseeded with arrowleaf clover (AC, Trifolium vesiculosum); ryegrass plus distillers dried grains with solubles (DDGS) supplemented at 0.65% BW daily; and ryegrass plus whole cottonseed (WCS) supplemented at 0.65% BW daily. Pastures within the interseeded-clover and protein-supplementation treatments were fertilized with 56 kg N/ha at time of establishment. Steers were weighed every 28 d, and forage mass (FM, kg DM/ha) was measured concurrently using the destructive harvest/disk meter double-sampling method. Each of 30 0.81-ha paddocks was stocked initially with 3 ‘tester’ steers, and stocking density (steers/ha) was adjusted using ‘put-and-take steers’ based on changes in FM and steer BW in order to maintain a uniform forage allowance (FA) of 1 kg DM/kg steer BW. Grazing was discontinued on May 11, 2016 in Yr 1 and May 10, 2017 in Yr 2 following 140 and 84 d of grazing, respectively. Data were analyzed as a completely randomized design with repeated measures for which pasture (n = 3) was the experimental unit. Ionophore inclusion did not affect (P > 0.10) any variable measured. Mean FM differed (P < 0.0001) between yr and among N-delivery methods (P < 0.10), and mean FA differed (P = 0.005) among N-delivery methods. Steer ADG differed among N-delivery methods (P = 0.02) and between yr (P < 0.001), whereas total gain/ha differed (P < 0.0008) among N-delivery methods, but not between yr (P = 0.78). Stocking density differed among N-delivery methods (P = 0.02) and between yr (P < 0.0001), and grazing-days/ha differed between yr (P < 0.0001) and among N-delivery methods (P = 0.001). Results indicate that supplementation with a high-protein by-product feed for cattle grazing annual ryegrass maintained ADG, total gain/ha and grazing-days/ha compared with N-fertilized annual ryegrass, and increased ADG, total gain/ha and grazing-days over interseeded legumes.

2018 ◽  
Vol 96 (suppl_1) ◽  
pp. 36-36
Author(s):  
P A Gunter ◽  
R W Peacock ◽  
R B Muntifering ◽  
M K Mullenix

2016 ◽  
Vol 95 (suppl_1) ◽  
pp. 26-26
Author(s):  
P. A. Gunter ◽  
R. W. Peacock ◽  
R. B. Muntifering ◽  
M. K. Mullenix

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 29-29
Author(s):  
Kynzie McNeill ◽  
Ryan R Reuter ◽  
Paul A Beck

Abstract A 72-d trial was conducted to estimate the combined effect of forage allowance and a fiber-based energy supplement in stocker cattle grazing wheat. Eight pastures (2.7 to 5.1 ha) each contained seven test steers (initial BW = 210 ± 36 kg). Weekly, additional steers were stocked in each pasture in a put and take method to achieve forage allowance of either 2.5 or 5.5 kg forage DM / kg steer BW (4 pastures each). Two pastures in each forage allowance were fed daily with a supplement containing 50% wheat midds and 50% soybean hulls at the rate of 1.5 kg per steer. Forage mass was measured twice weekly using a calibrated rising plate meter. Cattle were weighed weekly on calibrated scales. Data were analyzed using linear regression with pasture as the experimental unit. Mean ADG was 1.36 kg/d. One pasture in the high forage allowance, non-supplemented treatment was removed because we were unable to maintain the designed forage allowance. The interaction of forage allowance and supplement was not significant (P = 0.37) for ADG, and supplementation did not affect ADG (P = 0.20), however increased forage allowance tended to improve ADG (P = 0.06, 0.21 kg ADG per kg forage allowance). To further investigate forage DMI, a 14-d trial began on day 36. Three of the seven test steers in each pasture were randomly chosen and administered 7 ± 0.1 g of TiO2 daily at 0700 as an external marker to estimate fecal output. Forage DMI (at an assumed 72% DM digestibility) averaged 2.1% of midpoint BW, below our expectation. Additional forage allowance tended to improve estimated forage DMI (P= 0.06), but supplement had minimal effect (P = 0.30).


2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 10-11
Author(s):  
Kynzie McNeill ◽  
Ryan R Reuter ◽  
Paul A Beck

Abstract A 72-d trial was conducted to estimate the combined effect of forage allowance and a fiber-based energy supplement in stocker cattle grazing wheat. Eight pastures (2.7 to 5.1 ha) each contained seven test steers (initial BW = 210 ± 36 kg). Weekly, additional steers were stocked in each pasture in a put and take method to achieve forage allowance of either 2.5 or 5.5 kg forage DM / kg steer BW (4 pastures each). Two pastures in each forage allowance were fed daily with a supplement containing 50% wheat midds and 50% soybean hulls at the rate of 1.5 kg per steer. Forage mass was measured twice weekly using a calibrated rising plate meter. Cattle were weighed weekly on calibrated scales. Data were analyzed using linear regression with pasture as the experimental unit. Mean ADG was 1.36 kg/d. One pasture in the high forage allowance, non-supplemented treatment was removed because we were unable to maintain the designed forage allowance. The interaction of forage allowance and supplement was not significant (P = 0.37) for ADG, and supplementation did not affect ADG (P = 0.20), however increased forage allowance tended to improve ADG (P = 0.06, 0.21 kg ADG per kg forage allowance). To further investigate forage DMI, a 14-d trial began on day 36. Three of the seven test steers in each pasture were randomly chosen and administered 7 ± 0.1 g of TiO2 daily at 0700 as an external marker to estimate fecal output. Forage DMI (at an assumed 72% DM digestibility) averaged 2.1% of midpoint BW, below our expectation. Additional forage allowance tended to improve estimated forage DMI (P= 0.06), but supplement had minimal effect (P = 0.30).


2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 20-20
Author(s):  
Sarah M Montgomery ◽  
Brandi Karisch ◽  
Jane A Parish ◽  
Jesse Morrison ◽  
Brian Baldwin

Abstract Annual ryegrass (Lolium multiflorum Lam.) can be divided into two cytotypes: diploid (2n = 2x) and tetraploid (2n = 4x). Polyploid versions of a given plant species result in increased seedling vigor, more robust growth and, potentially, greater yield. While most data suggest no difference in yield between diploid and tetraploid cultivars, there is still potential for weight gain advantages thanks to increased non-structural carbohydrates in tetraploid cells. The objectives of this study were to compare weight gain of cattle grazing either diploid or tetraploid cultivars of annual ryegrass. Two diploid annual ryegrass cultivars (Marshall and Tam 90) and two tetraploid cultivars (Jumbo and Nelson) were planted in 0.81-ha pastures, each replicated four times. Four steers (mean initial BW = 214 kg) were randomly assigned to each pasture for an 82-d grazing period. Body weights were recorded starting at d0 then every 28 days throughout the season. Data were analyzed in the GLM procedure of SAS using a significance level of α = 0.05. There were significant differences between cytotypes with respect to seasonal yield with diploids (9547.90 kg/ha) yielding greater than tetraploids (7762.00 kg/ha). As expected, ADF and NDF fractions were significantly less for tetraploids (ADF; 31.39%, NDF; 31.39%) compared to diploids (ADF; 33.75%, NDF; 53.25%). Tetraploids also contained significantly greater crude protein (13.50%) than diploids (12.90%). Cytotype had no effect on final average daily gain (ADG) (P = 0.9427). However, final ADG was significantly impacted by cultivar (P = 0.0134). Final ADG for Tam 90 (1.10 kg/d) was greater than Marshall (0.92 kg/d). Cattle grazing Nelson (1.04 kg/d) and Jumbo (0.99 kg/d) did not differ. Results show that regardless of differences in seasonal yield and nutritive value between cytotypes, there were no advantages in ADG for cattle grazing either cytotype.


2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Dalton Humphrey ◽  
Spenser Becker ◽  
Jason Lee ◽  
Keith Haydon ◽  
Laura Greiner

Abstract Four hundred and eighty (PIC 337 X 1050, PIC Genus, Hendersonville, TN) pigs were used to evaluate a novel threonine source (ThrPro, CJ America Bio, Fort Dodge, IA) for nursery pigs from approximately 7 to 20 kg body weight (BW). After weaning, pigs were sorted by sex and fed a common diet for 1 wk. Upon completion of the first week, pigs were sorted into randomized complete blocks, equalized by weight, within 16 replications. Pigs were allocated to one of three dietary treatments: positive control (POS)—standard ileal digestible threonine-to-lysine ratio (SID; Thr:Lys) 0.60, negative control (NEG)—SID Thr:Lys ≤0.46, and alternative Thr source (TEST)—SID Thr:Lys 0.60. The alternative Thr source included fermentative biomass and was assumed to contain 75% Thr and a digestibility coefficient of 100% based on the manufacturer’s specifications. All other nutrients met or exceeded the NRC recommendations. Growth and intake data were analyzed as repeated measures with a compound symmetry covariance structure using the MIXED procedure in SAS 9.4 (SAS Institute Inc., Cary, NC) with pen as the experimental unit. Treatment, phase, the interaction between treatment and phase, and block were included as fixed effects in the model. Differences in total removals were tested using Fisher’s Exact Test of PROC FREQ. Results were considered significant at P ≤ 0.05 and considered a trend at P > 0.05 and P ≤ 0.10. During the first 14 d, pigs fed TEST had decreased gain-to-feed ratio (G:F; 0.77 vs. 0.80, P = 0.022) compared to POS and increased G:F (0.77 vs. 0.73, P < 0.001) compared to NEG. Over days 14–28, pigs fed TEST had similar G:F (0.71 vs. 0.70, P = 0.112) compared to POS and increased G:F (0.71 vs. 0.63, P < 0.001) compared to NEG. Overall (days 0–28), pigs fed TEST had similar average daily gain (ADG; 0.47 vs. 0.47 kg/d, P = 0.982) and G:F (0.76 vs. 0.74, P = 0.395) compared to POS and increased ADG (0.47 vs. 0.43 kg/d, P < 0.001) and G:F (0.76 vs. 0.67, P < 0.001) compared to NEG. The average daily feed intake was not significantly different across treatments for the entirety of the study. In conclusion, the replacement of crystalline L-Thr with a novel Thr source resulted in similar growth performance in nursery pigs from approximately 7 to 20 kg.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 152-153
Author(s):  
Aubree M Beenken ◽  
Erin L Deters ◽  
Colten W Dornbach ◽  
Stephanie L Hansen ◽  
Joshua C McCann ◽  
...  

Abstract Ninety-one early-weaned (65 ± 11 d) Angus steers (92 ± 4 kg) were blocked by age to a 2 × 2 factorial examining effects of injectable vitamin C (VC) at weaning and/or prior to transport to the feedlot on antibody titers and growth performance. Injections (20 mL/steer) of VC (250 mg sodium ascorbate/mL) or saline (SAL) were given at time of weaning on d 0 (WEAN) and/or prior to a 6 hr trucking event to a feedlot on d 49 (TRANS). Steers were given booster vaccinations on d 0. Steers were weighed on d 0, 1, 14, 48, 49, 64, 106, and 107. Blood was collected (12 steers/treatment) on d 0, 1, 2, 14, 49 (pre- and post-transit), 50, and 51. Data were analyzed via Proc-Mixed of SAS (experimental unit = steer; n = 22–23/treatment) with fixed effects of block, WEAN, TRANS, and WEAN × TRANS. Plasma ascorbate concentrations for weaning (d 0, 1, and 2) and transit (d 49-pre-trucking, 49-post-trucking, 50, and 51) were analyzed as repeated measures (repeated effect = day). Plasma ascorbate concentrations were greater on d 1 and 2 for steers that received VC at weaning (VC = 19.6, SAL = 8.8 ± 1.26 µM; WEAN × day P < 0.01). Similarly, ascorbate concentrations were greater on d 49 post-trucking, 50, and 51 for steers that received VC pre-transit (TRANS × day P = 0.01). Treatments did not affect bodyweight or average daily gain throughout the trial (P > 0.32). There were no effects of treatment on serum Bovine Viral Diarrhea Virus type 2 antibody titers on d 14 or 51 (P > 0.33). An injection of VC administered to early weaned beef steers at weaning or pre-transit increases plasma ascorbate concentrations but does not improve growth performance or antibody response to vaccination booster.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-167
Author(s):  
Andrea M Osorio ◽  
Kaue T Tonelli Nardi ◽  
Igor Gomes Fávero ◽  
Kaliu G Scaranto Silva ◽  
Kymberly D Coello ◽  
...  

Abstract The effects of a nutritional packet were evaluated on CH4 emissions and apparent total tract nutrient digestibility of feedlot beef steers. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens equipped with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based diet (88% concentrate DM basis) ad libitum for the last 65 d on feed, and received the following treatments: 1) control and 2) a nutritional packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Methane emissions and apparent total tract nutrient digestibility were measured during 3 periods with 5-d of collections each. Gas emissions from steers were measured utilizing the SF6 tracer technique. Feed and fecal samples were collected once and twice (0700 h and 1600 h) daily, respectively, to determine digestibility of nutrients using iNDF as an internal marker. Steer was considered the experimental unit. Data were analyzed as repeated measures using the MIXED procedure of SAS with the fixed effects of treatment, period, and their interaction, and the random effect of block. No treatment × period interactions (P ≥ 0.125) were observed for DMI and any of the CH4 production variables (g/day, g/kg BW0.75, g/nutrient intake, and g/nutrient digested). Moreover, treatments did not affect digestibility of DM, OM, or ADF (P ≥ 0.300); however, digestibility of NDF was increased for treated cattle (P = 0.013), which resulted in a tendency (P = 0.098) to decrease CH4 production in g per kg NDF intake and decreased (P = 0.020) grams CH4 per kg NDF digested. The nutritional packet may be altering ruminal fermentation on intensively managed steers and improving fiber digestibility, which can have benefits on CH4 emission intensity.


2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 64-64
Author(s):  
Taylor C Husz ◽  
Carla Goad ◽  
Ryan R Reuter

Abstract The objective of this research was to determine the effect of competition for a feeding space at an automated supplement feeder on supplement intake behavior. A 2 yr study was conducted; each yr, 128 mixed-breed beef steers (n = 256, initial BW = 245 ± 27.5 kg) were randomly assigned to 8 paddocks. One paddock each yr (n = 16 steers) was selected to have continuous access to the feeder for the duration of the 16 wk trial; this paddock was designated the “tester” paddock. The automated feeder had 4 feeding stations that dispensed supplement after eligible animal’s RFID tags were read by the electronics of the feeder. Steers were limited to 0.50 kg supplement/d. Weekly, additional paddocks were commingled with the tester paddock to increase competition for the feeder. This resulted in a stocking density from 4 to 32 steers per feeding station. For yr 2, one feeding station was disabled, resulting in a stocking density of 5.3 to 42.7 steers per feeding station. Approximately 31% of the steers did not voluntarily use the feeder; therefore, effective competition was less than the study design. Mean weekly supplement intake and GPS location were recorded for steers in the tester paddock. Additionally, pedometer data were collected in yr 2. Weekly mean supplement intake and time spent near the feeder were regressed on actual feeder stocking density with yr as a random variable; wk was the experimental unit (n = 16 wk). Tester steers consumed an average of 0.27 kg supplement/d. Competition for a feeding station numerically reduced (P = 0.01) supplement intake by 5 g/d per steer of additional competition. Steers spent 4.4% of the time within 15 m of the feeder regardless of competition (P = 0.54). As competition increased, steers took more steps (P < 0.01). These data illustrate that in environments similar to this experiment, the feeder can be stocked with at least 20 animals per feeding station with minimal effects on supplement intake.


2019 ◽  
Vol 97 (9) ◽  
pp. 3776-3785 ◽  
Author(s):  
Rebecca K Poole ◽  
Carrisa M Womble ◽  
Matthew H Poore ◽  
Daniel H Poole ◽  
Carrie L Pickworth

Abstract Fescue toxicosis is a multifaceted syndrome common in cattle grazing endophyte-infected tall fescue and is detrimental to growth and performance. Recent research has shown that supplementing protein has the potential to enhance growth performance in weaned steers. Therefore, the objective of this study was to evaluate the effect of supplemental CP on physiological parameters in stocker steers experiencing fescue toxicosis. Thirty-six weaned Angus steers (6 mo of age) stratified by weight (196.1 ± 3.6 kg) were assigned to a 2 × 2 factorial arrangement for 56 d: endophyte-free (EF) seed and 14% CP (EF-14; n = 9), EF seed and 18% CP (EF-18; n = 9), endophyte-infected (EI) seed and 14% CP (EI-14; n = 9), and EI seed and 18% CP (EI-18; n = 9). Steer growth and hemodynamic responses were collected weekly during ergot alkaloid exposure. On day 14 of the trial, iButton temperature data loggers were subcutaneously inserted in the lateral neck region to record hourly body temperature for 42 d. Data were analyzed using PROC MIXED of SAS with repeated measures. No differences were observed in DMI, BW, ADG, F:G, or BCS during the treatment period (P > 0.05). Hair shedding scores, rectal temperatures, surface temperatures, and respiration rates were greater in EI steers compared to EF steers regardless of supplemental CP (P < 0.05). However, subcutaneous body temperature was greater in EI-14 steers (37.94 °C) compared to other steer groups (37.60, 37.68, 37.72 ± 0.04 °C for EF-14, EF-18, and EI-18, respectively; P < 0.05). Prolactin concentrations tended to be greater in EF steers when compared to EI steers (P = 0.07). Heart rate and hematocrit were reduced for EI-18 steers compared to other steer groups (P < 0.05). Caudal artery diameter was reduced in EI-18 steers compared to EI-14 steers (2.60 vs. 2.75 ± 0.05 mm, respectively; P < 0.05) and caudal vein diameter was reduced in EI-18 steers (3.20 mm) compared to all other steer groups (3.36, 3.39, 3.50 mm for EF-14, EF-18, and EI-14, respectively; P < 0.05). However, there was no difference observed in systolic or diastolic blood pressure during the treatment period (P > 0.05). Based on the data, exposure to low to moderate levels of ergot alkaloids during the stocker phase had a negative impact on hemodynamic responses and supplemental CP had minimal impact to alleviate symptoms. Therefore, feeding additional protein above established requirements is not expected to help alleviate fescue toxicosis.


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