Effect of Variation in Amylose Content and Puroindoline Composition on Bread Quality in a Hard Spring Wheat Population

Cadmium (Cd) is a heavy metal that can cause a variety of adverse effects on human health, including cancer. Wheat comprises approximately 20% of the human diet worldwide; therefore, reducing the concentrations of Cd in wheat grain will have significant impacts on the intake of Cd in food products. The tests for measuring the Cd content in grain are costly, and the content is affected significantly by soil pH. To facilitate breeding for low Cd content, this study sought to identify quantitative trait loci (QTL) and associated molecular markers that can be used in molecular breeding. One spring wheat population of 181 doubled haploid lines (DHLs), which was derived from a cross between two hard white spring wheat cultivars “UI Platinum” (UIP) and “LCS Star” (LCS), was assessed for the Cd content in grain in multiple field trials in Southeast Idaho, United States. Three major QTL regions, namely, QCd.uia2-5B, QCd.uia2-7B, and QCd.uia2-7D, were identified on chromosomes 5B, 7B, and 7D, respectively. All genes in these three QTL regions were identified from the NCBI database. However, three genes related to the uptake and transport of Cd were used in the candidate gene analysis. The sequences of TraesCS5B02G388000 (TaHMA3) in the QCd.uia2-5B region and TraesCS7B02G320900 (TaHMA2) and TraesCS7B02G322900 (TaMSRMK3) in the QCd.uia2-7B region were compared between UIP and LCS. TaHMA2 on 7B is proposed for the first time as a candidate gene for grain Cd content in wheat. A KASP marker associated with this gene was developed and it will be further validated in near-isogenic lines via a gene-editing system in future studies.

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Multi-environment QTL analysis using an updated genetic map of a widely distributed Seri × Babax spring wheat population

Molecular Breeding ◽

10.1007/s11032-019-1040-1 ◽

2019 ◽

Vol 39 (9) ◽

Author(s):

Caiyun Liu ◽

Mehdi Khodaee ◽

Marta S. Lopes ◽

Carolina Sansaloni ◽

Susanne Dreisigacker ◽

...

Keyword(s):

Genetic Map ◽

Spring Wheat ◽

Qtl Analysis ◽

Wheat Population

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Earliness per se quantitative trait loci and their interaction with Vrn-B1 locus in a spring wheat population

Molecular Breeding ◽

10.1007/s11032-015-0373-7 ◽

2015 ◽

Vol 35 (9) ◽

Cited By ~ 5

Author(s):

Hua Chen ◽

Muhammad Iqbal ◽

Enid Perez-Lara ◽

Rong-Cai Yang ◽

Curtis Pozniak ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Spring Wheat ◽

Quantitative Trait ◽

Earliness Per Se ◽

Wheat Population ◽

Trait Loci ◽

Per Se

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QTL mapping of adult plant resistance to Ug99 stem rust in the spring wheat population RB07/MN06113-8

Molecular Breeding ◽

10.1007/s11032-015-0362-x ◽

2015 ◽

Vol 35 (8) ◽

Cited By ~ 8

Author(s):

Prabin Bajgain ◽

Matthew N. Rouse ◽

Sridhar Bhavani ◽

James A. Anderson

Keyword(s):

Qtl Mapping ◽

Spring Wheat ◽

Plant Resistance ◽

Stem Rust ◽

Adult Plant Resistance ◽

Adult Plant ◽

Wheat Population

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Variability in arabinoxylan, xylanase activity, and xylanase inhibitor levels in hard spring wheat

CFW Plexus ◽

10.1094/cplex-2012-1114-01w ◽

2012 ◽

Author(s):

Mihiri Mendis ◽

Jan Delcour ◽

Kurt Gebruers ◽

Jae Ohn ◽

Steven Meinhardt ◽

...

Keyword(s):

Spring Wheat ◽

Xylanase Activity ◽

Xylanase Inhibitor ◽

Hard Spring Wheat

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Physiological and Yield Responses of Spring Wheat Cultivars under Realistic and Acute Levels of Ozone

Atmosphere ◽

10.3390/atmos12111392 ◽

2021 ◽

Vol 12 (11) ◽

pp. 1392

Author(s):

Nivedita Chaudhary ◽

David J. Bonfil ◽

Eran Tas

Keyword(s):

Spring Wheat ◽

Eastern Mediterranean ◽

Physiological Responses ◽

Wheat Cultivars ◽

Ambient Conditions ◽

Mixing Ratios ◽

Yield And Quality ◽

Stomatal Responses ◽

Yield Responses ◽

Hard Spring Wheat

Tropospheric ozone (O3) is widely recognized as the cause of substantial yield and quality reduction in crops. Most of the previous studies focused on the exposure of wheat cultivars to elevated O3 levels. Our main objectives were to: (i) investigate the consistency of wheat cultivars’ physiological responses across two different realistic O3 levels; and (ii) compare these physiological responses with those under short acute O3 exposure. Three commercially available hard spring wheat cultivars bred under semiarid and Eastern Mediterranean conditions were exposed to two different O3 levels during two consecutive seasons (2016–2018)—36 and 71 ppbv 7 h mean O3 mixing ratios in open-top chambers. The results were compared to those following short acute O3 exposure (102.8 ppbv, 7 h mean for 10 days) in a greenhouse. Non-stomatal responses were significantly more pronounced than stomatal responses in all cultivars under different levels of O3. The specific cultivar was observed as the most O3-tolerant under all experiments. The fact that the same cultivar was found remarkably tolerant to the local semiarid ambient conditions according to other studies and to O3 exposure based on the present study supports a link between cultivar resistance to drought conditions and O3.

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Milling and baking quality of hexaploid spring wheat starch synthase IIa ( ssIIa ) mutants with elevated amylose content

Cereal Chemistry ◽

10.1002/cche.10153 ◽

2019 ◽

Vol 96 (3) ◽

pp. 532-544

Author(s):

Andrew C. Hogg ◽

Michael J. Giroux

Keyword(s):

Spring Wheat ◽

Amylose Content ◽

Starch Synthase ◽

Wheat Starch ◽

Baking Quality

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Puroindoline allele diversity in Canadian and northern US hard spring wheat varieties differing in kernel hardness

Canadian Journal of Plant Science ◽

10.4141/cjps07168 ◽

2008 ◽

Vol 88 (5) ◽

pp. 873-883 ◽

Cited By ~ 5

Author(s):

M. A. Matus-Cádiz ◽

C. J. Pozniak ◽

P. Hucl

Keyword(s):

Triticum Aestivum ◽

Spring Wheat ◽

Point Mutations ◽

Allelic Diversity ◽

Triticum Aestivum L ◽

Kernel Hardness ◽

Wheat Varieties ◽

Allele Composition ◽

End Use ◽

Hard Spring Wheat

Kernel hardness, one of the most important factors in determining the end-use suitability of wheat (Triticum aestivum L.), is largely influenced by puroindoline proteins a (PINA) and b (PINB). Soft texture is wildtype (Pina-D1a/Pinb-D1a) with hard texture being determined by either Pina-D1 gene deletion or point mutations in Pinb-D1a. The objectives of this study were to determine kernel hardness indices (HI) and Pina-D1/Pinb-D1 allelic diversity in a diverse set of 81 Canadian (representing eight wheat classes) and 49 US hard spring wheat varieties. Varieties were grouped into two experimental sets grown in replicated trials in 2004–2006 at Saskatoon, SK. Variation existed among varieties with HI means ranging from 21.7 (CDC Zorba) to 72.7 (AC Morse) in exp. 1 and from 25.0 (HY320) to 66.1 (Norlander) in exp. 2. Only AC Andrew, CDC Zorba, and Red Fife were soft kernel textured and carried the wildtype Pina-D1a/Pinb-D1a alleles. The majority of Canadian varieties were Pina-D1a/Pinb-D1b (69%), while a greater frequency of US varieties was Pina-D1b/Pinb-D1a (55%). Only four varieties, all from Quebec-based breeding programs, carried the Pinb-D1c allele. On average, varieties with the Pina-D1b allele had significantly harder kernels than those carrying the Pinb-D1b mutation. Durum wheat (T. turgidum L. var durum) varieties, included as hard-kernelled controls, possessed significantly harder kernels (average HI = 71.2) than spring wheat varieties with Pina-D1b (65.3). Varieties carrying Pina-D1b (null PINA) were harder than those carrying hardness mutations at the Pinb-D1 locus, but considerable overlap in hardness was evident among genotypic classes. This work represents the first extensive Pin genotyping combined with HI phenotyping survey of Canadian wheat varieties, which is expected to aid breeders in understanding how Pina-D1/Pinb-D1 allele composition is currently associated with kernel hardness across Canadian wheat classes. Key words: Triticum aestivum, kernel hardness, puroindolines

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