scholarly journals Bioguided Isolation, Characterization, and Biotransformation by Fusarium verticillioides of Maize Kernel Compounds That Inhibit Fumonisin Production

2014 ◽  
Vol 27 (10) ◽  
pp. 1148-1158 ◽  
Author(s):  
Vessela Atanasova-Penichon ◽  
Stéphane Bernillon ◽  
Gisèle Marchegay ◽  
Aurélia Lornac ◽  
Laetitia Pinson-Gadais ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Inhibitory Activity ◽  
Fusarium Verticillioides ◽  
Fusarium Ear Rot ◽  
Ear Rot ◽  
In Planta ◽  
Maize Genotypes ◽  
Maize Genotype ◽  
Rot Disease ◽  
Susceptible Group

Fusarium verticillioides infects maize ears, causing ear rot disease and contamination of grain with fumonisin mycotoxins. This contamination can be reduced by the presence of bioactive compounds in kernels that are able to inhibit fumonisin biosynthesis. To identify such compounds, we used kernels from a maize genotype with moderate susceptibility to F. verticillioides, harvested at the milk-dough stage (i.e., when fumonisin production initiates in planta), and applied a bioguided fractionation approach. Chlorogenic acid was the most abundant compound in the purified active fraction and its contribution to fumonisin inhibitory activity was up to 70%. Moreover, using a set of maize genotypes with different levels of susceptibility, chlorogenic acid was shown to be significantly higher in immature kernels of the moderately susceptible group. Altogether, our data indicate that chlorogenic acid may considerably contribute to either maize resistance to Fusarium ear rot, fumonisin accumulation, or both. We further investigated the mechanisms involved in the inhibition of fumonisin production by chlorogenic acid and one of its hydrolyzed products, caffeic acid, by following their metabolic fate in supplemented F. verticillioides broths. Our data indicate that F. verticillioides was able to biotransform these phenolic compounds and that the resulting products can contribute to their inhibitory activity.

Variation in pathogenicity of Fusarium verticillioides and resistance of maize genotypes to Fusarium ear rot

2018 ◽  
Vol 51 (17-18) ◽  
pp. 939-950
Author(s):  
Olumayowa Mary Olowe ◽  
Ayodele Adegboyega Sobowale ◽  
Odunayo Joseph Olawuyi ◽  
Adegboyega Christopher Odebode
Keyword(s):  
Fusarium Verticillioides ◽  
Fusarium Ear Rot ◽  
Ear Rot ◽  
Maize Genotypes

scholarly journals The Effect of Fusarium verticillioides Fumonisins on Fatty Acids, Sphingolipids, and Oxylipins in Maize Germlings

2021 ◽  
Vol 22 (5) ◽  
pp. 2435
Author(s):  
Marzia Beccaccioli ◽  
Manuel Salustri ◽  
Valeria Scala ◽  
Matteo Ludovici ◽  
Andrea Cacciotti ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fusarium Verticillioides ◽  
Fungal Growth ◽  
Defense Responses ◽  
Ceramide Synthase ◽  
Ear Rot ◽  
In Planta ◽  
Disease Symptoms ◽  
The Impact

Fusarium verticillioides causes multiple diseases of Zea mays (maize) including ear and seedling rots, contaminates seeds and seed products worldwide with toxic chemicals called fumonisins. The role of fumonisins in disease is unclear because, although they are not required for ear rot, they are required for seedling diseases. Disease symptoms may be due to the ability of fumonisins to inhibit ceramide synthase activity, the expected cause of lipids (fatty acids, oxylipins, and sphingolipids) alteration in infected plants. In this study, we explored the impact of fumonisins on fatty acid, oxylipin, and sphingolipid levels in planta and how these changes affect F. verticillioides growth in maize. The identity and levels of principal fatty acids, oxylipins, and over 50 sphingolipids were evaluated by chromatography followed by mass spectrometry in maize infected with an F. verticillioides fumonisin-producing wild-type strain and a fumonisin-deficient mutant, after different periods of growth. Plant hormones associated with defense responses, i.e., salicylic and jasmonic acid, were also evaluated. We suggest that fumonisins produced by F. verticillioides alter maize lipid metabolism, which help switch fungal growth from a relatively harmless endophyte to a destructive necrotroph.

scholarly journals Molecular Insights into Defense Responses of Vietnamese Maize Varieties to Fusarium verticillioides Isolates

2021 ◽  
Vol 7 (9) ◽  
pp. 724
Author(s):  
Trang Minh Tran ◽  
Maarten Ameye ◽  
Sofie Landschoot ◽  
Frank Devlieghere ◽  
Sarah De Saeger ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Fusarium Verticillioides ◽  
Gene Expression Profiles ◽  
Defense Responses ◽  
Ear Rot ◽  
In Planta ◽  
Resistant Lines ◽  
Maize Varieties ◽  
Pathogenesis Related ◽  
Related Proteins

Fusarium ear rot (FER) caused by Fusarium verticillioides is one of the main fungal diseases in maize worldwide. To develop a pathogen-tailored FER resistant maize line for local implementation, insights into the virulence variability of a residing F. verticillioides population are crucial for developing customized maize varieties, but remain unexplored. Moreover, little information is currently available on the involvement of the archetypal defense pathways in the F. verticillioides–maize interaction using local isolates and germplasm, respectively. Therefore, this study aims to fill these knowledge gaps. We used a collection of 12 F. verticillioides isolates randomly gathered from diseased maize fields in the Vietnamese central highlands. To assess the plant’s defense responses against the pathogens, two of the most important maize hybrid genotypes grown in this agro-ecological zone, lines CP888 and Bt/GT NK7328, were used. Based on two assays, a germination and an in-planta assay, we found that line CP888 was more susceptible to the F. verticillioides isolates when compared to line Bt/GT NK7328. Using the most aggressive isolate, we monitored disease severity and gene expression profiles related to biosynthesis pathways of salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA), benzoxazinoids (BXs), and pathogenesis-related proteins (PRs). As a result, a stronger induction of SA, JA, ABA, BXs, and PRs synthesizing genes might be linked to the higher resistance of line Bt/GT NK7328 compared to the susceptible line CP888. All these findings could supply valuable knowledge in the selection of suitable FER resistant lines against the local F. verticllioides population and in the development of new FER resistant germplasms.

scholarly journals Rhamnolipid Biosurfactant against Fusarium verticillioides to Control Stalk and Ear Rot Disease of Maize

2016 ◽  
Vol 7 ◽  
Author(s):  
Siddhartha N. Borah ◽  
Debahuti Goswami ◽  
Hridip K. Sarma ◽  
Swaranjit S. Cameotra ◽  
Suresh Deka
Keyword(s):  
Fusarium Verticillioides ◽  
Ear Rot ◽  
Rot Disease ◽  
Rhamnolipid Biosurfactant

Association mapping and genomic prediction for ear rot disease caused by Fusarium verticillioides in a tropical maize germplasm

Crop Science ◽  
2020 ◽  
Vol 60 (6) ◽  
pp. 2867-2881
Author(s):  
Maurício Carlos Kuki ◽  
Ronald José Barth Pinto ◽  
Filipe Augusto Bengosi Bertagna ◽  
Dauri José Tessmann ◽  
Antônio Teixeira do Amaral ◽  
...  
Keyword(s):  
Association Mapping ◽  
Genomic Prediction ◽  
Fusarium Verticillioides ◽  
Ear Rot ◽  
Tropical Maize ◽  
Maize Germplasm ◽  
Rot Disease

scholarly journals Fumonisin Production by Fusarium verticillioides in Maize Genotypes Cultivated in Different Environments

Toxins ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 215 ◽  
Author(s):  
Oelton Ferreira Rosa Junior ◽  
Mateus Sunti Dalcin ◽  
Vitor L. Nascimento ◽  
Fernando Machado Haesbaert ◽  
Talita Pereira de Souza Ferreira ◽  
...  
Keyword(s):  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Human Consumption ◽  
Ear Rot ◽  
Maize Hybrids ◽  
High Severity ◽  
Maize Genotypes ◽  
Spray Inoculation ◽  
Corn Products ◽  
High Concentrations

Fumonisins are mycotoxins (MTs) produced mainly by the fungus Fusarium verticillioides, the main pathogens of maize which cause ear rot. The aim of this work was to evaluate some factors that may lead to high fumonisin production by F. verticillioides in maize grains, correlating the pathogen inoculation method with different genotypes grown in four Brazilian states. Experiments were conducted in 2015–2016 in maize crops from experimental maize fields located in four distinct states of Brazil. Results showed that contamination by fumonisin mycotoxins occurred even on symptomatic or asymptomatic grains. In all municipalities, the samples showed levels of fumonisin B1 that were higher than would be tolerable for the human consumption of corn products (the current tolerance limit for fumonisin is 1.5 μg g−1). High severity of grains infected with F. verticillioides does not always show high concentrations of fumonisins. Environments with higher temperatures may influence the production of high concentrations of fumonisin in maize hybrids. Spray inoculation methods and inoculation at the center of spikes did not influence fumonisin concentrations. Results showed that the hybrids P3630H, P32R48 and P3250 presented higher disease severity, as well as higher mycotoxin levels in the studied locations with higher temperatures.

scholarly journals Evaluation of Food-Grade Dent Corn Hybrids for Severity of Fusarium Ear Rot and Fumonisin Accumulation in Grain

Plant Disease ◽  
2005 ◽  
Vol 89 (3) ◽  
pp. 291-297 ◽  
Author(s):  
C. E. Kleinschmidt ◽  
M. J. Clements ◽  
C. M. Maragos ◽  
J. K. Pataky ◽  
D. G. White
Keyword(s):  
Fusarium Verticillioides ◽  
Fusarium Ear Rot ◽  
Ear Rot ◽  
Natural Conditions ◽  
Corn Hybrids ◽  
Food Grade ◽  
Human Food ◽  
Inoculation Technique ◽  
Dent Corn ◽  
High Concentrations

Fumonisins produced by Fusarium verticillioides (syn = F. moniliforme) and F. proliferatum have been associated with potentially serious toxicoses of animals and humans. Thus, hybrids with low fumonisin accumulation in grain will be valuable for the production of corn-based human food products. We evaluated 68 food-grade dent corn hybrids for severity of Fusarium ear rot and fumonisin accumulation in grain in inoculated trials in Urbana, IL in 2000 and 2001. Our inoculation technique was successful in initiating fumonisin accumulation that allowed discrimination among hybrids. We identified several hybrids that could have acceptable levels (<4 μg/g) of fumonisin accumulation in Illinois in most years. Twenty-six hybrids with low or high fumonisin accumulation in 2000 were reevaluated in noninoculated trials at three locations in Illinois in 2001. Fumonisin concentration in grain at all three locations was relatively low; thus, separation of hybrids was poor. At two locations, those hybrids with the highest fumonisin concentration in grain also had high concentrations following inoculation. However, one hybrid that had relatively low fumonisin concentration following inoculation had unacceptable levels of fumonisin (5 μg/g) in natural conditions. Therefore, hybrids need to be evaluated by inoculation and further evaluated at locations where the environment favors fumonisin accumulation.

scholarly journals Fusarium verticillioides induces maize-derived ethylene to promote virulence by engaging fungal G-protein signaling

2021 ◽  
Author(s):  
Yong-Soon Park ◽  
Eli J Borrego ◽  
Xiquan Gao ◽  
Shawn A Christensen ◽  
Eric Schmelz ◽  
...  
Keyword(s):  
G Protein ◽  
Fusarium Verticillioides ◽  
G Protein Signaling ◽  
Protein Signaling ◽  
Fusarium Ear Rot ◽  
Ear Rot ◽  
Knock Out ◽  
Seed Colonization ◽  
Meta Analyses ◽  
Exogenous Treatment

Seed maceration and contamination with mycotoxin fumonisin inflicted by Fusarium verticillioides is major disease of concern for maize producers world-wide. Meta-analyses of QTL for Fusarium ear rot resistance uncovered several ethylene (ET) biosynthesis and signaling genes within them, implicating ET in maize interactions with F. verticillioides. We tested this hypothesis using maize knock-out mutants of the 1-aminocyclopropane-1-carboxylate (ACC) synthases, ZmACS2 and ZmACS6. Infected wild-type seed emitted five-fold higher ET levels compared to controls, whereas ET was abolished in the acs2 and acs6 single and double mutants. The mutants supported reduced fungal biomass, conidia and fumonisin content. Normal susceptibility was restored in the acs6 mutant with exogenous treatment of ET precursor, ACC. Subsequently, we showed that fungal G-protein signaling is required for virulence via induction of maize-produced ET. F. verticillioides Gβ subunit and two regulators of G-protein signaling mutants displayed reduced seed colonization and decreased ET levels. These defects were rescued by exogenous application of ACC. We concluded that pathogen-induced ET facilitates F. verticillioides colonization of seed, and in turn host ET production is manipulated via G-protein signaling of F. verticillioides to facilitate pathogenesis.

scholarly journals Complex Genetic System Involved in Fusarium Ear Rot Resistance in Maize as Revealed by GWAS, Bulked Sample Analysis, and Genomic Prediction

Plant Disease ◽  
2020 ◽  
Vol 104 (6) ◽  
pp. 1725-1735 ◽  
Author(s):  
Zifeng Guo ◽  
Cheng Zou ◽  
Xiaogang Liu ◽  
Shanhong Wang ◽  
Wen-Xue Li ◽  
...  
Keyword(s):  
Confidence Level ◽  
Genomic Prediction ◽  
Complex Traits ◽  
Genome Wide Association Study ◽  
Fusarium Verticillioides ◽  
Chromosome 1 ◽  
Nucleotide Polymorphisms ◽  
Fusarium Ear Rot ◽  
Ear Rot ◽  
Sample Analysis

Fusarium ear rot (FER) caused by Fusarium verticillioides is one of the most prevalent maize diseases in China and worldwide. Resistance to FER is a complex trait controlled by multiple genes highly affected by environment. In this paper, genome-wide association study (GWAS), bulked sample analysis (BSA), and genomic prediction were performed for understanding FER resistance using 509 diverse inbred lines, which were genotyped by 37,801 high-quality single-nucleotide polymorphisms (SNPs). Ear rot evaluation was performed using artificial inoculation in four environments in China: Xinxiang, Henan, and Shunyi, Beijing, during 2017 and 2018. Significant phenotypic and genetic variation for FER severity was observed, and FER resistance was significantly correlated among the four environments with a generalized heritability of 0.78. GWAS identified 23 SNPs that were associated with FER resistance, 2 of which (1_226233417 on chromosome 1 and 10_14501044 on chromosome 10) were associated at threshold of 2.65 × 10−7 [−log(0.01/37,801)]. Using BSA, resistance quantitative trait loci were identified on chromosomes 3, 4, 7, 9, and 10 at the 90% confidence level and on chromosomes 3 and 10 at the 95% confidence level. A key region, bin 10.03, was detected by both GWAS and BSA. Genomic prediction for FER resistance showed that the prediction accuracy by trait-related markers was higher than that by randomly selected markers under different levels of marker density. Marker-assisted selection using genomic prediction could be an efficient strategy for genetic improvement for complex traits like FER resistance.

scholarly journals The Effect of northern corn leaf blight severity on Fusarium ear rot incidence of maize

2020 ◽  
Vol 116 (11/12) ◽  
Author(s):  
Maryke Craven ◽  
Liesl Morey ◽  
Liesl Morey ◽  
Adrian Abrahams ◽  
Henry A. Njom ◽  
...  
Keyword(s):  
South Africa ◽  
Agricultural Research ◽  
Fusarium Verticillioides ◽  
Plant Stress ◽  
Leaf Blight ◽  
Fusarium Ear Rot ◽  
Ear Rot ◽  
Northern Corn Leaf Blight ◽  
Progress Curve ◽  
Corn Leaf Blight

Northern corn leaf blight (NCLB) caused by Exserohilum turcicum and Fusarium ear rot caused by Fusarium verticillioides, are economically important maize diseases in South Africa. The effect of induced plant stress by NCLB on F. verticillioides ear rot and fumonisin production is unknown. Four field trials were conducted during 2016/2017 and 2017/2018 (November and December planting dates) at the Agricultural Research Council – Grain Crops in Potchefstroom (South Africa). Three maize cultivars with varying resistance levels to NCLB were selected (IMP50-10B – susceptible, BG3292 – moderately susceptible, DKC 61-94BR – resistant). NCLB severities were created through eight treatments: TMT1 – maximum control (three fungicide applications); TMT2 – standard control (two fungicide applications) and TMT3 – natural control (not inoculated or sprayed). The remaining treatments were inoculated with a cocktail of five NCLB races (Race 3, 3N, 23, 23N and 13N): TMT4 (five weeks after planting / WAP); TMT5 (five and six WAP); TMT6 (five, six and seven WAP); TMT7 (six and seven WAP); and TMT8 (seven WAP). Maize ears were naturally infected with F. verticillioides. Fifteen random plants were labelled at dent stage and NCLB severity (%), area under the disease progress curve, ear rot diseased area, ear rot severity (%), ear rot incidence (%) and total fumonisins (FB1+FB2+FB3; ug/kg) were established. Low levels of cob rot severity and fumonisins were obtained in all four trials. NCLB severity did not affect ear rot related parameters measured. Mean fumonisin levels were below the South African tolerance levels. Fumonisin concentrations differed significantly between cultivars but was not affected by NCLB severity or the cultivar x treatment interaction.

Sign in / Sign up

Export Citation Format

Share Document