scholarly journals Induced Systemic Resistance in Arabidopsis thaliana in Response to Root Inoculation with Pseudomonas fluorescens CHA0

2003 ◽  
Vol 16 (10) ◽  
pp. 851-858 ◽  
Author(s):  
Annalisa Iavicoli ◽  
Emmanuel Boutet ◽  
Antony Buchala ◽  
Jean-Pierre Métraux
Keyword(s):  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Pseudomonas Fluorescens ◽  
Transgenic Line ◽  
Induced Systemic Resistance ◽  
Antibiotic Activity ◽  
Systemic Resistance ◽  
Peronospora Parasitica ◽  
Molecular Processes ◽  
Molecular Determinants

Root inoculation of Arabidopsis thaliana ecotype Columbia with Pseudomonas fluorescens CHA0r partially protected leaves from the oomycete Peronospora parasitica. The molecular determinants of Pseudomonas fluorescens CHA0r for this induced systemic resistance (ISR) were investigated, using mutants derived from strain CHA0: CHA400 (pyoverdine deficient), CHA805 (exoprotease deficient), CHA77 (HCN deficient), CHA660 (pyoluteorin deficient), CHA631 (2,4-diacetylphloroglucinol [DAPG] deficient), and CHA89 (HCN, DAPG- and pyoluteorin deficient). Only mutations interfering with DAPG production led to a significant decrease in ISR to Peronospora parasitica. Thus, DAPG production in Pseudomonas fluorescens is required for the induction of ISR to Peronospora parasitica. DAPG is known for its antibiotic activity; however, our data indicate that one action of DAPG could be due to an effect on the physiology of the plant. DAPG at 10 to 100 μM applied to roots of Arabidopsis mimicked the ISR effect. CHA0r-mediated ISR was also tested in various Arabidopsis mutants and transgenic plants: NahG (transgenic line degrading salicylic acid [SA]), sid2-1 (nonproducing SA), npr1-1 (non-expressing NPR1 protein), jar1-1 (insensitive to jasmonic acid and methyl jasmonic acid), ein2-1 (insensitive to ethylene), etr1-1 (insensitive to ethylene), eir1-1 (insensitive to ethylene in roots), and pad2-1 (phytoalexin deficient). Only jar1-1, eir1-1, and npr1-1 mutants were unable to undergo ISR. Sensitivity to jasmonic acid and functional NPR1 and EIR1 proteins were required for full expression of CHA0r-mediated ISR. The requirements for ISR observed in this study in Peronospora parasitica induced by Pseudomonas fluorescens CHA0r only partially overlap with those published so far for Peronospora parasitica, indicating a great degree of flexibility in the molecular processes leading to ISR.

scholarly journals Induced Systemic Resistance in Arabidopsis thaliana Against Pseudomonas syringae pv. tomato by 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens

2012 ◽  
Vol 102 (4) ◽  
pp. 403-412 ◽  
Author(s):  
David M. Weller ◽  
Dmitri V. Mavrodi ◽  
Johan A. van Pelt ◽  
Corné M. J. Pieterse ◽  
Leendert C. van Loon ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pseudomonas Fluorescens ◽  
Induced Resistance ◽  
Pseudomonas Syringae ◽  
Induced Systemic Resistance ◽  
Signal Transduction Pathway ◽  
Systemic Resistance ◽  
Wild Type ◽  
Challenge Inoculation ◽  
Dependent Signal

Pseudomonas fluorescens strains that produce the polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) are among the most effective rhizobacteria that suppress root and crown rots, wilts, and damping-off diseases of a variety of crops, and they play a key role in the natural suppressiveness of some soils to certain soilborne pathogens. Root colonization by 2,4-DAPG-producing P. fluorescens strains Pf-5 (genotype A), Q2-87 (genotype B), Q8r1-96 (genotype D), and HT5-1 (genotype N) produced induced systemic resistance (ISR) in Arabidopsis thaliana accession Col-0 against bacterial speck caused by P. syringae pv. tomato. The ISR-eliciting activity of the four bacterial genotypes was similar, and all genotypes were equivalent in activity to the well-characterized strain P. fluorescens WCS417r. The 2,4-DAPG biosynthetic locus consists of the genes phlHGF and phlACBDE. phlD or phlBC mutants of Q2-87 (2,4-DAPG minus) were significantly reduced in ISR activity, and genetic complementation of the mutants restored ISR activity back to wild-type levels. A phlF regulatory mutant (overproducer of 2,4-DAPG) had ISR activity equivalent to the wild-type Q2-87. Introduction of DAPG into soil at concentrations of 10 to 250 μM 4 days before challenge inoculation induced resistance equivalent to or better than the bacteria. Strain Q2-87 induced resistance on transgenic NahG plants but not on npr1-1, jar1, and etr1 Arabidopsis mutants. These results indicate that the antibiotic 2,4-DAPG is a major determinant of ISR in 2,4-DAPG-producing P. fluorescens, that the genotype of the strain does not affect its ISR activity, and that the activity induced by these bacteria operates through the ethylene- and jasmonic acid-dependent signal transduction pathway.

scholarly journals Transcript and metabolite analysis of the Trichoderma-induced systemic resistance response to Pseudomonas syringae in Arabidopsis thaliana

Microbiology ◽  
2012 ◽  
Vol 158 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Yariv Brotman ◽  
Jan Lisec ◽  
Michaël Méret ◽  
Ilan Chet ◽  
Lothar Willmitzer ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pseudomonas Syringae ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Metabolite Analysis ◽  
Resistance Response

scholarly journals Jasmonic Acid and Ethylene Signaling Pathways Regulate Glucosinolate Levels in Plants During Rhizobacteria-Induced Systemic Resistance Against a Leaf-Chewing Herbivore

2016 ◽  
Vol 42 (12) ◽  
pp. 1212-1225 ◽  
Author(s):  
Nurmi Pangesti ◽  
Michael Reichelt ◽  
Judith E. van de Mortel ◽  
Eleni Kapsomenou ◽  
Jonathan Gershenzon ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Signaling Pathways ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Ethylene Signaling

scholarly journals Induced systemic resistance in cucumber and Arabidopsis thaliana by the combination of Trichoderma harzianum Tr6 and Pseudomonas sp. Ps14

2013 ◽  
Vol 65 (1) ◽  
pp. 14-23 ◽  
Author(s):  
Hamidreza Alizadeh ◽  
Keivan Behboudi ◽  
Masoud Ahmadzadeh ◽  
Mohammad Javan-Nikkhah ◽  
Christos Zamioudis ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Trichoderma Harzianum ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Pseudomonas Sp

The discovery of new plant activators and scaffolds with potential induced systemic resistance: from jasmonic acid to pyrrolidone

MedChemComm ◽  
2016 ◽  
Vol 7 (9) ◽  
pp. 1849-1857 ◽  
Author(s):  
Kang Chang ◽  
Yanxia Shi ◽  
Jianqin Chen ◽  
Zenghui He ◽  
Zheng Xu ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Induced Systemic Resistance ◽  
Systemic Resistance

A series of novel plant activators possessing a pyrrolidone scaffold was developed with the help of SHAFTS.

Induced resistance and control of charcoal rot in Cicer arietinum (chickpea) by Pseudomonas fluorescens

2001 ◽  
Vol 79 (7) ◽  
pp. 787-795 ◽  
Author(s):  
Alok K Srivastava ◽  
Tanuja Singh ◽  
T K Jana ◽  
Dilip K Arora
Keyword(s):  
Pseudomonas Fluorescens ◽  
Cicer Arietinum ◽  
Induced Resistance ◽  
Time Course ◽  
Induced Systemic Resistance ◽  
Macrophomina Phaseolina ◽  
Systemic Resistance ◽  
Charcoal Rot ◽  
Chemical Inducers ◽  
Rot Disease

Pseudomonas fluorescens isolate 4-92 induced systemic resistance against charcoal rot disease in chickpea (Cicer arietinum L.) caused by Macrophomina phaseolina (Tassi) Goidanich. Time-course accumulation of pathogenesis-related (PR) proteins (chitinases and glucanases) in chickpea plants inoculated with P. fluorescens was significantly (P = 0.05) higher than in control plants. The level of chitinases and glucanases increased by 6.6- to 7-fold up to 4 days postinoculation; thereafter, little decrease in the activity of PR proteins was observed. Root-colonizing populations of P. fluorescens were at a maximum 2 days after transplantation at different inoculum concentrations, and decreased over time. Inoculation of root tips of chickpea by P. fluorescens, 2,6-dichloroisonicotinic acid, and o-acetylsalicylic acid induced systemic resistance against charcoal rot. Disease was 33 to 55.5% higher in control plants than in plants inoculated with chemical inducers or P. fluorescens. Single treatment of plants with P. fluorescens increased disease resistance by 33%, whereas combined application of P. fluorescens with either of the chemical inducers was most effective in inducing the resistance by 2- to 2.25-fold. The time-course study shows that an interval of at least 2 days was required between induction treatment and challenge inoculation. Biocontrol efficacy of P. fluorescens against charcoal rot disease in chickpea was demonstrated under greenhouse conditions.Key words: biological control, induced resistance, Macrophomina phaseolina, Pseudomonas fluorescens.

scholarly journals Simultaneous Interaction of Arabidopsis thaliana with Bradyrhizobium Sp. Strain ORS278 and Pseudomonas syringae pv. tomato DC3000 Leads to Complex Transcriptome Changes

2008 ◽  
Vol 21 (2) ◽  
pp. 244-259 ◽  
Author(s):  
Fabienne Cartieaux ◽  
Céline Contesto ◽  
Adrien Gallou ◽  
Guilhem Desbrosses ◽  
Joachim Kopka ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Pseudomonas Syringae ◽  
Plant Growth Promoting Rhizobacteria ◽  
Systemic Resistance ◽  
Bradyrhizobium Sp ◽  
Plant Growth Promoting ◽  
Induced Changes ◽  
Molecular Bases ◽  
First Time

Induced systemic resistance (ISR) is a process elicited by telluric microbes, referred to as plant growth-promoting rhizobacteria (PGPR), that protect the host plant against pathogen attacks. ISR has been defined from studies using Pseudomonas strains as the biocontrol agent. Here, we show for the first time that a photosynthetic Bradyrhizobium sp. strain, ORS278, also exhibits the ability to promote ISR in Arabidopsis thaliana, indicating that the ISR effect may be a widespread ability. To investigate the molecular bases of this response, we performed a transcriptome analysis designed to reveal the changes in gene expression induced by the PGPR, the pathogen alone, or by both. The results confirm the priming pattern of ISR described previously, meaning that a set of genes, of which the majority was predicted to be influenced by jasmonic acid or ethylene, was induced upon pathogen attack when plants were previously colonized by PGPR. The analysis and interpretation of transcriptome data revealed that 12-oxo-phytodienoic acid, an intermediate of the jasmonic acid biosynthesis pathway, is likely to be an actor in the signaling cascade involved in ISR. In addition, we show that the PGPR counterbalanced the pathogen-induced changes in expression of a series of genes.

scholarly journals Extracts from cultures of Pseudomonas fluorescens induce defensive patterns of gene expression and enzyme activity while depressing visible injury and reactive oxygen species in Arabidopsis thaliana challenged with pathogenic Pseudomonas syringae

AoB Plants ◽  
2019 ◽  
Vol 11 (5) ◽  
Author(s):  
H Martin-Rivilla ◽  
A Garcia-Villaraco ◽  
B Ramos-Solano ◽  
F J Gutierrez-Mañero ◽  
J A Lucas
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
Pseudomonas Fluorescens ◽  
Pseudomonas Syringae ◽  
Reactive Oxygen ◽  
Signalling Pathway ◽  
Systemic Resistance ◽  
Oxygen Species ◽  
Ros Scavenging

Abstract We evaluated the ability of metabolic elicitors extracted from Pseudomonas fluorescens N21.4 to induce systemic resistance (ISR) in Arabidopsis thaliana against the pathogen Pseudomonas syringae DC3000. Metabolic elicitors were obtained from bacteria-free culture medium with n-hexane, ethyl acetate and n-butanol in three consecutive extractions. Each extract showed plant protection activity. The n-hexane fraction was the most effective and was used to study the signal transduction pathways involved by evaluating expression of marker genes of the salicylic acid (SA) signalling pathway (NPR1, PR1, ICS and PR2) and the jasmonic acid/ethylene (JA/ET) signalling pathway (PDF1, MYC2, LOX2 and PR3). In addition, the level of oxidative stress was tested by determining the activity of enzymes related to the ascorbate-glutathione cycle. N-hexane extracts stimulated both pathways based on overexpression of ICS, PR1, PR2, PDF1 and LOX2 genes. In addition, activity of the pathogenesis-related proteins glucanase (PR2) and chitinase (PR3), lipoxygenase and polyphenol oxidase was enhanced together with an increased capacity to remove reactive oxygen species (ROS). This was associated with less oxidative stress as indicated by a decrease in malondialdehyde (MDA), suggesting a causative link between defensive metabolism against P. syringae and ROS scavenging.

Sign in / Sign up

Export Citation Format

Share Document