scholarly journals A Survey of Viruses Affecting French bean (Phaseolus vulgaris) in Iran Includes a First Report of Southern bean mosaic virus and Bean pod mottle virus

Plant Disease ◽  
2005 ◽  
Vol 89 (9) ◽  
pp. 1012-1012 ◽  
Author(s):  
N. Shahraeen ◽  
T. Ghotbi ◽  
A. Dezaje Elkhache ◽  
A. Sahandi
Keyword(s):  
Phaseolus Vulgaris ◽  
Mosaic Virus ◽  
Plant Viruses ◽  
French Bean ◽  
Mottle Virus ◽  
Bean Pod Mottle Virus ◽  
Southern Bean Mosaic Virus ◽  
Leaf Distortion ◽  
Test Plants ◽  
Das Elisa

A survey was conducted from 2003 to 2004 to identify viruses infecting common bean (Phaseolus vulgaris L.) in different growing areas of East Azarbaejan Province of Iran. A total of 300 French bean samples with symptoms of viral infection (mosaic, vein clearing, leaf rolling, yellowing, and leaf distortion) were collected. The samples were tested for eight viruses using the tissue-blot immunoassay procedures (TBIA) (2) and double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) according to the manufacturer's instructions (DSMZ, Braun-schweig, Germany). ELISA tests for Alfalfa mosaic virus (AMV), Bean yellow mosaic virus (BYMV), Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), Cucumber mosaic virus (CMV), Bean leaf roll virus (BLRV), and Southern bean mosaic virus (SBMV) were used. In addition, antiserum was provided by S. A. Ghabrial (University of Kentucky, Lexington) to test for Bean pod mottle virus (BPMV). Serological tests showed that SBMV and BPMV were present in 12% (35 samples) and 5% (15 samples) of samples, respectively. BCMV, BCMNV, BYMV, BLRV, CMV, and AMV were more common and were detected in 155, 105, 80, 46, 30, and 10 samples of 300 samples, respectively. These six viruses were previously reported in other pulses and in French bean in Iran (1). The presence of SBMV and BPMV were verified in samples by transmission to French bean (Phaseolus vulgaris), cowpea (Vigna unguiculata), and soybean (Glycine max) indicator test plants (3,4). Inoculation with extracts from SBMV-positive plants produced systemic mottle and mosaic symptoms in soybean (cv. Gorgan-3) and French bean (cvs. Dubbele Witte and Cheete). In cowpea (cv. Mashad) and French bean (cv. Pinto), inoculation produced necrotic local lesions. Inoculation with extracts from BPMV-positive plants produced severe mosaic, leaf distortion, and puckering in soybean (cv. Gorgan-3) and French bean (cv. Ten-dergreen). No symptoms were observed in cowpea (cv. Mashad). Cvs. Pinto and Bountiful bean reacted with necrotic local lesions. All indicator test plants tested positive for the presence of SBMV or BPMV as expected using DAS-ELISA. To our knowledge, this is the first report of BPMV and SBMV naturally infecting French bean in Iran. These viruses can cause a serious problem to other leguminous crops grown in Iran. References: (1) W. J. Kaiser et al. Plant Dis. Rep. 52:687, 1968. (2) K. M. Makkouk and A. Comeau. Eur. J. Plant Pathol. 100:71, 1994. (3) J. S. Semancik. Bean pod mottle virus. No. 108 in: Descriptions of Plant Viruses. CMI/AAB, Kew, Surrey, England, 1972. (4) J. H. Tremain and R. I. Hamilton. Southern bean mosaic virus. No. 274 in: Descriptions of Plant Viruses. CMI/AAB, Kew, Surrey, England, 1983.

scholarly journals Structural Fingerprinting: Subgrouping of Comoviruses by Structural Studies of Red Clover Mottle Virus to 2.4-Å Resolution and Comparisons with Other Comoviruses

2000 ◽  
Vol 74 (1) ◽  
pp. 493-504 ◽  
Author(s):  
Tianwei Lin ◽  
Anthony J. Clark ◽  
Zhongguo Chen ◽  
Michael Shanks ◽  
Jin-Bi Dai ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Red Clover ◽  
Plant Viruses ◽  
Small Subunit ◽  
Mottle Virus ◽  
Coat Proteins ◽  
Structural Studies ◽  
Bean Pod Mottle Virus ◽  
X Ray ◽  
N Terminus

ABSTRACT Red clover mottle virus (RCMV) is a member of the comoviruses, a group of picornavirus-like plant viruses. The X-ray structure of RCMV strain S has been determined and refined to 2.4 Å. The overall structure of RCMV is similar to that of two other comoviruses, Cowpea mosaic virus (CPMV) and Bean pod mottle virus (BPMV). The sequence of the coat proteins of RCMV strain O were modeled into the capsid structure of strain S without causing any distortion, confirming the close resemblance between the two strains. By comparing the RCMV structure with that of other comoviruses, a structural fingerprint at the N terminus of the small subunit was identified which allowed subgrouping of comoviruses into CPMV-like and BPMV-like viruses.

scholarly journals DOENÇAS CAUSADAS POR VÍRUS NA CULTURA DE FEIJOEIRO (Phaseolus vulgaris L.)

2010 ◽  
Vol 3 (2) ◽  
pp. 1-6 ◽  
Author(s):  
Felipe Rafael Garcés Fiallos
Keyword(s):  
Phaseolus Vulgaris ◽  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Mottle Virus ◽  
Phaseolus Vulgaris L ◽  
Bean Golden Mosaic Virus ◽  
Southern Bean Mosaic Virus ◽  
Rugose Mosaic

O feijoeiro é atacado, além de fungos, nematóides e bactérias, por vírus que podem causar danos e perdas numa lavoura. As doenças causadas por vírus têm exercido um papel relevante na baixa produtividade do feijoeiro no Brasil e outros países Latino-Americanos. Os métodos de controle para as viroses de plantas cultivadas são, em geral, muito restritos. Pela falta desta informação e pela importância da cultura do feijão, se traz esta revisão, onde são relatadas as doenças virais mais importantes desta cultura dentro do Brasil e fora dele, explicando detalhadamente sua sintomatologia, etiologia e seu controle. As doenças viróticas mais importantes no feijoeiro no Brasil são o bean common mosaic virus (BCMV), bean golden mosaic virus (BGMV), bean rugose mosaic virus (BRMV), cowpea mild mottle virus (CpMMV) e southern bean mosaic virus (SBMV). Esta revisão traz o mais importante em relação às doenças primordiais causadas por vírus de plantas na cultura de feijão.

scholarly journals First Report of Southern bean mosaic virus Infecting French Bean in Morocco

Plant Disease ◽  
2004 ◽  
Vol 88 (10) ◽  
pp. 1162-1162 ◽  
Author(s):  
E. Segundo ◽  
F. M. Gil-Salas ◽  
D. Janssen ◽  
G. Martin ◽  
I. M. Cuadrado ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Sodium Dodecyl ◽  
Plant Viruses ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Sequence Identity ◽  
Southern Bean Mosaic Virus ◽  
Bean Plants ◽  
Symptomatic Leaf ◽  
Das Elisa

Common bean (Phaseolus vulgaris L.) is grown on approximately 1,500 ha in commercial greenhouses and is of major economic importance in the Souss-Massa Region, Agadir, Morocco. Since October 2003, symptoms resembling a viral disease, consisting of pod mosaic and distortion and mild to severe mosaic in leaves, have been observed on bean plants in several greenhouses. Mechanical inoculation with symptomatic leaf extracts produced necrotic local lesions on P. vulgaris ‘Pinto’ and systemic symptoms similar to those observed in the naturally infected bean plants P. vulgaris ‘Donna’ (five plants per cultivar). Inoculated and naturally infected samples reacted positively using a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) to Southern bean mosaic virus (SBMV) (DSMZ, Braunschweig, Germany), a member of the Sobemovirus genus that is transmitted by contact, soil, beetles, and seeds (1). Virions purified from a naturally infected ‘Donna’ plant contained a 30-kDa polypeptide that reacted positively using sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blot analysis with SBMV antiserum (DSMZ). Reverse transcription-polymerase chain reaction amplification with SMBV primers as described by Verhoeven et al. (2) produced an expected 870-bp band. The amplicon was cloned, sequenced (GenBank Accession No. AJ748276), and compared to those isolates available in GenBank and had a nucleotide sequence identity of 87% and a derived amino acid sequence identity of 95% with an SBMV isolate from Spain (2). During a survey in different areas of the Souss-Massa Region, 20 symptomatic leaf and pod samples were randomly collected from 12 greenhouses (50 ha) where significant commercial losses were suffered because of this virus disease, and all samples were positive using DAS-ELISA for SBMV. To our knowledge, this is the first report of SBMV in Morocco. References: (1) J. H. Tremaine and R. I. Hamilton. Southern bean mosaic virus. No. 274 in: Descriptions of Plant Viruses. CMI/AAB, Kew, Surrey, England, 1983. (2) J. Th. J. Verhoeven et al. Eur. J. Plant Pathol. 109:935, 2003.

scholarly journals Análise molecular de segmento do RNA2 de comovirus isolados de soja no estado do Paraná

2002 ◽  
Vol 27 (5) ◽  
pp. 525-527 ◽  
Author(s):  
ELIEZER R. SOUTO ◽  
ÁLVARO M. R. ALMEIDA ◽  
ANÉSIO BIANCHINI ◽  
FÁBIO SARTORI ◽  
ÉBERSON S. CALVO
Keyword(s):  
Glycine Max ◽  
Phaseolus Vulgaris ◽  
Mosaic Virus ◽  
Mottle Virus ◽  
Bean Pod Mottle Virus ◽  
Bean Golden Mosaic Virus ◽  
Rugose Mosaic

Nas áreas produtoras de feijão (Phaseolus vulgaris) do Estado do Paraná observa-se anualmente a ocorrência do vírus do mosaico em desenho do feijoeiro (Bean rugose mosaic virus, BRMV), principalmente em infecções mistas com o vírus do mosaico dourado do feijoeiro (Bean golden mosaic virus, BGMV), acarretando maior severidade de sintomas e causando perdas na produção. Recentemente constatou-se a presença do vírus do mosaico severo do caupi (Cowpea severe mosaic virus, CPSMV) associado a sintomas de queima do broto em plantações de soja (Glycine max) na região de Londrina, sendo este um fato novo no Estado. Neste trabalho, parte do RNA2 de dois comovirus isolados de soja no Paraná foram clonados e sequenciados, sendo 600 pares de bases (pb) do BRMV-PR e 594 pb do CPSMV-PR. Posteriormente, as seqüências correspondentes de aminoácidos foram comparadas com seis seqüências de vírus do gênero Comovirus depositadas no GenBank. Com base nestes dados observou-se que o segmento do RNA2 do isolado CPSMV-PR apresentou homologia de 85% com parte de uma seqüência já conhecida do RNA2 do CPSMV, enquanto que o segmento do RNA2 do isolado BRMV-PR apresentou homologia de 39% com o CPSMV, e de 44% com o Bean pod mottle virus (BPMV). Este trabalho apresenta pela primeira vez dados de sequenciamento parcial do BRMV, o que poderá contribuir para sua completa caracterização molecular e para o estabelecimento de estratégias para obtenção de plantas resistentes ao vírus.

scholarly journals DOENÇAS CAUSADAS POR VÍRUS NA CULTURA DE FEIJOEIRO (Phaseolus vulgaris L.)

2010 ◽  
Vol 3 (2) ◽  
pp. 1-6
Author(s):  
Felipe Rafael Garcés Fiallos
Keyword(s):  
Phaseolus Vulgaris ◽  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Mottle Virus ◽  
Phaseolus Vulgaris L ◽  
Bean Golden Mosaic Virus ◽  
Southern Bean Mosaic Virus ◽  
Rugose Mosaic

O feijoeiro é atacado, além de fungos, nematóides e bactérias, por vírus que podem causar danos e perdas numa lavoura. As doenças causadas por vírus têm exercido um papel relevante na baixa produtividade do feijoeiro no Brasil e outros países Latino-Americanos. Os métodos de controle para as viroses de plantas cultivadas são, em geral, muito restritos. Pela falta desta informação e pela importância da cultura do feijão, se traz esta revisão, onde são relatadas as doenças virais mais importantes desta cultura dentro do Brasil e fora dele, explicando detalhadamente sua sintomatologia, etiologia e seu controle. As doenças viróticas mais importantes no feijoeiro no Brasil são o bean common mosaic virus (BCMV), bean golden mosaic virus (BGMV), bean rugose mosaic virus (BRMV), cowpea mild mottle virus (CpMMV) e southern bean mosaic virus (SBMV). Esta revisão traz o mais importante em relação às doenças primordiais causadas por vírus de plantas na cultura de feijão.

scholarly journals First Report of Cucumber mosaic virus in Banana from Iran

Plant Disease ◽  
2005 ◽  
Vol 89 (8) ◽  
pp. 914-914 ◽  
Author(s):  
T. Ghotbi ◽  
K. Bananej
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Viruses ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Serological Tests ◽  
Tropical Fruit ◽  
Test Plants ◽  
Das Elisa

Banana bunchy top virus (BBTV), Banana streak virus (BSV), and Cucumber mosaic virus (CMV) (genus Cucumovirus, family Bromoviridae [2]) cause widespread economic losses on banana (Musa sp.) throughout the world and have been reported on banana in different countries including Pakistan along its southeastern border with Iran (1). A survey was conducted from 2004–2005 to identify viruses infecting banana in greenhouses in different growing areas in northern Iran, Mazandaran Province (Sari, Babol, Behshahr, and Ghaemshahr cities). A total of 180 samples from seven banana-growing greenhouses with symptoms of mosaic, chlorosis, stunting, and fruit malformation were collected. All samples were tested for CMV with polyclonal antibodies using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) (CMV strain D subgroup I; gifted by H. Lecoq, INRA, Avignion, France). For sap inoculation onto indicator test plants, selected ELISA-positive leaf samples were ground in chilled 0.01 M phosphate buffer, pH 7.0, containing 0.15% 2-mercaptoethanol. Chlorotic and necrotic local lesions developed on Chenopodium amaranticolor and Vigna unguiculata (cv. Mashad local) 10 and 12 days postinoculation, respectively. Cucumis sativus and Nicotiana rustica also developed systemic mosaic symptoms (3). All indicator test plants were rechecked for the presence of CMV using DAS-ELISA. On the basis of serological tests and indicator host plants reactions, CMV was identified in 32% of samples including Sari (13.8%), Babol (2.7%), Behshahr (10%) and Ghahemshahr (5%), respectively. Fifty-five samples did not react with CMV antiserum but the presence of symptoms resembling BBTV and BSV (4) emphasizes the need for further investigations to confirm the presence and identities of other viruses. References: (1) J. Bird and F. L. Wellman. Phytopathology 52:286, 1962. (2) S. K. Choi et al. J. Virol. Methods 83:67, 1999. (3) A. J. Gibbs and B. D. Harrison. Descriptions of Plant Viruses. No.1. CMI/AAB, Surrey, England, 1970. (4) R. C. Ploetz et al., eds. Compendium of Tropical Fruit Diseases. The American Phytopathological Society, St. Paul, MN, 1994.

scholarly journals DOENÇAS CAUSADAS POR VÍRUS NA CULTURA DE FEIJOEIRO (Phaseolus vulgaris L.)

2010 ◽  
Vol 3 (2) ◽  
pp. 1
Author(s):  
Felipe Rafael Garcés Fiallos
Keyword(s):  
Phaseolus Vulgaris ◽  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Mottle Virus ◽  
Phaseolus Vulgaris L ◽  
Bean Golden Mosaic Virus ◽  
Southern Bean Mosaic Virus ◽  
Rugose Mosaic

O feijoeiro é atacado, além de fungos, nematóides e bactérias, por vírus que podem causar danos e perdas numa lavoura. As doenças causadas por vírus têm exercido um papel relevante na baixa produtividade do feijoeiro no Brasil e outros países Latino-Americanos. Os métodos de controle para as viroses de plantas cultivadas são, em geral, muito restritos. Pela falta desta informação e pela importância da cultura do feijão, se traz esta revisão, onde são relatadas as doenças virais mais importantes desta cultura dentro do Brasil e fora dele, explicando detalhadamente sua sintomatologia, etiologia e seu controle. As doenças viróticas mais importantes no feijoeiro no Brasil são o bean common mosaic virus (BCMV), bean golden mosaic virus (BGMV), bean rugose mosaic virus (BRMV), cowpea mild mottle virus (CpMMV) e southern bean mosaic virus (SBMV). Esta revisão traz o mais importante em relação às doenças primordiais causadas por vírus de plantas na cultura de feijão.

scholarly journals First Report of Japanese Beetle (Popillia japonica) as a Vector of Southern bean mosaic virus and Bean pod mottle virus

Plant Disease ◽  
2007 ◽  
Vol 91 (5) ◽  
pp. 637-637 ◽  
Author(s):  
S. L. Wickizer ◽  
R. C. Gergerich
Keyword(s):  
United States ◽  
The United States ◽  
Plant Viruses ◽  
Popillia Japonica ◽  
Feeding Damage ◽  
Japanese Beetle ◽  
Bean Pod Mottle Virus ◽  
Southern Bean Mosaic Virus ◽  
Bait Plants ◽  
Test Plants

The Japanese beetle, Popillia japonica (Coleoptera: Scarabaeidae), was introduced into the United States in New Jersey in 1916 and now commonly occurs in the eastern and mid-western United States. The Japanese beetle has a wide host range and feeds on more than 300 plant species within 79 families (2), but it has not been reported to be a vector of plant viruses. Southern bean mosaic virus (SBMV; genus Sobemovirus) and Bean pod mottle virus (BPMV; family Comoviridae, genus Comovirus) are transmitted by several species of leaf-feeding beetles in the families Coccinelidae and Chrysomelidae (1). Japanese beetles, collected in northwestern Arkansas with a JB Jumbo insect trap (Great Lakes IPM, Inc., Vestaburg, MI), were tested to determine if they are able to transmit SBMV and BPMV. The beetles were given a 24-h acquisition access (individually in plastic petri dishes) to detached trifoliolate leaves of Phaseolus vulgaris ‘Black Valentine’ systemically infected with either BPMV or SBMV. Beetles that had fed on the infected bean leaves (as evidenced by feeding damage to the leaves) were caged individually in clear plastic cups on healthy ‘Black Valentine’ bean seedlings for a 24-h transmission access period. The test plants were grown in the greenhouse for 2 weeks after which they were evaluated for virus infection. In the first trial, BPMV was not transmitted (0 of 11 bait plants with feeding damage), whereas SBMV was efficiently transmitted (6 of 6 bait plants with feeding damage) as determined by agar-gel double-diffusion serology of test plants using polyclonal antibodies to SBMV and BPMV. In a second trial, with a different set of beetles, the transmission rate for BPMV was 3 of 46 (6.5%) and 33 of 43 (77%) for SBMV, and the test plants were evaluated for virus infection using Protein A ELISA. The higher transmission efficiency of SBMV by Japanese beetles when compared with BPMV is characteristic of these two viruses when they are transmitted by other leaffeeding beetles (1). These results are in contrast to an earlier report that Japanese beetles were not able to transmit BPMV from infected soybeans to healthy soybeans in Illinois (3). To our knowledge, this is the first report that the Japanese beetle, an introduced insect with a wide host range in the United States, is a vector of plant viruses. References: (1) J. P. Fulton et al. Ann. Rev. Phytopathol. 25:111. 1987. (2) D. W. Held. J. Aboricult. 30:328, 2004. (3) T. R. Mabry et al. Plant Dis. 87:1221, 2003.

scholarly journals R-BPMV-Mediated Resistance to Bean pod mottle virus in Phaseolus vulgaris L. Is Heat-Stable but Elevated Temperatures Boost Viral Infection in Susceptible Genotypes

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1239
Author(s):  
Chouaïb Meziadi ◽  
Julie Lintz ◽  
Masoud Naderpour ◽  
Charlotte Gautier ◽  
Sophie Blanchet ◽  
...  
Keyword(s):  
Elevated Temperature ◽  
Phaseolus Vulgaris ◽  
Elevated Temperatures ◽  
Heat Waves ◽  
Dominant Gene ◽  
Atmospheric Temperature ◽  
Mottle Virus ◽  
Bean Pod Mottle Virus ◽  
Heat Stable ◽  
The Impact

In the context of climate change, elevated temperature is a major concern due to the impact on plant–pathogen interactions. Although atmospheric temperature is predicted to increase in the next century, heat waves during summer seasons have already become a current problem. Elevated temperatures strongly influence plant–virus interactions, the most drastic effect being a breakdown of plant viral resistance conferred by some major resistance genes. In this work, we focused on the R-BPMV gene, a major resistance gene against Bean pod mottle virus in Phaseolus vulgaris. We inoculated different BPMV constructs in order to study the behavior of the R-BPMV-mediated resistance at normal (20 °C) and elevated temperatures (constant 25, 30, and 35 °C). Our results show that R-BPMV mediates a temperature-dependent phenotype of resistance from hypersensitive reaction at 20 °C to chlorotic lesions at 35 °C in the resistant genotype BAT93. BPMV is detected in inoculated leaves but not in systemic ones, suggesting that the resistance remains heat-stable up to 35 °C. R-BPMV segregates as an incompletely dominant gene in an F2 population. We also investigated the impact of elevated temperature on BPMV infection in susceptible genotypes, and our results reveal that elevated temperatures boost BPMV infection both locally and systemically in susceptible genotypes.

scholarly journals Detection and molecular characterization of Pepper mild mottle virus in Serbia

Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 651-663 ◽  
Author(s):  
Dragana Milosevic ◽  
Ivana Stankovic ◽  
Aleksandra Bulajic ◽  
Maja Ignjatov ◽  
Zorica Nikolic ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Great Influence ◽  
Alfalfa Mosaic Virus ◽  
Resistance Breeding ◽  
Elisa Test ◽  
Mottle Virus ◽  
Pepper Mild Mottle Virus ◽  
Das Elisa

During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P1,2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.

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