scholarly journals Characterization of Species of Diaporthe from Wood Cankers of Grape in Eastern North American Vineyards

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 912-920 ◽  
Author(s):  
Kendra Baumgartner ◽  
Phillip T. Fujiyoshi ◽  
Renaud Travadon ◽  
Lisa A. Castlebury ◽  
Wayne F. Wilcox ◽  
...  
Keyword(s):  
North America ◽  
Dna Sequences ◽  
Leaf Spot ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Eastern North America ◽  
Type Specimens ◽  
Internal Transcribed Spacer Region ◽  
Phomopsis Viticola ◽  
History Of

In eastern North America, Phomopsis cane and leaf spot, caused by Phomopsis viticola, is a foliar disease of grape but, in the Mediterranean climate of western North America, P. viticola is primarily associated with wood cankers, along with other Diaporthe spp. To determine the identity of wood-infecting Diaporthe spp. in eastern North America, 65 isolates were cultured from 190 wood-canker samples from 23 vineyards with a history of Phomopsis cane and leaf spot. Identification of 29 representative isolates was based initially on morphology, followed by phylogenetic analyses of DNA sequences of the ribosomal DNA internal transcribed spacer region, elongation factor subunit 1-α, and actin in comparison with those of type specimens. Three species were identified: P. viticola, P. fukushii, and Diaporthe eres. Inoculations onto woody stems of potted Vitis labruscana ‘Concord’ and V. vinifera ‘Chardonnay’ showed that D. eres and P. fukushii were pathogenic (mean lesion lengths of 7.4 and 7.1 mm, respectively, compared with 3.5 mm for noninoculated controls) but significantly less so than wood-canker and leaf-spot isolates of P. viticola (13.5 mm). All three species infected pruning wounds of Concord and Chardonnay in the field. Our finding of pathogenic, wood-infecting Diaporthe spp. in all 23 vineyards suggests a frequent co-occurrence of the foliar symptoms of Phomopsis cane and leaf spot and wood cankers, although the latter are not always due to P. viticola.

scholarly journals Phomopsis Stem Canker: A Reemerging Threat to Sunflower (Helianthus annuus) in the United States

2015 ◽  
Vol 105 (7) ◽  
pp. 990-997 ◽  
Author(s):  
Febina M. Mathew ◽  
Kholoud M. Alananbeh ◽  
James G. Jordahl ◽  
Scott M. Meyer ◽  
Lisa A. Castlebury ◽  
...  
Keyword(s):  
United States ◽  
Helianthus Annuus ◽  
Great Plains ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Stem Canker ◽  
The United States ◽  
Northern Great Plains ◽  
Type Specimens ◽  
Internal Transcribed Spacer Region

Phomopsis stem canker causes yield reductions on sunflower (Helianthus annuus L.) on several continents, including Australia, Europe, and North America. In the United States, Phomopsis stem canker incidence has increased 16-fold in the Northern Great Plains between 2001 and 2012. Although Diaporthe helianthi was assumed to be the sole causal agent in the United States, a newly described species, D. gulyae, was found to be the primary cause of Phomopsis stem canker in Australia. To determine the identity of Diaporthe spp. causing Phomopsis stem canker in the Northern Great Plains, 275 infected stems were collected between 2010 and 2012. Phylogenetic analyses of sequences of the ribosomal DNA internal transcribed spacer region, elongation factor subunit 1-α, and actin gene regions of representative isolates, in comparison with those of type specimens, confirmed two species (D. helianthi and D. gulyae) in the United States. Differences in aggressiveness between the two species were determined using the stem-wound method in the greenhouse; overall, D. helianthi and D. gulyae did not vary significantly (P ≤ 0.05) in their aggressiveness at 10 and 14 days after inoculation. These findings indicate that both Diaporthe spp. have emerged as sunflower pathogens in the United States, and have implications on the management of this disease.

scholarly journals Cryptic species of Curvularia in the culture collection of the Queensland Plant Pathology Herbarium

MycoKeys ◽  
2018 ◽  
Vol 35 ◽  
pp. 1-25 ◽  
Author(s):  
Yu Pei Tan ◽  
Pedro W. Crous ◽  
Roger G. Shivas
Keyword(s):  
Plant Pathology ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Culture Collection ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Air Sample ◽  
Elongation Factor 1

Several unidentified specimens of Curvularia deposited in the Queensland Plant Pathology Herbarium were re-examined. Phylogenetic analyses based on sequence data of the internal transcribed spacer region, partial fragments of the glyceraldehyde-3-phosphate dehydrogenase and the translation elongation factor 1-α genes, supported the introduction of 13 novel Curvularia species. Eight of the species described, namely, C.beasleyi sp. nov., C.beerburrumensis sp. nov., C.eragrosticola sp. nov., C.kenpeggii sp. nov., C.mebaldsii sp. nov., C.petersonii sp. nov., C.platzii sp. nov. and C.warraberensis sp. nov., were isolated from grasses (Poaceae) exotic to Australia. Only two species, C.lamingtonensis sp. nov. and C.sporobolicola sp. nov., were described from native Australian grasses. Two species were described from hosts in other families, namely, C.coatesiae sp. nov. from Litchichinensis (Sapindaceae) and C.colbranii sp. nov. from Crinumzeylanicum (Amaryllidaceae). Curvulariareesii sp. nov. was described from an isolate obtained from an air sample. Furthermore, DNA sequences from ex-type cultures supported the generic placement of C.neoindica and the transfer of Drechsleraboeremae to Curvularia.

scholarly journals Botryosphaeriaceae Species Associated With Cankers and Dieback Symptoms of Acacia mangium and Pinus caribaea var. hondurensis in Venezuela

Plant Disease ◽  
2016 ◽  
Vol 100 (12) ◽  
pp. 2455-2464 ◽  
Author(s):  
J. R. Úrbez-Torres ◽  
F. Castro-Medina ◽  
S. R. Mohali ◽  
W. D. Gubler
Keyword(s):  
Dna Sequences ◽  
Tree Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Acacia Mangium ◽  
Forest Tree ◽  
Pinus Caribaea ◽  
Internal Transcribed Spacer Region ◽  
Forest Tree Species ◽  
Wide Range

Several species in the Botryosphaeriaceae family cause wood stain, cankers, and dieback of trunks and branches in a wide range of forest tree species. The aim of this study was to characterize the botryosphaeriaceous fungi associated with decline symptoms observed in Acacia mangium and Pinus caribaea var. hondurensis, two economically important forest tree species grown in commercial plantations in Venezuela. Fungi isolated from symptomatic samples collected from both hosts in commercial sites were identified based on their morphology and DNA sequences of the internal transcribed spacer region (ITS1-5.8S-ITS2) and part of the β-tubulin and translation elongation factor 1-α genes. Lasiodiplodia theobromae and L. venezuelensis were routinely isolated from A. mangium and P. caribaea var. hondurensis. Additionally, the novel species Diplodia guayanensis was isolated and characterized from symptomatic and asymptomatic tissues of A. mangium. Multigene phylogenetic analyses along with restriction fragment length polymorphism studies further supported the identification of these species. A pathogenicity study was conducted under natural conditions and 12 weeks after inoculation all Botryosphaeriaceae spp. were shown to be highly virulent on A. mangium. Contrary, no lesions were observed in the wood of P. caribaea var. hondurensis when inoculated with L. theobromae and L. venezuelensis. However, both species were consistently reisolated from the asymptomatic tissue beyond the inoculation point. This study contributes to a better understand the role that species in the Botryosphaeriaceae play on disease symptoms and dieback of A. mangium and P. caribaea var. hondurensis from plantations in eastern Venezuela.

Alternaria guilanica sp. nov., a new fungal pathogen causing leaf spot and blight on eggplant in Iran

Phytotaxa ◽  
2021 ◽  
Vol 520 (2) ◽  
pp. 184-194
Author(s):  
ALIREZA POURSAFAR ◽  
ESMAEIL HASHEMLOU ◽  
YOUBERT GHOSTA ◽  
FATEMEH SALIMI ◽  
MOHAMMAD JAVAN-NIKKHAH
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Leaf Spot ◽  
Phylogenetic Analyses ◽  
Solanum Melongena ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Major Allergen ◽  
Pathogenicity Test ◽  
Internal Transcribed Spacer Region

Eggplant (Solanum melongena L.) is an economically important solanaceous crop in Iran with fruits used for food and traditional medicine. Despite the importance of Alternaria leaf spot and blight disease of solanaceous crops which is commonly seen in the fields, our knowledge about the causal agents on eggplant is limited. In this study, a set of large-spored Alternaria isolates was recovered from eggplant with leaf spot and blight symptoms in Somehsara region, Guilan province, Iran. All recovered isolates shared conspicuous morphological characteristics e.g. production of large, solitary conidia with several transverse disto- and eusepta and long tapering filamentous beak resemble those seen in the members of Alternaria section Porri. Multi-locus phylogenetic analyses based on the internal transcribed spacer region of nrDNA (ITS-rDNA) and parts of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), second largest subunit of RNA Polymerase II (RPB2), translation elongation factor 1-alpha (TEF1-α) and Alternaria major allergen (Alt a 1) gene sequences provided further evidence supporting not only their exact placement in Alternaria sect. Porri, but also in a distinct lineage representing a new species. The new species was named, described and illustrated herein as Alternaria guilanica sp. nov.. The phylogenetic and morphological comaprisions of the new species with other closely related species were also provided. Pathogenicity test conducted for the new strains revealed that they were capable to induce disease symptoms on eggplant leaves under greenhouse conditions, and re-isolation of the inoculated isolates confirmed Koch’s postulates.

Buchwaldoboletus xylophilus and Phlebopus portentosus, two non-ectomycorrhizal boletes from tropical China

Phytotaxa ◽  
2021 ◽  
Vol 520 (2) ◽  
pp. 137-154
Author(s):  
HUI-JING XIE ◽  
CHUN-XIA ZHANG ◽  
MING-XIA HE ◽  
ZHI-QUN LIANG ◽  
XIAO-HUA DENG ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Internal Transcribed Spacer Region ◽  
Tropical Asia ◽  
Line Drawings ◽  
Molecular Phylogenetic ◽  
Microscopic Features ◽  
Tropical China

Buchwaldoboletus xylophilus and Phlebopus portentosus (Boletales) are two non-ectomycorrhizal boletes, which are widely reported in tropical Asia. However, there is little information available about the taxonomy of these two boletes. Here, B. xylophilus and P. portentosus are redescribed based on morphology and molecular phylogenetic analyses of DNA sequences from nuclear 28S rDNA (28S), nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), translation elongation factor 1-α gene (TEF1) and the RNA polymerase II second largest subunit gene (RPB2). Detailed descriptions, color photos of fresh basidiomata, and line-drawings of microscopic features of the two taxa are presented.

Species relationships in the dasyurid marsupial genus Pseudantechinus (Marsupialia : Dasyuridae): a re-examination of the taxonomic status of Pseudantechinus roryi

2017 ◽  
Vol 65 (4) ◽  
pp. 240 ◽  
Author(s):  
L. S. Umbrello ◽  
P. A. Woolley ◽  
M. Westerman
Keyword(s):  
Dna Sequences ◽  
Isolation By Distance ◽  
Phylogenetic Analyses ◽  
Mitochondrial Gene ◽  
Taxonomic Status ◽  
Museum Collections ◽  
Species Relationships ◽  
Type Specimens ◽  
Network Analyses ◽  
The Status

The status of Pseudantechinus roryi relative to its congeners has been determined from DNA sequences obtained from both nuclear and mitochondrial gene loci. Although all other recognised species of Pseudantechinus form reciprocally monophyletic lineages in phylogenetic analyses, individuals identified in museum collections as Ps. roryi (including type specimens) were indistinguishable from those identified as Ps. macdonnellensis. Ps. roryi is thus considered to be a synonym of Ps. macdonnellensis. Neighbour-joining network analyses failed to reveal any clear biogeographic differences between populations of Ps. macdonnellensis other than some evidence of isolation by distance.

scholarly journals Six new species and reports of Hydnum (Cantharellales) from eastern North America

MycoKeys ◽  
2018 ◽  
Vol 42 ◽  
pp. 35-72 ◽  
Author(s):  
Rachel A. Swenie ◽  
Timothy J. Baroni ◽  
P. Brandon Matheny
Keyword(s):  
North America ◽  
North American ◽  
Phylogenetic Analyses ◽  
Eastern North America ◽  
Phylogenetic Species ◽  
Additional Species ◽  
Taxonomic Descriptions ◽  
Molecular Phylogenetic ◽  
Herbarium Collections ◽  
Environmental Sequences

Five species of Hydnum have been generally recognized from eastern North America based on morphological recognition: H.albidum, H.albomagnum, H.repandum and varieties, H.rufescens, and H.umbilicatum. Other unique North American species, such as H.caespitosum and H.washingtonianum, are either illegitimately named or considered synonymous with European taxa. Here, seventeen phylogenetic species of Hydnum are detected from eastern North America based on a molecular phylogenetic survey of ITS sequences from herbarium collections and GenBank data, including environmental sequences. Based on current distribution results, sixteen of these species appear endemic to North America. Of these, six species are described as new: H.alboaurantiacum, H.cuspidatum, H.ferruginescens, H.subconnatum, H.subtilior, and H.vagabundum. Geographic range extensions and taxonomic notes are provided for five additional species recently described as new from eastern North America. A new name, H.geminum, is proposed for H.caespitosum Banning ex Peck, non Valenti. Overall, species of Hydnum are best recognized by a combination of morphological and molecular phylogenetic analyses. Taxonomic descriptions are provided for seventeen species, including epitype designations for H.albidum, H.albomagnum, and H.umbilicatum, taxa described more than 100 years ago, and molecular annotation of the isotype of H.washingtonianum. Photographs and a key to eastern North American Hydnum species are presented.

Baorangia duplicatopora (Boletaceae, Boletales), a new bolete from tropical China

Phytotaxa ◽  
2021 ◽  
Vol 508 (1) ◽  
Author(s):  
XU ZHANG ◽  
ZHI-QUN LIANG ◽  
SHUAI JIANG ◽  
CHANG XU ◽  
XIN-HUA FU ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Its Region ◽  
Tropical Region ◽  
Translation Elongation ◽  
Line Drawings ◽  
Microscopic Features ◽  
Tropical China

Baorangia duplicatopora is described as a new species from Hainan Province, a tropical region of China. It is morphologically characterized by large to very large basidiomata with a dull rose red, rose pink to purplish red pileus, compound pores, pileus context near hymenophore and stipe context staining blue when injured, a red stipe, and cheilocystidia wider than those of other Baorangia species. Phylogenetic analyses of DNA sequences from part of the 28S gene, the nuc rDNA internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α gene (TEF1) also confirmed that B. duplicatopora forms an independent lineage within Baorangia. Detailed descriptions, color photographs of fresh basidiomata, and line drawings of microscopic features of the new species are presented. A key to species of Baorangia in the world is also provided.

Amanita submelleialba sp. nov. in section Amanita from northern Thailand

Phytotaxa ◽  
2021 ◽  
Vol 513 (2) ◽  
pp. 129-140
Author(s):  
YUAN S. LIU ◽  
JIAN-KUI LIU ◽  
PETER E. MORTIMER ◽  
SAISAMORN LUMYONG
Keyword(s):  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Line Drawing ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Nuclear Rdna ◽  
Internal Transcribed Spacer Region ◽  
Northern Thailand ◽  
Extant Species ◽  
Distinct Lineage

Amanita submelleialba sp. nov. in section Amanita, is described from northern Thailand based on both multi-gene phylogenetic analysis and morphological evidences. It is characterized by having small to medium-sized basidiomata; a yellow to yellowish pale pileus covering pyramidal to subconical, white to yellow white volval remnants; globose stipe base covered conical, white to yellow white volval remnants; fugacious subapical annulus; and absent clamps. Multi-gene phylogenetic analyses based on partial nuclear rDNA internal transcribed spacer region (ITS), partial nuclear rDNA larger subunit region (nrLSU), RNA polymerase II second largest subunit (RPB2), partial translation elongation factor 1-alpha (TEF1-α) and beta-tubulin gene (TUB) indicated that A. submelleialba clustered together with A. elata and A. mira, but represented as a distinct lineage from other extant species in section Amanita. The detailed morphological characteristics, line-drawing illustration and comparisons with morphologically similar taxa are provided.

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