Identification and Characterization of Nothophoma quercina Causing Bud Blight on Photinia fraseri Dress in China

Plant Disease ◽  
2020 ◽  
Author(s):  
Jian Zou ◽  
Yanhan Dong ◽  
Huizheng Wang ◽  
W. X. Liang ◽  
De Long Li
Keyword(s):  
Green Plant ◽  
Morphological Characterization ◽  
Causal Agent ◽  
Molecular Analyses ◽  
First Report ◽  
The World ◽  
Pathogenicity Tests ◽  
Blight Disease ◽  
Identification And Characterization

Photinia (Photinia × fraseri) is a well-known green plant that has high ornamental value and is widely distributed around the world. An outbreak of typical bud blight disease was observed between May and August in photinia in 2017 in Qingdao, China. The causal agent for this blight was subsequently isolated from symptomatic samples and identified as Nothophoma quercina based on morphological characterization and molecular analyses (ITS, LSU, RPB2 and TUB2). Results of pathogenicity tests on isolated fungi also supported the conclusion that N. quercina is the pathogen responsible for this condition. To our knowledge, this is the first report of bud blight on P. fraseri caused by N. quercina in China.

scholarly journals Identification and Characterization of Inorganic Pyrophosphatase and PAP Phosphatase from Thermococcus onnurineus NA1

2009 ◽  
Vol 191 (10) ◽  
pp. 3415-3419 ◽  
Author(s):  
Hyun Sook Lee ◽  
Yun Jae Kim ◽  
Jung-Hyun Lee ◽  
Sung Gyun Kang
Keyword(s):  
Gene Clusters ◽  
Inorganic Pyrophosphatase ◽  
Thermococcus Onnurineus Na1 ◽  
First Report ◽  
Activation System ◽  
Sulfate Activation ◽  
Pyrococcus Abyssi ◽  
Identification And Characterization ◽  
Hypothetical Genes

ABSTRACT Two hypothetical genes were functionally verified to be a pyrophosphatase and a PAP phosphatase in Thermococcus onnurineus NA1. This is the first report of the pyrophosphatases and the PAP phosphatases being organized in the gene clusters of the sulfate activation system only in T. onnurineus NA1 and “Pyrococcus abyssi.”

scholarly journals Coccinonectria pachysandricola, Causal Agent of a New Foliar Blight Disease of Sarcococca hookeriana

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1337-1346 ◽  
Author(s):  
Catalina Salgado-Salazar ◽  
Nina Shiskoff ◽  
Nicholas LeBlanc ◽  
Adnan A. Ismaiel ◽  
Maxton Collins ◽  
...  
Keyword(s):  
Woody Plants ◽  
Causal Agent ◽  
Washington Dc ◽  
Disease Symptoms ◽  
Foliar Blight ◽  
Pathogenicity Tests ◽  
Artificial Inoculations ◽  
Blight Disease ◽  
Genome Assemblies ◽  
Severe Leaf

Woody plants of the Buxaceae, including species of Buxus, Pachysandra, and Sarcococca, are widely grown evergreen shrubs and groundcovers. Severe leaf spot symptoms were observed on S. hookeriana at the U.S. National Arboretum in Washington, DC, in 2016. Affected plants were growing adjacent to P. terminalis exhibiting Volutella blight symptoms. Fungi isolated from both hosts were identical based on morphology and multilocus phylogenetic analysis and were identified as Coccinonectria pachysandricola (Nectriaceae, Hypocreales), causal agent of Volutella blight of Pachysandra species. Pathogenicity tests established that Co. pachysandricola isolated from both hosts caused disease symptoms on P. terminalis and S. hookeriana, but not on B. sempervirens. Artificial inoculations with Pseudonectria foliicola, causal agent of Volutella blight of B. sempervirens, did not result in disease on P. terminalis or S. hookeriana. Wounding enhanced infection by Co. pachysandricola and Ps. foliicola on all hosts tested but was not required for disease development. Genome assemblies were generated for the Buxaceae pathogens that cause Volutella diseases: Co. pachysandricola, Ps. buxi, and Ps. foliicola; these ranged in size from 25.7 to 28.5 Mb. To our knowledge, this foliar blight of S. hookeriana represents a new disease for this host and is capable of causing considerable damage to infected plants.

Identification and characterization of Diaporthe eres causing leaf blight disease on the medicinal herb Polygonatum sibiricum

2020 ◽  
Vol 86 (6) ◽  
pp. 468-476
Author(s):  
Hang Tao ◽  
Hao Wang ◽  
Shen-Xin Huang ◽  
Ye Zhang ◽  
Zi-Hui Zhang ◽  
...  
Keyword(s):  
Leaf Blight ◽  
Medicinal Herb ◽  
Blight Disease ◽  
Identification And Characterization

Molecular Characterization of Potato leafroll virus, Potato virus A, and Potato virus X Isolates from Potatoes in Alaskan Cities and Villages

2011 ◽  
Vol 12 (1) ◽  
pp. 39 ◽  
Author(s):  
Nancy L. Robertson ◽  
Jeffrey Smeenk ◽  
Jodie M. Anderson
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus X ◽  
Potato Leafroll Virus ◽  
First Report ◽  
Potato Virus A ◽  
Potato Viruses ◽  
The World ◽  
Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

scholarly journals Molecular and morphological characterization of the grapevine cultivars “Italia” in the Ica and Cañete valleys (Peru)

2019 ◽  
Vol 12 ◽  
pp. 01017
Author(s):  
K. Mendoza ◽  
M.R. Torres ◽  
G.A. Aliquo ◽  
J.A. Prieto ◽  
M. Grados ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Parentage Analysis ◽  
Morphological Characterization ◽  
Variety Identification ◽  
Morphological Descriptors ◽  
Greenish Yellow ◽  
The World ◽  
Simple Sequence ◽  
Made In

The denomination of “Uva de Italia” or “Italia” is commonly used by growers to refer to a group of varieties used from the colonial times to making a brandy of Pisco with muscat aroma. Previous work have demonstrated that Uva de Italia corresponds in fact to the variety Muscat of Alexandria, a widely spread variety around the world and in South America. However, the distinction between “Italia Dorada” and “Italia Rosada” is usually made, in allusion to the color variations observed in the berries. Our aim was to characterize 5 samples collected in vineyards from the valleys of Ica and Cañete. The genotypes were identified using 13 molecular markers of nuclear simple sequence repeat, and 23 morphological descriptors according to OIV. Our results showed that four genotypes were identified as Muscat of Alexandria while the other corresponded to variety well-known in Argentina as Moscatel Rosado or Uva Pastilla in Chile respectively. Moscatel Rosado showed functionally female flowers, with variable berries size and color in the range from greenish yellow through pink. This is the first identification of Moscatel Rosado as a variety present in the Peruvian vineyards and would allow its use in the pisco industry with distinctive aromatic characteristics. Keywords: Italia, Muscat of Alexandria, Moscatel Rosado, variety identification, parentage analysis, microsatellites, Pisco.

scholarly journals Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1062 ◽  
Author(s):  
Maela León ◽  
Mónica Berbegal ◽  
José M. Rodríguez-Reina ◽  
Georgina Elena ◽  
Paloma Abad-Campos ◽  
...  
Keyword(s):  
New Species ◽  
Sequence Analysis ◽  
Phylogenetic Species ◽  
Shoot Blight ◽  
Pathogenicity Tests ◽  
Almond Orchards ◽  
One Year ◽  
Prevalent Species ◽  
Identification And Characterization

Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS, tub, tef-1α, cal and his), allowed the identification of four known Diaporthe species, namely: D. amygdali, D. eres, D. foeniculina and D. phaseolorum. Moreover, a novel phylogenetic species, D. mediterranea, was described. Diaporthe amygdali was the most prevalent species, due to the largest number of isolates (85.3%) obtained from all sampled regions. The second most frequent species was D. foeniculina (10.2%), followed by D. mediterranea (3.6%), D. eres and D. phaseolorum, each with only one isolate. Pathogenicity tests were performed using one-year-old almond twigs cv. Vayro and representative isolates of the different species. Except for D. foeniculina and D. phaseolorum, all Diaporthe species were able to cause lesions significantly different from those developed on the uninoculated controls. Diaporthe mediterranea caused the most severe symptoms. These results confirm D. amygdali as a key pathogen of almonds in Spain. Moreover, the new species, D. mediterranea, should also be considered as a potential important causal agent of twig cankers and shoot blight on this crop.

Identification and Characterization of the Causal Agent of Sugarcane Root and Basal Stem Rot in South Sumatra, Indonesia

Sugar Tech ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 105-111
Author(s):  
Tri Maryono ◽  
Ani Widiastuti ◽  
Rudi Hari Murti ◽  
Achmadi Priyatmojo
Keyword(s):  
Causal Agent ◽  
Stem Rot ◽  
Basal Stem Rot ◽  
Identification And Characterization
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